##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7716155
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.164141726028056	33.0	31.0	34.0	31.0	34.0
2	32.45395355588373	34.0	31.0	34.0	31.0	34.0
3	32.246551034809436	33.0	31.0	34.0	30.0	34.0
4	36.06886175822025	37.0	35.0	37.0	35.0	37.0
5	35.908070275934065	37.0	35.0	37.0	35.0	37.0
6	36.007745049185765	37.0	35.0	37.0	35.0	37.0
7	35.97475141440264	37.0	35.0	37.0	35.0	37.0
8	35.95180954763091	37.0	35.0	37.0	35.0	37.0
9	37.738182034964304	39.0	37.0	39.0	35.0	39.0
10-11	37.66176515375858	39.0	37.0	39.0	35.0	39.0
12-13	37.635514449878215	39.0	37.0	39.0	35.0	39.0
14-15	38.958079704205005	40.0	38.0	41.0	35.5	41.0
16-17	38.940022524171695	40.0	38.0	41.0	35.0	41.0
18-19	38.91283495471514	40.0	38.0	41.0	35.0	41.0
20-21	38.90647462272375	40.0	38.0	41.0	35.0	41.0
22-23	38.8144581580057	40.0	38.0	41.0	35.0	41.0
24-25	38.74842383912477	40.0	38.0	41.0	34.0	41.0
26-27	38.60110586272757	40.0	38.0	41.0	34.0	41.0
28-29	38.475632817215114	40.0	38.0	41.0	34.0	41.0
30-31	38.32531774729793	40.0	38.0	41.0	34.0	41.0
32-33	38.16776521713532	40.0	38.0	41.0	33.5	41.0
34-35	38.27390400806088	40.0	38.0	41.0	34.0	41.0
36-37	38.26642594510132	40.0	38.0	41.0	34.0	41.0
38-39	38.196671126470356	40.0	38.0	41.0	33.0	41.0
40-41	38.19139626495504	40.0	38.0	41.0	33.0	41.0
42-43	38.26937580225754	40.0	38.0	41.0	33.0	41.0
44-45	38.23293908458045	40.0	38.0	41.0	33.0	41.0
46-47	38.15472966897639	40.0	38.0	41.0	33.0	41.0
48-49	38.06417596639244	40.0	37.0	41.0	33.0	41.0
50-51	37.95385770934223	40.0	37.0	41.0	33.0	41.0
52-53	37.813299404283626	40.0	37.0	41.0	33.0	41.0
54-55	37.658716520599725	40.0	36.5	41.0	32.0	41.0
56-57	37.55335936586731	39.0	36.0	41.0	32.0	41.0
58-59	37.35862440437738	39.0	36.0	41.0	32.0	41.0
60-61	37.170239191240086	39.0	35.5	41.0	31.5	41.0
62-63	36.923866069903426	39.0	35.0	40.0	31.0	41.0
64-65	36.633809231055594	38.0	35.0	40.0	31.0	41.0
66-67	36.358808804915526	38.0	35.0	40.0	31.0	41.0
68-69	36.08657708185456	37.0	35.0	40.0	30.5	41.0
70-71	35.78679998511906	37.0	34.0	39.0	30.0	41.0
72-73	35.32972042983003	36.0	34.0	39.0	29.5	40.5
74-75	35.08405470405587	36.0	34.0	39.0	29.5	40.0
76-77	33.843813269312015	34.5	32.5	37.0	28.5	39.0
78-79	34.560156977334074	35.0	34.0	37.0	29.5	39.0
80-81	34.46650707616504	35.0	34.0	37.0	30.0	39.0
82-83	34.211150679951984	35.0	34.0	36.0	30.0	38.5
84-85	33.971901516160045	35.0	34.0	36.0	30.0	37.0
86-87	33.76469812250391	35.0	33.5	36.0	29.0	37.0
88-89	33.57054562317512	35.0	33.0	35.0	29.0	36.5
90-91	33.40531337366879	35.0	33.0	35.0	29.0	36.0
92-93	33.31395945806918	35.0	33.0	35.0	29.0	36.0
94-95	33.26331085746796	35.0	33.0	35.0	29.0	36.0
96-97	33.30979333947347	35.0	33.0	35.0	29.0	35.5
98-99	33.39820501376323	35.0	33.0	35.0	30.0	35.0
100	32.18226028535852	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	13.0
20	40.0
21	219.0
22	984.0
23	3521.0
24	9280.0
25	19982.0
26	36368.0
27	58283.0
28	84898.0
29	114421.0
30	146353.0
31	183182.0
32	228372.0
33	290888.0
34	390640.0
35	547530.0
36	858639.0
37	1528839.0
38	2037740.0
39	1129125.0
40	46837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06191490450879	20.66478706039472	14.140112530139687	24.13318550495681
2	14.655304358193947	21.233062321842937	41.20228274315381	22.909350576809302
3	18.036509116263215	26.04759754048487	30.75976312036241	25.156130222889512
4	12.333720616032208	18.11244071691147	38.866145120205594	30.68769354685073
5	12.936896679758247	37.94274479970918	36.098043649978514	13.022314870554052
6	29.97477370529752	38.18583219232895	18.625364576009684	13.214029526363843
7	26.46748283309498	32.55524286383568	23.053528603300478	17.923745699768862
8	24.384333907237476	36.88531399382205	21.342015550491144	17.388336548449324
9	25.002517445541205	16.939317574621036	21.48308321955689	36.57508176028087
10-11	23.911514219193368	27.031325835211966	29.066944611662155	19.99021533393251
12-13	24.562790664521383	24.31714370693694	29.20107877563372	21.918986852907956
14-15	21.753172402576155	25.974944774955922	26.559244857056395	25.712637965411528
16-17	21.59805628580556	29.27404905681651	27.27969435554366	21.848200301834268
18-19	21.636527519211317	28.354990276893087	28.814999180291217	21.193483023604372
20-21	22.32119874548083	27.633172124664927	28.661878621459806	21.38375050839444
22-23	21.92862967403152	27.673062482963136	28.562459826048002	21.835848016957346
24-25	21.81961341110166	27.69595938702767	28.75656090036882	21.727866301501848
26-27	21.845576415295746	27.943645757322876	28.606744423550985	21.604033403830396
28-29	21.68652687355134	27.834539769024257	28.70731541741611	21.7716179400083
30-31	21.937040525597588	27.733583122457254	28.70703890968478	21.622337442260378
32-33	21.549444562343144	27.731369748248813	28.86394943715364	21.855236252254407
34-35	21.67458511619575	27.859251687531984	28.685313456414352	21.780849739857917
36-37	21.72558924997939	27.810460107435937	28.74900330821332	21.71494733437136
38-39	21.54711602722803	27.92414982123429	28.78798211048116	21.740752041056517
40-41	21.82939545829728	27.739005361731444	28.742419602001846	21.68917957796943
42-43	21.8282800011009	27.766060614831083	28.640965156713605	21.764694227354404
44-45	21.719209879540006	28.083399417258686	28.397581670027584	21.79980903317372
46-47	22.023489483678862	27.752344135912587	28.071390561724165	22.152775818684386
48-49	21.87715010468083	27.829769447577718	28.20423257056995	22.088847877171506
50-51	21.699686266854048	28.000388181773396	28.29539077966507	22.004534771707483
52-53	21.876393578163427	28.09684829361796	28.253000421963335	21.773757706255278
54-55	21.909229035991817	27.994750679632986	28.092047100840624	22.003973183534576
56-57	22.069242213451624	28.191365341535512	27.82471395140254	21.91467849361032
58-59	21.71652060506251	28.41819454622193	28.202943924988883	21.66234092372668
60-61	21.97074766971975	28.60225443844061	27.78378559181846	21.643212300021176
62-63	21.837468705982165	28.73160201804493	27.715645468009853	21.715283807963047
64-65	21.793668475452353	28.577081117708463	27.84366214582364	21.785588261015544
66-67	21.93346846376249	28.496375943148877	27.70866410227081	21.861491490817826
68-69	21.924449509821198	28.365634528567423	27.865664471359032	21.844251490252343
70-71	21.924707943284314	27.97692074907858	27.980379488071645	22.117991819565457
72-73	22.01002696597396	28.04836981355507	27.918350699248943	22.023252521222027
74-75	21.849574752095	28.20204305467745	27.881206391414548	22.067175801813004
76-77	21.861335291794884	28.206999310439123	27.841129519980186	22.090535877785808
78-79	21.8693491551366	28.17500158083068	27.838074284129465	22.11757497990325
80-81	21.87383869501131	28.179507500363687	27.835617774594517	22.11103603003048
82-83	21.93614437482863	28.134335655202737	28.009187874494607	21.92033209547403
84-85	22.087255107322587	28.07436497123696	27.860455534570182	21.977924386870274
86-87	21.83295471719396	28.20101240894332	27.918477844706246	22.047555029156467
88-89	21.925064307454146	28.146393363175292	27.93313270606016	21.9954096233104
90-91	21.865372745589198	28.225574997165232	27.969640923699203	21.93941133354637
92-93	21.947572128256578	28.21129979236714	27.966561720681344	21.87456635869494
94-95	21.778672716566213	28.293305625148424	27.989454364325066	21.938567293960297
96-97	21.76070207025035	28.419451351162696	28.12237126826603	21.697475310320925
98-99	22.826794444343737	30.346246281312954	28.364123380524177	18.462835893819125
100	21.88674646214315	31.47802054764138	22.08145572901381	24.553777261201663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1491.0
1	1311.0
2	1696.0
3	2434.5
4	3269.0
5	5213.5
6	6964.5
7	8269.0
8	9211.5
9	9081.0
10	8079.5
11	7113.5
12	7668.5
13	10208.0
14	16026.5
15	23840.5
16	30451.5
17	34668.5
18	36011.0
19	35289.0
20	33944.5
21	32790.0
22	33617.5
23	37878.5
24	45615.0
25	57195.0
26	73150.5
27	90893.0
28	108155.5
29	126864.0
30	144671.5
31	160540.0
32	179031.0
33	198708.0
34	218591.0
35	238018.0
36	257563.5
37	274012.5
38	283570.5
39	292825.0
40	303822.5
41	311405.0
42	315575.0
43	321842.0
44	327755.5
45	333095.0
46	340568.5
47	347054.0
48	349419.5
49	344046.5
50	332549.0
51	317300.0
52	297348.5
53	274946.0
54	252406.0
55	229810.0
56	209271.5
57	188960.0
58	166581.5
59	145412.5
60	124163.5
61	102138.0
62	81557.0
63	64122.5
64	50013.0
65	39585.0
66	31550.0
67	23917.5
68	17642.5
69	13541.5
70	9819.0
71	6379.0
72	4503.5
73	3171.0
74	2134.0
75	1360.5
76	858.5
77	545.5
78	346.5
79	234.5
80	160.5
81	97.5
82	65.0
83	44.5
84	27.0
85	20.0
86	16.0
87	9.0
88	3.5
89	3.0
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005404436536945933
22-23	0.004575359936955947
24-25	0.003539080752942671
26-27	0.01700096935351577
28-29	0.02149691876416271
30-31	0.039462114030494315
32-33	0.02383782455074242
34-35	0.01262183210344665
36-37	0.006828535217297885
38-39	0.0026891323199177723
40-41	0.007751574433873777
42-43	0.009074164117009115
44-45	0.0057879881137821425
46-47	0.002101947786510695
48-49	0.010014895758712627
50-51	0.0044880619672016675
52-53	0.0
54-55	4.6142587806100195E-4
56-57	7.071969243787038E-4
58-59	3.762463714357296E-4
60-61	9.108698882093866E-4
62-63	9.366212566496333E-4
64-65	0.0
66-67	2.013554474276803E-4
68-69	5.964976639660235E-4
70-71	2.94190727028773E-4
72-73	6.596644624756306E-4
74-75	2.847534230615777E-4
76-77	0.0
78-79	0.0
80-81	4.8097413970813645E-4
82-83	0.002307684812647617
84-85	4.32787083902268E-5
86-87	6.139920544165369E-4
88-89	2.313692575040169E-4
90-91	0.008679882148900123
92-93	7.81640245387219E-4
94-95	8.437701450122122E-5
96-97	1.5404699261277276E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	185.0
20-21	534.0
22-23	1126.0
24-25	2171.0
26-27	4417.0
28-29	10127.0
30-31	22687.0
32-33	28693.0
34-35	39256.0
36-37	59568.0
38-39	67493.0
40-41	67786.0
42-43	74272.0
44-45	84998.0
46-47	83798.0
48-49	76930.0
50-51	69894.0
52-53	68850.0
54-55	75370.0
56-57	80064.0
58-59	80764.0
60-61	78742.0
62-63	78040.0
64-65	82712.0
66-67	85214.0
68-69	84486.0
70-71	75250.0
72-73	69628.0
74-75	71350.0
76-77	71351.0
78-79	75831.0
80-81	76323.0
82-83	73060.0
84-85	75075.0
86-87	80345.0
88-89	86635.0
90-91	92092.0
92-93	98917.0
94-95	122395.0
96-97	277086.0
98-99	499062.0
100-101	4463578.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.12383156085434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8717997946187	79.17099378938036
2	6.596912253274952	11.494965439521259
3	1.4519896680420668	3.7950870979979348
4	0.5302903975581065	1.8480372510076382
5	0.21920213159916346	0.9548864795612871
6	0.11907189309739649	0.6224399734709775
7	0.06856252834638964	0.41814011197260054
8	0.04168614860278149	0.29054855914315825
9	0.025387385372271486	0.19906616582498504
>10	0.07327218036361446	0.9173067672570208
>50	0.001063617379230681	0.06466042579270873
>100	6.117712728579188E-4	0.10330283359487452
>500	1.287690342851002E-4	0.08012127251961143
>1k	2.1461438186761046E-5	0.040443832955529536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.591964521189634E-5	0.0	0.0	0.0
3	0.0	2.591964521189634E-5	0.0	0.0	0.0
4	0.0	1.0367858084758535E-4	0.0	1.295982260594817E-5	0.0
5	0.0	1.0367858084758535E-4	0.0	1.295982260594817E-5	0.0
6	9.071875824163718E-5	1.0367858084758535E-4	0.0	1.295982260594817E-5	0.0
7	9.071875824163718E-5	1.0367858084758535E-4	0.0	1.295982260594817E-5	0.0
8	9.071875824163718E-5	1.0367858084758535E-4	0.0	1.295982260594817E-5	0.0
9	9.071875824163718E-5	1.5551787127137802E-4	0.0	1.295982260594817E-5	0.0
10-11	9.071875824163718E-5	1.5551787127137802E-4	0.0	1.295982260594817E-5	0.0
12-13	9.071875824163718E-5	1.619977825743521E-4	0.0	1.295982260594817E-5	0.0
14-15	9.071875824163718E-5	2.073571616951707E-4	0.0	1.295982260594817E-5	6.479911302974085E-6
16-17	1.4255804866542988E-4	2.2031698430111888E-4	0.0	1.295982260594817E-5	1.295982260594817E-5
18-19	1.5551787127137802E-4	2.591964521189634E-4	0.0	1.295982260594817E-5	1.295982260594817E-5
20-21	1.5551787127137802E-4	3.1103574254275605E-4	0.0	1.295982260594817E-5	1.295982260594817E-5
22-23	1.5551787127137802E-4	3.758348555724969E-4	0.0	1.9439733908922253E-5	1.295982260594817E-5
24-25	1.684776938773262E-4	4.2767414599628956E-4	0.0	7.127902433271493E-5	1.295982260594817E-5
26-27	1.684776938773262E-4	4.4063396860223776E-4	0.0	1.295982260594817E-4	1.295982260594817E-5
28-29	1.684776938773262E-4	4.4063396860223776E-4	0.0	6.285513963884863E-4	1.295982260594817E-5
30-31	1.749576051803003E-4	5.183929042379268E-4	0.0	0.0020476519717398107	1.295982260594817E-5
32-33	1.8143751648327437E-4	5.37832638146849E-4	0.0	0.005209848687591164	1.9439733908922253E-5
34-35	2.0087725039219664E-4	6.674308642063307E-4	0.0	0.009117235203284538	2.591964521189634E-5
36-37	2.073571616951707E-4	8.164688241747346E-4	0.0	0.014702918746448197	2.591964521189634E-5
38-39	2.073571616951707E-4	8.553482919925792E-4	0.0	0.023385999892433472	2.591964521189634E-5
40-41	2.073571616951707E-4	9.460670502342164E-4	0.0	0.031783964941087886	2.591964521189634E-5
42-43	2.2031698430111888E-4	0.0010043862519609832	0.0	0.03995513309413821	2.591964521189634E-5
44-45	2.2679689560409298E-4	0.0011599041232323612	0.0	0.049007569184393	2.591964521189634E-5
46-47	2.3327680690706705E-4	0.0012571027927769725	0.0	0.0593106281561218	2.591964521189634E-5
48-49	2.3327680690706705E-4	0.0012635827040799465	0.0	0.06915361342533943	3.239955651487042E-5
50-51	2.591964521189634E-4	0.0013543014623215838	0.0	0.0782125294268972	3.8879467817844506E-5
52-53	2.591964521189634E-4	0.0013607813736245579	0.0	0.0865392154512189	5.183929042379268E-5
54-55	2.591964521189634E-4	0.0013607813736245579	0.0	0.09483998183032871	5.183929042379268E-5
56-57	2.591964521189634E-4	0.0013607813736245579	0.0	0.10319906741116527	5.183929042379268E-5
58-59	2.591964521189634E-4	0.0013607813736245579	0.0	0.1106963247887063	5.183929042379268E-5
60-61	2.6567636342193746E-4	0.001393180930139428	0.0	0.11766222943940344	5.183929042379268E-5
62-63	2.980759199368079E-4	0.0014191005753513247	0.0	0.12442077692840542	5.183929042379268E-5
64-65	3.1103574254275605E-4	0.0015940581805316248	0.0	0.13120524406261927	5.183929042379268E-5
66-67	3.2399556514870425E-4	0.0016329376483494695	0.0	0.13780827368034987	5.183929042379268E-5
68-69	3.2399556514870425E-4	0.0016912568500762363	0.0	0.14386699074863063	5.831920172676677E-5
70-71	3.434352990576265E-4	0.0017171764952881325	0.0	0.14905739970231285	6.479911302974085E-5
72-73	3.7583485557249693E-4	0.0017236564065911065	0.0	0.15325638222664006	6.479911302974085E-5
74-75	3.7583485557249693E-4	0.001749576051803003	0.0	0.15707304998409183	6.479911302974085E-5
76-77	3.7583485557249693E-4	0.0017949354309238215	0.0	0.16059164182160673	6.479911302974085E-5
78-79	3.7583485557249693E-4	0.0018143751648327438	0.0	0.16313824696367557	6.479911302974085E-5
80-81	3.7583485557249693E-4	0.0018143751648327438	0.0	0.16418799259475736	6.479911302974085E-5
82-83	3.8231476687547103E-4	0.0018208550761357179	0.0	0.16471934532160126	6.479911302974085E-5
84-85	3.887946781784451E-4	0.00184029481004464	0.0	0.16492670248329638	6.479911302974085E-5
86-87	3.887946781784451E-4	0.0018921341004684328	0.0	0.16502390115284102	6.479911302974085E-5
88	3.887946781784451E-4	0.0020217323265279146	0.0	0.16504334088674993	6.479911302974085E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8295	0.0	42.936447	1
CTTATAC	7345	0.0	35.326702	1
GTATCAA	13670	0.0	32.159435	1
TATACAC	10015	0.0	28.71399	3
TCAACGC	15890	0.0	26.990362	4
ATCAACG	15905	0.0	26.860996	3
CAACGCA	16330	0.0	26.187222	5
AACGCAG	16950	0.0	25.253717	6
TATCAAC	17455	0.0	24.617771	2
TTATACA	11565	0.0	23.829529	2
ACATCTC	11985	0.0	22.236013	8
ACACATC	12085	0.0	21.91526	6
ACGCAGA	19760	0.0	21.746115	7
TACACAT	13550	0.0	21.43637	5
CGCAGAG	20140	0.0	21.33581	8
TACATGG	20045	0.0	20.365082	2
GTACATG	20785	0.0	20.335781	1
CACATCT	13285	0.0	19.749105	7
ACATGGG	20245	0.0	19.286316	3
CATCTCC	14115	0.0	18.704893	9
>>END_MODULE