##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6914805
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19623329942059	33.0	31.0	34.0	31.0	34.0
2	32.48219450873886	34.0	31.0	34.0	31.0	34.0
3	32.2819937800126	33.0	31.0	34.0	30.0	34.0
4	36.09107964722071	37.0	35.0	37.0	35.0	37.0
5	35.93384527835565	37.0	35.0	37.0	35.0	37.0
6	36.028809344587444	37.0	35.0	37.0	35.0	37.0
7	35.995297336656634	37.0	35.0	37.0	35.0	37.0
8	35.9738433694081	37.0	35.0	37.0	35.0	37.0
9	37.76678590357935	39.0	38.0	39.0	35.0	39.0
10-11	37.69474301878361	39.0	37.0	39.0	35.0	39.0
12-13	37.668400338115106	39.0	37.0	39.0	35.0	39.0
14-15	39.005664006432575	40.0	38.0	41.0	35.5	41.0
16-17	38.98559872621137	40.0	38.0	41.0	35.5	41.0
18-19	38.95685988252742	40.0	38.0	41.0	35.0	41.0
20-21	38.952026936103465	40.0	38.0	41.0	35.0	41.0
22-23	38.86369580865033	40.0	38.0	41.0	35.0	41.0
24-25	38.80104697918398	40.0	38.0	41.0	35.0	41.0
26-27	38.65645398574812	40.0	38.0	41.0	34.0	41.0
28-29	38.53839367588526	40.0	38.0	41.0	34.0	41.0
30-31	38.39395692070087	40.0	38.0	41.0	34.0	41.0
32-33	38.2462384830291	40.0	38.0	41.0	34.0	41.0
34-35	38.3430672369891	40.0	38.0	41.0	34.0	41.0
36-37	38.33662172656521	40.0	38.0	41.0	34.0	41.0
38-39	38.27022547068633	40.0	38.0	41.0	34.0	41.0
40-41	38.26352546038244	40.0	38.0	41.0	33.5	41.0
42-43	38.3438549334167	40.0	38.0	41.0	34.0	41.0
44-45	38.30993152525434	40.0	38.0	41.0	33.5	41.0
46-47	38.23629000187864	40.0	38.0	41.0	33.0	41.0
48-49	38.14731733429586	40.0	37.5	41.0	33.0	41.0
50-51	38.03534380888961	40.0	37.0	41.0	33.0	41.0
52-53	37.89544981382821	40.0	37.0	41.0	33.0	41.0
54-55	37.739173821463005	40.0	37.0	41.0	33.0	41.0
56-57	37.62732390550759	39.5	36.0	41.0	33.0	41.0
58-59	37.428401858023975	39.0	36.0	41.0	32.0	41.0
60-61	37.23002986207616	39.0	35.5	41.0	32.0	41.0
62-63	36.976849493169496	39.0	35.0	40.5	31.0	41.0
64-65	36.681431726883176	38.0	35.0	40.0	31.0	41.0
66-67	36.39966782383344	37.5	35.0	40.0	31.0	41.0
68-69	36.125369566721346	37.0	35.0	40.0	31.0	41.0
70-71	35.82110775288694	36.5	34.5	39.0	30.5	41.0
72-73	35.36242860463537	36.0	34.0	39.0	30.0	40.5
74-75	35.11972484120151	35.5	34.0	38.5	30.0	40.0
76-77	33.87055118048575	34.5	32.5	36.5	28.5	39.0
78-79	34.56966808948117	35.0	34.0	37.0	30.0	39.0
80-81	34.4734924703494	35.0	34.0	37.0	30.0	39.0
82-83	34.227534508723096	35.0	34.0	36.0	30.0	38.5
84-85	34.00458911436478	35.0	34.0	36.0	30.0	37.0
86-87	33.81453970108299	35.0	34.0	35.5	30.0	37.0
88-89	33.63601119561056	35.0	34.0	35.0	30.0	36.5
90-91	33.484765597335034	35.0	33.0	35.0	29.5	36.0
92-93	33.39778204626982	35.0	33.0	35.0	29.0	36.0
94-95	33.35536812351238	35.0	33.0	35.0	29.0	36.0
96-97	33.39575608401696	35.0	33.0	35.0	30.0	35.0
98-99	33.47641826107948	35.0	33.0	35.0	30.0	35.0
100	32.32569164679576	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	33.0
21	202.0
22	963.0
23	3072.0
24	7962.0
25	17220.0
26	30630.0
27	48569.0
28	70332.0
29	94931.0
30	121378.0
31	153274.0
32	192328.0
33	245759.0
34	332484.0
35	467721.0
36	741837.0
37	1343975.0
38	1824783.0
39	1164058.0
40	53288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28746942249275	20.386099101854644	13.801083327729415	24.525348147923186
2	14.95083375453104	21.12110175196553	40.62224458968836	23.305819903815074
3	18.122000548099333	25.68940121955717	30.7968915970877	25.3917066352558
4	12.679027101993476	17.852781097948533	38.28609772799088	31.182094072067105
5	13.219505105350043	37.65471332886466	35.85774291538228	13.268038650403014
6	30.509956535289135	37.617431004923496	18.41592062249044	13.45669183729693
7	26.727868681763262	32.40024845241478	22.88666130136714	17.985221564454818
8	24.668605983827455	36.44331257352882	21.306327510320248	17.581753932323473
9	25.276287617655164	16.6658495792723	21.21383032493324	36.8440324781393
10-11	24.325863129907496	26.58574609117683	28.897987723442668	20.190403055473002
12-13	24.861243375626643	24.002860528966472	28.914654860115363	22.221241235291522
14-15	22.059407315173747	25.626507182776663	26.230313074627553	26.08377242742203
16-17	21.9427735127744	28.797746284963928	27.065181158398538	22.19429904386313
18-19	22.028994310034776	27.978431785133495	28.463412923430237	21.5291609814015
20-21	22.669567655350164	27.29153048847556	28.237888014287133	21.801013841887148
22-23	22.29966411711575	27.361021342011338	28.12118571727879	22.218128823594117
24-25	22.229168035012684	27.359489068518762	28.268659152072022	22.142683744396532
26-27	22.237734726337063	27.5499733056429	28.196492758798303	22.015799209221736
28-29	22.042270110080132	27.5838277358355	28.22483907531762	22.14906307876675
30-31	22.305923688648456	27.43528417835358	28.243124258196623	22.01566787480134
32-33	21.938977525862168	27.46872922570939	28.34614050126541	22.246152747163034
34-35	22.068239394143465	27.52105518825882	28.23486254505676	22.175842872540954
36-37	22.138188091603507	27.545243797694773	28.24277188559089	22.073796225110833
38-39	21.99949487564636	27.591544080911902	28.254590739496376	22.154370303945363
40-41	22.243425180740356	27.399039427375005	28.23059547612105	22.12693991576359
42-43	22.272031867291435	27.431017909131334	28.08354024160602	22.213409981971207
44-45	22.142108575227535	27.717679183937065	27.89838477267063	22.241827468164765
46-47	22.430891205930216	27.43671311939675	27.569490700884046	22.56290497378899
48-49	22.252753706566946	27.558376182086224	27.702427896236102	22.486442215110728
50-51	22.157156746904068	27.65284140961313	27.798044029108237	22.391957814374567
52-53	22.331870199476633	27.690988361169193	27.732996914832746	22.244144524521424
54-55	22.284033429941875	27.597123347897103	27.607534551349893	22.511308670811133
56-57	22.470211999993925	27.868052286659488	27.321744155607647	22.33999155773894
58-59	22.14022861035964	28.07127915289286	27.697732813612713	22.090759423134788
60-61	22.443678186110674	28.14692226623453	27.285664365282585	22.123735182372208
62-63	22.296197261488825	28.340487434442313	27.22559857822942	22.13771672583944
64-65	22.258437292341497	28.17952358910961	27.272424205627114	22.289614912921778
66-67	22.38721640091116	28.073530751708432	27.179927107061502	22.359325740318905
68-69	22.316446557807428	27.941484028189034	27.3804684402323	22.361600973771242
70-71	22.31845988514328	27.572525211962436	27.484356448548645	22.624658454345635
72-73	22.42190022014129	27.632019052846623	27.383381469225387	22.5626992577867
74-75	22.27547249676667	27.811755138748488	27.360398440087042	22.552373924397802
76-77	22.36978102079078	27.746384232764225	27.28595797408901	22.59787677235599
78-79	22.29871615161962	27.76959025557939	27.29277572472908	22.63891786807191
80-81	22.323545606246388	27.768611069351852	27.30087007326692	22.606973251134843
82-83	22.39631249775992	27.743866256361187	27.445639739930527	22.414181505948367
84-85	22.505102965370348	27.719754889775633	27.299902006695948	22.475240138158075
86-87	22.30032113865681	27.74649091014039	27.401826198039025	22.551361753163775
88-89	22.361291454869846	27.765580288896587	27.341034548820097	22.532093707413473
90-91	22.36034477147588	27.79107333843558	27.39028413560173	22.458297754486807
92-93	22.445941416538602	27.720365331836234	27.41402178746536	22.419671464159805
94-95	22.246643957068773	27.83428509249636	27.477336462105278	22.44173448832959
96-97	22.275798943552587	27.970695552953938	27.525367270800626	22.22813823269285
98-99	23.3421822272216	29.901944865587453	27.746518641691527	19.00935426549942
100	29.880143627237217	41.503149964278755	28.616706408484028	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1071.0
1	1041.5
2	1554.5
3	2230.5
4	2930.5
5	4655.0
6	6176.5
7	7079.0
8	7578.5
9	7455.0
10	6944.0
11	6422.5
12	6924.5
13	8893.5
14	13456.5
15	19695.0
16	25183.0
17	28975.0
18	30579.0
19	30537.0
20	29968.0
21	29573.0
22	30444.5
23	33735.5
24	39865.0
25	49230.0
26	62783.5
27	77655.0
28	92426.5
29	109420.5
30	125139.5
31	139907.5
32	156502.0
33	173250.0
34	189768.5
35	206414.0
36	225026.5
37	240446.5
38	250345.5
39	259892.0
40	271666.5
41	283981.0
42	291925.5
43	298011.5
44	305134.5
45	311514.5
46	319275.5
47	326721.5
48	331162.0
49	327751.0
50	317735.5
51	307117.0
52	292513.5
53	274291.5
54	256255.0
55	237967.5
56	219212.5
57	199809.5
58	178495.5
59	154619.0
60	130218.5
61	107034.0
62	85520.5
63	67134.5
64	51357.5
65	39200.5
66	30075.0
67	23018.0
68	17479.0
69	12969.5
70	9442.0
71	6815.5
72	4855.0
73	3381.5
74	2365.5
75	1655.5
76	1127.0
77	740.5
78	479.0
79	311.0
80	209.0
81	138.0
82	94.5
83	68.5
84	50.0
85	36.5
86	28.0
87	20.0
88	15.5
89	14.5
90	11.0
91	8.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.004902716864413196
22-23	0.004584974806141986
24-25	0.0036455748941408954
26-27	0.01663552059193799
28-29	0.021002141059649267
30-31	0.038799947947394935
32-33	0.023633369268006122
34-35	0.012646425791088519
36-37	0.007096707445287967
38-39	0.002441922385849276
40-41	0.007270352831280958
42-43	0.008889660040121285
44-45	0.005545663849314308
46-47	0.001866190223840462
48-49	0.009419845385102694
50-51	0.004146854367982137
52-53	2.4614380853556166E-5
54-55	3.575720029437323E-4
56-57	5.99157281064765E-4
58-59	3.5983692190699176E-4
60-61	8.348992041845147E-4
62-63	8.914230014847929E-4
64-65	0.0
66-67	2.2778991391819153E-4
68-69	4.7257630439399595E-4
70-71	2.2602494543145664E-4
72-73	7.456204121044017E-4
74-75	3.6857977676480846E-4
76-77	0.0
78-79	0.0
80-81	4.5740158090183734E-4
82-83	0.0025718315654831695
84-85	6.295743164737878E-5
86-87	5.654756824117858E-4
88-89	2.280688702825719E-4
90-91	0.009673706114136451
92-93	0.0010837507937627884
94-95	3.461710481920871E-5
96-97	2.851081254746605E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	184.0
20-21	511.0
22-23	1154.0
24-25	2206.0
26-27	4599.0
28-29	10687.0
30-31	26733.0
32-33	34248.0
34-35	47114.0
36-37	71415.0
38-39	80114.0
40-41	79682.0
42-43	85927.0
44-45	96245.0
46-47	93779.0
48-49	86815.0
50-51	79904.0
52-53	79498.0
54-55	86637.0
56-57	89249.0
58-59	87225.0
60-61	85212.0
62-63	84738.0
64-65	90178.0
66-67	91719.0
68-69	89178.0
70-71	80515.0
72-73	75433.0
74-75	77672.0
76-77	77588.0
78-79	79700.0
80-81	79026.0
82-83	75608.0
84-85	77817.0
86-87	81458.0
88-89	85504.0
90-91	88118.0
92-93	92315.0
94-95	110094.0
96-97	235233.0
98-99	1319298.0
100-101	2694475.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.96107459107097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.01622580147902	79.14868800920176
2	6.288464250578076	10.937032175156066
3	1.539088766864356	4.015224391727122
4	0.544652880410298	1.8945439903840635
5	0.25682380880292205	1.116683719703693
6	0.12071803201699788	0.6298661872030469
7	0.07047708185216661	0.42901339403350924
8	0.04478355918579904	0.3115541144647929
9	0.03000130718131278	0.23480513204613965
>10	0.08721726059300768	1.1070078305884266
>50	0.0011126199410614835	0.06399575985969647
>100	3.6044319436254066E-4	0.05651146576206178
>500	4.945875195089935E-5	0.02298876760285168
>1k	2.4729148672422277E-5	0.03208506226676086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4461723794091085E-5	0.0	2.892344758818217E-5	0.0
3	0.0	1.4461723794091085E-5	0.0	2.892344758818217E-5	0.0
4	0.0	2.892344758818217E-5	0.0	2.892344758818217E-5	0.0
5	0.0	4.3385171382273254E-5	0.0	2.892344758818217E-5	0.0
6	8.677034276454651E-5	4.3385171382273254E-5	0.0	2.892344758818217E-5	0.0
7	8.677034276454651E-5	4.3385171382273254E-5	0.0	2.892344758818217E-5	0.0
8	8.677034276454651E-5	5.784689517636434E-5	0.0	2.892344758818217E-5	0.0
9	8.677034276454651E-5	1.1569379035272868E-4	0.0	8.677034276454651E-5	0.0
10-11	8.677034276454651E-5	1.1569379035272868E-4	0.0	8.677034276454651E-5	0.0
12-13	8.677034276454651E-5	1.1569379035272868E-4	0.0	1.1569379035272868E-4	0.0
14-15	1.0123206655863758E-4	1.3015551414681975E-4	0.0	1.2292465224977421E-4	0.0
16-17	1.880024093231841E-4	1.3015551414681975E-4	0.0	1.6630982363204745E-4	0.0
18-19	1.880024093231841E-4	1.5184809983795637E-4	0.0	1.880024093231841E-4	7.230861897045542E-6
20-21	1.880024093231841E-4	1.5907896173500194E-4	0.0	1.880024093231841E-4	1.4461723794091085E-5
22-23	2.0246413311727517E-4	2.0969499501432074E-4	0.0	2.3138758070545735E-4	1.4461723794091085E-5
24-25	2.0246413311727517E-4	2.386184426025029E-4	0.0	2.820036139847762E-4	1.4461723794091085E-5
26-27	2.0246413311727517E-4	2.603110282936395E-4	0.0	3.832356805434137E-4	1.4461723794091085E-5
28-29	2.1692585691136627E-4	2.603110282936395E-4	0.0	9.400120466159204E-4	1.4461723794091085E-5
30-31	2.1692585691136627E-4	2.603110282936395E-4	0.0	0.0032321952679793572	1.4461723794091085E-5
32-33	2.241567188084118E-4	2.603110282936395E-4	0.0	0.007700867920353502	1.4461723794091085E-5
34-35	2.3138758070545735E-4	3.253887853670494E-4	0.0	0.013731406742489485	1.4461723794091085E-5
36-37	2.4584930449954843E-4	3.9046654244045926E-4	0.0	0.022314439814282543	1.4461723794091085E-5
38-39	2.4584930449954843E-4	4.0492826623455034E-4	0.0	0.0355975331191552	1.4461723794091085E-5
40-41	2.4584930449954843E-4	4.0492826623455034E-4	0.0	0.04847569815779332	1.4461723794091085E-5
42-43	2.4584930449954843E-4	4.2662085192568696E-4	0.0	0.06133217061074029	1.4461723794091085E-5
44-45	2.5308016639659397E-4	4.772368852050058E-4	0.0	0.07498403787236227	1.4461723794091085E-5
46-47	2.603110282936395E-4	5.061603327931879E-4	0.0	0.08977838131371746	1.4461723794091085E-5
48-49	2.603110282936395E-4	5.061603327931879E-4	0.0	0.10434856803626422	1.4461723794091085E-5
50-51	2.6754189019068505E-4	5.495455041754612E-4	0.0	0.11751596755078415	1.4461723794091085E-5
52-53	2.747727520877306E-4	5.495455041754612E-4	0.0	0.1306978287890982	1.4461723794091085E-5
54-55	2.747727520877306E-4	5.640072279695522E-4	0.0	0.1427661372952672	1.4461723794091085E-5
56-57	2.747727520877306E-4	5.929306755577344E-4	0.0	0.15434997805433415	1.4461723794091085E-5
58-59	2.747727520877306E-4	5.929306755577344E-4	0.0	0.16476965004797678	1.4461723794091085E-5
60-61	3.1815792347000387E-4	6.073923993518256E-4	0.0	0.17420592482362118	1.4461723794091085E-5
62-63	3.615430948522771E-4	6.146232612488712E-4	0.0	0.18366389218495677	1.4461723794091085E-5
64-65	3.615430948522771E-4	7.809330848809185E-4	0.0	0.1933387854032037	1.4461723794091085E-5
66-67	3.760048186463682E-4	7.809330848809185E-4	0.0	0.2027967527645393	1.4461723794091085E-5
68-69	3.760048186463682E-4	7.953948086750096E-4	0.0	0.21108332049855347	1.4461723794091085E-5
70-71	3.760048186463682E-4	8.170873943661463E-4	0.0	0.21850218480492217	1.4461723794091085E-5
72-73	4.3385171382273255E-4	8.315491181602373E-4	0.0	0.2242362582892793	1.4461723794091085E-5
74-75	4.3385171382273255E-4	8.387799800572829E-4	0.0	0.22878447042252095	1.4461723794091085E-5
76-77	4.3385171382273255E-4	8.53241703851374E-4	0.0	0.23345560720801237	1.4461723794091085E-5
78-79	4.3385171382273255E-4	8.53241703851374E-4	0.0	0.23677457281875627	1.4461723794091085E-5
80-81	4.3385171382273255E-4	8.53241703851374E-4	0.0	0.23772181572726925	1.4461723794091085E-5
82-83	4.3385171382273255E-4	8.53241703851374E-4	0.0	0.23848105622645904	1.4461723794091085E-5
84-85	4.3385171382273255E-4	8.53241703851374E-4	0.0	0.23874136725475265	1.4461723794091085E-5
86-87	4.410825757197781E-4	8.821651514395562E-4	0.0	0.2388570610451054	1.4461723794091085E-5
88	4.772368852050058E-4	9.544737704100116E-4	0.0	0.23886429190700245	1.4461723794091085E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6855	0.0	40.435173	1
GTATCAA	11680	0.0	32.67767	1
CTTATAC	6435	0.0	30.740797	1
ATCAACG	13060	0.0	27.97269	3
TCAACGC	13120	0.0	27.814371	4
CAACGCA	13300	0.0	27.347973	5
AACGCAG	13720	0.0	26.597998	6
TATCAAC	14175	0.0	26.25068	2
TATACAC	9270	0.0	24.222034	3
ACGCAGA	16135	0.0	22.592234	7
CGCAGAG	16640	0.0	21.978497	8
TTATACA	9640	0.0	21.92708	2
GTACATG	16950	0.0	19.623583	1
TACATGG	16740	0.0	19.512388	2
ACATCTC	10705	0.0	19.112288	8
ACATGGG	16865	0.0	18.847507	3
TACACAT	11780	0.0	18.722412	5
GCAGAGT	20230	0.0	18.196495	9
ACACATC	11145	0.0	18.07146	6
GAGTACT	11330	0.0	16.790764	12-13
>>END_MODULE