FastQCFastQC Report
Fri 27 May 2016
SRR2140190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8501376
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85050.045.5770531
GTATCAA150900.035.078231
TCAACGC176300.029.2384784
ATCAACG180950.028.4419463
CAACGCA181850.028.3444615
AACGCAG186950.027.5928426
TATCAAC188650.027.4761072
GTACATG233700.023.6779631
ACGCAGA220850.023.3576767
TACATGG235400.023.1437232
ACATGGG235000.022.437413
CGCAGAG246800.020.901718
CATGGGG188600.019.8618434
GCAGAGT274250.018.7354059
GAGTACT158250.017.64953812-13
AGAGTAC241700.017.07246410-11
CAGAGTA259650.017.06825810-11
GTGGTAT46050.016.7115141
TGGTATC41800.016.17022
ATGGGGG111000.015.8822765
Produced by FastQC (version 0.11.5)