FastQCFastQC Report
Fri 27 May 2016
SRR2140192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8426451
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77750.043.5133061
GTATCAA133350.031.9955581
TCAACGC153200.026.9457534
ATCAACG153700.026.831453
CAACGCA157700.026.1504145
AACGCAG163050.025.3672716
TATCAAC170050.024.587552
TACATGG200550.021.5790372
GTACATG206300.021.5696141
ACGCAGA193900.021.3106547
CGCAGAG198350.020.7709758
ACATGGG205200.020.6331023
CATGGGG152700.018.3425854
GCAGAGT245400.016.772019
GAGTACT141800.016.52257212-13
AGAGTAC213100.016.17151610-11
CAGAGTA235400.015.76367510-11
GAGTACA146100.015.131121
TATACTG42800.014.3626325
ATGGGGG95200.014.2399395
Produced by FastQC (version 0.11.5)