FastQCFastQC Report
Fri 27 May 2016
SRR2140193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6446098
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91450.028.178371
TCAACGC127200.025.3714644
GTATCAA134150.025.3073331
ATCAACG128600.025.0952593
CAACGCA129100.025.0291655
AACGCAG133400.024.4059686
GTACATG158850.021.552481
TACATGG159250.021.2928642
TATCAAC158000.021.0729982
ACGCAGA156950.020.6137647
ACATGGG162150.020.2560233
CGCAGAG164400.019.6547458
CATGGGG122650.017.7085324
GAGTACT97950.016.89129612-13
GCAGAGT197250.016.4229189
CAGAGTA174950.015.81597610-11
AGAGTAC156450.015.64698710-11
GAGTACA116750.014.71471
ATGGGGG75450.014.47429855
GTACTTT114450.014.45678914-15
Produced by FastQC (version 0.11.5)