##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8501376
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46393595577939	34.0	31.0	34.0	31.0	34.0
2	32.6779678960206	34.0	31.0	34.0	31.0	34.0
3	32.78530793132782	34.0	31.0	34.0	31.0	34.0
4	36.24772695620097	37.0	37.0	37.0	35.0	37.0
5	36.08064412161043	37.0	35.0	37.0	35.0	37.0
6	36.12549721362753	37.0	35.0	37.0	35.0	37.0
7	36.11548118798651	37.0	35.0	37.0	35.0	37.0
8	36.08716859482512	37.0	35.0	37.0	35.0	37.0
9	37.97061616848849	39.0	38.0	39.0	35.0	39.0
10-11	37.85596602244155	39.0	38.0	39.0	35.0	39.0
12-13	37.88127022025611	39.0	38.0	39.0	35.0	39.0
14-15	39.25137354235362	40.0	39.0	41.0	36.0	41.0
16-17	39.228636458380386	40.0	39.0	41.0	36.0	41.0
18-19	39.25832812241218	40.0	39.0	41.0	36.0	41.0
20-21	39.256729514789285	40.0	39.0	41.0	36.0	41.0
22-23	39.22205699174344	40.0	39.0	41.0	36.0	41.0
24-25	39.22689121654743	40.0	39.0	41.0	36.0	41.0
26-27	39.10577296065129	40.0	39.0	41.0	36.0	41.0
28-29	39.021381796181444	40.0	39.0	41.0	36.0	41.0
30-31	38.93021975064819	40.0	38.0	41.0	35.0	41.0
32-33	38.84583592133936	40.0	38.0	41.0	35.0	41.0
34-35	38.782431380404105	40.0	38.0	41.0	35.0	41.0
36-37	38.75115112096111	40.0	38.0	41.0	35.0	41.0
38-39	38.67789440501555	40.0	38.0	41.0	35.0	41.0
40-41	38.64594085712366	40.0	38.0	41.0	35.0	41.0
42-43	38.61484027055478	40.0	38.0	41.0	35.0	41.0
44-45	38.547452277179815	40.0	38.0	41.0	34.5	41.0
46-47	38.4651716127547	40.0	38.0	41.0	34.0	41.0
48-49	38.41635793268165	40.0	38.0	41.0	34.0	41.0
50-51	38.33500858598686	40.0	38.0	41.0	34.0	41.0
52-53	38.155209770472894	40.0	37.0	41.0	34.0	41.0
54-55	37.99621008701302	40.0	37.0	41.0	33.0	41.0
56-57	37.83610769145761	39.5	36.5	41.0	33.0	41.0
58-59	37.65055103193643	39.0	36.0	41.0	33.0	41.0
60-61	37.498749450983446	39.0	36.0	41.0	33.0	41.0
62-63	37.27639997147665	39.0	35.0	40.0	33.0	41.0
64-65	37.03020273239176	38.0	35.0	40.0	32.0	41.0
66-67	36.77679908142559	37.5	35.0	40.0	32.0	41.0
68-69	36.494249530923334	37.0	35.0	40.0	32.0	41.0
70-71	36.18660286835683	36.5	35.0	39.0	32.0	41.0
72-73	35.87802991837901	36.0	35.0	39.0	31.5	41.0
74-75	35.49629700762354	35.5	34.0	38.5	31.0	40.0
76-77	33.560736165201625	34.5	32.0	36.5	28.0	39.0
78-79	34.662549985270296	35.0	34.0	37.0	30.5	39.0
80-81	34.65888718046895	35.0	34.0	36.5	31.0	39.0
82-83	34.41872463629351	35.0	34.0	36.0	31.0	38.0
84-85	34.1376666790091	35.0	34.0	36.0	30.5	37.0
86-87	33.98908596945993	35.0	34.0	35.5	30.5	37.0
88-89	33.83037582835973	35.0	34.0	35.0	30.5	36.5
90-91	33.684482228588024	35.0	34.0	35.0	30.0	36.0
92-93	33.61976181966892	35.0	34.0	35.0	30.0	36.0
94-95	33.56590112725499	35.0	34.0	35.0	30.0	36.0
96-97	33.600563026590194	35.0	34.0	35.0	30.5	35.0
98-99	33.64103930843356	35.0	34.0	35.0	31.0	35.0
100-101	32.381256304457125	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	15.0
20	35.0
21	115.0
22	486.0
23	1676.0
24	4490.0
25	10009.0
26	19281.0
27	32094.0
28	48601.0
29	69987.0
30	96433.0
31	128943.0
32	174587.0
33	242800.0
34	366690.0
35	591383.0
36	1104104.0
37	2087666.0
38	2446177.0
39	1059761.0
40	16041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14332173991598	19.494538295918215	12.660762210729182	24.70137775343662
2	15.657145787817758	21.28919044592588	39.267734914472776	23.785928851783584
3	18.712853072255598	25.512081808874232	29.999872961741726	25.775192157128444
4	12.905050999342437	17.221756268901988	37.848464138128165	32.02472859362741
5	13.535302729424043	37.510095450677625	35.44376578130966	13.510836038588678
6	31.278490338060543	36.94512175541123	18.222700473230887	13.553687433297334
7	27.29514610340726	31.98929208636343	22.94113329418673	17.77442851604258
8	25.512940493397775	35.6782125622958	20.941209987653764	17.867636956652664
9	26.063004388936566	15.837447961365314	20.848448533507987	37.25109911619013
10-11	24.88430658290897	26.16887345395107	28.598499908485103	20.348320054654863
12-13	25.400898894326335	23.81166577053269	28.64152010043601	22.145915234704958
14-15	22.429068524687892	25.10427726153443	26.096934448478564	26.369719765299116
16-17	22.3773833788789	28.18113797107668	26.72209769336164	22.719380956682777
18-19	22.402853685397652	27.549338850144757	28.13654990494087	21.91125755951672
20-21	23.136372584499828	26.788109904842468	28.03573246633032	22.039785044327388
22-23	22.929017481179997	26.684906240718963	27.850420199010646	22.535656079090387
24-25	22.6793190606077	26.84988456460499	28.026990230941394	22.44380614384592
26-27	22.574898979875083	26.955457205678456	27.97809541366396	22.4915484007825
28-29	22.634845751706376	27.0167686034058	27.916855674779583	22.431529970108244
30-31	22.894358669672467	26.775683008589862	27.835043390897802	22.494914930839872
32-33	22.604097807154677	26.995956370968706	27.820347855073045	22.579597966803576
34-35	22.79781430265536	27.0223812307203	27.791226404180385	22.388578062443955
36-37	22.871964593472494	26.905971064744925	27.64399882397195	22.57806551781063
38-39	22.57910744720325	27.158348876783094	27.734563952174835	22.52797972383882
40-41	22.810316690105488	26.917107125351375	27.7076034654239	22.56497271911923
42-43	22.654473725716247	27.114131949061505	27.490956368620413	22.74043795660183
44-45	22.639008141898962	27.142144526523378	27.417507498293002	22.801339833284658
46-47	22.789098999509825	27.106668442965553	27.21761932558948	22.886613231935144
48-49	22.830800754098888	27.30532233177193	27.060963974834067	22.802912939295116
50-51	22.74322125106942	27.338352231968432	27.10145837048813	22.816968146474014
52-53	23.016350502140547	27.240767826624023	26.93014900291047	22.812732668324955
54-55	22.93542878659046	27.173533007289976	26.964569400000492	22.926468806119072
56-57	22.973709012005095	27.394159484094406	26.81460792181289	22.81752358208761
58-59	22.862503134734176	27.373976811766358	26.929827600619465	22.833692452879994
60-61	22.975297483962603	27.340711606015823	26.823966305558223	22.860024604463355
62-63	22.944594614250633	27.425516960793466	26.903744159041004	22.726144265914897
64-65	23.002705347427515	27.272285143876335	26.864938179528476	22.86007132916767
66-67	23.01407280679596	27.328871943818722	26.65582686959846	23.001228379786863
68-69	22.953592080785803	27.293460220947757	26.78394589435744	22.96900180390899
70-71	22.97119005333903	27.132396103905805	26.78248848752261	23.113925355232556
72-73	22.991242365595127	27.193928330984036	26.701412223363945	23.11341708005689
74-75	22.781451025273277	27.27516589113506	26.773294872677774	23.170088210913885
76-77	22.971275958541856	27.28771091759299	26.646026717688304	23.094986406176847
78-79	22.850947451321947	27.287874340038716	26.717052402096776	23.144125806542565
80-81	22.934204877219667	27.220863497097902	26.7166096696955	23.128321955986927
82-83	23.07214341815916	27.149261831941978	26.87540147020801	22.90319327969085
84-85	23.078392629648192	27.139014486570062	26.739015444256108	23.043577439525635
86-87	22.863048661749726	27.305847032622836	26.80419117030385	23.026913135323586
88-89	23.031629175895272	27.090791435400075	26.743790474402317	23.13378891430234
90-91	23.031137611476048	27.19576296481689	26.71655303247642	23.056546391230647
92-93	23.11315742279883	27.156098156637924	26.828582443569548	22.902161976993696
94-95	22.844619348199135	27.25345036280013	26.906740530584607	22.995189758416128
96-97	22.847692838951957	27.292373430061605	26.878484738300312	22.98144899268612
98-99	23.189789364818438	27.86935854497609	26.924398938510034	22.016453151695433
100-101	27.565253375105968	34.85849634916328	27.506762397563072	10.06948787816767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2696.0
1	2257.0
2	2537.0
3	3469.5
4	4443.5
5	6737.5
6	8885.5
7	10382.5
8	11362.5
9	11107.5
10	9984.5
11	9053.5
12	9832.5
13	13068.5
14	19918.5
15	28614.5
16	35540.0
17	39601.5
18	40603.5
19	39084.0
20	37038.5
21	35758.0
22	36208.5
23	40117.5
24	47960.5
25	59680.0
26	74937.5
27	91587.0
28	109305.5
29	127793.0
30	144070.5
31	159348.0
32	175182.5
33	190181.0
34	204006.0
35	218121.0
36	234514.0
37	248907.5
38	260914.0
39	274104.5
40	286893.5
41	299007.5
42	308499.0
43	317218.5
44	328402.5
45	343095.5
46	359426.5
47	374997.5
48	383245.0
49	383627.5
50	381901.5
51	379965.5
52	367646.0
53	349740.0
54	336619.5
55	321038.5
56	303805.5
57	282675.0
58	257180.0
59	227490.0
60	195540.0
61	166363.5
62	138545.0
63	114106.5
64	92812.0
65	73323.0
66	58894.5
67	44314.5
68	30998.5
69	23385.5
70	17406.0
71	12432.5
72	9159.0
73	6847.0
74	5222.0
75	3866.0
76	2619.5
77	1563.0
78	1136.5
79	901.0
80	602.0
81	374.5
82	275.5
83	180.0
84	102.0
85	67.5
86	49.5
87	39.0
88	32.0
89	20.5
90	11.5
91	6.5
92	2.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001940862279235738
3	0.0
4	0.0033641612840086123
5	2.587816372314317E-4
6	3.4112124907779637E-4
7	0.0
8	0.0
9	0.0
10-11	3.2935844738545857E-4
12-13	5.058004727705256E-4
14-15	2.352560338467561E-5
16-17	0.0
18-19	0.0010410079497718957
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.240287732012658E-5
28-29	0.0
30-31	2.3614409748972922E-5
32-33	0.0010250130437353512
34-35	7.918871695344692E-4
36-37	3.238084284575559E-4
38-39	0.0015021306633203073
40-41	6.121661658943374E-6
42-43	1.8562775842849024E-5
44-45	3.7595622115123436E-5
46-47	2.542411879368658E-5
48-49	0.0
50-51	2.6121270217291745E-5
52-53	6.609895158469923E-6
54-55	0.0032720590646139994
56-57	0.002949750691818253
58-59	0.0014229840801078168
60-61	1.9512933485915182E-4
62-63	7.06090674611034E-6
64-65	6.436871774724936E-5
66-67	5.0768460427580684E-5
68-69	1.6925646224852354E-4
70-71	0.0
72-73	0.0
74-75	1.9961175513625996E-4
76-77	0.0
78-79	3.5539515913884435E-4
80-81	0.0
82-83	9.765894420427127E-5
84-85	3.302364583857068E-5
86-87	0.0016253804667975403
88-89	0.0
90-91	1.125046884165346E-4
92-93	8.803948394776089E-6
94-95	2.6884300455715777E-4
96-97	9.14509377150526E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	252.0
20-21	677.0
22-23	1385.0
24-25	3135.0
26-27	5833.0
28-29	14107.0
30-31	28496.0
32-33	37965.0
34-35	52692.0
36-37	79422.0
38-39	88479.0
40-41	85718.0
42-43	96155.0
44-45	111982.0
46-47	111112.0
48-49	103524.0
50-51	93533.0
52-53	90299.0
54-55	97800.0
56-57	100591.0
58-59	99303.0
60-61	95263.0
62-63	89622.0
64-65	94666.0
66-67	100058.0
68-69	100065.0
70-71	95473.0
72-73	88858.0
74-75	90932.0
76-77	89384.0
78-79	94492.0
80-81	93914.0
82-83	88149.0
84-85	87350.0
86-87	92306.0
88-89	96222.0
90-91	98573.0
92-93	98727.0
94-95	105424.0
96-97	158395.0
98-99	663531.0
100-101	4677512.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.60120482562704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.28645088052119	79.05516630508556
2	6.142272770110606	10.638564445184404
3	1.4119122834642834	3.6681991456832757
4	0.5011036502327758	1.7358471941071196
5	0.23042477886399074	0.9977531735650139
6	0.13034634954587832	0.6772890549177231
7	0.0765467757696993	0.4640330105021152
8	0.048463923241747246	0.3357627314649616
9	0.03551561389742165	0.27681254582746495
>10	0.13283589274250893	1.852142574656078
>50	0.00341636107736419	0.19821132080872203
>100	6.880405172905009E-4	0.08772671627994441
>500	2.2680015337679006E-5	0.012491781917574628
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.352560338467561E-5
2	0.0	5.881400846168903E-5	0.0	0.0	2.352560338467561E-5
3	0.0	5.881400846168903E-5	0.0	0.0	2.352560338467561E-5
4	0.0	1.1762801692337806E-4	0.0	0.0	2.352560338467561E-5
5	0.0	1.1762801692337806E-4	0.0	0.0	2.352560338467561E-5
6	0.0	1.1762801692337806E-4	0.0	0.0	2.352560338467561E-5
7	0.0	1.1762801692337806E-4	0.0	0.0	2.352560338467561E-5
8	0.0	1.4115362030805366E-4	0.0	0.0	4.705120676935122E-5
9	0.0	3.058328440007829E-4	0.0	2.352560338467561E-5	4.705120676935122E-5
10-11	0.0	3.058328440007829E-4	0.0	7.057681015402683E-5	4.705120676935122E-5
12-13	0.0	3.058328440007829E-4	0.0	8.822101269253354E-5	4.705120676935122E-5
14-15	0.0	4.8227486938585E-4	0.0	9.410241353870244E-5	5.293260761552013E-5
16-17	0.0	4.999190719243567E-4	0.0	9.410241353870244E-5	7.645821100019573E-5
18-19	0.0	5.41088877847539E-4	0.0	1.1174661607720916E-4	9.410241353870244E-5
20-21	0.0	5.822586837707214E-4	0.0	1.2939081861571586E-4	9.998381438487134E-5
22-23	0.0	6.351912913862415E-4	0.0	1.7056062453889818E-4	1.2939081861571586E-4
24-25	0.0	6.881238990017616E-4	0.0	2.1173043046208048E-4	1.6467922369272928E-4
26-27	0.0	7.175309032326061E-4	0.0	3.058328440007829E-4	1.6467922369272928E-4
28-29	0.0	7.351751057711128E-4	0.0	9.351427345408556E-4	1.6467922369272928E-4
30-31	0.0	8.116333167713086E-4	0.0	0.002723088591776202	1.6467922369272928E-4
32-33	0.0	8.233961184636463E-4	0.0	0.005910807850399747	1.6467922369272928E-4
34-35	0.0	9.645497387717E-4	0.0	0.010851184561181625	1.6467922369272928E-4
36-37	0.0	0.0011174661607720915	0.0	0.0180617819985847	1.6467922369272928E-4
38-39	0.0	0.001205687173464625	0.0	0.027901365614225272	1.7056062453889818E-4
40-41	0.0	0.0013586035954650166	0.0	0.03852317554240631	1.8820482707740488E-4
42-43	0.0	0.001388010599695861	0.0	0.04801575650812292	1.8820482707740488E-4
44-45	0.0	0.0015174014183115768	0.0	0.059219825120074676	1.8820482707740488E-4
46-47	0.0	0.0016115038318502792	0.0	0.07127081545387476	1.8820482707740488E-4
48-49	0.0	0.0016115038318502792	0.0	0.08302185434452022	1.8820482707740488E-4
50-51	0.0	0.0018055900597738531	0.0	0.09417299034885646	1.8820482707740488E-4
52-53	0.0	0.001811471460620022	0.0	0.10411255777888191	1.9996762876974268E-4
54-55	0.0	0.0018232342623123597	0.0	0.11476377471129379	2.2349323215441828E-4
56-57	0.0	0.0018467598656970354	0.0	0.12479744455485794	2.2349323215441828E-4
58-59	0.0	0.001864404068235542	0.0	0.13408417649095863	2.2349323215441828E-4
60-61	0.0	0.0018938110724663866	0.0	0.14280041254498094	2.2349323215441828E-4
62-63	0.0	0.0019173366758510621	0.0	0.15059915006700092	2.2349323215441828E-4
64-65	0.0	0.0021525927096978184	0.0	0.15872724603640634	2.2937463300058718E-4
66-67	0.0	0.002176118313082494	0.0	0.16707295383712	2.41137434692925E-4
68-69	0.0	0.0022114067181595075	0.0	0.1751304729963714	2.470188355390939E-4
70-71	0.0	0.002229050920698014	0.0	0.18263514047608292	2.470188355390939E-4
72-73	0.0	0.002234932321544183	0.0	0.18851654132225182	2.705444389237695E-4
74-75	0.0	0.002276102127467365	0.0	0.1934039854254182	2.705444389237695E-4
76-77	0.0	0.0023290347350828854	0.0	0.19789737567189125	2.705444389237695E-4
78-79	0.0	0.002340797536775223	0.0	0.20186143984220906	2.705444389237695E-4
80-81	0.0	0.002340797536775223	0.0	0.2033729598596745	2.7642583976993844E-4
82-83	0.0	0.002340797536775223	0.0	0.20413754196967643	3.058328440007829E-4
84-85	0.0	0.002340797536775223	0.0	0.20481978446783203	3.058328440007829E-4
86-87	0.0	0.0023819673426984057	0.0	0.20506680330337113	3.117142448469518E-4
88-89	0.0	0.0024937139587756147	0.0	0.20514326151437132	3.2935844738545857E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8505	0.0	45.577053	1
GTATCAA	15090	0.0	35.07823	1
TCAACGC	17630	0.0	29.238478	4
ATCAACG	18095	0.0	28.441946	3
CAACGCA	18185	0.0	28.344461	5
AACGCAG	18695	0.0	27.592842	6
TATCAAC	18865	0.0	27.476107	2
GTACATG	23370	0.0	23.677963	1
ACGCAGA	22085	0.0	23.357676	7
TACATGG	23540	0.0	23.143723	2
ACATGGG	23500	0.0	22.43741	3
CGCAGAG	24680	0.0	20.90171	8
CATGGGG	18860	0.0	19.861843	4
GCAGAGT	27425	0.0	18.735405	9
GAGTACT	15825	0.0	17.649538	12-13
AGAGTAC	24170	0.0	17.072464	10-11
CAGAGTA	25965	0.0	17.068258	10-11
GTGGTAT	4605	0.0	16.711514	1
TGGTATC	4180	0.0	16.1702	2
ATGGGGG	11100	0.0	15.882276	5
>>END_MODULE