FastQCFastQC Report
Fri 27 May 2016
SRR2140188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9679098
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84950.040.9146421
GTATCAA146350.033.2378771
TCAACGC168650.028.004614
ATCAACG172650.027.3088873
CAACGCA173850.027.0266725
AACGCAG178000.026.5778396
TATCAAC181950.026.2465152
GTACATG267600.024.25711
TACATGG277500.023.0716762
ACATGGG281150.022.1398183
ACGCAGA215500.021.8594957
CGCAGAG228500.020.5981838
CTTATAC74300.020.4285721
CATGGGG230050.019.916074
TATACAC93750.018.2136443
GCAGAGT276200.016.9385539
GAGTACA194100.016.4301131
GAGTACT127200.016.27988212-13
GTACTAG20350.016.2662871
AGAGTAC232300.016.02996610-11
Produced by FastQC (version 0.11.5)