##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4195495
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16266519206911	33.0	31.0	34.0	31.0	34.0
2	32.45477255961454	34.0	31.0	34.0	31.0	34.0
3	32.250152127460524	33.0	31.0	34.0	30.0	34.0
4	36.07093441894222	37.0	35.0	37.0	35.0	37.0
5	35.90741783746614	37.0	35.0	37.0	35.0	37.0
6	36.00454177635773	37.0	35.0	37.0	35.0	37.0
7	35.96820613539046	37.0	35.0	37.0	35.0	37.0
8	35.945082523039595	37.0	35.0	37.0	35.0	37.0
9	37.7328150790312	39.0	37.0	39.0	35.0	39.0
10-11	37.65649714753563	39.0	37.0	39.0	35.0	39.0
12-13	37.62564548402513	39.0	37.0	39.0	35.0	39.0
14-15	38.94349820462186	40.0	38.0	41.0	35.5	41.0
16-17	38.917910997391246	40.0	38.0	41.0	35.0	41.0
18-19	38.8911257193728	40.0	38.0	41.0	35.0	41.0
20-21	38.87780708695665	40.0	38.0	41.0	35.0	41.0
22-23	38.78200946386044	40.0	38.0	41.0	34.5	41.0
24-25	38.714497825189824	40.0	38.0	41.0	34.0	41.0
26-27	38.55299161706968	40.0	38.0	41.0	34.0	41.0
28-29	38.420727942976995	40.0	38.0	41.0	34.0	41.0
30-31	38.267250099432545	40.0	38.0	41.0	34.0	41.0
32-33	38.11401187978954	40.0	38.0	41.0	33.5	41.0
34-35	38.22120424605947	40.0	38.0	41.0	34.0	41.0
36-37	38.20747964600021	40.0	38.0	41.0	33.5	41.0
38-39	38.12630848740517	40.0	38.0	41.0	33.0	41.0
40-41	38.130992335220064	40.0	38.0	41.0	33.0	41.0
42-43	38.21839788754322	40.0	38.0	41.0	33.0	41.0
44-45	38.18567373377844	40.0	38.0	41.0	33.0	41.0
46-47	38.10711372397911	40.0	37.5	41.0	33.0	41.0
48-49	38.010357081798816	40.0	37.0	41.0	33.0	41.0
50-51	37.86958787979729	40.0	37.0	41.0	33.0	41.0
52-53	37.72896270188909	40.0	37.0	41.0	33.0	41.0
54-55	37.5588570248856	39.0	36.0	41.0	32.0	41.0
56-57	37.42760580669604	39.0	36.0	41.0	32.0	41.0
58-59	37.226669949737044	39.0	35.5	41.0	31.5	41.0
60-61	37.02015180614023	39.0	35.0	40.5	31.0	41.0
62-63	36.76722434706031	38.5	35.0	40.0	31.0	41.0
64-65	36.48244799154473	38.0	35.0	40.0	31.0	41.0
66-67	36.21125789130883	37.0	35.0	40.0	31.0	41.0
68-69	35.94830514331434	37.0	34.5	39.5	30.0	41.0
70-71	35.655421160010576	36.5	34.0	39.0	30.0	41.0
72-73	35.20453597706503	36.0	34.0	39.0	29.0	40.5
74-75	34.96874482284922	35.5	34.0	38.5	29.0	40.0
76-77	33.74612378996183	34.5	32.5	36.5	28.5	39.0
78-79	34.4592759038988	35.0	34.0	37.0	29.5	39.0
80-81	34.3763000454451	35.0	34.0	37.0	30.0	39.0
82-83	34.135757711079734	35.0	34.0	36.0	30.0	38.5
84-85	33.914757529862115	35.0	34.0	36.0	30.0	37.0
86-87	33.72090840091757	35.0	33.5	35.5	29.5	37.0
88-89	33.539961160133515	35.0	33.0	35.0	29.0	36.5
90-91	33.38615378975297	35.0	33.0	35.0	29.0	36.0
92-93	33.3014185737195	35.0	33.0	35.0	29.0	36.0
94-95	33.25519752724608	35.0	33.0	35.0	29.0	36.0
96-97	33.30360722423928	35.0	33.0	35.0	29.0	35.0
98-99	33.397761708678715	35.0	33.0	35.0	30.0	35.0
100	32.233411737595176	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	25.0
21	109.0
22	617.0
23	2026.0
24	5251.0
25	11395.0
26	20432.0
27	32412.0
28	47073.0
29	62464.0
30	80688.0
31	100030.0
32	124535.0
33	158673.0
34	214177.0
35	301179.0
36	472085.0
37	833122.0
38	1086818.0
39	616272.0
40	26102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.739793516617226	20.46938442305378	13.95299005242528	24.837832007903714
2	14.690972102219165	20.770373936806028	41.088739231008496	23.44991472996631
3	17.993776658058227	25.762061449244968	30.95589435811507	25.28826753458174
4	12.470256787339753	18.115693142287142	37.555711542976454	31.858338527396647
5	13.118404383749713	37.57385004629966	35.86399221069266	13.443753359257965
6	30.393052548030685	37.428217647738826	18.290690371457956	13.888039432772533
7	26.558344128642748	32.25166517895982	22.36203356218992	18.82795713020752
8	24.713293663798908	36.52615483989374	21.22672056574969	17.53383093055766
9	24.79230698642234	16.653648735131373	20.811298785959703	37.742745492486584
10-11	24.21406770833954	26.48385947307767	28.64356887566306	20.658503942919726
12-13	24.8961445550525	23.713232884319968	28.71407307123474	22.676549489392787
14-15	22.060150232570887	25.483727188329386	25.69023440619045	26.765888172909275
16-17	22.177049430400942	28.953139021736412	26.45762895677387	22.412182591088776
18-19	22.374392056241277	27.63142370566524	27.971133322766445	22.023050915327037
20-21	22.73307711395882	26.961543018133284	27.88163961647664	22.42374025143125
22-23	22.314373092184365	27.37410070656152	27.885585117497424	22.42594108375669
24-25	22.49686452357449	27.307448365975667	27.929882163311824	22.26580494713801
26-27	22.532354222751355	27.570520140270315	27.943406158291133	21.95371947868719
28-29	22.088681023423305	27.110386160667915	28.170882858636176	22.6300499572726
30-31	22.289134356564176	27.121583306783698	28.37969520337063	22.2095871332815
32-33	21.696036379060242	26.926522469384263	28.857959827773648	22.519481323781847
34-35	21.809103660936856	27.17684737245683	28.414379068266733	22.599669898339585
36-37	22.02889100107372	27.34015847208495	28.47246582700042	22.15848469984091
38-39	22.039980664664995	27.048023385522175	28.41117919226136	22.500816757551473
40-41	22.143207293951978	26.854702661669354	28.595380142662062	22.406709901716606
42-43	22.434951879201233	26.80121442140403	28.570122695923605	22.19371100347113
44-45	21.947616024293605	27.319459703657696	28.372378044768226	22.360546227280473
46-47	22.656145391466353	26.803648547426885	27.634320634335594	22.905885426771164
48-49	22.354989279179186	26.84942250494613	27.92963485513493	22.86595336073976
50-51	22.06637097854574	27.20653298846717	28.155918932966518	22.571177100020574
52-53	22.162722347394933	27.422058863046434	28.439339933504105	21.97587885605453
54-55	22.069196967023704	27.508924180144078	27.812599311874962	22.609279540957257
56-57	22.538814885918825	27.949701457337362	27.16172024910855	22.349763407635265
58-59	21.850023245232663	28.39098949271443	28.007656475923635	21.751330786129273
60-61	22.453285448495475	28.722922219936315	27.016899084045942	21.80689324752227
62-63	21.98738498384358	29.10038032879168	27.022666370261124	21.889568317103617
64-65	22.069082990463336	28.7302937117275	27.132981391709933	22.06764190609923
66-67	22.15448105407337	28.380089796932452	27.19037504071779	22.275054108276393
68-69	22.262182193585982	27.993385482812585	27.452171929680002	22.292260393921424
70-71	22.286608701139176	27.541184475933367	27.566455027064972	22.605751795862485
72-73	22.443552482879134	27.56520931788305	27.456117212603715	22.5351209866341
74-75	22.254330446757944	27.716680319493264	27.50344407661625	22.52554515713254
76-77	22.406844291701606	27.627376220835114	27.43162322214544	22.534156265317836
78-79	22.313625532833946	27.605478916357452	27.443176626425497	22.63771892438311
80-81	22.3864442344125	27.62862233552571	27.472205418586576	22.512728011475218
82-83	22.380072045420523	27.605624360507754	27.614908140692158	22.39939545337957
84-85	22.50147730678518	27.577065268221766	27.480088636741133	22.441368788251918
86-87	22.330338701286934	27.558577745046968	27.62441129774095	22.486672255925146
88-89	22.368445233353686	27.485300267845563	27.61004296349348	22.536211535307267
90-91	22.435840742766956	27.54419879363293	27.602507118125047	22.417453345475074
92-93	22.462645267415578	27.421820778097732	27.654722135543818	22.46081181894287
94-95	22.275839087385155	27.54409885207257	27.717183734890504	22.462878325651776
96-97	22.31975573695864	27.60396807939744	27.8261119611139	22.250164222530024
98-99	23.398525438760995	29.618322361066866	27.999056595275277	18.98409560489686
100	29.984392857724778	40.98318013272472	29.0324270095505	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	516.5
2	799.5
3	1256.0
4	1655.5
5	2624.0
6	3614.5
7	4429.5
8	5021.5
9	5028.0
10	4523.5
11	3974.0
12	4234.5
13	5467.0
14	8437.5
15	12529.5
16	15884.0
17	18064.0
18	18908.0
19	18613.0
20	17963.0
21	17706.0
22	18356.0
23	20318.5
24	23996.5
25	29648.0
26	36882.0
27	45147.5
28	54141.0
29	64233.5
30	73813.5
31	82489.0
32	91513.0
33	100620.5
34	109266.0
35	118037.0
36	126542.5
37	134349.5
38	141479.0
39	145928.5
40	150533.5
41	157223.5
42	161934.5
43	165782.0
44	171228.0
45	177224.5
46	188333.0
47	200585.5
48	209306.0
49	208762.0
50	200238.5
51	191534.0
52	180698.0
53	168854.0
54	156314.5
55	144233.0
56	132437.5
57	120211.0
58	107599.0
59	93220.0
60	78430.5
61	64837.0
62	52118.0
63	41121.5
64	31822.5
65	24252.5
66	18580.5
67	14379.0
68	10985.5
69	8232.0
70	6070.5
71	4330.5
72	3063.0
73	2089.0
74	1402.5
75	958.5
76	642.5
77	418.5
78	278.5
79	182.0
80	126.5
81	93.5
82	67.5
83	48.0
84	30.5
85	20.5
86	16.5
87	12.5
88	8.0
89	5.0
90	5.0
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0052320173240555405
22-23	0.004719961467950925
24-25	0.0035526189358404173
26-27	0.016493915855984143
28-29	0.02103097114865865
30-31	0.03854442475166771
32-33	0.022786929014336325
34-35	0.012232079792206491
36-37	0.006998346231354168
38-39	0.002484790754913704
40-41	0.007491700716199171
42-43	0.008903066088595871
44-45	0.005973522519295867
46-47	0.0019950157855624035
48-49	0.00986409810039736
50-51	0.004282051812565033
52-53	1.3237227531527104E-5
54-55	4.14983029202088E-4
56-57	5.69592314301039E-4
58-59	4.404050735765489E-4
60-61	9.783349131112812E-4
62-63	0.0011065432312963338
64-65	0.0
66-67	3.2266131048891254E-4
68-69	6.418631825337982E-4
70-71	2.426937796067451E-4
72-73	7.37830409680335E-4
74-75	2.9572822140642736E-4
76-77	0.0
78-79	1.5974632283933117E-5
80-81	5.022164757137023E-4
82-83	0.0018731524185766114
84-85	3.3319566798984216E-5
86-87	6.762517462088059E-4
88-89	2.9197571723834937E-4
90-91	0.008563738683631025
92-93	8.188149433727166E-4
94-95	1.8169730355750604E-5
96-97	2.4273879569067683E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	119.0
20-21	310.0
22-23	664.0
24-25	1449.0
26-27	2653.0
28-29	6199.0
30-31	13475.0
32-33	16611.0
34-35	23392.0
36-37	35527.0
38-39	40523.0
40-41	40207.0
42-43	43190.0
44-45	49125.0
46-47	47922.0
48-49	45380.0
50-51	41520.0
52-53	41024.0
54-55	46467.0
56-57	52471.0
58-59	55747.0
60-61	54104.0
62-63	54181.0
64-65	58705.0
66-67	59401.0
68-69	56705.0
70-71	45510.0
72-73	40483.0
74-75	41017.0
76-77	40748.0
78-79	43273.0
80-81	43980.0
82-83	41618.0
84-85	43080.0
86-87	45518.0
88-89	49208.0
90-91	51737.0
92-93	55167.0
94-95	64773.0
96-97	146579.0
98-99	837289.0
100-101	1718444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.53495735296916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.27011172537925	82.6140051828553
2	5.839101552307653	10.456074169310433
3	1.1583052903198034	3.111264443115065
4	0.3768591444526982	1.349682697065951
5	0.15496785378184885	0.6937520089719498
6	0.06791728414583373	0.3648582683716026
7	0.04170170265678722	0.26136321182451516
8	0.0241975363648579	0.17332203091795778
9	0.01554748710378695	0.1252839235305062
>10	0.04967443117008027	0.6712152659307845
>50	8.521934773621195E-4	0.052582875451090826
>100	7.637988400682736E-4	0.12659592265485692
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3835089780824432E-5
2	0.0	2.3835089780824432E-5	0.0	0.0	2.3835089780824432E-5
3	0.0	2.3835089780824432E-5	0.0	0.0	2.3835089780824432E-5
4	0.0	2.3835089780824432E-5	0.0	0.0	2.3835089780824432E-5
5	0.0	2.3835089780824432E-5	0.0	0.0	2.3835089780824432E-5
6	0.0	2.3835089780824432E-5	0.0	0.0	4.7670179561648864E-5
7	0.0	2.3835089780824432E-5	0.0	2.3835089780824432E-5	4.7670179561648864E-5
8	0.0	4.7670179561648864E-5	0.0	2.3835089780824432E-5	4.7670179561648864E-5
9	0.0	1.4301053868494659E-4	0.0	2.3835089780824432E-5	4.7670179561648864E-5
10-11	0.0	1.4301053868494659E-4	0.0	5.958772445206108E-5	4.7670179561648864E-5
12-13	0.0	1.4301053868494659E-4	0.0	7.150526934247329E-5	4.7670179561648864E-5
14-15	0.0	3.2177371204112986E-4	0.0	7.150526934247329E-5	1.0725790401370995E-4
16-17	0.0	3.3369125693154207E-4	0.0	7.150526934247329E-5	1.1917544890412216E-4
18-19	0.0	3.694438916027787E-4	0.0	9.534035912329773E-5	1.4301053868494659E-4
20-21	0.0	4.0519652627401533E-4	0.0	1.549280835753588E-4	1.4301053868494659E-4
22-23	0.0	4.2903161605483976E-4	0.0	1.9068071824659546E-4	1.4301053868494659E-4
24-25	0.0	4.4094916094525197E-4	0.0	2.3835089780824433E-4	1.4301053868494659E-4
26-27	0.0	4.886193405069009E-4	0.0	3.098561671507176E-4	1.4301053868494659E-4
28-29	0.0	5.005368853973131E-4	0.0	8.81898321890504E-4	1.4301053868494659E-4
30-31	0.0	5.124544302877252E-4	0.0	0.0024788493372057407	1.4301053868494659E-4
32-33	0.0	5.243719751781375E-4	0.0	0.006018360169658169	1.4301053868494659E-4
34-35	0.0	6.197123343014352E-4	0.0	0.010356346509768216	1.6684562846577103E-4
36-37	0.0	7.269702383151451E-4	0.0	0.016791820750590814	1.6684562846577103E-4
38-39	0.0	7.388877832055574E-4	0.0	0.026909816362550784	1.7876317335618325E-4
40-41	0.0	7.627228729863818E-4	0.0	0.0369682242500587	1.9068071824659546E-4
42-43	0.0	7.627228729863818E-4	0.0	0.046192403995237746	1.9068071824659546E-4
44-45	0.0	8.103930525480307E-4	0.0	0.05635806978675937	1.9068071824659546E-4
46-47	0.0	8.580632321096795E-4	0.0	0.06869272874833601	1.9068071824659546E-4
48-49	0.0	8.699807770000917E-4	0.0	0.07928742615591247	2.1451580802741988E-4
50-51	0.0	9.772386810138018E-4	0.0	0.09000129901239307	2.1451580802741988E-4
52-53	0.0	0.0010725790401370995	0.0	0.10025038761814756	2.1451580802741988E-4
54-55	0.0	0.0010725790401370995	0.0	0.10942689718376497	2.1451580802741988E-4
56-57	0.0	0.0010725790401370995	0.0	0.11798369441508094	2.2643335291783212E-4
58-59	0.0	0.0010725790401370995	0.0	0.12627830565880782	2.3835089780824433E-4
60-61	0.0	0.0010964141299179238	0.0	0.13400087474779496	2.3835089780824433E-4
62-63	0.0	0.0011679193992603972	0.0	0.1413778350349601	2.6218598758906875E-4
64-65	0.0	0.0012990123930549315	0.0	0.14938642520131712	2.6218598758906875E-4
66-67	0.0	0.0013109299379453437	0.0	0.1570851592005234	2.6218598758906875E-4
68-69	0.0	0.0013347650277261683	0.0	0.16424760367966118	2.6218598758906875E-4
70-71	0.0	0.0013347650277261683	0.0	0.17086184109383995	2.6218598758906875E-4
72-73	0.0	0.0013347650277261683	0.0	0.17596255030693636	2.6218598758906875E-4
74-75	0.0	0.0013586001175069926	0.0	0.1801932787430327	2.6218598758906875E-4
76-77	0.0	0.0014181878419590537	0.0	0.18375662466526596	2.6218598758906875E-4
78-79	0.0	0.0014539404766302903	0.0	0.18616396873312924	2.6218598758906875E-4
80-81	0.0	0.0014539404766302903	0.0	0.1870935372345814	2.7410353247948096E-4
82-83	0.0	0.0014658580215207025	0.0	0.18765366184443077	2.8602107736989317E-4
84-85	0.0	0.0014777755664111148	0.0	0.1879873531013623	2.8602107736989317E-4
86-87	0.0	0.0015611983806440004	0.0	0.18805885837070477	2.8602107736989317E-4
88	0.0	0.001596951015315237	0.0	0.18805885837070477	2.8602107736989317E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6630	0.0	50.869404	1
GGTATCA	4065	0.0	43.23112	1
TATACAC	8250	0.0	42.651497	3
TTATACA	8490	0.0	40.1073	2
GTATCAA	7320	0.0	34.15378	1
TACACAT	11130	0.0	31.687923	5
ACACATC	11630	0.0	29.383366	6
TCAACGC	8495	0.0	28.999763	4
ATCAACG	8540	0.0	28.799429	3
CAACGCA	8665	0.0	28.383976	5
ACATCTC	12115	0.0	28.00606	8
AACGCAG	9000	0.0	27.372557	6
TATCAAC	9180	0.0	27.012682	2
ATACACA	13525	0.0	26.946863	4
CACATCT	12985	0.0	26.348433	7
CATCTCC	13865	0.0	24.471214	9
ACGCAGA	10550	0.0	23.274055	7
CGCAGAG	10960	0.0	22.477463	8
GTACATG	10510	0.0	21.354605	1
TACATGG	10495	0.0	21.153101	2
>>END_MODULE