FastQCFastQC Report
Fri 27 May 2016
SRR2140202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195495
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC66300.050.8694041
GGTATCA40650.043.231121
TATACAC82500.042.6514973
TTATACA84900.040.10732
GTATCAA73200.034.153781
TACACAT111300.031.6879235
ACACATC116300.029.3833666
TCAACGC84950.028.9997634
ATCAACG85400.028.7994293
CAACGCA86650.028.3839765
ACATCTC121150.028.006068
AACGCAG90000.027.3725576
TATCAAC91800.027.0126822
ATACACA135250.026.9468634
CACATCT129850.026.3484337
CATCTCC138650.024.4712149
ACGCAGA105500.023.2740557
CGCAGAG109600.022.4774638
GTACATG105100.021.3546051
TACATGG104950.021.1531012
Produced by FastQC (version 0.11.5)