FastQCFastQC Report
Fri 27 May 2016
SRR2140191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16387337
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158900.045.3268051
GTATCAA270250.033.279291
TCAACGC314950.027.7543774
ATCAACG315450.027.6054733
CAACGCA322700.027.0236455
AACGCAG332500.026.289296
TATCAAC342400.025.6867852
ACGCAGA396350.022.0127337
CGCAGAG411150.021.1599798
GTACATG403050.021.0619561
TACATGG396000.020.8518962
ACATGGG399650.020.143583
CATGGGG313350.018.3773614
GCAGAGT489350.017.7193579
GAGTACT288200.017.58461812-13
AGAGTAC426800.016.76379810-11
CAGAGTA473650.016.51620310-11
GAGTACA288250.015.4714021
GTGGTAT91900.015.3953941
GTACTTT341200.014.89029714-15
Produced by FastQC (version 0.11.5)