##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7617378
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16291327540789	33.0	31.0	34.0	31.0	34.0
2	32.45981596292057	34.0	31.0	34.0	31.0	34.0
3	32.252835030636525	33.0	31.0	34.0	30.0	34.0
4	36.073415944436526	37.0	35.0	37.0	35.0	37.0
5	35.91082180771389	37.0	35.0	37.0	35.0	37.0
6	36.005232246581436	37.0	35.0	37.0	35.0	37.0
7	35.96613335979913	37.0	35.0	37.0	35.0	37.0
8	35.94376424538732	37.0	35.0	37.0	35.0	37.0
9	37.73490681439204	39.0	37.0	39.0	35.0	39.0
10-11	37.65917110848379	39.0	37.0	39.0	35.0	39.0
12-13	37.629874741676204	39.0	37.0	39.0	35.0	39.0
14-15	38.954047705128986	40.0	38.0	41.0	35.5	41.0
16-17	38.922676936867255	40.0	38.0	41.0	35.0	41.0
18-19	38.895446897869576	40.0	38.0	41.0	35.0	41.0
20-21	38.87826817138338	40.0	38.0	41.0	35.0	41.0
22-23	38.78272116201506	40.0	38.0	41.0	34.5	41.0
24-25	38.7107786247882	40.0	38.0	41.0	34.0	41.0
26-27	38.54247603588526	40.0	38.0	41.0	34.0	41.0
28-29	38.40676089090515	40.0	38.0	41.0	34.0	41.0
30-31	38.257507775391645	40.0	38.0	41.0	34.0	41.0
32-33	38.10981348992675	40.0	38.0	41.0	33.5	41.0
34-35	38.22057742823953	40.0	38.0	41.0	34.0	41.0
36-37	38.20316898562319	40.0	38.0	41.0	33.0	41.0
38-39	38.11532643709824	40.0	38.0	41.0	33.0	41.0
40-41	38.12892023261544	40.0	38.0	41.0	33.0	41.0
42-43	38.22752160739007	40.0	38.0	41.0	33.0	41.0
44-45	38.196259590788756	40.0	38.0	41.0	33.0	41.0
46-47	38.114218965373155	40.0	37.5	41.0	33.0	41.0
48-49	38.017949566768195	40.0	37.0	41.0	33.0	41.0
50-51	37.854734345815444	40.0	37.0	41.0	33.0	41.0
52-53	37.71966706693662	40.0	37.0	41.0	33.0	41.0
54-55	37.53742601997601	39.0	36.0	41.0	32.0	41.0
56-57	37.3888514456885	39.0	36.0	41.0	32.0	41.0
58-59	37.19041924473916	39.0	35.0	41.0	31.5	41.0
60-61	36.97941299085038	39.0	35.0	40.5	31.0	41.0
62-63	36.726121285097705	38.0	35.0	40.0	31.0	41.0
64-65	36.453748708608515	38.0	35.0	40.0	31.0	41.0
66-67	36.18226890150497	37.0	35.0	40.0	31.0	41.0
68-69	35.92442696023117	37.0	34.5	39.5	30.0	41.0
70-71	35.634071231242885	36.0	34.0	39.0	30.0	41.0
72-73	35.183072056548575	36.0	34.0	39.0	30.0	40.0
74-75	34.94794086525221	35.0	34.0	38.5	30.0	40.0
76-77	33.719139170366674	34.5	32.5	36.5	28.5	39.0
78-79	34.4303400184111	35.0	34.0	37.0	30.0	39.0
80-81	34.34902982184106	35.0	34.0	36.5	30.0	39.0
82-83	34.11816757804337	35.0	34.0	36.0	30.0	37.5
84-85	33.903779375007105	35.0	34.0	36.0	30.0	37.0
86-87	33.720494029652386	35.0	34.0	35.0	29.5	37.0
88-89	33.549124319547	35.0	33.0	35.0	29.0	36.0
90-91	33.40548939004146	35.0	33.0	35.0	29.0	36.0
92-93	33.322333797752236	35.0	33.0	35.0	29.0	36.0
94-95	33.28425090563208	35.0	33.0	35.0	29.0	35.5
96-97	33.33208433958387	35.0	33.0	35.0	29.5	35.0
98-99	33.41967231661674	35.0	33.0	35.0	30.0	35.0
100	32.24379688151883	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	11.0
20	30.0
21	272.0
22	1202.0
23	3894.0
24	9887.0
25	20949.0
26	37605.0
27	59262.0
28	84032.0
29	111043.0
30	142369.0
31	177510.0
32	220303.0
33	281701.0
34	381116.0
35	535741.0
36	848099.0
37	1506385.0
38	1979469.0
39	1164868.0
40	51628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07844956624182	20.617566832051658	13.969413097262601	25.334570504443914
2	14.607611700509022	20.70922566793981	40.91054690997348	23.772615721577687
3	17.65692867020647	25.789333285022746	31.48844654945573	25.065291495315055
4	12.420599319083285	18.362840862039405	36.84141708603669	32.375142732840615
5	13.102986355672517	37.43526972141858	35.84816192658419	13.613581996324719
6	30.35098166324423	37.20176417659725	18.18361908782786	14.263635072330663
7	26.333273732772618	32.38040701144147	21.985412828403682	19.30090642738223
8	24.92928669156237	36.10363040930882	21.369321569705484	17.597761329423324
9	24.765266998696926	16.354590779136863	20.39041517960642	38.48972704255979
10-11	24.216088528099828	26.18307769418821	28.566522496323536	21.034311281388426
12-13	24.88604346534989	23.469348639387462	28.365830079589067	23.278777815673582
14-15	22.230234603035324	25.321987434521432	25.164288814340054	27.28348914810319
16-17	22.52372798094042	28.886717975660392	25.9176320250879	22.67192201831129
18-19	22.850356119914228	27.37008062354264	27.28879937427288	22.49076388227025
20-21	23.09328138076749	26.82996941651109	27.118309833081728	22.95843936963969
22-23	22.48817180991414	27.397907161895485	27.271148065955437	22.842772962234942
24-25	22.87167414406135	27.170396871445107	27.32397216575836	22.633956818735186
26-27	22.90437416100857	27.478349159195243	27.433324818588634	22.18395186120756
28-29	22.23479221449866	26.8464413752007	27.7578396102449	23.160926800055737
30-31	22.443567272082564	26.93234927160934	28.04823071229574	22.575852744012355
32-33	21.739827103598248	26.596380779614975	28.752048406229143	22.911743710557637
34-35	21.817535249895286	26.949307722491078	28.117556149114968	23.115600878498665
36-37	22.20756611527726	27.070986294586007	28.210457854726474	22.510989735410263
38-39	22.260526543669307	26.671529671018025	28.07281425975146	22.995129525561207
40-41	22.265844294243482	26.46682308250783	28.477621719550715	22.789710903697976
42-43	22.71773887406447	26.31117151716169	28.536656497391178	22.434433111382663
44-45	22.037135439342276	27.04251372571806	28.207343606534003	22.71300722840566
46-47	23.07163320844936	26.226652595981598	27.261216706109952	23.44049748945909
48-49	22.58019601404945	26.265775799141505	27.78541484607099	23.36861334073806
50-51	22.156631365524763	26.797584004979285	28.135774462673492	22.910010166822463
52-53	22.252318804284428	27.20835233699302	28.479927582638993	22.059401276083566
54-55	22.149229415623424	27.327822600247387	27.578849936052208	22.944098048076974
56-57	22.82033040625375	27.983714031501194	26.69030177053703	22.505653791708028
58-59	21.79859696033423	28.61256173549913	27.856859161035114	21.731982143131525
60-61	22.65922424987471	29.128067238058847	26.407756314643045	21.804952197423397
62-63	22.031546709256023	29.628745977334077	26.385634617725973	21.954072695683926
64-65	22.132066704799445	29.129672624974923	26.577220396636235	22.161040273589396
66-67	22.356665325111667	28.536757360434926	26.691344667554183	22.415232646899224
68-69	22.448220557302857	27.979787446770644	27.02165044160524	22.550341554321257
70-71	22.56812173934673	27.418985854613247	27.147833681656014	22.865058724384006
72-73	22.71654243198559	27.40412316437067	27.093835120558435	22.785499283085304
74-75	22.516390203073154	27.5486762395755	27.1086962777845	22.826237279566847
76-77	22.613755934613575	27.47815922127506	27.060473754673005	22.84761108943836
78-79	22.577979445181835	27.465704865100115	27.049916121848316	22.906399567869727
80-81	22.51737171412088	27.515790342517814	27.123559188203433	22.843278755157872
82-83	22.63098401397511	27.48770423858382	27.205513827742678	22.675797919698393
84-85	22.783497258131664	27.463715266679245	27.05002798779452	22.702759487394577
86-87	22.552876271263493	27.48237903330044	27.17644678231833	22.788297913117734
88-89	22.59765422637163	27.4207596894287	27.172559858832695	22.809026225366978
90-91	22.642703473456628	27.478863195031266	27.155726111538574	22.722707219973536
92-93	22.687315400343966	27.43863208597965	27.20241574515866	22.671636768517725
94-95	22.479763264663017	27.524400540502587	27.260196934069302	22.735639260765094
96-97	22.567083450910513	27.629548198416437	27.329777351335157	22.473590999337894
98-99	23.646078332603583	29.64014894664037	27.464870252907055	19.248902467848993
100	30.417811385035655	41.173957375794814	28.408231239169535	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1280.0
1	1172.0
2	1673.0
3	2446.5
4	3130.5
5	4821.5
6	6415.0
7	7490.0
8	8216.5
9	8155.0
10	7649.5
11	7080.5
12	7410.0
13	9368.5
14	14096.0
15	20641.0
16	26331.0
17	30147.0
18	31961.0
19	32088.0
20	31184.0
21	30274.5
22	30818.0
23	34234.5
24	40599.0
25	50010.0
26	62858.0
27	76849.0
28	91653.0
29	108440.5
30	124771.5
31	140924.0
32	157318.0
33	173592.5
34	190367.0
35	207757.5
36	227393.5
37	245456.0
38	258388.0
39	267561.0
40	277462.0
41	288539.5
42	298452.5
43	307958.5
44	318162.5
45	331729.5
46	358098.0
47	387484.5
48	411515.0
49	413880.5
50	392978.0
51	372720.5
52	351393.0
53	326791.0
54	299804.0
55	274488.5
56	252795.0
57	231716.0
58	208146.5
59	180152.0
60	152278.5
61	127172.0
62	103154.5
63	80993.0
64	62235.0
65	48654.5
66	37666.5
67	28929.5
68	22168.0
69	16646.0
70	12431.5
71	9086.0
72	6467.5
73	4427.0
74	3069.5
75	2140.5
76	1427.5
77	955.0
78	650.5
79	427.5
80	286.0
81	190.0
82	131.0
83	93.0
84	62.5
85	41.0
86	29.5
87	22.0
88	13.5
89	5.5
90	3.5
91	2.5
92	1.5
93	1.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005179145935860512
22-23	0.004687267052182346
24-25	0.0036245292380005095
26-27	0.016928073031530162
28-29	0.021097265560334564
30-31	0.038515420926246456
32-33	0.023903659389653723
34-35	0.012545936944799075
36-37	0.007368823552582183
38-39	0.002924758357073863
40-41	0.007874498839504293
42-43	0.009531055448023332
44-45	0.00577514882586593
46-47	0.0022068011904197494
48-49	0.010277406990008037
50-51	0.004745364404136875
52-53	7.402820948561943E-6
54-55	5.551513620188545E-4
56-57	6.858807261038202E-4
58-59	5.354610641815272E-4
60-61	0.0011549535522771925
62-63	0.001103881715448301
64-65	0.0
66-67	2.2690688766613157E-4
68-69	7.129264298577025E-4
70-71	4.116444222487329E-4
72-73	7.559050860673531E-4
74-75	4.420438955001014E-4
76-77	0.0
78-79	9.295522123900386E-6
80-81	4.1552738972398877E-4
82-83	0.0024272410510356693
84-85	9.745052850832378E-6
86-87	6.536971177602672E-4
88-89	3.1244094488188973E-4
90-91	0.008677445388831194
92-93	8.588087965899845E-4
94-95	3.2111597649388235E-5
96-97	2.754631527264682E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	194.0
20-21	561.0
22-23	1221.0
24-25	2835.0
26-27	4999.0
28-29	11950.0
30-31	27959.0
32-33	36445.0
34-35	49678.0
36-37	75025.0
38-39	83626.0
40-41	83215.0
42-43	89365.0
44-45	101240.0
46-47	98234.0
48-49	91247.0
50-51	84286.0
52-53	86691.0
54-55	99466.0
56-57	114562.0
58-59	123199.0
60-61	117850.0
62-63	120341.0
64-65	129877.0
66-67	128793.0
68-69	121146.0
70-71	91099.0
72-73	79713.0
74-75	81043.0
76-77	81397.0
78-79	84794.0
80-81	83603.0
82-83	80610.0
84-85	81695.0
86-87	86142.0
88-89	90537.0
90-91	94733.0
92-93	98809.0
94-95	118002.0
96-97	256920.0
98-99	1436011.0
100-101	2888265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.23938371066956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.96197003809905	79.35466207232152
2	6.532317197086339	11.397506529528412
3	1.4494703575797023	3.7935270210641114
4	0.5025604849595647	1.7537226794083063
5	0.22279153834278592	0.9718098250488326
6	0.11348473758738507	0.5940203140614319
7	0.06835887578990384	0.4174510336545795
8	0.03843398115946018	0.2682365463919033
9	0.026692528033654104	0.20957757258021795
>10	0.08216945720974164	1.1047126609537494
>50	0.0014138621900364628	0.07892892054219683
>100	3.3694196235281473E-4	0.055844824444752125
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3127876810104474E-5	0.0	0.0	0.0
3	0.0	1.3127876810104474E-5	0.0	0.0	0.0
4	0.0	5.2511507240417894E-5	0.0	1.3127876810104474E-5	2.6255753620208947E-5
5	0.0	6.563938405052237E-5	0.0	2.6255753620208947E-5	2.6255753620208947E-5
6	5.2511507240417894E-5	6.563938405052237E-5	0.0	2.6255753620208947E-5	2.6255753620208947E-5
7	5.2511507240417894E-5	9.189513767073131E-5	0.0	3.938363043031342E-5	2.6255753620208947E-5
8	5.2511507240417894E-5	1.3127876810104474E-4	0.0	3.938363043031342E-5	3.938363043031342E-5
9	5.2511507240417894E-5	2.2317390577177606E-4	0.0	3.938363043031342E-5	3.938363043031342E-5
10-11	5.2511507240417894E-5	2.2317390577177606E-4	0.0	3.938363043031342E-5	3.938363043031342E-5
12-13	5.2511507240417894E-5	2.2317390577177606E-4	0.0	3.938363043031342E-5	3.938363043031342E-5
14-15	5.2511507240417894E-5	3.2819692025261185E-4	0.0	5.907544564547013E-5	5.2511507240417894E-5
16-17	5.2511507240417894E-5	3.4132479706271634E-4	0.0	6.563938405052237E-5	6.563938405052237E-5
18-19	5.2511507240417894E-5	3.675805506829253E-4	0.0	7.876726086062684E-5	7.876726086062684E-5
20-21	5.2511507240417894E-5	4.069641811132387E-4	0.0	1.247148296959925E-4	7.876726086062684E-5
22-23	5.2511507240417894E-5	4.397838731384999E-4	0.0	1.444066449111492E-4	9.189513767073131E-5
24-25	5.2511507240417894E-5	5.119871955940745E-4	0.0	2.6255753620208947E-4	1.1815089129094026E-4
26-27	5.2511507240417894E-5	5.448068876193357E-4	0.0	2.888132898222984E-4	1.1815089129094026E-4
28-29	5.2511507240417894E-5	5.513708260243879E-4	0.0	8.730038078719475E-4	1.1815089129094026E-4
30-31	5.907544564547013E-5	6.301380868850147E-4	0.0	0.0028028016989573054	1.247148296959925E-4
32-33	7.876726086062684E-5	6.563938405052237E-4	0.0	0.00668865323474823	1.4440664491114922E-4
34-35	1.0502301448083579E-4	7.614168549860595E-4	0.0	0.011513147962461624	1.5753452172125368E-4
36-37	1.0502301448083579E-4	8.664398694668953E-4	0.0	0.019343926479688946	1.6409846012630593E-4
38-39	1.0502301448083579E-4	9.058234998972087E-4	0.0	0.030843946565340463	1.7066239853135817E-4
40-41	1.0502301448083579E-4	9.977186375679402E-4	0.0	0.04332855741174982	1.7066239853135817E-4
42-43	1.0502301448083579E-4	0.0010239743911881491	0.0	0.05449381663874367	1.7066239853135817E-4
44-45	1.0502301448083579E-4	0.0012012007281245593	0.0	0.06734600803583596	1.7066239853135817E-4
46-47	1.0502301448083579E-4	0.0013652991882508654	0.0	0.08071675056692736	1.7066239853135817E-4
48-49	1.0502301448083579E-4	0.0013718631266559176	0.0	0.09329982048941249	1.8379027534146263E-4
50-51	1.1815089129094026E-4	0.0015556534019973802	0.0	0.10497706691200043	1.8379027534146263E-4
52-53	1.1815089129094026E-4	0.0015622173404024324	0.0	0.11635237216795596	1.8379027534146263E-4
54-55	1.1815089129094026E-4	0.0015687812788074847	0.0	0.12732071324279826	1.9035421374651487E-4
56-57	1.1815089129094026E-4	0.0015884730940226414	0.0	0.13773768349161614	1.9691815215156712E-4
58-59	1.1815089129094026E-4	0.0015884730940226414	0.0	0.14763610260643492	2.1004602896167158E-4
60-61	1.1815089129094026E-4	0.0016147288476428504	0.0	0.15681248849669793	2.1004602896167158E-4
62-63	1.3127876810104474E-4	0.0016212927860479026	0.0	0.16504366725663344	2.1004602896167158E-4
64-65	1.3127876810104474E-4	0.0017853912461742085	0.0	0.17372119382811252	2.1004602896167158E-4
66-67	1.3127876810104474E-4	0.0018116469997944175	0.0	0.18265471399738858	2.1660996736672382E-4
68-69	1.3127876810104474E-4	0.001824774876604522	0.0	0.1914635193369687	2.2317390577177606E-4
70-71	1.444066449111492E-4	0.0018379027534146265	0.0	0.19784366746667947	2.2317390577177606E-4
72-73	1.7066239853135817E-4	0.0018379027534146265	0.0	0.20359367750950524	2.4942965939198504E-4
74-75	1.7066239853135817E-4	0.0018904142606550444	0.0	0.2082868934691176	2.4942965939198504E-4
76-77	1.7066239853135817E-4	0.0019494897063005146	0.0	0.2127241158309329	2.559935977970373E-4
78-79	1.7066239853135817E-4	0.0019954372751358803	0.0	0.21530374362411842	2.6255753620208947E-4
80-81	1.7066239853135817E-4	0.002028256967161141	0.0	0.21619643924720555	2.822493514172462E-4
82-83	1.7066239853135817E-4	0.002047948782376298	0.0	0.21682657733409055	3.0194116663240293E-4
84-85	1.7066239853135817E-4	0.002107024228021768	0.0	0.21703005942464715	3.085051050374551E-4
86-87	1.7066239853135817E-4	0.0021398439200470292	0.0	0.21711539062391283	3.1506904344250736E-4
88	1.7066239853135817E-4	0.002297378441768283	0.0	0.2171219545623179	3.1506904344250736E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	14695	0.0	56.967293	1
TATACAC	18185	0.0	47.147537	3
TTATACA	18360	0.0	46.136036	2
GGTATCA	6755	0.0	40.604225	1
TACACAT	25300	0.0	34.01397	5
GTATCAA	11800	0.0	33.436665	1
ATACACA	27790	0.0	31.637615	4
ACACATC	28475	0.0	29.594076	6
ACATCTC	29420	0.0	28.60301	8
ATCAACG	14015	0.0	27.38747	3
TCAACGC	14065	0.0	27.31833	4
CACATCT	30970	0.0	27.068941	7
CAACGCA	14400	0.0	26.600107	5
TATCAAC	14975	0.0	26.029343	2
AACGCAG	14875	0.0	25.830748	6
CATCTCC	34015	0.0	24.610739	9
ACGCAGA	17720	0.0	21.68354	7
CGCAGAG	17930	0.0	21.385302	8
GTACATG	19390	0.0	21.310394	1
TACATGG	19035	0.0	21.165655	2
>>END_MODULE