FastQCFastQC Report
Fri 27 May 2016
SRR2140199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9596305
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89550.042.798041
GTATCAA157450.034.4317361
CTTATAC88150.033.1657261
TCAACGC180050.029.063734
ATCAACG181500.028.831543
CAACGCA184300.028.349085
AACGCAG190000.027.5201576
TATACAC119700.027.4685043
TATCAAC194600.027.2904552
TTATACA130700.023.6529312
ACGCAGA227450.022.9349147
CGCAGAG237950.021.9228658
TACACAT154400.020.9504535
GTACATG244950.020.7447381
ACATCTC145100.020.6565258
TACATGG245800.020.2232272
ACACATC149700.020.1038466
ACATGGG246700.019.850693
GCAGAGT285650.018.0326719
CACATCT169600.017.5759647
Produced by FastQC (version 0.11.5)