FastQCFastQC Report
Fri 27 May 2016
SRR2140197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6914805
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68550.040.4351731
GTATCAA116800.032.677671
CTTATAC64350.030.7407971
ATCAACG130600.027.972693
TCAACGC131200.027.8143714
CAACGCA133000.027.3479735
AACGCAG137200.026.5979986
TATCAAC141750.026.250682
TATACAC92700.024.2220343
ACGCAGA161350.022.5922347
CGCAGAG166400.021.9784978
TTATACA96400.021.927082
GTACATG169500.019.6235831
TACATGG167400.019.5123882
ACATCTC107050.019.1122888
ACATGGG168650.018.8475073
TACACAT117800.018.7224125
GCAGAGT202300.018.1964959
ACACATC111450.018.071466
GAGTACT113300.016.79076412-13
Produced by FastQC (version 0.11.5)