FastQCFastQC Report
Fri 27 May 2016
SRR2140201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7889019
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77750.044.9078751
GTATCAA132400.033.1429861
TCAACGC154300.027.8792444
ATCAACG154500.027.7100583
CAACGCA159050.026.969065
TATCAAC164100.026.289492
AACGCAG164550.026.1925986
CTTATAC63850.025.8284681
ACGCAGA193000.022.288957
TATACAC87450.021.8679643
CGCAGAG197250.021.7461558
GTACATG192450.020.2370531
TACATGG190850.020.040382
ACATGGG195600.019.301413
TTATACA95550.019.0242182
GCAGAGT240050.017.7832379
CATGGGG151100.017.41934
ACATCTC101450.017.3908358
GAGTACT137100.017.12828412-13
TACACAT112650.016.866545
Produced by FastQC (version 0.11.5)