##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9596305
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16125758820713	33.0	31.0	34.0	31.0	34.0
2	32.45193780314402	34.0	31.0	34.0	31.0	34.0
3	32.246425994171716	33.0	31.0	34.0	30.0	34.0
4	36.06812299108876	37.0	35.0	37.0	35.0	37.0
5	35.906019556485546	37.0	35.0	37.0	35.0	37.0
6	36.0024347913077	37.0	35.0	37.0	35.0	37.0
7	35.964997048343086	37.0	35.0	37.0	35.0	37.0
8	35.93984340847857	37.0	35.0	37.0	35.0	37.0
9	37.72756243158174	39.0	37.0	39.0	35.0	39.0
10-11	37.650386216361404	39.0	37.0	39.0	35.0	39.0
12-13	37.62268367877011	39.0	37.0	39.0	35.0	39.0
14-15	38.950256687339554	40.0	38.0	41.0	35.5	41.0
16-17	38.928094407170256	40.0	38.0	41.0	35.0	41.0
18-19	38.893864982407294	40.0	38.0	41.0	35.0	41.0
20-21	38.882219163880094	40.0	38.0	41.0	35.0	41.0
22-23	38.79248133372023	40.0	38.0	41.0	34.5	41.0
24-25	38.728664588344415	40.0	38.0	41.0	34.0	41.0
26-27	38.580781569427046	40.0	38.0	41.0	34.0	41.0
28-29	38.4553646644535	40.0	38.0	41.0	34.0	41.0
30-31	38.306901483943555	40.0	38.0	41.0	34.0	41.0
32-33	38.15337232837432	40.0	38.0	41.0	33.5	41.0
34-35	38.25458790151751	40.0	38.0	41.0	34.0	41.0
36-37	38.24799827137244	40.0	38.0	41.0	34.0	41.0
38-39	38.17791947554021	40.0	38.0	41.0	33.0	41.0
40-41	38.16498931396018	40.0	38.0	41.0	33.0	41.0
42-43	38.23864363741506	40.0	38.0	41.0	33.0	41.0
44-45	38.199768497791396	40.0	38.0	41.0	33.0	41.0
46-47	38.119552107033755	40.0	37.5	41.0	33.0	41.0
48-49	38.02496299022752	40.0	37.0	41.0	33.0	41.0
50-51	37.90459039507121	40.0	37.0	41.0	33.0	41.0
52-53	37.755146959453185	40.0	37.0	41.0	33.0	41.0
54-55	37.591846699359195	39.5	36.0	41.0	32.0	41.0
56-57	37.47596278135536	39.0	36.0	41.0	32.0	41.0
58-59	37.27056796256207	39.0	35.5	41.0	32.0	41.0
60-61	37.06367354825878	39.0	35.0	41.0	31.0	41.0
62-63	36.800803643015485	38.5	35.0	40.0	31.0	41.0
64-65	36.4957091609555	38.0	35.0	40.0	31.0	41.0
66-67	36.20993650235839	37.0	35.0	40.0	31.0	41.0
68-69	35.92957790908635	37.0	34.5	39.5	30.0	41.0
70-71	35.62055721210301	36.5	34.0	39.0	30.0	41.0
72-73	35.157674802401985	36.0	34.0	39.0	29.0	40.0
74-75	34.91434848678895	35.5	34.0	38.5	29.0	40.0
76-77	33.695137940878354	34.5	32.5	36.5	28.5	39.0
78-79	34.40078851143349	35.0	34.0	37.0	29.5	39.0
80-81	34.31629409404712	35.0	34.0	36.5	30.0	39.0
82-83	34.07432167104956	35.0	34.0	36.0	30.0	37.5
84-85	33.85754387130257	35.0	34.0	36.0	29.0	37.0
86-87	33.67034095466458	35.0	33.5	35.0	29.0	37.0
88-89	33.49703607690385	35.0	33.0	35.0	29.0	36.0
90-91	33.34819574022016	35.0	33.0	35.0	29.0	36.0
92-93	33.26743710181753	35.0	33.0	35.0	29.0	36.0
94-95	33.23068850893606	35.0	33.0	35.0	29.0	35.5
96-97	33.282545084858384	35.0	33.0	35.0	29.0	35.0
98-99	33.37818332028962	35.0	33.0	35.0	30.0	35.0
100	32.21908033407426	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	11.0
20	63.0
21	289.0
22	1352.0
23	4496.0
24	12008.0
25	25816.0
26	47180.0
27	74793.0
28	107519.0
29	144497.0
30	184689.0
31	230334.0
32	287071.0
33	365324.0
34	490562.0
35	687923.0
36	1083912.0
37	1920405.0
38	2479254.0
39	1389679.0
40	59125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56670718573451	20.160957785314242	13.75975440547169	24.512580623479558
2	15.145110539942197	20.879557287935306	40.532486201720346	23.44284597040215
3	18.49427461924147	25.343765126264746	30.383767502179225	25.77819275231456
4	12.799884955719937	17.61468606927354	37.834093434921044	31.751335540085478
5	13.48661802641746	37.46949476908039	35.56130198029346	13.482585224208693
6	31.06604052288876	37.20017235800655	18.20249564806454	13.531291471040156
7	27.3405440948365	32.067248800449754	22.353499602190634	18.23870750252311
8	24.97100706990868	36.533801291226155	20.845283679499556	17.64990795936561
9	25.408956884967704	16.641353104137476	20.83464416772914	37.11504584316568
10-11	24.65610461526598	26.501419035764286	28.43606992483044	20.40640642413929
12-13	25.170995502956607	23.76345374599911	28.63484955928349	22.43070119176079
14-15	22.260599261903412	25.37250535492567	25.91539139283297	26.451503990337947
16-17	22.265325039168722	28.6651580999145	26.652920056209133	22.416596804707645
18-19	22.35054534010747	27.81716504425401	28.04308533336529	21.78920428227323
20-21	22.96451623711382	26.99461825939999	27.959091038649724	22.081774464836464
22-23	22.638486328301592	27.094774054704303	27.831110993916248	22.435628623077854
24-25	22.587538462300234	27.13047720485698	27.943398296517852	22.338586036324934
26-27	22.57731588364135	27.3093575540256	27.885587859673322	22.227738702659728
28-29	22.412010245286424	27.251802268956983	27.8993890787952	22.43679840696139
30-31	22.65579780504126	27.166345053520796	27.913344001833785	22.264513139604155
32-33	22.25477104250988	27.154950017899637	28.097300600511048	22.492978339079432
34-35	22.37899781716555	27.250422696422977	27.902555809935215	22.468023676476257
36-37	22.44508608779741	27.249495773440202	27.978067919534826	22.327350219227558
38-39	22.330915529083164	27.25340526719241	27.99863611316412	22.417043090560302
40-41	22.57992126997779	27.10130358044634	27.938709337757988	22.38006581181788
42-43	22.594037643940844	27.110818984436065	27.85228509679109	22.442858274832005
44-45	22.435930632424352	27.385824359747062	27.71780940554513	22.460435602283457
46-47	22.74171653810612	27.135434777614382	27.364069054443753	22.75877962983574
48-49	22.597158461730416	27.21547499513702	27.444530276689637	22.74283626644293
50-51	22.432579694882474	27.364835428567712	27.57957705246608	22.623007824083736
52-53	22.590719675872915	27.39895993544395	27.587621253035866	22.422699135647267
54-55	22.54264729476635	27.35216848730134	27.377752813795116	22.72743140413719
56-57	22.719762533772013	27.583834679495844	27.135335981545083	22.56106680518706
58-59	22.425421761303056	27.783119371643828	27.48240687999413	22.309051987058982
60-61	22.68530491998888	27.901475142638088	27.084585844504826	22.328634092868207
62-63	22.52800488523949	28.09637937715646	27.04165852215137	22.333957215452678
64-65	22.505576269046205	27.93518528395868	27.09765956713118	22.461578879863932
66-67	22.57569222253574	27.83050058511762	27.022976014673944	22.570831177672698
68-69	22.59972491175214	27.66981066904312	27.177450800850234	22.55301361835451
70-71	22.604667455381268	27.392415611374982	27.22527080911718	22.77764612412657
72-73	22.66921812198715	27.43153082931009	27.154287006237627	22.744964042465135
74-75	22.50544389783947	27.55788622241915	27.222047990091507	22.714621889649873
76-77	22.648954819189974	27.476464461650885	27.151678495991554	22.722902223167587
78-79	22.579340525441868	27.513643292993883	27.088807536075	22.81820864548925
80-81	22.582116464429784	27.53457708574169	27.155708189130745	22.727598260697782
82-83	22.616686707956756	27.514434201118398	27.276524242134563	22.592354848790283
84-85	22.713264276663388	27.499782720749877	27.160423344270136	22.626529658316603
86-87	22.528240144973932	27.50390252537647	27.248190843261593	22.719666486388004
88-89	22.565302522516475	27.49699075121063	27.249721690081664	22.687985036191233
90-91	22.575493102599157	27.534626856746414	27.253897934953063	22.63598210570136
92-93	22.66045760758391	27.495877791869443	27.239146526005463	22.604518074541183
94-95	22.454594456514744	27.623785226645314	27.32486130498724	22.596759011852697
96-97	22.52082072584438	27.684156348363665	27.39213660268908	22.402886323102873
98-99	23.624047329086633	29.69810620174688	27.572140813337153	19.10570565582933
100	30.228089760647524	41.27261217602351	28.499298063328965	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1500.0
1	1329.5
2	1747.5
3	2476.5
4	3246.5
5	5186.5
6	6938.0
7	8152.5
8	9008.5
9	8973.5
10	8272.0
11	7515.5
12	7983.5
13	10223.0
14	15990.5
15	24098.0
16	31094.0
17	35879.5
18	37986.0
19	37741.5
20	36343.5
21	35045.0
22	35420.5
23	39167.5
24	46275.5
25	57326.5
26	72609.5
27	90657.0
28	110448.0
29	131898.0
30	152577.5
31	173206.5
32	195656.5
33	218141.0
34	239975.5
35	259764.5
36	281694.0
37	303785.5
38	321482.0
39	337878.0
40	356557.5
41	375221.5
42	387572.5
43	398392.5
44	410632.5
45	422507.5
46	437518.5
47	451889.5
48	460504.5
49	458813.0
50	446771.5
51	431177.0
52	409689.0
53	384508.0
54	358944.5
55	331532.0
56	304818.5
57	278407.5
58	249461.0
59	215565.0
60	181278.5
61	150402.0
62	122121.5
63	97122.5
64	75148.0
65	58177.0
66	45425.0
67	34724.0
68	26128.0
69	19803.5
70	14678.5
71	10457.5
72	7452.0
73	5188.0
74	3604.5
75	2486.5
76	1669.5
77	1094.5
78	744.5
79	515.0
80	340.5
81	226.5
82	167.0
83	115.5
84	75.0
85	53.0
86	40.5
87	29.0
88	16.5
89	9.5
90	8.5
91	7.0
92	6.0
93	4.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.004965622055406963
22-23	0.004841002005081541
24-25	0.003570185149280647
26-27	0.01701238530847318
28-29	0.021892285468515515
30-31	0.040583849237768414
32-33	0.024349742843854914
34-35	0.01265769998850066
36-37	0.007136121760991416
38-39	0.0028008178601954662
40-41	0.007868173448864887
42-43	0.009230893304169378
44-45	0.006070781789990394
46-47	0.002124477070563586
48-49	0.010331532152111751
50-51	0.00466733405359389
52-53	5.765579937488429E-6
54-55	4.6039919990778033E-4
56-57	6.074384315444311E-4
58-59	4.3595178516582685E-4
60-61	9.556291699151003E-4
62-63	8.756808188730159E-4
64-65	0.0
66-67	1.635018884782546E-4
68-69	6.059737788129369E-4
70-71	2.845780431999818E-4
72-73	5.436123841974631E-4
74-75	2.983039629880352E-4
76-77	0.0
78-79	1.3599094082748585E-5
80-81	2.82608584766515E-4
82-83	0.0020683748834442804
84-85	1.4166534268598481E-5
86-87	7.184273452988844E-4
88-89	2.626246591715534E-4
90-91	0.008739092177466481
92-93	8.005323389009855E-4
94-95	5.394066619188606E-5
96-97	2.6142440493467873E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	208.0
20-21	693.0
22-23	1478.0
24-25	2865.0
26-27	5356.0
28-29	12209.0
30-31	29111.0
32-33	37010.0
34-35	51207.0
36-37	78923.0
38-39	89684.0
40-41	89227.0
42-43	95352.0
44-45	108938.0
46-47	107373.0
48-49	100327.0
50-51	91922.0
52-53	90729.0
54-55	99294.0
56-57	105309.0
58-59	105871.0
60-61	103291.0
62-63	102355.0
64-65	108315.0
66-67	112040.0
68-69	110802.0
70-71	99381.0
72-73	91502.0
74-75	93264.0
76-77	94186.0
78-79	99400.0
80-81	99299.0
82-83	96294.0
84-85	97800.0
86-87	104084.0
88-89	111840.0
90-91	118308.0
92-93	126068.0
94-95	154768.0
96-97	349938.0
98-99	1985732.0
100-101	4034552.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.58112350119555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.46236129554411	77.41870514243733
2	6.575838949074175	11.25535370449378
3	1.5868508257215066	4.074134294821391
4	0.6010783454514392	2.0576384046389564
5	0.2836885092781884	1.2139190674203353
6	0.15787509659326332	0.8106676883586749
7	0.10000003741850572	0.599068088670612
8	0.05929102500610536	0.4059354026847982
9	0.043209959875223275	0.3328161221306869
>10	0.1276129924369591	1.6651114495457617
>50	0.0018932563944238066	0.10316026884178928
>100	2.599044135615801E-4	0.03712530137636926
>500	3.9802792489200805E-5	0.026365064579456608
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.084135508406621E-5	0.0	0.0	0.0
3	0.0	2.084135508406621E-5	0.0	1.0420677542033106E-5	0.0
4	0.0	1.1462745296236416E-4	0.0	2.084135508406621E-5	0.0
5	0.0	1.1462745296236416E-4	0.0	3.126203262609932E-5	0.0
6	2.3967558346676144E-4	1.1462745296236416E-4	0.0	3.126203262609932E-5	0.0
7	2.3967558346676144E-4	1.1462745296236416E-4	0.0	5.2103387710165526E-5	2.084135508406621E-5
8	2.3967558346676144E-4	1.4588948558846347E-4	0.0	5.2103387710165526E-5	3.126203262609932E-5
9	2.3967558346676144E-4	2.6051693855082763E-4	0.0	6.252406525219864E-5	4.168271016813242E-5
10-11	2.44885922237778E-4	2.6051693855082763E-4	0.0	6.773440402321518E-5	4.168271016813242E-5
12-13	2.5009626100879455E-4	2.709376160928607E-4	0.0	8.336542033626484E-5	4.168271016813242E-5
14-15	2.7093761609286077E-4	3.0219964871896007E-4	0.0	9.899643664931451E-5	4.689304893914897E-5
16-17	4.6372015062047316E-4	3.0219964871896007E-4	0.0	1.1462745296236416E-4	5.2103387710165526E-5
18-19	4.6893048939148973E-4	3.178306650320097E-4	0.0	1.1462745296236416E-4	6.773440402321518E-5
20-21	4.6893048939148973E-4	3.334616813450594E-4	0.0	1.1462745296236416E-4	8.336542033626484E-5
22-23	4.6893048939148973E-4	3.9077540782624147E-4	0.0	1.3025846927541384E-4	8.85757591072814E-5
24-25	4.741408281625063E-4	4.5850981184945665E-4	0.0	1.5631016313049658E-4	1.0420677542033105E-4
26-27	4.7935116693352287E-4	4.89771844475556E-4	0.0	2.1362388961167867E-4	1.0420677542033105E-4
28-29	4.7935116693352287E-4	4.89771844475556E-4	0.0	5.106131995596221E-4	1.0420677542033105E-4
30-31	4.7935116693352287E-4	6.356613300640194E-4	0.0	0.0018913529738790087	1.0420677542033105E-4
32-33	4.7935116693352287E-4	6.356613300640194E-4	0.0	0.005048818269115039	1.1462745296236416E-4
34-35	5.106131995596221E-4	7.45078444255367E-4	0.0	0.009112882510507951	1.2504813050439727E-4
36-37	5.106131995596221E-4	8.440748809046816E-4	0.0	0.01499535498298564	1.2504813050439727E-4
38-39	5.106131995596221E-4	8.440748809046816E-4	0.0	0.024285389011708153	1.3546880804643038E-4
40-41	5.106131995596221E-4	9.482816563250126E-4	0.0	0.033658788460766934	1.3546880804643038E-4
42-43	5.418752321857215E-4	9.743333501800954E-4	0.0	0.043183287734185186	1.4067914681744693E-4
44-45	5.575062484987712E-4	0.001130643513310592	0.0	0.052999565978780375	1.4588948558846347E-4
46-47	5.731372648118208E-4	0.0012504813050439726	0.0	0.0632066196312018	1.6152050190151315E-4
48-49	5.731372648118208E-4	0.0012556916438149891	0.0	0.07315315634507241	1.875721957565959E-4
50-51	6.096096362089367E-4	0.0014172121457165024	0.0	0.08288606916933132	1.9278253452761245E-4
52-53	6.252406525219863E-4	0.0014276328232585354	0.0	0.09170717270866235	2.1362388961167867E-4
54-55	6.40871668835036E-4	0.0014380535008005685	0.0	0.10033549371346576	2.344652446957449E-4
56-57	6.669233626901188E-4	0.0014484741783426017	0.0	0.10856782897167191	2.6051693855082763E-4
58-59	6.877647177741849E-4	0.0014641051946556512	0.0	0.11664385406674757	2.8656863240591037E-4
60-61	6.981853953162181E-4	0.001490156888510734	0.0	0.1239591697012548	2.969893099479435E-4
62-63	7.398681054843505E-4	0.001500577566052767	0.0	0.13123280262559392	3.0219964871896007E-4
64-65	7.45078444255367E-4	0.0016152050190151312	0.0	0.13797498099528932	3.0219964871896007E-4
66-67	7.815508156524829E-4	0.001630836035328181	0.0	0.14494641427090948	3.1262032626099316E-4
68-69	7.815508156524829E-4	0.0016673084067252968	0.0	0.1518449028037354	3.282513425740428E-4
70-71	7.815508156524829E-4	0.0016829394230383466	0.0	0.15814941271666544	3.334616813450594E-4
72-73	8.284438645916319E-4	0.001688149761809363	0.0	0.16345353758556028	3.4388235888709246E-4
74-75	8.336542033626484E-4	0.0017037807781224126	0.0	0.16784064283075623	3.4388235888709246E-4
76-77	8.440748809046816E-4	0.0017454634882905452	0.0	0.17150872132555187	3.4388235888709246E-4
78-79	8.440748809046816E-4	0.001781935859687661	0.0	0.17427020087419065	3.4388235888709246E-4
80-81	8.440748809046816E-4	0.001781935859687661	0.0	0.17511948609386635	3.595133752001421E-4
82-83	8.440748809046816E-4	0.001781935859687661	0.0	0.17572388539130426	3.855650690552249E-4
84-85	8.701265747597642E-4	0.001792356537229694	0.0	0.17600524368493914	3.855650690552249E-4
86-87	8.753369135307809E-4	0.001792356537229694	0.0	0.17609902978281744	3.855650690552249E-4
88	8.85757591072814E-4	0.0019069839901920582	0.0	0.17609902978281744	3.855650690552249E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8955	0.0	42.79804	1
GTATCAA	15745	0.0	34.431736	1
CTTATAC	8815	0.0	33.165726	1
TCAACGC	18005	0.0	29.06373	4
ATCAACG	18150	0.0	28.83154	3
CAACGCA	18430	0.0	28.34908	5
AACGCAG	19000	0.0	27.520157	6
TATACAC	11970	0.0	27.468504	3
TATCAAC	19460	0.0	27.290455	2
TTATACA	13070	0.0	23.652931	2
ACGCAGA	22745	0.0	22.934914	7
CGCAGAG	23795	0.0	21.922865	8
TACACAT	15440	0.0	20.950453	5
GTACATG	24495	0.0	20.744738	1
ACATCTC	14510	0.0	20.656525	8
TACATGG	24580	0.0	20.223227	2
ACACATC	14970	0.0	20.103846	6
ACATGGG	24670	0.0	19.85069	3
GCAGAGT	28565	0.0	18.032671	9
CACATCT	16960	0.0	17.575964	7
>>END_MODULE