FastQCFastQC Report
Fri 27 May 2016
SRR2140200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8948288
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC123950.051.8218771
GGTATCA84300.041.6757241
TATACAC163800.040.682473
TTATACA166000.039.3330732
GTATCAA145900.034.3600121
TACACAT223300.030.243865
TCAACGC171550.028.5810624
ATCAACG172650.028.3753593
ACACATC229800.028.2887746
CAACGCA175200.027.9623585
ACATCTC239600.027.2507938
AACGCAG181000.027.1789196
TATCAAC184600.026.8034322
ATACACA270550.026.0917874
CACATCT253800.025.5655367
CATCTCC276300.023.306649
ACGCAGA213650.022.930057
CGCAGAG219300.022.3207028
GTACATG225350.021.1607861
TACATGG223450.020.7023162
Produced by FastQC (version 0.11.5)