FastQCFastQC Report
Fri 27 May 2016
SRR2140198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7617378
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC146950.056.9672931
TATACAC181850.047.1475373
TTATACA183600.046.1360362
GGTATCA67550.040.6042251
TACACAT253000.034.013975
GTATCAA118000.033.4366651
ATACACA277900.031.6376154
ACACATC284750.029.5940766
ACATCTC294200.028.603018
ATCAACG140150.027.387473
TCAACGC140650.027.318334
CACATCT309700.027.0689417
CAACGCA144000.026.6001075
TATCAAC149750.026.0293432
AACGCAG148750.025.8307486
CATCTCC340150.024.6107399
ACGCAGA177200.021.683547
CGCAGAG179300.021.3853028
GTACATG193900.021.3103941
TACATGG190350.021.1656552
Produced by FastQC (version 0.11.5)