FastQCFastQC Report
Fri 27 May 2016
SRR2140189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424725
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.041.1377941
GTATCAA94550.031.683931
TCAACGC111000.026.3721754
ATCAACG112100.026.0775243
CAACGCA112150.025.9579325
AACGCAG116250.025.0768186
TATCAAC121850.024.387272
GTACATG155150.022.7555351
TACATGG156100.022.1533722
ACATGGG157400.021.689053
ACGCAGA138800.020.9451277
CGCAGAG144350.020.1398228
CATGGGG125400.019.6241764
GCAGAGT174950.016.5482759
AGAGTAC153650.016.47393410-11
GAGTACT97150.015.9150512-13
ATGGGGG78800.015.512375
CAGAGTA170600.015.08464510-11
GAGTACA111450.014.7205631
GTGGTAT31100.014.48111251
Produced by FastQC (version 0.11.5)