FastQCFastQC Report
Fri 27 May 2016
SRR2140187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12925525
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152650.044.7515561
GTATCAA259750.032.7538071
TCAACGC319800.025.8308034
ATCAACG320750.025.7933963
CAACGCA325350.025.3130725
AACGCAG333150.024.8584526
TATCAAC339350.024.6273612
ACGCAGA385050.021.4426967
CGCAGAG397250.020.8052148
GTACATG379900.020.01781
TACATGG381450.019.4870932
ACATGGG383250.018.8927483
AGAGTAC419150.018.02262910-11
GCAGAGT462150.017.8382979
CATGGGG268800.017.3876914
GAGTACT243200.016.91456812-13
CAGAGTA458250.016.23397610-11
GTGGTAT78200.015.8782481
AGTACTT279750.015.23514412-13
TATACCG11050.014.75474555
Produced by FastQC (version 0.11.5)