FastQCFastQC Report
Fri 27 May 2016
SRR2140194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9028952
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.035.23921
GTATCAA200800.028.9178771
TCAACGC214150.026.1865374
ATCAACG215700.025.9980743
CAACGCA218200.025.7555665
AACGCAG224650.025.0522146
TATCAAC245850.023.25582
GTACATG258600.022.250421
TACATGG260050.021.6426332
ACGCAGA264100.021.3099177
ACATGGG262900.020.975493
CGCAGAG267750.020.9436448
CATGGGG183750.018.6823354
GCAGAGT320350.017.5301259
GAGTACT173900.017.114812-13
AGAGTAC269300.016.9657610-11
CGTCGAA3604.0910483E-516.60700494-95
CAGAGTA295650.016.50359210-11
GAGTACA187700.016.235521
ATGGGGG118350.015.6683775
Produced by FastQC (version 0.11.5)