FastQCFastQC Report
Fri 27 May 2016
SRR2140196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7347010
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.040.159041
GTATCAA114650.032.5777361
TCAACGC124500.028.7406234
ATCAACG127750.028.0417583
CAACGCA130000.027.5881675
AACGCAG135050.026.6787856
TATCAAC141000.025.9627632
ACGCAGA162000.022.1896047
CGCAGAG167500.021.485638
GTACATG179450.020.8138071
TACATGG177050.020.3267022
GTGGTAT41400.019.638681
ACATGGG181400.019.406993
TGGTATC38650.018.6868022
ACCGAGG108150.017.90804590-91
GCAGAGT205100.017.6675079
CATGGGG136800.017.6186714
GACCGAG106500.017.60817790-91
AGACCGA106950.017.55240888-89
CGAGACC106150.017.35887586-87
Produced by FastQC (version 0.11.5)