##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7889019
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.170582045752454	33.0	31.0	34.0	31.0	34.0
2	32.46215708188813	34.0	31.0	34.0	31.0	34.0
3	32.25899012285304	33.0	31.0	34.0	30.0	34.0
4	36.074133298449404	37.0	35.0	37.0	35.0	37.0
5	35.91396484150945	37.0	35.0	37.0	35.0	37.0
6	36.01061386719946	37.0	35.0	37.0	35.0	37.0
7	35.977937180782554	37.0	35.0	37.0	35.0	37.0
8	35.95465038175215	37.0	35.0	37.0	35.0	37.0
9	37.74262947015339	39.0	37.0	39.0	35.0	39.0
10-11	37.66672434177177	39.0	37.0	39.0	35.0	39.0
12-13	37.64067148272808	39.0	37.0	39.0	35.0	39.0
14-15	38.97033027807387	40.0	38.0	41.0	35.5	41.0
16-17	38.95073164356684	40.0	38.0	41.0	35.0	41.0
18-19	38.91923171943178	40.0	38.0	41.0	35.0	41.0
20-21	38.91223332687052	40.0	38.0	41.0	35.0	41.0
22-23	38.81989690679441	40.0	38.0	41.0	35.0	41.0
24-25	38.757244533165604	40.0	38.0	41.0	34.0	41.0
26-27	38.610296747893244	40.0	38.0	41.0	34.0	41.0
28-29	38.48975299975248	40.0	38.0	41.0	34.0	41.0
30-31	38.3394470056993	40.0	38.0	41.0	34.0	41.0
32-33	38.18241847364103	40.0	38.0	41.0	33.5	41.0
34-35	38.28520333769529	40.0	38.0	41.0	34.0	41.0
36-37	38.279232494663624	40.0	38.0	41.0	34.0	41.0
38-39	38.212000613010034	40.0	38.0	41.0	33.0	41.0
40-41	38.19550035522221	40.0	38.0	41.0	33.0	41.0
42-43	38.26683194486868	40.0	38.0	41.0	33.0	41.0
44-45	38.22758510450252	40.0	38.0	41.0	33.0	41.0
46-47	38.14646818029512	40.0	38.0	41.0	33.0	41.0
48-49	38.05651155448693	40.0	37.0	41.0	33.0	41.0
50-51	37.94359606933155	40.0	37.0	41.0	33.0	41.0
52-53	37.79811597671626	40.0	37.0	41.0	33.0	41.0
54-55	37.63926112571451	40.0	36.0	41.0	32.0	41.0
56-57	37.527738582617815	39.0	36.0	41.0	32.0	41.0
58-59	37.32435176021605	39.0	36.0	41.0	32.0	41.0
60-61	37.125590350721836	39.0	35.0	41.0	31.0	41.0
62-63	36.86520303476718	38.5	35.0	40.0	31.0	41.0
64-65	36.56285620256053	38.0	35.0	40.0	31.0	41.0
66-67	36.2781958643482	37.5	35.0	40.0	31.0	41.0
68-69	36.00222409809733	37.0	34.5	40.0	30.0	41.0
70-71	35.69389175733434	36.5	34.0	39.0	30.0	41.0
72-73	35.23758053155997	36.0	34.0	39.0	29.5	40.5
74-75	34.99319827690747	35.5	34.0	38.5	29.5	40.0
76-77	33.760321796726835	34.5	32.5	36.5	28.5	39.0
78-79	34.47016161124102	35.0	34.0	37.0	29.5	39.0
80-81	34.38381441572611	35.0	34.0	37.0	30.0	39.0
82-83	34.14363607026185	35.0	34.0	36.0	30.0	38.5
84-85	33.9194874318071	35.0	34.0	36.0	30.0	37.0
86-87	33.721003076021105	35.0	33.5	35.5	29.5	37.0
88-89	33.54116046584707	35.0	33.0	35.0	29.0	36.5
90-91	33.38506640376606	35.0	33.0	35.0	29.0	36.0
92-93	33.29981817609629	35.0	33.0	35.0	29.0	36.0
94-95	33.25873981755819	35.0	33.0	35.0	29.0	36.0
96-97	33.30358722558316	35.0	33.0	35.0	29.0	35.0
98-99	33.392010906124234	35.0	33.0	35.0	30.0	35.0
100	32.193976604997566	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	38.0
21	216.0
22	1078.0
23	3585.0
24	9536.0
25	20653.0
26	37271.0
27	60172.0
28	86815.0
29	116574.0
30	149299.0
31	187104.0
32	233006.0
33	297130.0
34	399395.0
35	562163.0
36	889980.0
37	1578410.0
38	2051470.0
39	1156214.0
40	48904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.635075286293514	20.140717115778273	13.83137751347791	24.3928300844503
2	14.998506151398544	21.016732245162547	40.614542822117684	23.370218781321224
3	18.40535559617742	25.516176345880265	30.407659051144382	25.670809006797928
4	12.74247152909633	17.705255875286902	38.185368801875114	31.366903793741656
5	13.363448611291215	37.546962429675986	35.70824205138814	13.381346907644664
6	30.771050240847437	37.43566849059433	18.378571530883626	13.414709737674608
7	27.0240824619639	32.1946619725469	22.72435901092392	18.056896554565277
8	24.757209990240867	36.611497576568134	21.00878448892061	17.622507944270385
9	25.24714416329838	16.826959093392983	21.134161294325697	36.79173544898295
10-11	24.378183142923092	26.652470985302486	28.718653105031184	20.250692766743242
12-13	24.94048372807823	23.944149456351923	28.959868140766297	22.155498674803546
14-15	22.027390224310526	25.593549717651843	26.262910508898507	26.116149549139127
16-17	21.970843269613116	28.84425807568723	26.96425246282206	22.220646191877595
18-19	22.041016760132027	27.987275730987594	28.471575489931006	21.500132018949376
20-21	22.619800234801627	27.21860726201552	28.368484910870585	21.793107592312268
22-23	22.33184122568183	27.275193706067807	28.22362952861941	22.16933553963095
24-25	22.22775991500258	27.299797612509806	28.336233066801313	22.1362094056863
26-27	22.23638508084543	27.51770977547637	28.233148041277712	22.01275710240049
28-29	22.13824122664407	27.511360319458873	28.273143837876553	22.077254616020504
30-31	22.337161268334576	27.376724766832115	28.249362659285882	22.036751305547426
32-33	22.008937979868012	27.39365579659725	28.383742424471542	22.213663799063195
34-35	22.129355538989103	27.500795078789043	28.24534375977832	22.124505622443543
36-37	22.180496490655415	27.483813338242445	28.27058616960878	22.06510400149336
38-39	22.04755887507672	27.50987382558226	28.309819673080934	22.132747626260088
40-41	22.308459161643547	27.34190012975916	28.22321858526733	22.126422123329963
42-43	22.248871386072206	27.408298585419278	28.119433743458373	22.22339628505014
44-45	22.185075270993334	27.617131677412804	27.975733150431747	22.222059901162112
46-47	22.414545489079725	27.47831224733518	27.65044399816438	22.456698265420716
48-49	22.29861168602283	27.520498777617558	27.724165114919874	22.456724421439738
50-51	22.219861441040116	27.65994117050524	27.772902352020612	22.34729503643404
52-53	22.352946209363598	27.660048967903805	27.72940800901261	22.25759681371999
54-55	22.31309937399179	27.572148167302895	27.645549761637984	22.46920269706733
56-57	22.3923815861322	27.785275517122322	27.491041135663107	22.33130176108237
58-59	22.22624223814358	27.940258999021356	27.70110716318936	22.132391599645704
60-61	22.423737638330323	28.026817850247703	27.43428347626729	22.115161035154678
62-63	22.293858809171493	28.16927899872962	27.364584770641333	22.17227742145756
64-65	22.287966557019484	28.04583195606568	27.387565110931718	22.278636375983123
66-67	22.329361093096516	27.95887707146762	27.356389591239942	22.35537224419592
68-69	22.29089251932207	27.897707622415858	27.470933062673442	22.340466795588632
70-71	22.328559994694984	27.623028616779333	27.53400364727573	22.514407741249958
72-73	22.410258434404152	27.664823424081487	27.422996908272847	22.50192123324152
74-75	22.247614901293524	27.7773656023638	27.515954597323024	22.459064899019655
76-77	22.403796955933572	27.686348109178233	27.423577233528086	22.48627770136011
78-79	22.291622148643757	27.727999354089565	27.41521459461597	22.56516390265071
80-81	22.27240229980835	27.773277226897758	27.458145154570452	22.49617531872344
82-83	22.301773792157324	27.740339509303396	27.54595873192048	22.4119279666188
84-85	22.413469202573726	27.70956576201954	27.475691918154936	22.401273117251794
86-87	22.292500546759193	27.760890922408795	27.51016277802271	22.436445752809302
88-89	22.301572982711953	27.74875548697485	27.484304177412746	22.465367352900444
90-91	22.319013091397956	27.82483420234636	27.51099533227377	22.345157373981916
92-93	22.359471551143866	27.77615891564898	27.55679676758249	22.307572765624663
94-95	22.182293234543643	27.84528125702494	27.62482477740059	22.347600731030823
96-97	22.228184065111602	27.983805779397976	27.676940162733153	22.11106999275727
98-99	23.300374627056183	29.96287656029598	27.853459323849318	18.883289488798518
100	22.26819546369812	31.021808843974323	21.587627741410532	25.122367950917024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1858.0
1	1534.0
2	1820.0
3	2565.5
4	3289.5
5	5054.0
6	6745.0
7	8084.0
8	9077.0
9	9063.5
10	8204.5
11	7338.5
12	7859.0
13	10156.0
14	15441.5
15	22543.0
16	28435.5
17	32347.5
18	33923.0
19	33604.0
20	32535.0
21	31706.0
22	32652.0
23	36926.5
24	44235.5
25	54333.5
26	68261.0
27	84095.5
28	100581.0
29	119538.5
30	138027.5
31	154907.5
32	171864.0
33	187787.5
34	203564.0
35	219046.5
36	235647.0
37	252787.0
38	266287.0
39	278523.0
40	292570.0
41	305047.0
42	313581.0
43	321854.5
44	330878.5
45	339653.5
46	348638.5
47	355883.5
48	359998.0
49	357250.0
50	349865.0
51	340361.5
52	325875.5
53	307606.0
54	288544.0
55	268095.5
56	246837.0
57	225158.0
58	200740.5
59	174991.5
60	147995.5
61	121775.0
62	99069.0
63	78669.5
64	60482.0
65	46289.5
66	35450.5
67	27374.5
68	20913.5
69	15570.0
70	11444.5
71	8194.5
72	5842.5
73	4056.5
74	2750.5
75	1859.5
76	1271.5
77	832.5
78	563.5
79	377.5
80	241.0
81	152.5
82	111.0
83	74.5
84	46.0
85	33.0
86	26.0
87	20.5
88	14.0
89	8.5
90	7.0
91	7.0
92	5.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.004709248285450166
22-23	0.004589199458525173
24-25	0.00344253567921958
26-27	0.016977746721319326
28-29	0.021280794080169252
30-31	0.03923101363592462
32-33	0.022926382391007347
34-35	0.01271579944807665
36-37	0.006799843481138298
38-39	0.002672440351586513
40-41	0.007155444817570621
42-43	0.009220035993270632
44-45	0.005588595352124728
46-47	0.001912915278135507
48-49	0.009870345665270907
50-51	0.00427559989408173
52-53	2.1039138478136723E-5
54-55	4.607514870931458E-4
56-57	6.095038137012155E-4
58-59	3.7740428301605245E-4
60-61	9.036374271635173E-4
62-63	8.47508911110143E-4
64-65	0.0
66-67	2.2870244675787983E-4
68-69	5.254103918757955E-4
70-71	3.131639810376078E-4
72-73	7.212498514899002E-4
74-75	3.8499263420863284E-4
76-77	0.0
78-79	0.0
80-81	2.916415125862061E-4
82-83	0.002263332867435834
84-85	2.5675962506247814E-5
86-87	6.769353712443756E-4
88-89	2.466643926558848E-4
90-91	0.008812506666473222
92-93	7.382807501224088E-4
94-95	3.7187432655883676E-5
96-97	2.770227316256315E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	195.0
20-21	515.0
22-23	1191.0
24-25	2405.0
26-27	4603.0
28-29	10794.0
30-31	24128.0
32-33	30781.0
34-35	41915.0
36-37	64379.0
38-39	73318.0
40-41	72875.0
42-43	78185.0
44-45	90267.0
46-47	88090.0
48-49	82657.0
50-51	74859.0
52-53	74494.0
54-55	79644.0
56-57	83554.0
58-59	83587.0
60-61	81384.0
62-63	79891.0
64-65	84449.0
66-67	87505.0
68-69	86482.0
70-71	79622.0
72-73	74628.0
74-75	75752.0
76-77	75649.0
78-79	80461.0
80-81	80770.0
82-83	78273.0
84-85	79736.0
86-87	84202.0
88-89	90690.0
90-91	96483.0
92-93	102949.0
94-95	125673.0
96-97	286001.0
98-99	503998.0
100-101	4491985.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.05216092093295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.94211144619918	79.16707320103949
2	6.467718169336231	11.26057685736599
3	1.4723429651311517	3.845119101942017
4	0.5249282588612065	1.8278455704932357
5	0.2322478544726865	1.0108838800548863
6	0.1253250104158739	0.6545887784484151
7	0.07182930821883945	0.4377027548533991
8	0.03949843740963811	0.2750739463607372
9	0.03133383603539825	0.24549103231412545
>10	0.09166988350604546	1.1710667986259669
>50	7.690736930827645E-4	0.04359222419384692
>100	1.8238300436278993E-4	0.03702855731526205
>500	4.3373716277012156E-5	0.023957296992569026
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2675847275814648E-5	0.0	0.0	0.0
3	0.0	1.2675847275814648E-5	0.0	0.0	0.0
4	0.0	5.070338910325859E-5	0.0	0.0	0.0
5	0.0	7.605508365488788E-5	0.0	0.0	0.0
6	1.1408262548233183E-4	7.605508365488788E-5	0.0	0.0	0.0
7	1.1408262548233183E-4	7.605508365488788E-5	0.0	0.0	0.0
8	1.1408262548233183E-4	1.1408262548233183E-4	0.0	0.0	1.2675847275814648E-5
9	1.1408262548233183E-4	1.3943432003396113E-4	0.0	0.0	1.2675847275814648E-5
10-11	1.1408262548233183E-4	1.3943432003396113E-4	0.0	0.0	1.2675847275814648E-5
12-13	1.1408262548233183E-4	1.3943432003396113E-4	0.0	6.337923637907324E-6	1.2675847275814648E-5
14-15	1.1408262548233183E-4	2.53516945516293E-4	0.0	1.2675847275814648E-5	1.2675847275814648E-5
16-17	1.6478601458559043E-4	2.53516945516293E-4	0.0	1.2675847275814648E-5	1.2675847275814648E-5
18-19	1.6478601458559043E-4	2.661927927921076E-4	0.0	1.2675847275814648E-5	1.2675847275814648E-5
20-21	1.6478601458559043E-4	2.7886864006792226E-4	0.0	2.5351694551629296E-5	1.2675847275814648E-5
22-23	1.6478601458559043E-4	2.7886864006792226E-4	0.0	3.802754182744394E-5	1.901377091372197E-5
24-25	1.7112393822349775E-4	3.042203346195516E-4	0.0	5.070338910325859E-5	2.5351694551629296E-5
26-27	1.7746186186140507E-4	3.168961818953662E-4	0.0	1.0774470184442451E-4	2.5351694551629296E-5
28-29	1.7746186186140507E-4	3.168961818953662E-4	0.0	6.591440583423617E-4	2.5351694551629296E-5
30-31	1.7746186186140507E-4	3.612616473607175E-4	0.0	0.002287990433284544	2.5351694551629296E-5
32-33	1.7746186186140507E-4	3.866133419123468E-4	0.0	0.005089352681239581	2.5351694551629296E-5
34-35	1.7746186186140507E-4	4.6900634920514196E-4	0.0	0.00869563123120885	3.168961818953662E-5
36-37	1.7746186186140507E-4	5.640752037737518E-4	0.0	0.014799051694513603	3.802754182744394E-5
38-39	1.837997854993124E-4	5.767510510495665E-4	0.0	0.024362978464115753	3.802754182744394E-5
40-41	1.9013770913721973E-4	6.337923637907324E-4	0.0	0.03381916053187348	3.802754182744394E-5
42-43	1.9013770913721973E-4	6.654819819802691E-4	0.0	0.043040839425028635	3.802754182744394E-5
44-45	1.9647563277512705E-4	7.605508365488789E-4	0.0	0.05253504903461381	3.802754182744394E-5
46-47	2.0281355641303437E-4	8.239300729279522E-4	0.0	0.06302431265535044	3.802754182744394E-5
48-49	2.0281355641303437E-4	8.239300729279522E-4	0.0	0.07348822458153542	3.802754182744394E-5
50-51	2.1548940368884903E-4	9.506885456860986E-4	0.0	0.08309651681660293	3.802754182744394E-5
52-53	2.1548940368884903E-4	9.633643929619133E-4	0.0	0.0919062306732941	4.4365465465351267E-5
54-55	2.1548940368884903E-4	9.697023165998206E-4	0.0	0.10051946889721017	5.070338910325859E-5
56-57	2.2816525096466366E-4	9.887160875135426E-4	0.0	0.10850525268097339	5.070338910325859E-5
58-59	2.2816525096466366E-4	0.001014067782065172	0.0	0.11652906400656406	5.070338910325859E-5
60-61	2.34503174602571E-4	0.0010204057057030791	0.0	0.12440710308848288	5.070338910325859E-5
62-63	2.4084109824047833E-4	0.0010901228657200597	0.0	0.13127741231197443	5.070338910325859E-5
64-65	2.4084109824047833E-4	0.001191529643926577	0.0	0.1382554662373104	5.070338910325859E-5
66-67	2.4084109824047833E-4	0.001191529643926577	0.0	0.14549337503180054	5.7041312741165916E-5
68-69	2.4084109824047833E-4	0.0012232192621161135	0.0	0.15219889824070648	6.337923637907325E-5
70-71	2.4084109824047833E-4	0.001248570956667743	0.0	0.1579410570566505	6.337923637907325E-5
72-73	2.53516945516293E-4	0.00125490888030565	0.0	0.1624409828395647	6.337923637907325E-5
74-75	2.5985486915420034E-4	0.0012802605748572795	0.0	0.16625641286958492	6.337923637907325E-5
76-77	2.725307164300149E-4	0.0012992743457710015	0.0	0.17010987044143258	6.337923637907325E-5
78-79	2.7886864006792226E-4	0.0013056122694089089	0.0	0.17265137782023343	6.337923637907325E-5
80-81	2.7886864006792226E-4	0.0013056122694089089	0.0	0.17349432166407508	6.337923637907325E-5
82-83	2.7886864006792226E-4	0.0013056122694089089	0.0	0.17400769347874556	7.605508365488788E-5
84-85	2.7886864006792226E-4	0.001330963963960538	0.0	0.17415346572241747	7.605508365488788E-5
86-87	2.915444873437369E-4	0.0013563156585121674	0.0	0.1742041691115207	7.605508365488788E-5
88	3.0422033461955153E-4	0.0014323707421670553	0.0	0.17421684495879652	7.605508365488788E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7775	0.0	44.907875	1
GTATCAA	13240	0.0	33.142986	1
TCAACGC	15430	0.0	27.879244	4
ATCAACG	15450	0.0	27.710058	3
CAACGCA	15905	0.0	26.96906	5
TATCAAC	16410	0.0	26.28949	2
AACGCAG	16455	0.0	26.192598	6
CTTATAC	6385	0.0	25.828468	1
ACGCAGA	19300	0.0	22.28895	7
TATACAC	8745	0.0	21.867964	3
CGCAGAG	19725	0.0	21.746155	8
GTACATG	19245	0.0	20.237053	1
TACATGG	19085	0.0	20.04038	2
ACATGGG	19560	0.0	19.30141	3
TTATACA	9555	0.0	19.024218	2
GCAGAGT	24005	0.0	17.783237	9
CATGGGG	15110	0.0	17.4193	4
ACATCTC	10145	0.0	17.390835	8
GAGTACT	13710	0.0	17.128284	12-13
TACACAT	11265	0.0	16.86654	5
>>END_MODULE