##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8948288
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.138548736920406	33.0	31.0	34.0	30.0	34.0
2	32.43197827338592	34.0	31.0	34.0	31.0	34.0
3	32.22520039587461	33.0	31.0	34.0	30.0	34.0
4	36.05417840820501	37.0	35.0	37.0	35.0	37.0
5	35.88790794395531	37.0	35.0	37.0	35.0	37.0
6	35.98570832767117	37.0	35.0	37.0	35.0	37.0
7	35.94841381949262	37.0	35.0	37.0	35.0	37.0
8	35.9230861814014	37.0	35.0	37.0	35.0	37.0
9	37.7105517837602	39.0	37.0	39.0	35.0	39.0
10-11	37.63300236872126	39.0	37.0	39.0	35.0	39.0
12-13	37.60168950753485	39.0	37.0	39.0	35.0	39.0
14-15	38.91822268125478	40.0	38.0	41.0	35.0	41.0
16-17	38.88941068950843	40.0	38.0	41.0	35.0	41.0
18-19	38.85515659531745	40.0	38.0	41.0	35.0	41.0
20-21	38.842668733671005	40.0	38.0	41.0	35.0	41.0
22-23	38.74752995472559	40.0	38.0	41.0	34.0	41.0
24-25	38.67914056474283	40.0	38.0	41.0	34.0	41.0
26-27	38.51847819090739	40.0	38.0	41.0	34.0	41.0
28-29	38.38374504035774	40.0	38.0	41.0	34.0	41.0
30-31	38.23164774253931	40.0	38.0	41.0	34.0	41.0
32-33	38.08081436826885	40.0	37.5	41.0	33.0	41.0
34-35	38.19044526724062	40.0	38.0	41.0	33.5	41.0
36-37	38.17640384520105	40.0	38.0	41.0	33.0	41.0
38-39	38.09437460944824	40.0	38.0	41.0	33.0	41.0
40-41	38.095344214128204	40.0	37.5	41.0	33.0	41.0
42-43	38.18578438778416	40.0	38.0	41.0	33.0	41.0
44-45	38.15164621660511	40.0	38.0	41.0	33.0	41.0
46-47	38.07182043999993	40.0	37.0	41.0	33.0	41.0
48-49	37.976628957867504	40.0	37.0	41.0	33.0	41.0
50-51	37.83521255185227	40.0	37.0	41.0	33.0	41.0
52-53	37.693256560638844	40.0	37.0	41.0	32.5	41.0
54-55	37.52483982861142	39.0	36.0	41.0	32.0	41.0
56-57	37.396789539538496	39.0	36.0	41.0	32.0	41.0
58-59	37.19367663479607	39.0	35.5	41.0	31.0	41.0
60-61	36.98662158431047	39.0	35.0	40.5	31.0	41.0
62-63	36.73172192872373	38.0	35.0	40.0	31.0	41.0
64-65	36.44337664088149	38.0	35.0	40.0	31.0	41.0
66-67	36.16294668812925	37.0	35.0	40.0	30.0	41.0
68-69	35.89489551947497	37.0	34.0	39.5	30.0	41.0
70-71	35.59452807138585	36.5	34.0	39.0	30.0	41.0
72-73	35.1354804801709	36.0	34.0	39.0	29.0	40.0
74-75	34.894938987164366	35.5	34.0	38.5	29.0	40.0
76-77	33.668612315626156	34.5	32.0	36.5	28.0	39.0
78-79	34.38781824869767	35.0	33.5	37.0	29.5	39.0
80-81	34.30578026137981	35.0	34.0	36.5	30.0	39.0
82-83	34.06748391700106	35.0	34.0	36.0	30.0	37.5
84-85	33.846516217958936	35.0	34.0	36.0	29.0	37.0
86-87	33.656007942249296	35.0	33.0	35.5	29.0	37.0
88-89	33.479571127739284	35.0	33.0	35.0	29.0	36.0
90-91	33.32776128758367	35.0	33.0	35.0	29.0	36.0
92-93	33.24255736751106	35.0	33.0	35.0	29.0	36.0
94-95	33.2076378216835	35.0	33.0	35.0	29.0	36.0
96-97	33.25830087345519	35.0	33.0	35.0	29.0	35.0
98-99	33.35296621101902	35.0	33.0	35.0	29.5	35.0
100	32.18257179150968	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	9.0
20	44.0
21	286.0
22	1291.0
23	4368.0
24	11886.0
25	24960.0
26	45301.0
27	71631.0
28	103597.0
29	137243.0
30	176261.0
31	218658.0
32	271830.0
33	346870.0
34	463290.0
35	648977.0
36	1020305.0
37	1792645.0
38	2310019.0
39	1248120.0
40	50697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96896523670226	20.271274237038416	13.826376620868707	24.93338390539062
2	14.909645286338572	20.747398832044745	40.747749737156425	23.59520614446026
3	18.216680106853957	25.409609078295198	30.867781635995623	25.50592917885522
4	12.584518960498366	17.932167583341084	37.363001727257775	32.12031172890278
5	13.255082983471253	37.44354227311415	35.767992715478094	13.533382027936517
6	30.79827113298097	37.24258763240522	18.106055594098	13.853085640515816
7	26.854656443780083	32.19423648411853	22.10187021249204	18.84923685960935
8	24.921638641939108	36.44682647675175	21.004151855639872	17.627383025669268
9	25.010169543045553	16.510934829098034	20.57256091891544	37.906334708940975
10-11	24.35530684752212	26.40930309797807	28.548019464728895	20.687370589770914
12-13	24.968977306050053	23.622730962615417	28.584730397591134	22.823561333743392
14-15	22.14787342562063	25.39748385389473	25.57454006844661	26.880102652038023
16-17	22.328338113391073	28.887413994721673	26.33093615225616	22.45331173963109
18-19	22.49790686218414	27.62691589720849	27.743820940944236	22.131356299663132
20-21	22.899528072745117	26.950303602938913	27.644092717044433	22.50607560727154
22-23	22.45769280938434	27.344624192728297	27.604850174596134	22.59283282329123
24-25	22.596113804656365	27.26596070428696	27.735829671996132	22.402095819060534
26-27	22.658179478155795	27.495774030842824	27.76682772334192	22.079218767659466
28-29	22.256028370898026	27.12543458552206	27.91238829750826	22.70614874607165
30-31	22.42722523329624	27.12973453475224	28.094894478074316	22.348145753877205
32-33	21.928415912934643	26.91538229799753	28.52572445907573	22.630477329992097
34-35	22.01520034664128	27.14956450704149	28.101060256031996	22.73417489028523
36-37	22.20351184743306	27.26658775879286	28.191768182699533	22.338132211074544
38-39	22.19960043224403	27.031182909736096	28.143007420953513	22.62620923706636
40-41	22.30958959858991	26.867417625486084	28.32325783927762	22.499734936646387
42-43	22.527955545752146	26.824948252111913	28.3417215795632	22.305374622572742
44-45	22.149564081674313	27.297182341461117	28.085800449979065	22.46745312688551
46-47	22.801091685356827	26.785305626225703	27.42438586720379	22.989216821213684
48-49	22.48845824793802	26.871758996045124	27.714615124295932	22.92516763172092
50-51	22.244500696572725	27.18036193400102	27.90001306532614	22.675124304100112
52-53	22.31339689425321	27.407239903177246	28.152729633759094	22.12663356881045
54-55	22.240349109223278	27.441673663925513	27.605140543841184	22.712836683010025
56-57	22.66431672014788	27.85930565217818	27.002986153095154	22.473391474578786
58-59	22.031876643898734	28.23059208877754	27.783928411119756	21.95360285620397
60-61	22.581823244781326	28.584426053037426	26.876613160879053	21.957137541302192
62-63	22.15614121998745	28.920116252187984	26.8577033587635	22.066039169061067
64-65	22.218243573612796	28.58361130052711	26.96954335922837	22.228601766631723
66-67	22.33262316987031	28.28284274248797	27.004228124300013	22.380305963341716
68-69	22.418271480643	27.94298886117123	27.219164977069564	22.419574681116202
70-71	22.471376034151643	27.50772973794921	27.332029556039895	22.68886467185925
72-73	22.545966607119976	27.570601195778217	27.258810045004804	22.624622152097004
74-75	22.408909242147846	27.68433683200081	27.278635754252438	22.628118171598903
76-77	22.541563154933844	27.598823528895906	27.231450358873964	22.628162957296286
78-79	22.480424007394	27.575552874890207	27.230319534240362	22.71370358347544
80-81	22.43936070460802	27.62845067395671	27.25808063708629	22.674107984348982
82-83	22.50753193398522	27.607460992394206	27.374440001868244	22.51056707175233
84-85	22.615475447772955	27.567360613619872	27.252333655805312	22.56483028280186
86-87	22.434789530605663	27.63645735598398	27.329372053876927	22.59938105953343
88-89	22.498351313399404	27.597139915810192	27.292357192299026	22.61215157849138
90-91	22.50885350305098	27.64331999217652	27.318857093389337	22.528969411383166
92-93	22.54155662320332	27.594252128744547	27.39679656898668	22.46739467906546
94-95	22.31889041627841	27.70716839595304	27.416839511432915	22.55710167633563
96-97	22.399613378617357	27.811976468174006	27.472682578154167	22.31572757505447
98-99	23.496100472815364	29.808794598591497	27.677079570380535	19.0180253582126
100	30.14359918072616	41.2755255408942	28.58087527837964	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1675.0
1	1444.5
2	1740.5
3	2431.0
4	3204.0
5	5078.0
6	6830.5
7	8021.5
8	8870.5
9	8815.0
10	8061.5
11	7425.0
12	8040.5
13	10375.0
14	16099.5
15	24052.0
16	30741.0
17	35177.0
18	36943.5
19	36490.0
20	35261.5
21	34183.5
22	34573.0
23	38092.5
24	45059.0
25	55735.5
26	70067.5
27	86421.5
28	104116.5
29	124053.0
30	143152.0
31	161807.5
32	183052.0
33	203905.0
34	223734.0
35	243276.5
36	263944.5
37	283417.0
38	299688.5
39	314898.0
40	330249.0
41	344102.0
42	354778.5
43	364686.0
44	376308.0
45	388907.0
46	409923.0
47	433717.0
48	450919.5
49	449312.5
50	431856.5
51	413101.0
52	389642.5
53	363193.5
54	334822.0
55	307539.0
56	281479.0
57	255376.0
58	227976.0
59	197277.0
60	166516.5
61	137944.5
62	111406.5
63	87965.0
64	67581.0
65	52127.5
66	40342.0
67	31118.5
68	23767.5
69	17862.0
70	13283.0
71	9531.5
72	6842.5
73	4880.5
74	3358.0
75	2274.5
76	1547.5
77	1010.0
78	686.0
79	457.0
80	283.5
81	185.0
82	142.0
83	101.5
84	65.5
85	40.0
86	23.5
87	15.5
88	12.0
89	11.0
90	9.0
91	7.0
92	6.0
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005023520020152194
22-23	0.0048395677695963375
24-25	0.0032810377648564634
26-27	0.015656017611230554
28-29	0.020697659401913183
30-31	0.03863318785908909
32-33	0.023185368171331266
34-35	0.012045372597596176
36-37	0.006756418498130563
38-39	0.0027630751352359035
40-41	0.007644583465396744
42-43	0.008606134555540768
44-45	0.005994872405490098
46-47	0.002026602535955307
48-49	0.009490940204959546
50-51	0.004330454274439726
52-53	6.18012289545185E-6
54-55	4.873429841822837E-4
56-57	6.706856767670875E-4
58-59	3.785978020793361E-4
60-61	0.0010810185643448298
62-63	9.775656607075673E-4
64-65	0.0
66-67	1.7041712727575883E-4
68-69	6.100050110525283E-4
70-71	2.745527799208148E-4
72-73	6.203980017194295E-4
74-75	4.1139396731886327E-4
76-77	0.0
78-79	7.402880134933776E-6
80-81	3.22660160437137E-4
82-83	0.0018940726555621268
84-85	6.167773936282578E-5
86-87	5.864550465786056E-4
88-89	2.9376944386506584E-4
90-91	0.008308932069675846
92-93	8.220054812969504E-4
94-95	1.677425674945769E-5
96-97	1.6382235138704505E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	252.0
20-21	682.0
22-23	1461.0
24-25	2690.0
26-27	5012.0
28-29	12114.0
30-31	26898.0
32-33	34639.0
34-35	48471.0
36-37	72973.0
38-39	82329.0
40-41	82031.0
42-43	88336.0
44-45	101334.0
46-47	99473.0
48-49	93358.0
50-51	85058.0
52-53	85482.0
54-55	96636.0
56-57	108292.0
58-59	114205.0
60-61	110634.0
62-63	110105.0
64-65	119652.0
66-67	121444.0
68-69	117058.0
70-71	95107.0
72-73	83872.0
74-75	85721.0
76-77	86240.0
78-79	90549.0
80-81	91315.0
82-83	87477.0
84-85	89487.0
86-87	95199.0
88-89	103552.0
90-91	108427.0
92-93	115913.0
94-95	141593.0
96-97	322379.0
98-99	1818282.0
100-101	3712556.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.66237011782076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.78406120827349	78.67561913230291
2	6.527159075147402	11.313181511766338
3	1.5398436308448455	4.003394959795349
4	0.5346333515190341	1.85330373546694
5	0.2390882918117286	1.0359979017912782
6	0.12911556507185423	0.6713676532937162
7	0.0722865133032657	0.438516439929007
8	0.04704511242576793	0.3261632756221111
9	0.028116706998937896	0.219299442159265
>10	0.09654825995931993	1.2737160047858884
>50	0.0017159548418654049	0.09965603451557618
>100	3.466742163297228E-4	0.05101852999930789
>500	1.9827794647322623E-5	0.015447729868334304
>1k	1.9827791576640656E-5	0.023317648704013226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3525966084238684E-5
2	0.0	1.117532202807956E-5	0.0	2.235064405615912E-5	3.3525966084238684E-5
3	0.0	1.117532202807956E-5	0.0	3.3525966084238684E-5	3.3525966084238684E-5
4	0.0	3.3525966084238684E-5	0.0	4.470128811231824E-5	3.3525966084238684E-5
5	1.117532202807956E-5	4.470128811231824E-5	0.0	4.470128811231824E-5	3.3525966084238684E-5
6	1.5645450839311387E-4	4.470128811231824E-5	0.0	4.470128811231824E-5	4.470128811231824E-5
7	1.5645450839311387E-4	4.470128811231824E-5	0.0	6.705193216847737E-5	4.470128811231824E-5
8	1.5645450839311387E-4	4.470128811231824E-5	0.0	7.822725419655693E-5	4.470128811231824E-5
9	1.5645450839311387E-4	1.1175322028079561E-4	0.0	1.2292854230887517E-4	8.940257622463648E-5
10-11	1.5645450839311387E-4	1.1175322028079561E-4	0.0	1.5086684737907407E-4	8.940257622463648E-5
12-13	1.5645450839311387E-4	1.1175322028079561E-4	0.0	1.6762983042119343E-4	8.940257622463648E-5
14-15	1.5645450839311387E-4	1.9556813549139232E-4	0.0	1.7880515244927297E-4	1.0057789825271605E-4
16-17	2.682077286739095E-4	2.011557965054321E-4	0.0	1.9556813549139232E-4	1.0057789825271605E-4
18-19	2.79383050701989E-4	2.1233111853351166E-4	0.0	2.570324066458299E-4	1.0057789825271605E-4
20-21	2.79383050701989E-4	2.3468176258967078E-4	0.0	2.9614603374410837E-4	1.0057789825271605E-4
22-23	2.905583727300686E-4	2.514447456317901E-4	0.0	3.2408433881430727E-4	1.0616555926675583E-4
24-25	3.240843388143073E-4	2.9614603374410837E-4	0.0	3.5202264388450616E-4	1.1175322028079561E-4
26-27	3.464349828704664E-4	3.2408433881430727E-4	0.0	4.58188203151262E-4	1.3410386433695474E-4
28-29	3.5761030489854593E-4	3.2408433881430727E-4	0.0	9.94603660499081E-4	1.3410386433695474E-4
30-31	3.7996094895470506E-4	3.464349828704664E-4	0.0	0.002391518914009026	1.3410386433695474E-4
32-33	3.7996094895470506E-4	3.5202264388450616E-4	0.0	0.005626774641138059	1.8998047447735253E-4
34-35	3.7996094895470506E-4	3.967239319968244E-4	0.0	0.009616364605162462	2.1233111853351166E-4
36-37	3.7996094895470506E-4	4.4701288112318244E-4	0.0	0.015477821008890193	2.2350644056159122E-4
38-39	3.7996094895470506E-4	4.5818820315126203E-4	0.0	0.024367789682227484	2.2350644056159122E-4
40-41	3.7996094895470506E-4	5.36415457347819E-4	0.0	0.033805349134940675	2.2350644056159122E-4
42-43	3.7996094895470506E-4	5.587661014039781E-4	0.0	0.04298028851999399	2.3468176258967078E-4
44-45	3.967239319968244E-4	6.314056945864952E-4	0.0	0.052870448514844404	2.3468176258967078E-4
46-47	4.134869150389438E-4	6.928699657409328E-4	0.0	0.06310145583155125	2.3468176258967078E-4
48-49	4.134869150389438E-4	6.928699657409328E-4	0.0	0.07316483331783688	2.3468176258967078E-4
50-51	4.6377586416530175E-4	8.102108470357681E-4	0.0	0.08299352904153286	2.4585708461775035E-4
52-53	4.805388472074211E-4	8.157985080498079E-4	0.0	0.09263783195176552	2.4585708461775035E-4
54-55	4.8612650822146093E-4	8.213861690638477E-4	0.0	0.10113107669310599	2.4585708461775035E-4
56-57	4.917141692355007E-4	8.269738300778876E-4	0.0	0.10935611370577256	2.4585708461775035E-4
58-59	4.917141692355007E-4	8.269738300778876E-4	0.0	0.11737440726091963	2.4585708461775035E-4
60-61	5.531784403899384E-4	8.269738300778876E-4	0.0	0.12473894447742406	2.4585708461775035E-4
62-63	6.034673895162963E-4	8.549121351480864E-4	0.0	0.1311368163384996	2.4585708461775035E-4
64-65	6.034673895162963E-4	9.610776944148422E-4	0.0	0.13808227897895106	2.4585708461775035E-4
66-67	6.202303725584156E-4	9.778406774569616E-4	0.0	0.14537417660227298	2.4585708461775035E-4
68-69	6.481686776286145E-4	0.0010113666435412002	0.0	0.15243139246300522	2.514447456317901E-4
70-71	6.761069826988135E-4	0.00101695430455524	0.0	0.1587510370698842	2.682077286739095E-4
72-73	7.934478639936488E-4	0.0010225419655692798	0.0	0.16354525021993033	2.682077286739095E-4
74-75	7.934478639936488E-4	0.001044892609625439	0.0	0.16726663245528084	2.682077286739095E-4
76-77	7.990355250076886E-4	0.0010895938977377572	0.0	0.1711947581481508	2.682077286739095E-4
78-79	8.046231860217284E-4	0.0011119445417939163	0.0	0.17329013102841573	2.682077286739095E-4
80-81	8.046231860217284E-4	0.0011342951858500754	0.0	0.17402211462125494	2.682077286739095E-4
82-83	8.046231860217284E-4	0.0011510581688921949	0.0	0.17461999434975717	2.7379538968794924E-4
84-85	8.102108470357681E-4	0.001173408812948354	0.0	0.17480997482423455	2.79383050701989E-4
86-87	8.269738300778876E-4	0.0012013471180185528	0.0	0.1749049650614732	2.79383050701989E-4
88	8.381491521059671E-4	0.0012404607451168314	0.0	0.17491614038350128	2.79383050701989E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12395	0.0	51.821877	1
GGTATCA	8430	0.0	41.675724	1
TATACAC	16380	0.0	40.68247	3
TTATACA	16600	0.0	39.333073	2
GTATCAA	14590	0.0	34.360012	1
TACACAT	22330	0.0	30.24386	5
TCAACGC	17155	0.0	28.581062	4
ATCAACG	17265	0.0	28.375359	3
ACACATC	22980	0.0	28.288774	6
CAACGCA	17520	0.0	27.962358	5
ACATCTC	23960	0.0	27.250793	8
AACGCAG	18100	0.0	27.178919	6
TATCAAC	18460	0.0	26.803432	2
ATACACA	27055	0.0	26.091787	4
CACATCT	25380	0.0	25.565536	7
CATCTCC	27630	0.0	23.30664	9
ACGCAGA	21365	0.0	22.93005	7
CGCAGAG	21930	0.0	22.320702	8
GTACATG	22535	0.0	21.160786	1
TACATGG	22345	0.0	20.702316	2
>>END_MODULE