##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7347010
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15299502790931	33.0	31.0	34.0	31.0	34.0
2	32.44255336524654	34.0	31.0	34.0	31.0	34.0
3	32.23704758806644	33.0	31.0	34.0	30.0	34.0
4	36.06091947608619	37.0	35.0	37.0	35.0	37.0
5	35.896533555827475	37.0	35.0	37.0	35.0	37.0
6	35.99529427617493	37.0	35.0	37.0	35.0	37.0
7	35.96450746630262	37.0	35.0	37.0	35.0	37.0
8	35.93832511457042	37.0	35.0	37.0	35.0	37.0
9	37.728654377767285	39.0	37.0	39.0	35.0	39.0
10-11	37.649576086054054	39.0	37.0	39.0	35.0	39.0
12-13	37.62343946721183	39.0	37.0	39.0	35.0	39.0
14-15	38.94684790139118	40.0	38.0	41.0	35.5	41.0
16-17	38.925331393315105	40.0	38.0	41.0	35.0	41.0
18-19	38.89223786274961	40.0	38.0	41.0	35.0	41.0
20-21	38.884633058720624	40.0	38.0	41.0	35.0	41.0
22-23	38.79481374565401	40.0	38.0	41.0	34.5	41.0
24-25	38.730760245005136	40.0	38.0	41.0	34.0	41.0
26-27	38.58090756047447	40.0	38.0	41.0	34.0	41.0
28-29	38.45107511961106	40.0	38.0	41.0	34.0	41.0
30-31	38.30463711675195	40.0	38.0	41.0	34.0	41.0
32-33	38.148889571609175	40.0	38.0	41.0	33.5	41.0
34-35	38.25060230809162	40.0	38.0	41.0	34.0	41.0
36-37	38.24766369269439	40.0	38.0	41.0	34.0	41.0
38-39	38.174505362471784	40.0	38.0	41.0	33.0	41.0
40-41	38.160491416241996	40.0	38.0	41.0	33.0	41.0
42-43	38.234946121625775	40.0	38.0	41.0	33.0	41.0
44-45	38.1942295688289	40.0	38.0	41.0	33.0	41.0
46-47	38.109452949558175	40.0	37.5	41.0	33.0	41.0
48-49	38.01250996451269	40.0	37.0	41.0	33.0	41.0
50-51	37.89803799717998	40.0	37.0	41.0	33.0	41.0
52-53	37.74900304147367	40.0	37.0	41.0	33.0	41.0
54-55	37.5890594689687	40.0	36.0	41.0	32.0	41.0
56-57	37.48217249443876	39.0	36.0	41.0	32.0	41.0
58-59	37.276968687460545	39.0	36.0	41.0	32.0	41.0
60-61	37.08186660818653	39.0	35.0	41.0	31.0	41.0
62-63	36.82107966631537	38.5	35.0	40.0	31.0	41.0
64-65	36.52084708527253	38.0	35.0	40.0	31.0	41.0
66-67	36.23695274524682	37.5	35.0	40.0	31.0	41.0
68-69	35.95326833256462	37.0	34.5	39.5	30.0	41.0
70-71	35.63826590813815	36.5	34.0	39.0	30.0	41.0
72-73	35.16645187389706	36.0	34.0	39.0	29.0	40.0
74-75	34.92440415550806	35.5	34.0	38.5	29.0	40.0
76-77	33.7007898305655	34.5	32.0	36.5	28.5	39.0
78-79	34.41098225069847	35.0	34.0	37.0	29.5	39.0
80-81	34.309632248268585	35.0	34.0	37.0	30.0	39.0
82-83	34.06306733020024	35.0	34.0	36.0	29.0	38.0
84-85	33.839544730144254	35.0	34.0	36.0	29.0	37.0
86-87	33.650895011909256	35.0	33.0	35.5	29.0	37.0
88-89	33.465405883890284	35.0	33.0	35.0	29.0	36.5
90-91	33.314450456234965	35.0	33.0	35.0	29.0	36.0
92-93	33.228565483497434	35.0	33.0	35.0	29.0	36.0
94-95	33.185193390676666	35.0	33.0	35.0	29.0	36.0
96-97	33.23984087137016	35.0	33.0	35.0	29.0	35.0
98-99	33.350007591160626	35.0	33.0	35.0	29.5	35.0
100	32.138919644637774	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	30.0
21	211.0
22	979.0
23	3540.0
24	9236.0
25	20014.0
26	36339.0
27	58128.0
28	83275.0
29	112354.0
30	143637.0
31	177372.0
32	220350.0
33	280322.0
34	375447.0
35	526522.0
36	832085.0
37	1479811.0
38	1910926.0
39	1036319.0
40	40107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.638734668933346	20.121028826692765	13.703125489144563	24.537111015229325
2	15.144990955504348	20.966815071709444	40.450999794474214	23.437194178311994
3	18.57909544154697	25.23180178058829	30.218265661813444	25.970837116051293
4	12.767615669503648	17.560803646653536	38.09726677927483	31.57431390456798
5	13.44941683759788	37.50634611903346	35.6107314404091	13.433505602959572
6	31.059165565311602	37.32402705318218	18.236588761958945	13.380218619547271
7	27.31113745591744	32.03918328680647	22.531192961490458	18.118486295785633
8	24.85127963620575	36.64961664677195	20.86488244877848	17.634221268243817
9	25.26732643619649	16.73848272970909	21.022388699620663	36.971802134473755
10-11	24.49428406930166	26.60107989508657	28.59832775510037	20.30630828051139
12-13	24.99948958828149	23.869138601961886	28.82987092708462	22.301500882672
14-15	22.05599284606935	25.533666076403872	26.14194754056412	26.268393536962652
16-17	22.079805254110173	28.781531534597065	26.881425232849825	22.25723797844293
18-19	22.09653995298768	27.982601085339475	28.265525703653594	21.655333258019247
20-21	22.717295922871177	27.22048617692698	28.207812074512834	21.854405825689014
22-23	22.471385769126105	27.25489750308104	27.98504247295675	22.288674254836106
24-25	22.28266452736988	27.304388696828276	28.19049081761453	22.222455958187314
26-27	22.347374741756997	27.493259050526675	28.044304595833186	22.115061611883142
28-29	22.186823688925685	27.448938354135876	28.048186900984057	22.316051055954382
30-31	22.52927529688099	27.334950806247356	28.03451501181624	22.10125888505542
32-33	22.381881187840786	27.407936750256646	28.06490936559926	22.145272696303305
34-35	22.26897366506268	27.469642122147715	28.175195219915754	22.086188992873854
36-37	22.386671021655836	27.435069089800347	27.94817064861809	22.230089239925732
38-39	22.11837595506419	27.514477729893628	28.11865094072371	22.248495374318473
40-41	22.38411098658671	27.35628872407359	28.06621863227709	22.19338165706261
42-43	22.285854230693896	27.434013160269938	27.9748054770875	22.305327131948662
44-45	22.269227081779576	27.569600917515196	27.828337904990207	22.332834095715018
46-47	22.446599388150346	27.44819603892558	27.593489443032265	22.51171512989181
48-49	22.33162465329607	27.5078572258585	27.647284811308616	22.51323330953681
50-51	22.232040425971032	27.66654249866533	27.664937774595405	22.43647930076823
52-53	22.402025206608688	27.650647659075016	27.642305819490844	22.305021314825453
54-55	22.354555462698688	27.542848223979853	27.583383655356396	22.51921265796506
56-57	22.43118310372666	27.684483204985423	27.46140754278221	22.422926148505706
58-59	22.175137628898455	27.756305921704875	27.56171027411371	22.50684617528296
60-61	22.51078440415728	27.78524337347743	27.54177390981883	22.16219831254646
62-63	22.180200092497106	27.889993811946656	27.511486741791753	22.41831935376449
64-65	22.166778109599296	27.794636888412143	27.502890979576588	22.53569402241197
66-67	22.22661923288055	27.782910020390013	27.633143810669107	22.35732693606033
68-69	22.217297720443757	27.977120844869667	27.494815614196465	22.310765820490108
70-71	22.22756868860229	27.712644700161615	27.273288032823316	22.78649857841278
72-73	22.297617436889517	27.68793642016342	27.212112910179275	22.802333232767786
74-75	22.19315782621787	27.807322612159613	27.492597209903018	22.506922351719496
76-77	22.3564034481711	27.512875645632697	27.371874190175443	22.758846716020763
78-79	22.23351895080447	27.54119091205009	27.11393229138297	23.11135784576247
80-81	22.506339222385776	27.848914235670964	27.139670262403314	22.505076279539946
82-83	22.58270547954401	27.56407169678855	27.221925765959547	22.63129705770789
84-85	22.429305871803496	27.53318566918437	27.132104040023293	22.905404418988848
86-87	22.274738293566116	27.824407766858183	27.237107104775067	22.663746834800634
88-89	22.569705421836623	27.815359559162093	27.23211611040881	22.38281890859247
90-91	22.610692489458874	27.865961249493125	27.20603680442377	22.317309456624226
92-93	22.33250404730715	27.537754269754565	27.27858951345807	22.851152169480216
94-95	22.46422196520132	27.883549111713513	27.37900305591787	22.273225867167294
96-97	22.74973680688176	27.734348185207768	27.450257563752068	22.06565744415841
98-99	23.387182225020943	29.859510863149836	27.870535748751568	18.882771163077656
100	29.88622263670475	41.42948338505706	28.684293978238184	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1109.0
1	1005.0
2	1376.5
3	1965.0
4	2537.0
5	4005.0
6	5345.0
7	6318.5
8	6978.5
9	6853.5
10	6301.0
11	5822.0
12	6262.5
13	8072.0
14	12466.0
15	18530.5
16	23714.5
17	27115.5
18	28470.5
19	28289.0
20	27559.0
21	27118.5
22	27907.5
23	31081.5
24	37446.0
25	46810.5
26	59597.5
27	74452.5
28	89436.5
29	105605.0
30	120751.0
31	135768.0
32	151982.5
33	168225.5
34	184797.0
35	200837.5
36	218814.5
37	235491.5
38	249228.0
39	262360.5
40	276043.0
41	289229.5
42	298499.5
43	306670.0
44	315412.5
45	322091.5
46	327265.0
47	333269.0
48	338463.0
49	338641.5
50	334231.0
51	325455.0
52	308847.0
53	289182.0
54	269407.5
55	247814.0
56	226505.5
57	205640.5
58	183259.0
59	158298.5
60	133537.5
61	110318.5
62	88672.5
63	69891.0
64	53905.5
65	41898.5
66	32707.5
67	24461.5
68	17962.5
69	13295.0
70	9493.0
71	6608.0
72	4683.5
73	3246.5
74	2192.0
75	1496.0
76	1002.5
77	653.5
78	454.5
79	316.0
80	219.0
81	146.5
82	103.0
83	66.0
84	39.5
85	27.5
86	20.5
87	16.0
88	13.0
89	11.5
90	10.0
91	6.0
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005199584768238378
22-23	0.004839310062763333
24-25	0.0035876048863859315
26-27	0.01656186113914551
28-29	0.021180103868374243
30-31	0.03948908967989331
32-33	0.0243281746830045
34-35	0.012320271507983357
36-37	0.007296979527718735
38-39	0.0028530168944639056
40-41	0.007969302135533611
42-43	0.01001261703445328
44-45	0.006125489374897042
46-47	0.0019907786262159826
48-49	0.009796455667326771
50-51	0.00466536898091255
52-53	7.501653739566888E-6
54-55	4.924841240165945E-4
56-57	6.816920270143062E-4
58-59	5.101993093602015E-4
60-61	9.83263911779689E-4
62-63	7.736980556494169E-4
64-65	0.0
66-67	2.4267988484677678E-4
68-69	5.982578240445064E-4
70-71	3.154737290497425E-4
72-73	6.554925909168223E-4
74-75	3.401322076884675E-4
76-77	0.0
78-79	8.715116439184697E-6
80-81	4.062596845464203E-4
82-83	0.002282293172998807
84-85	3.627070751364935E-5
86-87	6.435626451233765E-4
88-89	2.7063288807379656E-4
90-91	0.008443192870226419
92-93	8.399636556415482E-4
94-95	2.954765495036486E-5
96-97	2.2274420865057507E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	558.0
22-23	1055.0
24-25	2313.0
26-27	4093.0
28-29	9007.0
30-31	21403.0
32-33	27480.0
34-35	38313.0
36-37	58741.0
38-39	64951.0
40-41	66053.0
42-43	70522.0
44-45	80336.0
46-47	79390.0
48-49	74771.0
50-51	66822.0
52-53	64847.0
54-55	69730.0
56-57	74860.0
58-59	45255.0
60-61	75183.0
62-63	73039.0
64-65	76749.0
66-67	80212.0
68-69	79368.0
70-71	74531.0
72-73	69828.0
74-75	70532.0
76-77	71044.0
78-79	75413.0
80-81	75840.0
82-83	72335.0
84-85	74203.0
86-87	79289.0
88-89	85445.0
90-91	90936.0
92-93	97512.0
94-95	120369.0
96-97	305799.0
98-99	1534760.0
100-101	3143947.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.36516414248953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.73706054901632	81.06360413363329
2	6.025084005378685	10.648150742151516
3	1.3178938248620637	3.4936771246892886
4	0.44455447170189183	1.5713251544886158
5	0.19392666426054903	0.8568180759494436
6	0.09899935402569353	0.5248856501088505
7	0.049903850147161546	0.3086833336717291
8	0.035507619785334205	0.2510109320512132
9	0.024302493820663722	0.19327444699813087
>10	0.07167658884520962	0.941106261109342
>50	7.975253948936575E-4	0.04572774674210591
>100	2.277984002048189E-4	0.04751478216578577
>500	4.3502909279625265E-5	0.031425858549240335
>1k	2.1751452060463047E-5	0.02279575769149127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3610979160229807E-5	0.0	0.0	0.0
3	0.0	1.3610979160229807E-5	0.0	0.0	0.0
4	0.0	4.0832937480689423E-5	0.0	1.3610979160229807E-5	0.0
5	0.0	5.444391664091923E-5	0.0	2.7221958320459614E-5	0.0
6	0.0	5.444391664091923E-5	0.0	2.7221958320459614E-5	0.0
7	0.0	5.444391664091923E-5	0.0	2.7221958320459614E-5	1.3610979160229807E-5
8	0.0	5.444391664091923E-5	0.0	2.7221958320459614E-5	1.3610979160229807E-5
9	0.0	1.769427290829875E-4	0.0	4.0832937480689423E-5	1.3610979160229807E-5
10-11	1.3610979160229807E-5	1.769427290829875E-4	0.0	4.0832937480689423E-5	1.3610979160229807E-5
12-13	2.7221958320459614E-5	1.9055370824321732E-4	0.0	6.805489580114903E-5	2.7221958320459614E-5
14-15	2.7221958320459614E-5	2.7902507278471106E-4	0.0	6.805489580114903E-5	4.0832937480689423E-5
16-17	4.0832937480689423E-5	2.85830562364826E-4	0.0	6.805489580114903E-5	5.444391664091923E-5
18-19	5.444391664091923E-5	3.266634998455154E-4	0.0	6.805489580114903E-5	6.124940622103413E-5
20-21	6.805489580114903E-5	3.4027447900574517E-4	0.0	1.0208234370172355E-4	6.805489580114903E-5
22-23	9.527685412160866E-5	3.879129060665495E-4	0.0	1.3610979160229807E-4	8.847136454149374E-5
24-25	1.1569332286195336E-4	4.4235682270746875E-4	0.0	2.1097017698356202E-4	1.0888783328183846E-4
26-27	1.3610979160229807E-4	4.6277329144781346E-4	0.0	3.879129060665495E-4	1.0888783328183846E-4
28-29	1.3610979160229807E-4	4.6277329144781346E-4	0.0	9.051301141552821E-4	1.0888783328183846E-4
30-31	1.633317499227577E-4	4.763842706080433E-4	0.0	0.0034299667483779113	1.1569332286195336E-4
32-33	1.633317499227577E-4	4.763842706080433E-4	0.0	0.007976033787894667	1.3610979160229807E-4
34-35	1.769427290829875E-4	5.852721038898817E-4	0.0	0.013985281087136127	1.3610979160229807E-4
36-37	1.9055370824321732E-4	7.00965426751835E-4	0.0	0.024806009519518824	1.3610979160229807E-4
38-39	1.9055370824321732E-4	7.349928746524096E-4	0.0	0.05496793933858808	1.3610979160229807E-4
40-41	1.973591978233322E-4	8.302697287740183E-4	0.0	0.08650457805284054	1.3610979160229807E-4
42-43	2.041646874034471E-4	8.642971766745928E-4	0.0	0.10545106104388044	1.3610979160229807E-4
44-45	2.041646874034471E-4	0.0010276289265973505	0.0	0.1282698676060057	1.3610979160229807E-4
46-47	2.1097017698356202E-4	0.0011569332286195336	0.0	0.1520482481989272	1.4972077076252788E-4
48-49	2.1777566656367692E-4	0.0011569332286195336	0.0	0.1737441489803335	1.633317499227577E-4
50-51	2.1777566656367692E-4	0.0013066539993820615	0.0	0.19432394947060094	1.633317499227577E-4
52-53	2.3138664572390673E-4	0.0013066539993820615	0.0	0.2476721822891217	1.9055370824321732E-4
54-55	2.3819213530402162E-4	0.001340681447282636	0.0	0.3098131076451509	2.1777566656367692E-4
56-57	2.5180311446425143E-4	0.0013610979160229807	0.0	0.37106251386618505	2.245811561437918E-4
58-59	2.7221958320459614E-4	0.0013610979160229807	0.0	0.6223348001431874	2.3138664572390673E-4
60-61	2.9263605194494084E-4	0.0013815143847633253	0.0	0.84694998373488	2.449976248841365E-4
62-63	2.9944154152505576E-4	0.001422347322244015	0.0	0.8575257145423785	2.5860860404436635E-4
64-65	2.9944154152505576E-4	0.0015788735825866577	0.0	0.8664681278506494	2.5860860404436635E-4
66-67	2.9944154152505576E-4	0.0015992900513270023	0.0	0.8748184635654505	2.5860860404436635E-4
68-69	2.9944154152505576E-4	0.001694566905448611	0.0	0.8829101906762071	2.6541409362448127E-4
70-71	2.9944154152505576E-4	0.0017149833741889556	0.0	0.8891167971732719	2.7221958320459614E-4
72-73	2.9944154152505576E-4	0.0017422053325094153	0.0	0.8939691112438938	2.85830562364826E-4
74-75	2.9944154152505576E-4	0.0017558163116696453	0.0	0.8980660159711229	2.85830562364826E-4
76-77	2.9944154152505576E-4	0.0018238712074707944	0.0	0.9017545913235452	2.85830562364826E-4
78-79	2.9944154152505576E-4	0.0018647041449514838	0.0	0.9041705401244859	2.85830562364826E-4
80-81	2.9944154152505576E-4	0.0018783151241117135	0.0	0.9051437251344425	3.198580102654005E-4
82-83	2.9944154152505576E-4	0.0018851206136918284	0.0	0.9057085807695919	3.8110741648643464E-4
84-85	3.062470311051707E-4	0.0019259535511725178	0.0	0.9058991344778351	3.8110741648643464E-4
86-87	3.130525206852856E-4	0.001959980999073092	0.0	0.9060216332902773	3.8110741648643464E-4
88	3.130525206852856E-4	0.0020144249157140115	0.0	0.9060420497590176	3.8110741648643464E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6680	0.0	40.15904	1
GTATCAA	11465	0.0	32.577736	1
TCAACGC	12450	0.0	28.740623	4
ATCAACG	12775	0.0	28.041758	3
CAACGCA	13000	0.0	27.588167	5
AACGCAG	13505	0.0	26.678785	6
TATCAAC	14100	0.0	25.962763	2
ACGCAGA	16200	0.0	22.189604	7
CGCAGAG	16750	0.0	21.48563	8
GTACATG	17945	0.0	20.813807	1
TACATGG	17705	0.0	20.326702	2
GTGGTAT	4140	0.0	19.63868	1
ACATGGG	18140	0.0	19.40699	3
TGGTATC	3865	0.0	18.686802	2
ACCGAGG	10815	0.0	17.908045	90-91
GCAGAGT	20510	0.0	17.667507	9
CATGGGG	13680	0.0	17.618671	4
GACCGAG	10650	0.0	17.608177	90-91
AGACCGA	10695	0.0	17.552408	88-89
CGAGACC	10615	0.0	17.358875	86-87
>>END_MODULE