##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140192_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8426451 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40429286303332 33.0 31.0 34.0 31.0 34.0 2 32.60758782078007 34.0 31.0 34.0 31.0 34.0 3 32.71116547167959 34.0 31.0 34.0 31.0 34.0 4 36.189391595583956 37.0 35.0 37.0 35.0 37.0 5 36.032242518232174 37.0 35.0 37.0 35.0 37.0 6 36.075444454610846 37.0 35.0 37.0 35.0 37.0 7 36.05821501839861 37.0 35.0 37.0 35.0 37.0 8 36.01938099444238 37.0 35.0 37.0 35.0 37.0 9 37.87354379678942 39.0 38.0 39.0 35.0 39.0 10-11 37.75024188712425 39.0 37.5 39.0 35.0 39.0 12-13 37.76992995034327 39.0 37.5 39.0 35.0 39.0 14-15 39.11032758631124 40.0 38.5 41.0 36.0 41.0 16-17 39.078643784910156 40.0 38.0 41.0 36.0 41.0 18-19 39.10840702687288 40.0 38.5 41.0 36.0 41.0 20-21 39.10546100669181 40.0 39.0 41.0 36.0 41.0 22-23 39.068328213916615 40.0 39.0 41.0 36.0 41.0 24-25 39.07138725348038 40.0 39.0 41.0 36.0 41.0 26-27 38.94486270490697 40.0 38.0 41.0 35.5 41.0 28-29 38.857455769552615 40.0 38.0 41.0 35.0 41.0 30-31 38.75806037963585 40.0 38.0 41.0 35.0 41.0 32-33 38.675776222247634 40.0 38.0 41.0 35.0 41.0 34-35 38.61428970531766 40.0 38.0 41.0 35.0 41.0 36-37 38.58247106971665 40.0 38.0 41.0 35.0 41.0 38-39 38.511529196953816 40.0 38.0 41.0 34.5 41.0 40-41 38.49331832224502 40.0 38.0 41.0 34.0 41.0 42-43 38.465012704022364 40.0 38.0 41.0 34.0 41.0 44-45 38.40206223874741 40.0 38.0 41.0 34.0 41.0 46-47 38.32510641172492 40.0 38.0 41.0 34.0 41.0 48-49 38.28890182319094 40.0 38.0 41.0 34.0 41.0 50-51 38.211041068167106 40.0 37.5 41.0 34.0 41.0 52-53 38.03950039359521 40.0 37.0 41.0 33.0 41.0 54-55 37.8923532390376 40.0 37.0 41.0 33.0 41.0 56-57 37.74161523562755 39.0 36.5 41.0 33.0 41.0 58-59 37.571966055563124 39.0 36.0 41.0 33.0 41.0 60-61 37.43705305840648 39.0 36.0 40.5 33.0 41.0 62-63 37.23041647878998 39.0 35.0 40.0 32.0 41.0 64-65 37.00170796643846 38.5 35.0 40.0 32.0 41.0 66-67 36.76515256800146 38.0 35.0 40.0 32.0 41.0 68-69 36.484683675319744 37.0 35.0 40.0 31.5 41.0 70-71 36.183859430175275 37.0 35.0 39.0 31.0 41.0 72-73 35.87094761586819 36.0 34.5 39.0 31.0 41.0 74-75 35.478210428499565 36.0 34.0 39.0 31.0 40.0 76-77 33.486401577584516 34.5 32.0 36.5 28.0 39.0 78-79 34.64314228654617 35.0 34.0 37.0 30.0 39.0 80-81 34.63737284059354 35.0 34.0 37.0 31.0 39.0 82-83 34.379588770760314 35.0 34.0 36.0 30.5 38.5 84-85 34.08145270386541 35.0 34.0 36.0 30.0 37.0 86-87 33.913004814960374 35.0 34.0 35.5 30.0 37.0 88-89 33.73210584180654 35.0 34.0 35.0 30.0 36.5 90-91 33.57292600695219 35.0 33.5 35.0 30.0 36.0 92-93 33.500578572460874 35.0 33.0 35.0 30.0 36.0 94-95 33.43955316675988 35.0 33.0 35.0 30.0 36.0 96-97 33.46893986830051 35.0 33.0 35.0 30.0 35.5 98-99 33.515266719604455 35.0 33.0 35.0 30.0 35.0 100-101 32.264768176522416 34.0 31.0 34.5 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 4.0 19 11.0 20 36.0 21 139.0 22 598.0 23 2137.0 24 5655.0 25 12260.0 26 22943.0 27 37351.0 28 56186.0 29 78581.0 30 106487.0 31 142458.0 32 189285.0 33 262074.0 34 387596.0 35 605819.0 36 1098355.0 37 2018625.0 38 2342849.0 39 1038309.0 40 18693.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41608952570898 20.08302190329001 12.849229171332036 24.651659399668972 2 15.271998256887006 21.648340719727177 39.82730744107974 23.252353582306075 3 18.275499376902566 26.091233426741578 30.56846826736428 25.06479892899158 4 12.572419900486647 17.661851609134647 38.74947989640889 31.016248593969813 5 12.99754617288059 37.95954371656131 36.03291501061719 13.009995099940912 6 30.317342434234806 38.02867363095446 18.583720864547665 13.070263070263069 7 26.50205881455906 32.49080781458291 23.48807344871524 17.519059922142784 8 24.747678471043148 36.37391352539758 21.46567991672888 17.412728086830388 9 25.748230186112753 16.042910591896874 21.123804078371787 37.08505514361859 10-11 24.254947799321254 26.511486546545314 29.36750860952506 19.86605704460837 12-13 24.828642901145674 24.096005783721157 29.313649503602306 21.761701811530866 14-15 21.952613496786903 25.56087082935281 26.579870526734272 25.90664514712601 16-17 21.85961207155895 28.65461390566444 27.323151822754326 22.162622200022287 18-19 21.876261480716906 28.091639833700388 28.627773032416435 21.40432565316627 20-21 22.59774491748978 27.367532520321586 28.433686967631143 21.60103559455749 22-23 22.283406404017335 27.321446936630483 28.28671347138873 22.108433187963453 24-25 22.079494671341205 27.43238921361504 28.446616302851496 22.04149981219226 26-27 22.071192399141847 27.611607364211665 28.313969773357485 22.003230463289007 28-29 22.034550247057048 27.643038508972285 28.333915076372566 21.988496167598097 30-31 22.215125503534523 27.64151879387782 28.178769182430614 21.96458652015704 32-33 22.136692066337964 27.69064395637233 28.181413417002837 21.99125056028687 34-35 22.1504972114243 27.68684838399531 28.213371831512017 21.94928257306837 36-37 22.134335674578068 27.650052750876892 28.151830122220208 22.06378145232483 38-39 21.986221010957998 27.733832769108453 28.24070983433367 22.039236385599875 40-41 22.21269312093053 27.59738566287248 28.15815276718579 22.0317684490112 42-43 22.104488053733384 27.70846421329737 27.996109492007193 22.19093824096205 44-45 22.16027605143152 27.84580120400412 27.829022419766012 22.16490032479835 46-47 22.270035972856956 27.70843438533337 27.631983495372104 22.38954614643757 48-49 22.218719510056243 27.797331760469053 27.623236211562368 22.360712517912333 50-51 22.13550172670612 27.898599345277848 27.637617388407428 22.32828153960861 52-53 22.296906624262068 27.86236375088861 27.4853772343149 22.355352390534424 54-55 22.324368536701712 27.724278204317393 27.508578608600683 22.442774650380215 56-57 22.35649131616669 27.88362998755291 27.41369841508179 22.346180281198606 58-59 22.270379381155085 27.929139084235555 27.481552082397275 22.318929452212085 60-61 22.34372593023247 27.92041356742685 27.43712515319402 22.298735349146668 62-63 22.334046047216972 28.03237952805258 27.361906875187852 22.27166754954259 64-65 22.29329725308646 27.94458063681703 27.35008269633601 22.4120394137605 66-67 22.38321097458825 27.974715277278193 27.198028936804164 22.444044811329395 68-69 22.327343818955452 27.887569471776185 27.3402051552867 22.44488155398166 70-71 22.306107592534147 27.641925035670024 27.41358858474719 22.638378787048637 72-73 22.375093674193575 27.725961142397942 27.340867183391943 22.558078000016536 74-75 22.243318613476433 27.88822069847895 27.2977184221804 22.570742265864215 76-77 22.375698483517954 27.84793437897481 27.248059603926865 22.52830753358037 78-79 22.310543675763732 27.875242792312992 27.194593163837975 22.619620368085293 80-81 22.327134397265663 27.82574037316653 27.24552800818535 22.601597221382455 82-83 22.371395852074137 27.824876708390555 27.336985306881008 22.466742132654304 84-85 22.518143398919864 27.774142865520545 27.21936072225079 22.4883530133088 86-87 22.319509145259552 27.779612418547472 27.334024041608295 22.566854394584684 88-89 22.358928170808458 27.7638240513214 27.30612628584258 22.571121492027558 90-91 22.418467236381172 27.80552020549906 27.285520191448803 22.490492366670964 92-93 22.457269192132795 27.763061640693888 27.31303123250904 22.466637934664284 94-95 22.316253759275398 27.80945238427252 27.370630785895884 22.503663070556197 96-97 22.30313720197512 27.811777189316476 27.43606688602104 22.449018722687356 98-99 22.56624513171229 28.367735786360054 27.4755174322845 21.59050164964316 100-101 26.70415285901199 35.46289232757029 28.029371272154748 9.803583541262974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2214.0 1 1890.0 2 2279.5 3 3177.0 4 4143.5 5 6489.5 6 8460.5 7 9748.0 8 10627.5 9 10366.5 10 9402.5 11 8453.5 12 9120.5 13 12088.5 14 18396.0 15 26531.0 16 32869.0 17 36699.0 18 38197.5 19 37859.5 20 36741.5 21 36278.5 22 38301.5 23 44007.0 24 53150.5 25 66374.0 26 84000.0 27 102845.5 28 122355.5 29 143090.0 30 161141.5 31 178038.0 32 195411.5 33 212099.0 34 228532.5 35 244677.0 36 262931.0 37 279124.0 38 291401.5 39 303193.0 40 316172.0 41 328324.0 42 337860.0 43 346203.5 44 353077.5 45 360851.5 46 368996.5 47 374183.5 48 374440.5 49 371289.0 50 367675.0 51 360131.5 52 345302.0 53 327961.5 54 309837.0 55 289603.0 56 268063.0 57 244403.0 58 219213.0 59 191407.0 60 161541.0 61 133319.0 62 108023.5 63 85494.5 64 65811.5 65 50543.0 66 38991.5 67 29727.5 68 22635.5 69 17043.0 70 12591.0 71 9122.0 72 6611.5 73 4854.0 74 3469.0 75 2401.5 76 1688.5 77 1159.5 78 807.5 79 545.0 80 353.5 81 235.0 82 173.5 83 124.0 84 92.0 85 75.5 86 54.5 87 38.0 88 28.5 89 20.0 90 13.5 91 10.5 92 9.0 93 5.5 94 3.0 95 2.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0017445066730940465 3 0.0 4 0.002907511121823411 5 2.610826313474083E-4 6 3.916239470211125E-4 7 0.0 8 0.0 9 0.0 10-11 3.3228698535124694E-4 12-13 4.984304780268704E-4 14-15 1.1867392333973105E-5 16-17 0.0 18-19 0.0011096011832264852 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.125754906928437E-5 28-29 0.0 30-31 2.9776433179927134E-5 32-33 0.001063786681366844 34-35 7.566660477218467E-4 36-37 3.5074402483364453E-4 38-39 0.0016185307478699218 40-41 1.2345170726612675E-5 42-43 1.8717560128602115E-5 44-45 5.053848119987451E-5 46-47 1.2813354590688598E-5 48-49 1.9487576767248763E-5 50-51 7.897569055850226E-5 52-53 1.3322488827427808E-5 54-55 0.0031162082625385223 56-57 0.002823831027968166 58-59 0.0014491313636194392 60-61 1.6170990648948887E-4 62-63 7.1257131859113525E-6 64-65 6.49929302134801E-5 66-67 4.395506503005354E-5 68-69 1.858947246943742E-4 70-71 0.0 72-73 0.0 74-75 1.6303612391397563E-4 76-77 1.575156381525558E-5 78-79 3.6762361224084334E-4 80-81 0.0 82-83 1.3187573283756848E-4 84-85 0.0 86-87 0.0016647389103332554 88-89 0.0 90-91 1.0537681695976644E-4 92-93 0.0 94-95 1.639464459826062E-4 96-97 3.723128090312663E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 260.0 20-21 640.0 22-23 1348.0 24-25 3023.0 26-27 5645.0 28-29 13288.0 30-31 27426.0 32-33 37429.0 34-35 51123.0 36-37 77539.0 38-39 87557.0 40-41 84787.0 42-43 94854.0 44-45 109377.0 46-47 108893.0 48-49 102240.0 50-51 92738.0 52-53 91210.0 54-55 98778.0 56-57 101890.0 58-59 100045.0 60-61 96657.0 62-63 92280.0 64-65 96488.0 66-67 102044.0 68-69 99133.0 70-71 96402.0 72-73 90439.0 74-75 91773.0 76-77 90974.0 78-79 95380.0 80-81 95826.0 82-83 90169.0 84-85 89081.0 86-87 93136.0 88-89 97743.0 90-91 100543.0 92-93 102274.0 94-95 110817.0 96-97 164783.0 98-99 653937.0 100-101 4586482.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.88006793421302 #Duplication Level Percentage of deduplicated Percentage of total 1 90.8845872518387 78.96059114612657 2 6.465669859471588 11.234756732621705 3 1.5009905799141843 3.9121849065467442 4 0.5425423625234861 1.8854446925291564 5 0.2481554726610531 1.0779882161519518 6 0.12008690461947277 0.6259895058809494 7 0.06693608912926206 0.40707883795605787 8 0.04767001047386236 0.3313258998715047 9 0.028272941394775167 0.2210719562170281 >10 0.09356347758786712 1.2007387584281883 >50 0.001239290090289099 0.06931919884065665 >100 2.2381849770125654E-4 0.035983311604501564 >500 6.194179775661434E-5 0.03752683722494067 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.5602177001919315E-5 0.0 3 0.0 0.0 0.0 3.5602177001919315E-5 0.0 4 0.0 9.493913867178484E-5 0.0 4.746956933589242E-5 0.0 5 0.0 9.493913867178484E-5 0.0 5.933696166986552E-5 0.0 6 0.0 9.493913867178484E-5 0.0 5.933696166986552E-5 0.0 7 0.0 9.493913867178484E-5 0.0 7.120435400383863E-5 0.0 8 0.0 9.493913867178484E-5 0.0 7.120435400383863E-5 1.1867392333973105E-5 9 0.0 1.8987827734356967E-4 0.0 7.120435400383863E-5 1.1867392333973105E-5 10-11 0.0 1.8987827734356967E-4 0.0 8.307174633781173E-5 1.1867392333973105E-5 12-13 0.0 1.8987827734356967E-4 0.0 1.1274022717274449E-4 2.373478466794621E-5 14-15 1.7801088500959658E-5 3.026185045163142E-4 0.0 1.246076195067176E-4 3.5602177001919315E-5 16-17 4.746956933589242E-5 3.3228698535124694E-4 0.0 1.364750118406907E-4 3.5602177001919315E-5 18-19 5.933696166986552E-5 3.5602177001919314E-4 0.0 1.602097965086369E-4 5.933696166986552E-5 20-21 5.933696166986552E-5 3.7975655468713935E-4 0.0 2.2548045434548898E-4 5.933696166986552E-5 22-23 7.120435400383863E-5 4.568946048579645E-4 0.0 2.3141415051247552E-4 7.713805017082518E-5 24-25 7.713805017082518E-5 5.102978703608436E-4 0.0 2.9668480834932765E-4 9.493913867178484E-5 26-27 8.307174633781173E-5 5.340326550287897E-4 0.0 4.094250355220721E-4 1.0680653100575794E-4 28-29 8.307174633781173E-5 5.340326550287897E-4 0.0 0.001257943587401149 1.0680653100575794E-4 30-31 1.0087283483877139E-4 6.527065783685208E-4 0.0 0.00401117860888291 1.0680653100575794E-4 32-33 1.0680653100575794E-4 6.645739707024939E-4 0.0 0.009137892097159291 1.0680653100575794E-4 34-35 1.0680653100575794E-4 7.713805017082519E-4 0.0 0.015896372031356975 1.1867392333973104E-4 36-37 1.0680653100575794E-4 8.781870327140098E-4 0.0 0.025805644630224514 1.1867392333973104E-4 38-39 1.0680653100575794E-4 9.078555135489425E-4 0.0 0.0400643165194932 1.3054131567370416E-4 40-41 1.1867392333973104E-4 0.0010087283483877138 0.0 0.05381862423456803 1.3054131567370416E-4 42-43 1.1867392333973104E-4 0.0010324631330556602 0.0 0.0663624579315776 1.3054131567370416E-4 44-45 1.246076195067176E-4 0.0011214685755604583 0.0 0.08031257762016299 1.3054131567370416E-4 46-47 1.3054131567370416E-4 0.0011867392333973104 0.0 0.09431016687808427 1.3054131567370416E-4 48-49 1.3054131567370416E-4 0.001192672929564297 0.0 0.10729902778761782 1.5427610034165036E-4 50-51 1.3054131567370416E-4 0.0013232142452380012 0.0 0.12030568978565234 1.5427610034165036E-4 52-53 1.602097965086369E-4 0.0013291479414049877 0.0 0.13225615386596326 1.602097965086369E-4 54-55 1.6614349267562347E-4 0.0013410153337389608 0.0 0.14493646257481352 1.6614349267562347E-4 56-57 1.6614349267562347E-4 0.001352882726072934 0.0 0.1568513244781225 1.6614349267562347E-4 58-59 1.8987827734356967E-4 0.001352882726072934 0.0 0.16810161241072902 1.7801088500959657E-4 60-61 1.8987827734356967E-4 0.0013884849030748533 0.0 0.1788416024729747 1.8987827734356967E-4 62-63 1.8987827734356967E-4 0.001406285991575813 0.0 0.18870933919867333 2.1361306201151588E-4 64-65 1.8987827734356967E-4 0.001590230572752396 0.0 0.19813204871184797 2.1361306201151588E-4 66-67 1.8987827734356967E-4 0.0016198990535873288 0.0 0.20815999523405523 2.1954675817850244E-4 68-69 1.8987827734356967E-4 0.001643633838255275 0.0 0.2177429145437385 2.2548045434548898E-4 70-71 1.8987827734356967E-4 0.001667368622923221 0.0 0.22593734895034695 2.4328154284644865E-4 72-73 1.8987827734356967E-4 0.0016733023190902078 0.0 0.23217366362184982 2.492152390134352E-4 74-75 1.8987827734356967E-4 0.001708904496092127 0.0 0.23701555969411084 2.492152390134352E-4 76-77 2.0174566967754278E-4 0.00173857297692706 0.0 0.24129968832667514 2.551489351804218E-4 78-79 2.0174566967754278E-4 0.0017563740654280196 0.0 0.24566688870557724 2.610826313474083E-4 80-81 2.0174566967754278E-4 0.0017563740654280196 0.0 0.24764280952918377 2.6701632751439486E-4 82-83 2.0767936584452931E-4 0.001762307761595006 0.0 0.2482539802343834 2.729500236813814E-4 84-85 2.1361306201151588E-4 0.0017682414577619926 0.0 0.24879988028174613 2.729500236813814E-4 86-87 2.1361306201151588E-4 0.0017801088500959657 0.0 0.24906096291309354 2.729500236813814E-4 88-89 2.1361306201151588E-4 0.0019225175581036428 0.0 0.2491321672670974 2.729500236813814E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7775 0.0 43.513306 1 GTATCAA 13335 0.0 31.995558 1 TCAACGC 15320 0.0 26.945753 4 ATCAACG 15370 0.0 26.83145 3 CAACGCA 15770 0.0 26.150414 5 AACGCAG 16305 0.0 25.367271 6 TATCAAC 17005 0.0 24.58755 2 TACATGG 20055 0.0 21.579037 2 GTACATG 20630 0.0 21.569614 1 ACGCAGA 19390 0.0 21.310654 7 CGCAGAG 19835 0.0 20.770975 8 ACATGGG 20520 0.0 20.633102 3 CATGGGG 15270 0.0 18.342585 4 GCAGAGT 24540 0.0 16.77201 9 GAGTACT 14180 0.0 16.522572 12-13 AGAGTAC 21310 0.0 16.171516 10-11 CAGAGTA 23540 0.0 15.763675 10-11 GAGTACA 14610 0.0 15.13112 1 TATACTG 4280 0.0 14.362632 5 ATGGGGG 9520 0.0 14.239939 5 >>END_MODULE