##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6446098
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46990815218757	34.0	31.0	34.0	31.0	34.0
2	32.67325737213427	34.0	31.0	34.0	31.0	34.0
3	32.779108074373056	34.0	31.0	34.0	31.0	34.0
4	36.236444900465365	37.0	37.0	37.0	35.0	37.0
5	36.08115203957495	37.0	35.0	37.0	35.0	37.0
6	36.125318293330324	37.0	35.0	37.0	35.0	37.0
7	36.109843505326786	37.0	35.0	37.0	35.0	37.0
8	36.07642313225769	37.0	35.0	37.0	35.0	37.0
9	37.945529217830696	39.0	38.0	39.0	35.0	39.0
10-11	37.831946085833636	39.0	38.0	39.0	35.0	39.0
12-13	37.85472203494269	39.0	38.0	39.0	35.0	39.0
14-15	39.218745355717516	40.0	39.0	41.0	36.0	41.0
16-17	39.1916968994266	40.0	39.0	41.0	36.0	41.0
18-19	39.21955887111862	40.0	39.0	41.0	36.0	41.0
20-21	39.217387497960786	40.0	39.0	41.0	36.0	41.0
22-23	39.18275080316286	40.0	39.0	41.0	36.0	41.0
24-25	39.187311821193376	40.0	39.0	41.0	36.0	41.0
26-27	39.06657616106307	40.0	39.0	41.0	36.0	41.0
28-29	38.98346025409798	40.0	39.0	41.0	35.5	41.0
30-31	38.89190479384713	40.0	38.0	41.0	35.0	41.0
32-33	38.81059954589469	40.0	38.0	41.0	35.0	41.0
34-35	38.75021834559924	40.0	38.0	41.0	35.0	41.0
36-37	38.72209795040963	40.0	38.0	41.0	35.0	41.0
38-39	38.6568186524845	40.0	38.0	41.0	35.0	41.0
40-41	38.633607037253796	40.0	38.0	41.0	35.0	41.0
42-43	38.60616668089029	40.0	38.0	41.0	35.0	41.0
44-45	38.54331581852925	40.0	38.0	41.0	34.5	41.0
46-47	38.46686060748153	40.0	38.0	41.0	34.0	41.0
48-49	38.42911575085382	40.0	38.0	41.0	34.0	41.0
50-51	38.353087539479745	40.0	38.0	41.0	34.0	41.0
52-53	38.18087331480125	40.0	37.5	41.0	34.0	41.0
54-55	38.03430473878613	40.0	37.0	41.0	33.5	41.0
56-57	37.881576659813405	39.5	37.0	41.0	33.0	41.0
58-59	37.71435900431378	39.0	36.0	41.0	33.0	41.0
60-61	37.57390646719893	39.0	36.0	41.0	33.0	41.0
62-63	37.361957847137106	39.0	35.5	40.5	33.0	41.0
64-65	37.128425037323694	39.0	35.0	40.0	32.0	41.0
66-67	36.88690620765675	38.0	35.0	40.0	32.0	41.0
68-69	36.60206477467713	37.0	35.0	40.0	32.0	41.0
70-71	36.29442046871915	37.0	35.0	39.5	32.0	41.0
72-73	35.97705281474572	36.0	35.0	39.0	31.5	41.0
74-75	35.58170973338137	36.0	34.5	39.0	31.0	40.0
76-77	33.61301967844106	34.5	32.0	36.5	28.0	39.0
78-79	34.743710982952784	35.0	34.0	37.0	30.5	39.0
80-81	34.72743817700841	35.0	34.0	37.0	31.0	39.0
82-83	34.47183587355022	35.0	34.0	36.0	31.0	38.5
84-85	34.17708034113839	35.0	34.0	36.0	30.5	37.0
86-87	34.0102313830185	35.0	34.0	35.5	30.0	37.0
88-89	33.835318915938615	35.0	34.0	35.0	30.0	36.5
90-91	33.679137933421096	35.0	34.0	35.0	30.0	36.0
92-93	33.61160522228056	35.0	34.0	35.0	30.0	36.0
94-95	33.5505740148182	35.0	34.0	35.0	30.0	36.0
96-97	33.57767316844626	35.0	34.0	35.0	30.0	35.5
98-99	33.61495608848378	35.0	34.0	35.0	30.0	35.0
100-101	32.35786090347686	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	31.0
21	103.0
22	415.0
23	1427.0
24	3731.0
25	8372.0
26	15598.0
27	26217.0
28	39274.0
29	56234.0
30	75806.0
31	100873.0
32	135055.0
33	185906.0
34	276789.0
35	436573.0
36	805313.0
37	1547047.0
38	1881995.0
39	834492.0
40	14838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.705121765136056	19.75590504519168	12.791738505992306	24.747234683679956
2	15.418005358386713	21.381948721449216	39.55206121033763	23.64798470982645
3	18.580232568601968	25.60397002962102	30.297134793793084	25.518662607983934
4	12.75578733833477	17.396455597542744	38.27712018308744	31.570636881035053
5	13.223764747166063	37.753722462480624	35.80365025568296	13.218862534670356
6	30.899826638690985	37.43338388088876	18.398467284355206	13.268322196065046
7	26.91757090878854	32.24089984359531	23.141565641726203	17.699963605889952
8	25.037255096028638	36.12579268884836	21.219627749996974	17.617324465126032
9	25.824925404485004	16.08779450762306	20.961626708126374	37.12565337976555
10-11	24.4669585755673	26.37304095903321	29.040765116757093	20.11923534864239
12-13	25.015720827577564	23.910892014533207	29.106556688485917	21.966830469403316
14-15	22.091195486881794	25.468649830381874	26.34662289858321	26.09353178415312
16-17	22.058049691456755	28.51694932345118	27.11289062003091	22.31211036506116
18-19	22.07901345766622	27.98487471782044	28.381754750231092	21.554357074282247
20-21	22.74190077645372	27.22985509310479	28.221673216108943	21.80657091433255
22-23	22.46351180592015	27.07617535312013	28.12633615091865	22.333976690041073
24-25	22.29453249441725	27.225876797296223	28.197917454848113	22.28167325343841
26-27	22.279236205511516	27.406705961388546	28.099463124650093	22.214594708449845
28-29	22.20466133841184	27.478617212890104	28.122224165756784	22.19449728294127
30-31	22.404860711743517	27.35514499398983	28.05950874321101	22.180485551055646
32-33	22.171090061686215	27.547222526492476	28.000770980024363	22.28091643179694
34-35	22.341155065474684	27.549881381150133	27.959289502055167	22.14967405132002
36-37	22.38106194367116	27.42842708128903	27.94569597424982	22.24481500078999
38-39	22.20600677308474	27.55156091167288	28.03976954624259	22.20266276899979
40-41	22.428738100845283	27.427618640430847	27.94441671436232	22.199226544361554
42-43	22.27953490543068	27.553934559328034	27.785408215473822	22.381122319767464
44-45	22.371138779709305	27.65852106359967	27.629331095518218	22.34100906117281
46-47	22.45878655457326	27.532703078780763	27.486010954266533	22.522499412379446
48-49	22.407773527436127	27.60622728255328	27.475211538394777	22.510787651615818
50-51	22.358946130128647	27.742906579141412	27.431097872573933	22.467049418156
52-53	22.52120318362213	27.650174578733928	27.351165665459437	22.47745657218451
54-55	22.471048199376433	27.547699197123322	27.37140958386574	22.609843019634507
56-57	22.4787328507372	27.680904177582477	27.31918831921927	22.521174652461053
58-59	22.461504458441183	27.70142127122271	27.339009548574495	22.49806472176161
60-61	22.534580497105665	27.679249801834754	27.32099956028233	22.465170140777246
62-63	22.48496414403891	27.79310447554892	27.282514035269674	22.439417345142505
64-65	22.46558337283883	27.678697574018933	27.283311566272733	22.572407486869498
66-67	22.529338763863123	27.693869077736803	27.15328521221459	22.623506946185486
68-69	22.492451452502447	27.65624643328783	27.25537808425189	22.595924029957832
70-71	22.458095526441177	27.526942166555727	27.27500095010405	22.73996135689904
72-73	22.509864963332674	27.538007630496274	27.219603131393555	22.732524274777496
74-75	22.397941271933604	27.65324390580241	27.237580117348543	22.71123470491544
76-77	22.560095330838077	27.611085262271217	27.151900659601573	22.676918747289136
78-79	22.480539450567786	27.600008130595523	27.1459329430166	22.773519475820095
80-81	22.489164567783245	27.607993324341283	27.158314117638067	22.744527990237405
82-83	22.554573944577523	27.615297376555198	27.20796323685597	22.62216544201131
84-85	22.59591298175253	27.595568673629202	27.158626039242073	22.649892305376195
86-87	22.48115973843702	27.611076479862724	27.23757281763554	22.67019096406472
88-89	22.549329454226395	27.510376866384505	27.21280549327012	22.727488186118983
90-91	22.535738348849247	27.61857839060209	27.21566029467976	22.6300229658689
92-93	22.573792431415445	27.58386681050058	27.225551916358153	22.616788841725818
94-95	22.427871675893233	27.611375438208828	27.28278815796435	22.677964727933585
96-97	22.47069106639909	27.61046864967542	27.300874374344186	22.617965909581304
98-99	22.76812916299621	28.123960105868374	27.40350155514691	21.704409175988513
100-101	26.96371665488641	35.19749188116513	27.94142116923835	9.897370294710107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1659.0
1	1413.5
2	1612.0
3	2171.5
4	2808.5
5	4242.0
6	5497.0
7	6370.5
8	6995.0
9	6957.5
10	6400.0
11	5892.5
12	6411.0
13	8478.0
14	12771.0
15	18202.0
16	22718.0
17	25463.5
18	26380.0
19	26082.0
20	25615.0
21	25643.5
22	26745.0
23	30174.0
24	36634.5
25	45857.0
26	57689.5
27	70995.5
28	85008.5
29	99985.0
30	114832.5
31	128699.5
32	141825.5
33	154876.5
34	168198.5
35	181041.0
36	194926.0
37	207460.0
38	217406.0
39	227846.5
40	238038.5
41	247209.0
42	254157.5
43	261135.0
44	270046.5
45	279164.0
46	287446.5
47	292003.5
48	291526.0
49	289185.5
50	285219.5
51	277473.0
52	266332.0
53	253200.5
54	239914.0
55	225711.0
56	210435.0
57	192997.0
58	173001.0
59	151924.5
60	129516.5
61	107418.5
62	87244.5
63	69342.0
64	53867.0
65	41744.5
66	32539.5
67	24950.5
68	18755.5
69	14283.5
70	10881.5
71	8025.0
72	5864.0
73	4283.5
74	3099.0
75	2196.0
76	1518.0
77	1004.5
78	669.5
79	460.5
80	317.5
81	224.5
82	168.5
83	115.0
84	72.5
85	50.0
86	35.0
87	27.0
88	19.0
89	12.0
90	7.5
91	5.5
92	4.5
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001985697393989356
3	0.0
4	0.003350864352357038
5	4.498845658257135E-4
6	3.568050004824624E-4
7	0.0
8	0.0
9	0.0
10-11	3.568050004824624E-4
12-13	4.886677180520681E-4
14-15	7.756630445270922E-6
16-17	0.0
18-19	0.0010781716318926582
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.434665307689788E-5
28-29	0.0
30-31	7.786202102399146E-5
32-33	9.766068287943382E-4
34-35	5.887341250786843E-4
36-37	4.426148642875643E-4
38-39	0.0016440418049504012
40-41	0.0
42-43	1.6291780576759988E-5
44-45	9.89210026764726E-5
46-47	3.3423127350220316E-5
48-49	5.080424388170841E-5
50-51	1.629091946721148E-4
52-53	0.0
54-55	0.003037416138564466
56-57	0.002650318883166295
58-59	0.001577402652398053
60-61	1.1874372427999515E-4
62-63	2.7755506553722726E-5
64-65	1.4990205774302216E-4
66-67	6.647608290251292E-5
68-69	1.3484260737444588E-4
70-71	0.0
72-73	0.0
74-75	1.304718514507717E-4
76-77	0.0
78-79	3.8176508364163447E-4
80-81	0.0
82-83	1.5938859385469838E-4
84-85	3.232939080004681E-5
86-87	0.0015960973015898003
88-89	0.0
90-91	5.641053238793794E-5
92-93	2.294977338820012E-5
94-95	2.4525736490348425E-4
96-97	2.384244909637118E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	310.0
20-21	892.0
22-23	1541.0
24-25	2389.0
26-27	4500.0
28-29	10100.0
30-31	20512.0
32-33	27596.0
34-35	38720.0
36-37	58286.0
38-39	64138.0
40-41	62469.0
42-43	69454.0
44-45	80661.0
46-47	80414.0
48-49	75238.0
50-51	68658.0
52-53	66781.0
54-55	72485.0
56-57	75414.0
58-59	73307.0
60-61	71096.0
62-63	67113.0
64-65	70175.0
66-67	74150.0
68-69	73854.0
70-71	70867.0
72-73	66499.0
74-75	67678.0
76-77	67107.0
78-79	70947.0
80-81	70623.0
82-83	66065.0
84-85	65538.0
86-87	68551.0
88-89	71823.0
90-91	74456.0
92-93	74911.0
94-95	81877.0
96-97	122473.0
98-99	505467.0
100-101	3590963.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.42208859218755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.465067058269	82.68419418167093
2	5.560798222767727	9.945163826392296
3	1.1696679401134966	3.137824504928118
4	0.3903605840820599	1.3962743493073617
5	0.16653205507706348	0.7445822091270112
6	0.08020591740295135	0.4303308390974636
7	0.05270407431145165	0.32990358815735055
8	0.0316202480925749	0.22620389009929456
9	0.019250420931991197	0.15492715614156766
>10	0.06275330653897798	0.8524384828531103
>50	8.418998311978903E-4	0.047249651630399556
>100	1.504417911656574E-4	0.02485962774117727
>500	4.78307903783968E-5	0.026047692853966898
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.102652178108369E-5
2	0.0	1.5513260890541845E-5	0.0	0.0	3.102652178108369E-5
3	0.0	1.5513260890541845E-5	0.0	1.5513260890541845E-5	3.102652178108369E-5
4	0.0	6.205304356216738E-5	0.0	6.205304356216738E-5	4.653978267162553E-5
5	0.0	6.205304356216738E-5	0.0	6.205304356216738E-5	4.653978267162553E-5
6	4.653978267162553E-5	6.205304356216738E-5	0.0	6.205304356216738E-5	4.653978267162553E-5
7	4.653978267162553E-5	6.205304356216738E-5	0.0	1.0859282623379291E-4	4.653978267162553E-5
8	4.653978267162553E-5	7.756630445270921E-5	0.0	1.0859282623379291E-4	9.307956534325106E-5
9	4.653978267162553E-5	1.0859282623379291E-4	0.0	1.5513260890541843E-4	1.2410608712433476E-4
10-11	4.653978267162553E-5	1.0859282623379291E-4	0.0	1.9391576113177305E-4	1.2410608712433476E-4
12-13	4.653978267162553E-5	1.0859282623379291E-4	0.0	2.5596880469394047E-4	1.2410608712433476E-4
14-15	4.653978267162553E-5	1.4737597846014753E-4	0.0	2.869953264750241E-4	1.5513260890541843E-4
16-17	1.0859282623379291E-4	1.5513260890541843E-4	0.0	3.568050004824624E-4	1.5513260890541843E-4
18-19	1.0859282623379291E-4	1.7064586979596028E-4	0.0	4.3437130493517164E-4	1.784025002412312E-4
20-21	1.0859282623379291E-4	2.0167239157704397E-4	0.0	5.041809789426099E-4	1.9391576113177305E-4
22-23	1.0859282623379291E-4	2.0167239157704397E-4	0.0	6.127738051764028E-4	2.0167239157704397E-4
24-25	1.0859282623379291E-4	2.404555438033986E-4	0.0	8.299594576439886E-4	2.0167239157704397E-4
26-27	1.0859282623379291E-4	2.7148206558448226E-4	0.0	0.001085928262337929	2.1718565246758582E-4
28-29	1.0859282623379291E-4	2.792386960297532E-4	0.0	0.0018383214155292087	2.2494228291285675E-4
30-31	1.0859282623379291E-4	2.9475195692029506E-4	0.0	0.0034206740263644764	2.3269891335812767E-4
32-33	1.0859282623379291E-4	2.9475195692029506E-4	0.0	0.0066396756611519095	2.482121742486695E-4
34-35	1.0859282623379291E-4	3.800748918182752E-4	0.0	0.010913579036496188	2.6372543513921137E-4
36-37	1.0859282623379291E-4	4.6539782671625534E-4	0.0	0.016676755457332482	2.6372543513921137E-4
38-39	1.0859282623379291E-4	4.6539782671625534E-4	0.0	0.025193535686239957	2.6372543513921137E-4
40-41	1.0859282623379291E-4	5.119376093878808E-4	0.0	0.03423776678542585	2.7148206558448226E-4
42-43	1.0859282623379291E-4	5.119376093878808E-4	0.0	0.04221158288316436	2.792386960297532E-4
44-45	1.1634945667906383E-4	6.205304356216737E-4	0.0	0.05141094659125567	2.9475195692029506E-4
46-47	1.2410608712433476E-4	7.136100009649248E-4	0.0	0.061735021713911264	3.0250858736556596E-4
48-49	1.2410608712433476E-4	7.136100009649248E-4	0.0	0.07169453520563913	3.4129173959192055E-4
50-51	1.3961934801487658E-4	7.98932935862905E-4	0.0	0.08134378347955616	3.4129173959192055E-4
52-53	1.5513260890541843E-4	8.066895663081759E-4	0.0	0.09027166512206299	3.6456163092773335E-4
54-55	1.5513260890541843E-4	8.066895663081759E-4	0.0	0.09905217078610967	4.2661467448990075E-4
56-57	1.5513260890541843E-4	8.066895663081759E-4	0.0	0.1085928262337929	4.809110876067972E-4
58-59	1.5513260890541843E-4	8.066895663081759E-4	0.0	0.11746641146318285	4.886677180520681E-4
60-61	1.784025002412312E-4	8.299594576439886E-4	0.0	0.1261693508227768	4.96424348497339E-4
62-63	2.3269891335812767E-4	8.687426098703433E-4	0.0	0.1341819500727417	5.274508702784227E-4
64-65	2.404555438033986E-4	9.152823925419688E-4	0.0	0.1420704432355822	5.274508702784227E-4
66-67	2.482121742486695E-4	9.152823925419688E-4	0.0	0.1499201532461964	5.274508702784227E-4
68-69	2.482121742486695E-4	9.307956534325107E-4	0.0	0.15800256217016867	5.274508702784227E-4
70-71	2.482121742486695E-4	9.463089143230525E-4	0.0	0.16539463098451188	5.584773920595064E-4
72-73	2.9475195692029506E-4	9.463089143230525E-4	0.0	0.17178609447141513	5.584773920595064E-4
74-75	2.9475195692029506E-4	9.773354361041363E-4	0.0	0.17678912110861483	5.584773920595064E-4
76-77	3.180218482561078E-4	9.92848696994678E-4	0.0	0.18083808220104627	5.584773920595064E-4
78-79	3.4129173959192055E-4	9.92848696994678E-4	0.0	0.18428978274919183	5.584773920595064E-4
80-81	3.4904837003719145E-4	9.92848696994678E-4	0.0	0.18605053786026832	5.584773920595064E-4
82-83	3.568050004824624E-4	9.92848696994678E-4	0.0	0.18664004177410892	5.584773920595064E-4
84-85	3.568050004824624E-4	0.0010083619578852199	0.0	0.18723730231839478	5.584773920595064E-4
86-87	3.568050004824624E-4	0.0010393884796663035	0.0	0.18756308079709616	5.584773920595064E-4
88-89	3.568050004824624E-4	0.001085928262337929	0.0	0.1876328904711036	5.662340225047773E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9145	0.0	28.17837	1
TCAACGC	12720	0.0	25.371464	4
GTATCAA	13415	0.0	25.307333	1
ATCAACG	12860	0.0	25.095259	3
CAACGCA	12910	0.0	25.029165	5
AACGCAG	13340	0.0	24.405968	6
GTACATG	15885	0.0	21.55248	1
TACATGG	15925	0.0	21.292864	2
TATCAAC	15800	0.0	21.072998	2
ACGCAGA	15695	0.0	20.613764	7
ACATGGG	16215	0.0	20.256023	3
CGCAGAG	16440	0.0	19.654745	8
CATGGGG	12265	0.0	17.708532	4
GAGTACT	9795	0.0	16.891296	12-13
GCAGAGT	19725	0.0	16.422918	9
CAGAGTA	17495	0.0	15.815976	10-11
AGAGTAC	15645	0.0	15.646987	10-11
GAGTACA	11675	0.0	14.7147	1
ATGGGGG	7545	0.0	14.4742985	5
GTACTTT	11445	0.0	14.456789	14-15
>>END_MODULE