##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140188_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9679098 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47513435652785 34.0 31.0 34.0 31.0 34.0 2 32.67362144695714 34.0 31.0 34.0 31.0 34.0 3 32.780181996297586 34.0 31.0 34.0 31.0 34.0 4 36.23824844009225 37.0 37.0 37.0 35.0 37.0 5 36.080159328896144 37.0 35.0 37.0 35.0 37.0 6 36.12720772121534 37.0 35.0 37.0 35.0 37.0 7 36.11198347201361 37.0 35.0 37.0 35.0 37.0 8 36.07794765586628 37.0 35.0 37.0 35.0 37.0 9 37.94958238877218 39.0 38.0 39.0 35.0 39.0 10-11 37.831713192696256 39.0 38.0 39.0 35.0 39.0 12-13 37.853722216677625 39.0 38.0 39.0 35.0 39.0 14-15 39.2199385727885 40.0 39.0 41.0 36.0 41.0 16-17 39.19023642492307 40.0 39.0 41.0 36.0 41.0 18-19 39.21187609630567 40.0 39.0 41.0 36.0 41.0 20-21 39.20517964663573 40.0 39.0 41.0 36.0 41.0 22-23 39.16947491775511 40.0 39.0 41.0 36.0 41.0 24-25 39.17403726620559 40.0 39.0 41.0 36.0 41.0 26-27 39.04589935771513 40.0 39.0 41.0 36.0 41.0 28-29 38.96226156638521 40.0 38.5 41.0 35.0 41.0 30-31 38.86963473577801 40.0 38.0 41.0 35.0 41.0 32-33 38.795956913500326 40.0 38.0 41.0 35.0 41.0 34-35 38.73693967081482 40.0 38.0 41.0 35.0 41.0 36-37 38.70188224519672 40.0 38.0 41.0 35.0 41.0 38-39 38.63016735569461 40.0 38.0 41.0 35.0 41.0 40-41 38.60517722403051 40.0 38.0 41.0 35.0 41.0 42-43 38.57030699672798 40.0 38.0 41.0 35.0 41.0 44-45 38.49968601145278 40.0 38.0 41.0 34.0 41.0 46-47 38.4127543498021 40.0 38.0 41.0 34.0 41.0 48-49 38.36385265769474 40.0 38.0 41.0 34.0 41.0 50-51 38.263537906164416 40.0 37.5 41.0 34.0 41.0 52-53 38.07332731825534 40.0 37.0 41.0 33.5 41.0 54-55 37.903703414761466 39.5 37.0 41.0 33.0 41.0 56-57 37.726624294217416 39.0 36.0 41.0 33.0 41.0 58-59 37.53446472895125 39.0 36.0 41.0 33.0 41.0 60-61 37.36309619884449 39.0 35.5 40.5 33.0 41.0 62-63 37.123687701238964 38.5 35.0 40.0 32.5 41.0 64-65 36.86343616227593 38.0 35.0 40.0 32.0 41.0 66-67 36.60094343108679 37.0 35.0 40.0 32.0 41.0 68-69 36.30207011931493 37.0 35.0 39.5 32.0 41.0 70-71 35.982376953057084 36.0 35.0 39.0 31.5 41.0 72-73 35.66569118302841 36.0 34.5 39.0 31.0 40.0 74-75 35.274215591722935 35.0 34.0 37.5 31.0 40.0 76-77 33.36148761243004 34.5 32.0 36.0 28.0 38.5 78-79 34.48434381403063 35.0 34.0 36.5 30.5 39.0 80-81 34.48987676179416 35.0 34.0 36.0 31.0 38.5 82-83 34.26651961516899 35.0 34.0 36.0 31.0 37.0 84-85 34.003103851745976 35.0 34.0 35.5 30.5 37.0 86-87 33.869024562753566 35.0 34.0 35.0 30.0 36.5 88-89 33.724195784368476 35.0 34.0 35.0 30.0 36.0 90-91 33.590328971559586 35.0 34.0 35.0 30.0 36.0 92-93 33.543683511898934 35.0 34.0 35.0 30.0 36.0 94-95 33.49935603047115 35.0 33.5 35.0 30.0 35.0 96-97 33.53402413167253 35.0 34.0 35.0 30.0 35.0 98-99 33.57941560407839 35.0 33.5 35.0 30.0 35.0 100-101 32.29823452403293 34.0 31.0 34.5 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 4.0 19 15.0 20 47.0 21 149.0 22 669.0 23 2086.0 24 5658.0 25 12401.0 26 23480.0 27 39016.0 28 58569.0 29 83123.0 30 112598.0 31 151369.0 32 202904.0 33 285062.0 34 431799.0 35 691378.0 36 1298312.0 37 2429236.0 38 2671251.0 39 1159429.0 40 20543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20062675261682 19.25434580784284 12.448195069416593 25.096832370123746 2 15.7610010701605 20.997049051536937 39.03011099789832 24.211838880404244 3 18.726435045910268 25.51347243307176 29.831385114604686 25.92870740641328 4 13.203942517200753 16.96987774609453 37.208490192628396 32.61768954407632 5 13.733258326088315 37.44834857499149 35.154624956026225 13.663768142893968 6 31.666760856185828 36.4442254609871 18.09073126573047 13.798282417096608 7 27.655975794438696 31.656906459672175 22.696959985320948 17.990157760568184 8 25.828832397399015 35.4102107448442 20.877575575740632 17.883381282016156 9 26.39383339232643 15.67590285789027 20.316996480457167 37.61326726932613 10-11 25.247834980865676 25.64826595195624 28.438884866177506 20.66501420100058 12-13 25.766219546201192 23.238641159610033 28.470556037180106 22.524583257008672 14-15 22.877299400966848 24.50382509856033 25.511109145822175 27.107766354650646 16-17 22.956627776679188 27.69883102743665 26.175946353678825 23.168594842205337 18-19 23.13889078876651 27.12839960714893 27.42141583839464 22.311293765689918 20-21 23.635524308132798 26.367466784919024 27.203102760525756 22.793906146422422 22-23 23.531037973124462 26.305475656784434 27.089744221637176 23.07374214845393 24-25 23.283225213172027 26.503740991170808 27.18295109516719 23.03008270048997 26-27 23.2412380493005 26.582052121153843 27.148251487229754 23.028458342315904 28-29 23.216370440994407 26.53547787007295 27.184489581541083 23.063662107391565 30-31 23.338798786874694 26.657316581598938 26.925511749635966 23.078372881890395 32-33 23.27503847583472 26.69408349205369 27.007404011129527 23.023474020982064 34-35 23.27777726904657 26.569023414862595 27.110462842199862 23.042736473890972 36-37 23.235832873581295 26.687114301067627 26.989591438724876 23.087461386626202 38-39 23.18121190749881 26.632710785164953 27.0635655380118 23.122511769324436 40-41 23.33438136844355 26.471825550334305 27.04774545443963 23.14604762678252 42-43 23.225430134531035 26.562267360117858 26.934229483625945 23.278073021725167 44-45 23.196560458315428 26.657497383169922 26.841715151591135 23.30422700692352 46-47 23.44847748927511 26.573467682316405 26.597273374073495 23.38078145433499 48-49 23.263638105772863 26.713540216151337 26.537981849645703 23.484839828430097 50-51 23.186261861560233 26.777346123400118 26.606811515302866 23.429580499736787 52-53 23.43148251709451 26.659188837573318 26.54675273665475 23.362575908677428 54-55 23.33220221305832 26.571151509240725 26.506977102566964 23.58966917513399 56-57 23.360058243407547 26.73758638752444 26.45613621408006 23.44621915498795 58-59 23.28883433041317 26.843273671000205 26.506750088335995 23.36114191025063 60-61 23.412847751391837 26.84643651248783 26.383118393680466 23.35759734243987 62-63 23.4465110077568 27.003534089928706 26.27058925028039 23.2793656520341 64-65 23.39737434993167 26.92898132183515 26.223409709473472 23.450234618759712 66-67 23.43154589051352 26.801774015839268 26.2258334545596 23.540846639087608 68-69 23.326631108503694 26.72108498572522 26.395265733607374 23.557018172163712 70-71 23.376077872655262 26.59422943718701 26.339826388270776 23.68986630188695 72-73 23.478292473791598 26.661689383558006 26.224820101401487 23.635198041248913 74-75 23.232133660204777 26.811506424122562 26.34129224092348 23.61506767474918 76-77 23.53656043566418 26.65423745281456 26.247982123009074 23.56121998851219 78-79 23.37024860313493 26.64156854775424 26.226397363940368 23.761785485170467 80-81 23.439167498483748 26.727620094400095 26.267992627670623 23.565219779445535 82-83 23.4777794408739 26.645488123703963 26.353121669166057 23.523610766256077 84-85 23.48863285261932 26.68170837507636 26.23983377218377 23.589825000120545 86-87 23.375438860845325 26.66494387101635 26.3864537030061 23.573163565132223 88-89 23.3877706725195 26.631065746108668 26.319428132610533 23.661735448761302 90-91 23.457790986865533 26.685382947115855 26.30795804845133 23.548868017567283 92-93 23.50982963053084 26.62973427423 26.34036069398549 23.520075401253667 94-95 23.36605032041083 26.59594675952226 26.433139484342544 23.60486343572436 96-97 23.430767821130498 26.577448821963067 26.388949362757813 23.602833994148625 98-99 23.71791497041027 27.164788522101464 26.45960615494024 22.65769035254803 100-101 28.43579666098144 34.149602024718 26.83110235841607 10.583498955884492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1386.0 1 1366.0 2 2060.5 3 2902.0 4 3777.0 5 6159.0 6 8297.5 7 9664.5 8 10464.5 9 10081.5 10 8883.0 11 7703.0 12 8112.5 13 10522.5 14 16006.0 15 23384.0 16 29475.5 17 33145.5 18 34139.0 19 33080.0 20 31417.0 21 30554.0 22 31633.5 23 35560.0 24 42767.5 25 53651.0 26 67751.5 27 83237.0 28 101058.0 29 120565.5 30 138394.5 31 156723.5 32 175371.0 33 192570.5 34 209092.0 35 226079.5 36 246432.0 37 267869.0 38 288685.5 39 306403.5 40 321564.0 41 338897.0 42 354984.5 43 369021.5 44 385487.5 45 405418.5 46 425466.5 47 442873.5 48 452917.5 49 457162.0 50 460685.0 51 462073.5 52 457799.5 53 446174.5 54 431565.5 55 413434.0 56 390845.5 57 363655.5 58 332082.5 59 293820.5 60 250362.5 61 209155.5 62 172495.0 63 138918.5 64 107763.0 65 82983.5 66 64196.5 67 49821.5 68 38225.0 69 28168.0 70 20581.0 71 15024.0 72 10768.0 73 7566.0 74 5167.5 75 3590.5 76 2528.0 77 1705.5 78 1165.5 79 795.0 80 542.0 81 375.5 82 259.0 83 173.5 84 112.0 85 75.0 86 54.0 87 42.5 88 27.5 89 16.5 90 13.0 91 10.5 92 9.0 93 6.5 94 4.0 95 2.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0018183512554578948 3 0.0 4 0.003171783155827124 5 3.409408603983553E-4 6 3.202777779499701E-4 7 0.0 8 0.0 9 0.0 10-11 2.737858424411035E-4 12-13 5.269086024338218E-4 14-15 1.0331541224192585E-5 16-17 0.0 18-19 0.0011571326171095696 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.237751449825012E-5 28-29 0.0 30-31 2.5932112943894886E-5 32-33 0.0010100797103882793 34-35 7.06404630665496E-4 36-37 3.689246153143981E-4 38-39 0.0016033083762315979 40-41 1.6167834190884242E-5 42-43 2.1807322168238803E-5 44-45 3.313665440297497E-5 46-47 3.362384710833234E-5 48-49 1.7054433316995187E-5 50-51 1.7293842268052824E-5 52-53 2.3362126570278036E-5 54-55 0.003065411623236057 56-57 0.002725283063266186 58-59 0.0015108272705297058 60-61 1.545896014006065E-4 62-63 1.8823378234201476E-5 64-65 7.637841558705246E-5 66-67 4.523768071322503E-5 68-69 1.641304895165589E-4 70-71 0.0 72-73 0.0 74-75 1.3047372468629481E-4 76-77 6.969912699055479E-6 78-79 3.8918608947020243E-4 80-81 0.0 82-83 1.5340394590012614E-4 84-85 1.483646836564506E-5 86-87 0.0016122696432077416 88-89 0.0 90-91 1.0131783326357991E-4 92-93 7.93630511982154E-6 94-95 1.1321210311778855E-4 96-97 2.4786247532322506E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 248.0 20-21 726.0 22-23 1383.0 24-25 3991.0 26-27 6784.0 28-29 17228.0 30-31 35174.0 32-33 44700.0 34-35 60092.0 36-37 93986.0 38-39 110755.0 40-41 105916.0 42-43 113082.0 44-45 130117.0 46-47 128430.0 48-49 123533.0 50-51 115143.0 52-53 109597.0 54-55 118278.0 56-57 122451.0 58-59 120960.0 60-61 119719.0 62-63 112838.0 64-65 115187.0 66-67 123256.0 68-69 117457.0 70-71 113739.0 72-73 106623.0 74-75 107958.0 76-77 105582.0 78-79 111444.0 80-81 111548.0 82-83 105261.0 84-85 103038.0 86-87 106561.0 88-89 112166.0 90-91 115257.0 92-93 115910.0 94-95 124112.0 96-97 183098.0 98-99 741638.0 100-101 5164132.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.82894535161903 #Duplication Level Percentage of deduplicated Percentage of total 1 89.32435069140124 74.87966112678328 2 7.218274303952008 12.102006443179773 3 1.877781562173255 4.722373438730988 4 0.702322837739239 2.3549993113614667 5 0.332440018025373 1.3934048051870116 6 0.17121216137419887 0.8611520951622176 7 0.11114174902670546 0.6521826923810135 8 0.06975214709486612 0.4677799141578899 9 0.041766885082522444 0.3151146534381113 >10 0.14601261266400978 1.9213192386889164 >50 0.003916116509938585 0.21281417760012833 >100 0.0010289149568965161 0.11719210332914184 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0331541224192585E-5 2 0.0 1.0331541224192585E-5 0.0 0.0 1.0331541224192585E-5 3 0.0 1.0331541224192585E-5 0.0 0.0 1.0331541224192585E-5 4 0.0 1.0331541224192585E-4 0.0 0.0 2.066308244838517E-5 5 0.0 1.1364695346611844E-4 0.0 0.0 2.066308244838517E-5 6 0.0 1.1364695346611844E-4 0.0 0.0 5.165770612096293E-5 7 0.0 1.2397849469031102E-4 0.0 0.0 5.165770612096293E-5 8 0.0 1.2397849469031102E-4 0.0 0.0 8.265232979354068E-5 9 0.0 2.789516130531998E-4 0.0 0.0 9.298387101773327E-5 10-11 0.0 2.789516130531998E-4 0.0 0.0 9.814964162982956E-5 12-13 0.0 2.789516130531998E-4 0.0 0.0 1.0331541224192585E-4 14-15 0.0 4.442562726402812E-4 0.0 0.0 1.1881272407821473E-4 16-17 0.0 4.5458781386447376E-4 0.0 0.0 1.291442653024073E-4 18-19 0.0 4.700851257007626E-4 0.0 1.0331541224192585E-5 1.4980734775079247E-4 20-21 0.0 4.855824375370515E-4 0.0 6.198924734515551E-5 1.9113351264756282E-4 22-23 0.0 5.165770612096292E-4 0.0 1.0331541224192585E-4 2.066308244838517E-4 24-25 0.0 5.734005379426884E-4 0.0 1.446415771386962E-4 2.2729390693223688E-4 26-27 0.0 6.147267028394588E-4 0.0 3.719354840709331E-4 2.2729390693223688E-4 28-29 0.0 6.198924734515551E-4 0.0 0.0013999238358780952 2.2729390693223688E-4 30-31 0.0 7.542025093660587E-4 0.0 0.004225600360694767 2.2729390693223688E-4 32-33 0.0 7.696998212023475E-4 0.0 0.009412034055239445 2.3762544815642946E-4 34-35 0.0 8.83346774668466E-4 0.0 0.016633781370950063 2.4795698938062205E-4 36-37 0.0 9.969937281345846E-4 0.0 0.026391922057199958 2.4795698938062205E-4 38-39 0.0 0.00105898297547974 0.0 0.040747598588215556 2.582885306048146E-4 40-41 0.0 0.0011674641583337621 0.0 0.05571800182207061 2.582885306048146E-4 42-43 0.0 0.0012036245526184362 0.0 0.06823982978579202 2.634543012169109E-4 44-45 0.0 0.0013689292122055175 0.0 0.08184130380744156 2.686200718290072E-4 46-47 0.0 0.00151873655995631 0.0 0.09643460578661359 2.789516130531998E-4 48-49 0.0 0.00151873655995631 0.0 0.11013422944989296 2.789516130531998E-4 50-51 0.0 0.0016840412195433913 0.0 0.12311581099809094 2.789516130531998E-4 52-53 0.0 0.0016840412195433913 0.0 0.1353173611838624 2.9961469550158495E-4 54-55 0.0 0.0016943727607675839 0.0 0.14748791674596123 3.202777779499701E-4 56-57 0.0 0.0017047043019917764 0.0 0.15902824829338436 3.3060931917416273E-4 58-59 0.0 0.0017047043019917764 0.0 0.17005200277959787 3.4610663101045157E-4 60-61 0.0 0.0017563620081127392 0.0 0.18136504042008872 3.512724016225479E-4 62-63 0.0 0.0018131854848457986 0.0 0.19153127698469424 3.512724016225479E-4 64-65 0.0 0.001942329750148206 0.0 0.20116027340564174 3.512724016225479E-4 66-67 0.0 0.0019629928325965913 0.0 0.21070661749679567 3.512724016225479E-4 68-69 0.0 0.0019836559150449764 0.0 0.220702383631202 3.512724016225479E-4 70-71 0.0 0.002014650538717554 0.0 0.2294790279011536 3.616039428467405E-4 72-73 0.0 0.002040479391778036 0.0 0.2359878988723949 4.029301077435108E-4 74-75 0.0 0.002102468639123191 0.0 0.24143778686815653 4.029301077435108E-4 76-77 0.0 0.002190286739528828 0.0 0.2461644669782246 4.029301077435108E-4 78-79 0.0 0.002241944445649791 0.0 0.25019893382627184 4.029301077435108E-4 80-81 0.0 0.002241944445649791 0.0 0.25204827970540233 4.0809587835560714E-4 82-83 0.0 0.002247110216261887 0.0 0.25276632182048364 4.184274195797997E-4 84-85 0.0 0.002272939069322369 0.0 0.25342237468821993 4.2359319019189597E-4 86-87 0.0 0.0023194310048312355 0.0 0.2536238397420917 4.2359319019189597E-4 88-89 0.0 0.0023969175640126796 0.0 0.2537013263012731 4.2359319019189597E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8495 0.0 40.914642 1 GTATCAA 14635 0.0 33.237877 1 TCAACGC 16865 0.0 28.00461 4 ATCAACG 17265 0.0 27.308887 3 CAACGCA 17385 0.0 27.026672 5 AACGCAG 17800 0.0 26.577839 6 TATCAAC 18195 0.0 26.246515 2 GTACATG 26760 0.0 24.2571 1 TACATGG 27750 0.0 23.071676 2 ACATGGG 28115 0.0 22.139818 3 ACGCAGA 21550 0.0 21.859495 7 CGCAGAG 22850 0.0 20.598183 8 CTTATAC 7430 0.0 20.428572 1 CATGGGG 23005 0.0 19.91607 4 TATACAC 9375 0.0 18.213644 3 GCAGAGT 27620 0.0 16.938553 9 GAGTACA 19410 0.0 16.430113 1 GAGTACT 12720 0.0 16.279882 12-13 GTACTAG 2035 0.0 16.266287 1 AGAGTAC 23230 0.0 16.029966 10-11 >>END_MODULE