##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6424725
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48529828124939	34.0	31.0	34.0	31.0	34.0
2	32.69074909821043	34.0	31.0	34.0	31.0	34.0
3	32.800162964173566	34.0	31.0	34.0	31.0	34.0
4	36.25703216869204	37.0	37.0	37.0	35.0	37.0
5	36.097301440917704	37.0	35.0	37.0	35.0	37.0
6	36.14167049951554	37.0	36.0	37.0	35.0	37.0
7	36.12918965403188	37.0	35.0	37.0	35.0	37.0
8	36.09953562214725	37.0	35.0	37.0	35.0	37.0
9	37.977134274229634	39.0	38.0	39.0	35.0	39.0
10-11	37.86030149150353	39.0	38.0	39.0	35.0	39.0
12-13	37.886827451758634	39.0	38.0	39.0	35.0	39.0
14-15	39.261571973897716	40.0	39.0	41.0	36.0	41.0
16-17	39.23645315558254	40.0	39.0	41.0	36.0	41.0
18-19	39.26436835817876	40.0	39.0	41.0	36.0	41.0
20-21	39.26154887728814	40.0	39.0	41.0	36.0	41.0
22-23	39.225307415406995	40.0	39.0	41.0	36.0	41.0
24-25	39.23467587077032	40.0	39.0	41.0	36.0	41.0
26-27	39.1138924751953	40.0	39.0	41.0	36.0	41.0
28-29	39.03258564493404	40.0	39.0	41.0	36.0	41.0
30-31	38.942259862641336	40.0	38.0	41.0	35.0	41.0
32-33	38.866321617915276	40.0	38.0	41.0	35.0	41.0
34-35	38.80941535649518	40.0	38.0	41.0	35.0	41.0
36-37	38.78415788186274	40.0	38.0	41.0	35.0	41.0
38-39	38.72208686774977	40.0	38.0	41.0	35.0	41.0
40-41	38.702040035157424	40.0	38.0	41.0	35.0	41.0
42-43	38.674534083908064	40.0	38.0	41.0	35.0	41.0
44-45	38.61465770157774	40.0	38.0	41.0	35.0	41.0
46-47	38.53893924321078	40.0	38.0	41.0	34.0	41.0
48-49	38.50255115803145	40.0	38.0	41.0	34.0	41.0
50-51	38.428531282944554	40.0	38.0	41.0	34.0	41.0
52-53	38.25707076843607	40.0	37.5	41.0	34.0	41.0
54-55	38.108188398400074	40.0	37.0	41.0	33.5	41.0
56-57	37.961028149930186	40.0	37.0	41.0	33.0	41.0
58-59	37.788929467119395	39.0	36.5	41.0	33.0	41.0
60-61	37.647575639763005	39.0	36.0	41.0	33.0	41.0
62-63	37.433637388904785	39.0	35.5	40.5	33.0	41.0
64-65	37.19363258213846	39.0	35.0	40.0	33.0	41.0
66-67	36.950900810201595	38.0	35.0	40.0	32.5	41.0
68-69	36.66517168920889	37.0	35.0	40.0	32.0	41.0
70-71	36.35531805171383	37.0	35.0	39.5	32.0	41.0
72-73	36.03958800302358	36.5	35.0	39.0	32.0	41.0
74-75	35.64074225959489	36.0	34.5	39.0	31.0	40.0
76-77	33.68378130979545	34.5	32.0	36.5	29.0	39.0
78-79	34.79697945258599	35.0	34.0	37.0	30.5	39.0
80-81	34.77471273203723	35.0	34.0	37.0	31.0	39.0
82-83	34.52081586250141	35.0	34.0	36.0	31.0	38.5
84-85	34.22593254849267	35.0	34.0	36.0	31.0	37.0
86-87	34.06571781462297	35.0	34.0	35.5	31.0	37.0
88-89	33.89353200483233	35.0	34.0	35.0	31.0	36.5
90-91	33.74093997921922	35.0	34.0	35.0	30.0	36.0
92-93	33.67548373372218	35.0	34.0	35.0	30.0	36.0
94-95	33.616183951078845	35.0	34.0	35.0	30.0	36.0
96-97	33.637870482658045	35.0	34.0	35.0	31.0	35.5
98-99	33.67301095118728	35.0	34.0	35.0	31.0	35.0
100-101	32.42057949861892	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	3.0
20	32.0
21	80.0
22	362.0
23	1254.0
24	3274.0
25	7463.0
26	14051.0
27	23253.0
28	35211.0
29	51601.0
30	69279.0
31	93500.0
32	126131.0
33	175205.0
34	263487.0
35	419981.0
36	783106.0
37	1529283.0
38	1911790.0
39	899577.0
40	16798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65175552260992	19.864040873344774	12.790710886458175	24.693492717587134
2	15.354178304869578	21.52178119021518	39.71440725598142	23.409633248933826
3	18.312363564199245	25.86467125052045	30.472572756032356	25.350392429247943
4	12.701138126949274	17.523799117223543	38.64333163203442	31.131731123792765
5	13.18270028770179	37.83129132094408	35.843667914557365	13.142340476796765
6	30.565948164156953	37.66513793240312	18.63978190438316	13.129131999056767
7	26.80237675542533	32.37844421356556	23.400254485600552	17.418924545408558
8	24.949472545517512	36.09606636859943	21.413974917214357	17.540486168668696
9	25.92300837779049	16.061138803606383	21.06222445318671	36.95362836541642
10-11	24.369916497320343	26.269747213256828	29.377734756340523	19.982601533082303
12-13	25.007778574529326	23.919229523437597	29.23821022587984	21.834781676153234
14-15	22.140036231912255	25.422761417107008	26.44191819631757	25.995284154663167
16-17	22.07768425885933	28.513780745479377	27.14728645973174	22.261248535929553
18-19	22.098179993957647	27.94488850674955	28.49522207361056	21.461709425682244
20-21	22.78487317666823	27.244860800547162	28.23890412101795	21.73136190176666
22-23	22.556181233587107	27.14269602516425	28.07678947077975	22.224333270468893
24-25	22.29373555743681	27.250385096092238	28.18478321966647	22.27109612680448
26-27	22.2966684857873	27.396151434432564	28.060077244218384	22.247102835561748
28-29	22.27280491192253	27.54594636902674	28.025840932235614	22.155407786815115
30-31	22.439972515645128	27.395813620819975	27.941672160776914	22.222541702757983
32-33	22.258924668020807	27.533839610573345	27.946522475946573	22.260713245459268
34-35	22.348201580924798	27.527817925970393	27.96029848067111	22.163682012433696
36-37	22.399370843218886	27.447123550392632	27.891210373265352	22.262295233123126
38-39	22.24824610078515	27.6050812442252	27.9557726769154	22.19089997807425
40-41	22.439495493854295	27.428132028462947	27.852782839276397	22.279589638406357
42-43	22.279385738270598	27.57029462418527	27.696900372945443	22.45341926459869
44-45	22.416111929015383	27.61504370256212	27.56607233896326	22.402772029459236
46-47	22.45523629366296	27.572986030309664	27.416706446744605	22.55507122928277
48-49	22.429500875692884	27.626932474654435	27.41403695107539	22.529529698577292
50-51	22.421476599502945	27.718790245447572	27.370414618973644	22.489318536075835
52-53	22.581146962927846	27.634155734041265	27.18004863147845	22.60464867155244
54-55	22.549771683932487	27.527199965157088	27.244997470124982	22.678030880785442
56-57	22.55058026646526	27.632873362242062	27.226492195626147	22.590054175666534
58-59	22.52826464876636	27.654539929422985	27.234387187068066	22.582808234742593
60-61	22.57855488551444	27.621042265073864	27.23767740406928	22.562725445342416
62-63	22.596184197815955	27.701066084944515	27.158848634344885	22.543901082894646
64-65	22.58526171072536	27.653845063362237	27.109793734002245	22.65109949191016
66-67	22.613289205702646	27.65558906470312	27.034221760927462	22.69689996866677
68-69	22.55754024350136	27.60280305310827	27.131780940695265	22.707875762695103
70-71	22.543251066912543	27.453090311951016	27.14207719528891	22.861581425847533
72-73	22.604554156580768	27.481828961631198	27.115206158745835	22.7984107230422
74-75	22.447385156242188	27.63829550493247	27.1443606622415	22.769958676583844
76-77	22.63320909245189	27.543524160950838	27.02444429114772	22.79882245544955
78-79	22.55027472610158	27.554808706446167	26.971378523508978	22.923538043943278
80-81	22.59610877083709	27.540712572461985	27.0365902298568	22.826588426844125
82-83	22.611997362572524	27.522795353222453	27.13865555179373	22.726551732411302
84-85	22.76328483441613	27.490699069703815	27.00096970747609	22.74504638840396
86-87	22.5956626362308	27.511727719626638	27.1072045474878	22.785405096654763
88-89	22.631959769864807	27.41638859694988	27.08321835013926	22.868433283046052
90-91	22.65835761848419	27.48534544753368	27.059457819279984	22.79683911470215
92-93	22.718031599935077	27.437360951897844	27.108250940846013	22.73635650732107
94-95	22.622981361095313	27.48000330656415	27.133603698416675	22.763411633923866
96-97	22.59635768930716	27.451073617272108	27.148615487601653	22.80395320581908
98-99	22.892574043699078	28.018242675311413	27.222970451006788	21.86621282998272
100-101	27.181648439950195	35.02284726791514	27.771373855598124	10.02413043653654
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1160.0
1	1089.0
2	1532.0
3	2138.5
4	2747.5
5	4361.0
6	5794.0
7	6674.0
8	7122.5
9	6823.0
10	6242.0
11	5721.0
12	6166.0
13	8040.5
14	12127.5
15	17565.5
16	21987.0
17	24755.5
18	26078.0
19	26094.0
20	25682.0
21	25796.5
22	27170.0
23	31305.0
24	38526.0
25	48554.5
26	61243.0
27	75732.5
28	91705.0
29	108347.5
30	123020.0
31	136251.0
32	149756.5
33	162311.5
34	174049.5
35	185875.5
36	199211.0
37	212601.0
38	223885.5
39	232993.5
40	242249.5
41	252850.5
42	260823.5
43	267640.5
44	275179.0
45	282095.0
46	286413.5
47	288853.5
48	290549.0
49	288939.0
50	285638.5
51	280828.5
52	271691.5
53	258611.5
54	244875.5
55	230611.0
56	214346.5
57	196173.5
58	176785.5
59	155419.0
60	131346.5
61	108170.5
62	88105.5
63	70096.0
64	53937.0
65	41702.0
66	32624.5
67	25191.5
68	19305.0
69	14493.5
70	10676.5
71	7781.5
72	5764.5
73	4164.0
74	2895.5
75	2019.0
76	1407.0
77	976.0
78	699.5
79	492.0
80	336.5
81	232.5
82	161.5
83	115.0
84	81.0
85	55.0
86	42.0
87	30.5
88	17.5
89	10.5
90	8.0
91	4.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018989139612979543
3	0.0
4	0.003455400814820868
5	2.646027650988953E-4
6	3.112973707045827E-4
7	0.0
8	0.0
9	0.0
10-11	3.268622392398118E-4
12-13	4.046865819159575E-4
14-15	3.112973707045827E-5
16-17	0.0
18-19	0.0011829300086774143
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.673063519472617E-5
28-29	0.0
30-31	7.032039456304587E-5
32-33	9.413476851201407E-4
34-35	6.309400043881878E-4
36-37	3.5769094754955073E-4
38-39	0.0015431374427512083
40-41	0.0
42-43	0.0
44-45	3.337059438284465E-5
46-47	2.541406285473796E-5
48-49	1.7197422106426247E-5
50-51	3.4884644764865725E-5
52-53	8.838550788787626E-6
54-55	0.0029303750942850427
56-57	0.0027285367839500916
58-59	0.0014496730756374775
60-61	1.312089234064161E-4
62-63	0.0
64-65	1.0608097623815064E-4
66-67	4.895814617042037E-5
68-69	1.5916916877283953E-4
70-71	0.0
72-73	0.0
74-75	1.5627528078240158E-4
76-77	2.116058620748759E-5
78-79	2.9022549553584816E-4
80-81	0.0
82-83	1.4440072311439037E-4
84-85	2.257232907837406E-5
86-87	0.0013189926337128783
88-89	0.0
90-91	1.425682554433451E-4
92-93	0.0
94-95	2.3442004296302495E-4
96-97	1.2611353522344735E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	172.0
20-21	490.0
22-23	995.0
24-25	2433.0
26-27	4689.0
28-29	11144.0
30-31	23834.0
32-33	31564.0
34-35	43559.0
36-37	65853.0
38-39	74218.0
40-41	71602.0
42-43	78825.0
44-45	90205.0
46-47	89068.0
48-49	83498.0
50-51	77039.0
52-53	75849.0
54-55	81545.0
56-57	82717.0
58-59	80717.0
60-61	77279.0
62-63	73873.0
64-65	77059.0
66-67	80504.0
68-69	79307.0
70-71	76625.0
72-73	72847.0
74-75	73353.0
76-77	73345.0
78-79	75927.0
80-81	75383.0
82-83	71512.0
84-85	70235.0
86-87	73385.0
88-89	76326.0
90-91	77671.0
92-93	77394.0
94-95	83374.0
96-97	120874.0
98-99	480809.0
100-101	3387627.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.98861007237477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.50149900673897	80.51089717141743
2	6.1689709354459525	10.85598356373534
3	1.3618047619355884	3.59469924577961
4	0.4557371354575763	1.603987084291109
5	0.2042209952913304	0.898456076164058
6	0.10378410430595979	0.547909145129266
7	0.06336960441356136	0.39030623892298427
8	0.03819552536900686	0.26886169505624347
9	0.02389459085986021	0.18922066542064536
>10	0.0771641478206923	1.028088405538611
>50	0.0011210500245074	0.06178579191217207
>100	2.124717590630207E-4	0.036907634155959684
>500	2.5670577935285955E-5	0.012897282476720992
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.225947414091654E-5
2	0.0	1.5564868535229135E-5	0.0	0.0	6.225947414091654E-5
3	0.0	1.5564868535229135E-5	0.0	0.0	6.225947414091654E-5
4	0.0	6.225947414091654E-5	0.0	0.0	6.225947414091654E-5
5	0.0	6.225947414091654E-5	0.0	0.0	6.225947414091654E-5
6	0.0	6.225947414091654E-5	0.0	1.5564868535229135E-5	6.225947414091654E-5
7	0.0	6.225947414091654E-5	0.0	1.5564868535229135E-5	6.225947414091654E-5
8	0.0	6.225947414091654E-5	0.0	1.5564868535229135E-5	7.782434267614567E-5
9	1.5564868535229135E-5	1.2451894828183308E-4	0.0	1.5564868535229135E-5	1.400838168170622E-4
10-11	1.5564868535229135E-5	1.2451894828183308E-4	0.0	1.5564868535229135E-5	1.5564868535229134E-4
12-13	1.5564868535229135E-5	1.2451894828183308E-4	0.0	1.5564868535229135E-5	1.712135538875205E-4
14-15	1.5564868535229135E-5	2.0234329095797875E-4	0.0	1.5564868535229135E-5	2.0234329095797875E-4
16-17	1.5564868535229135E-5	2.0234329095797875E-4	0.0	1.5564868535229135E-5	2.1790815949320787E-4
18-19	1.5564868535229135E-5	2.4903789656366616E-4	0.0	4.66946056056874E-5	2.4903789656366616E-4
20-21	1.5564868535229135E-5	2.9573250216935357E-4	0.0	6.225947414091654E-5	2.568203308312807E-4
22-23	1.5564868535229135E-5	3.346446735074264E-4	0.0	8.560677694376023E-5	2.646027650988953E-4
24-25	1.5564868535229135E-5	3.8133927911311377E-4	0.0	1.634311196199059E-4	2.646027650988953E-4
26-27	1.5564868535229135E-5	4.202514504511866E-4	0.0	2.2569059376082243E-4	2.646027650988953E-4
28-29	1.5564868535229135E-5	4.202514504511866E-4	0.0	8.09373163831915E-4	2.646027650988953E-4
30-31	1.5564868535229135E-5	4.66946056056874E-4	0.0	0.0027705465992707862	2.646027650988953E-4
32-33	1.5564868535229135E-5	4.66946056056874E-4	0.0	0.0064672028763877055	2.646027650988953E-4
34-35	1.5564868535229135E-5	6.225947414091654E-4	0.0	0.011191140476829748	2.9573250216935357E-4
36-37	1.5564868535229135E-5	7.938082952966859E-4	0.0	0.017712820393090755	2.9573250216935357E-4
38-39	1.5564868535229135E-5	8.249380323671441E-4	0.0	0.027721030861243088	2.9573250216935357E-4
40-41	1.5564868535229135E-5	8.560677694376024E-4	0.0	0.037449073695761295	2.9573250216935357E-4
42-43	1.5564868535229135E-5	8.716326379728315E-4	0.0	0.046865819159574926	3.112973707045827E-4
44-45	1.5564868535229135E-5	9.805867177194354E-4	0.0	0.05790131095105238	3.268622392398118E-4
46-47	1.5564868535229135E-5	0.0010739759289308103	0.0	0.06941153123285432	3.268622392398118E-4
48-49	1.5564868535229135E-5	0.0010739759289308103	0.0	0.08047815276140224	3.268622392398118E-4
50-51	1.5564868535229135E-5	0.0012374070485507162	0.0	0.0909299619828086	3.42427107775041E-4
52-53	1.5564868535229135E-5	0.0012451894828183307	0.0	0.10138177120421496	3.7355684484549926E-4
54-55	1.5564868535229135E-5	0.0012529719170859452	0.0	0.11232387378448105	4.046865819159575E-4
56-57	1.5564868535229135E-5	0.001276319219888789	0.0	0.12310254524512722	4.202514504511866E-4
58-59	1.5564868535229135E-5	0.001276319219888789	0.0	0.13364774367774496	4.3581631898641573E-4
60-61	1.5564868535229135E-5	0.0012918840884240182	0.0	0.14311118374716428	4.3581631898641573E-4
62-63	1.5564868535229135E-5	0.0013230138254944764	0.0	0.1521465899318648	4.3581631898641573E-4
64-65	1.5564868535229135E-5	0.001385273299635393	0.0	0.16125982045924145	4.3581631898641573E-4
66-67	1.5564868535229135E-5	0.0014086206024382366	0.0	0.1702485320383363	4.5916362178925947E-4
68-69	1.5564868535229135E-5	0.0014164030367058511	0.0	0.17918276657755777	4.747284903244886E-4
70-71	1.5564868535229135E-5	0.0014164030367058511	0.0	0.18787574565448328	4.980757931273323E-4
72-73	1.5564868535229135E-5	0.0014164030367058511	0.0	0.19469315807291362	5.136406616625614E-4
74-75	1.5564868535229135E-5	0.0014397503395086949	0.0	0.200117514757441	5.136406616625614E-4
76-77	1.5564868535229135E-5	0.001494227379381997	0.0	0.20527726867686946	5.136406616625614E-4
78-79	1.5564868535229135E-5	0.001509792247917226	0.0	0.21020354956826948	5.292055301977906E-4
80-81	1.5564868535229135E-5	0.001509792247917226	0.0	0.21218028787224355	5.292055301977906E-4
82-83	1.5564868535229135E-5	0.001509792247917226	0.0	0.21288848939059649	5.447703987330197E-4
84-85	1.5564868535229135E-5	0.001525357116452455	0.0	0.21352664900054086	5.447703987330197E-4
86-87	1.5564868535229135E-5	0.0015564868535229135	0.0	0.21384572880551309	5.447703987330197E-4
88-89	1.5564868535229135E-5	0.0015876165905933718	0.0	0.213907988279654	5.447703987330197E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5640	0.0	41.137794	1
GTATCAA	9455	0.0	31.68393	1
TCAACGC	11100	0.0	26.372175	4
ATCAACG	11210	0.0	26.077524	3
CAACGCA	11215	0.0	25.957932	5
AACGCAG	11625	0.0	25.076818	6
TATCAAC	12185	0.0	24.38727	2
GTACATG	15515	0.0	22.755535	1
TACATGG	15610	0.0	22.153372	2
ACATGGG	15740	0.0	21.68905	3
ACGCAGA	13880	0.0	20.945127	7
CGCAGAG	14435	0.0	20.139822	8
CATGGGG	12540	0.0	19.624176	4
GCAGAGT	17495	0.0	16.548275	9
AGAGTAC	15365	0.0	16.473934	10-11
GAGTACT	9715	0.0	15.91505	12-13
ATGGGGG	7880	0.0	15.51237	5
CAGAGTA	17060	0.0	15.084645	10-11
GAGTACA	11145	0.0	14.720563	1
GTGGTAT	3110	0.0	14.4811125	1
>>END_MODULE