FastQCFastQC Report
Fri 27 May 2016
SRR2140195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9521526
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93300.039.70931
GTATCAA153600.030.8306561
ATCAACG173200.026.4507373
TCAACGC174550.026.2926584
CAACGCA176850.026.0640665
AACGCAG184300.025.0765236
TATCAAC191900.024.1698742
ACGCAGA217000.021.2230267
GTACATG240400.021.1166951
TACATGG237900.021.0315722
CGCAGAG223300.020.5879158
ACATGGG243350.019.90973
CATGGGG181600.018.0545734
GCAGAGT272900.016.9055049
GTACTAG24150.016.298881
GAGTACT151200.015.90105712-13
AGAGTAC239500.015.78150910-11
CAGAGTA267900.015.33534210-11
TATACCG6900.015.2894955
ATGGGGG118050.015.1923585
Produced by FastQC (version 0.11.5)