##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12925525
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8601774396011	31.0	31.0	34.0	30.0	34.0
2	32.00526137236205	33.0	31.0	34.0	30.0	34.0
3	32.07207397765274	33.0	31.0	34.0	30.0	34.0
4	35.77165260211868	37.0	35.0	37.0	35.0	37.0
5	35.633671282210976	37.0	35.0	37.0	33.0	37.0
6	35.63390160167575	37.0	35.0	37.0	33.0	37.0
7	35.51508592494309	37.0	35.0	37.0	33.0	37.0
8	35.51016736264098	37.0	35.0	37.0	33.0	37.0
9	37.11571715655651	39.0	37.0	39.0	33.0	39.0
10-11	36.98673817891343	39.0	37.0	39.0	33.0	39.0
12-13	36.94807348250845	39.0	37.0	39.0	33.0	39.0
14-15	38.10883198167966	40.0	37.0	41.0	33.0	41.0
16-17	38.107299316662186	40.0	37.0	41.0	33.0	41.0
18-19	38.13738997062015	40.0	37.0	41.0	33.0	41.0
20-21	38.12467196712088	40.0	37.0	41.0	33.0	41.0
22-23	38.037776999279515	40.0	37.0	41.0	33.0	41.0
24-25	37.94157528060303	40.0	37.0	41.0	33.0	41.0
26-27	37.77679339224366	40.0	37.0	41.0	32.0	41.0
28-29	37.64684194599277	40.0	37.0	41.0	32.0	41.0
30-31	37.510465322692994	39.0	36.5	41.0	32.0	41.0
32-33	37.65947789807768	39.5	37.0	41.0	32.0	41.0
34-35	37.62305608335106	40.0	37.0	41.0	32.0	41.0
36-37	37.49359447606483	39.5	36.0	41.0	31.5	41.0
38-39	37.33667882392404	39.0	36.0	41.0	31.0	41.0
40-41	37.27640856475294	39.0	36.0	41.0	31.0	41.0
42-43	37.16707490315912	39.0	36.0	40.0	31.0	41.0
44-45	37.23118152226522	39.0	36.0	40.5	31.0	41.0
46-47	37.38637612503107	39.0	36.0	41.0	31.0	41.0
48-49	37.31574828330802	39.0	36.0	41.0	31.0	41.0
50-51	37.17836098017638	39.0	36.0	41.0	31.0	41.0
52-53	37.04215712666662	39.0	35.5	41.0	31.0	41.0
54-55	36.91277201213639	39.0	35.0	40.0	31.0	41.0
56-57	36.76433107457502	39.0	35.0	40.0	30.0	41.0
58-59	36.63023987659716	38.5	35.0	40.0	30.0	41.0
60-61	36.396848075318545	38.0	35.0	40.0	30.0	41.0
62-63	36.137896268485015	38.0	34.0	40.0	29.0	41.0
64-65	35.91672142631863	37.5	34.0	40.0	29.0	41.0
66-67	35.664504537257095	37.0	34.0	40.0	29.0	41.0
68-69	35.38561160120959	36.5	34.0	39.0	29.0	41.0
70-71	35.081758029955665	36.0	34.0	39.0	29.0	40.5
72-73	34.75970118852994	36.0	33.0	39.0	28.0	40.0
74-75	34.423622500658595	35.0	33.0	38.0	28.0	40.0
76-77	33.293195215579615	34.5	31.5	36.5	26.5	39.0
78-79	33.67777611249187	35.0	32.0	37.0	28.0	39.0
80-81	33.557526260099564	35.0	32.0	36.0	28.0	39.0
82-83	33.31907150377757	35.0	32.0	36.0	27.5	37.5
84-85	33.10129966329774	35.0	32.0	35.5	27.0	37.0
86-87	32.88222987375553	34.0	32.0	35.0	27.0	37.0
88-89	32.65216859628551	34.0	32.0	35.0	27.0	36.0
90-91	32.51551214154095	34.0	32.0	35.0	27.0	36.0
92-93	32.43952547506152	34.0	31.0	35.0	27.0	36.0
94-95	32.40189604868297	34.0	31.0	35.0	27.0	35.0
96-97	32.30446793462647	34.0	31.0	35.0	27.0	35.0
98-99	32.462637398979126	34.0	31.0	35.0	27.0	35.0
100-101	31.712872543527283	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	0.0
17	0.0
18	3.0
19	24.0
20	115.0
21	639.0
22	3060.0
23	10711.0
24	27318.0
25	56974.0
26	100927.0
27	156881.0
28	221391.0
29	291116.0
30	363370.0
31	446418.0
32	547490.0
33	683272.0
34	884148.0
35	1178810.0
36	1694207.0
37	2480148.0
38	2562777.0
39	1172571.0
40	43153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.03327911245384	22.346426934302475	15.602058717150754	24.018235236092924
2	13.784783077787349	22.638173515642585	41.238179658742304	22.338863747827766
3	16.63906108262527	27.71581038294383	31.980426326977046	23.664702207453857
4	11.833376207155997	19.37267538455885	39.82779036054628	28.966158047738872
5	12.221205715048326	38.84137007974531	36.6504648747343	12.286959330472069
6	28.835223327485732	39.86587005170003	18.8905518344516	12.408354786362642
7	25.140479787087948	33.39592782498196	24.425352161711032	17.038240226219052
8	23.803706232435434	37.85191704012023	21.429257225528556	16.915119501915783
9	25.22682831064889	16.385848930701073	21.55243984286905	36.83488291578099
10-11	23.542188808578373	27.218403120956403	29.98885925329919	19.25054881716603
12-13	24.263741704882396	24.876668452538677	29.923442181265365	20.936147661313566
14-15	21.505288953446765	26.31707029308287	27.173971656857265	25.003669096613095
16-17	21.156649343063435	29.4376321271283	27.843936706632803	21.561781823175462
18-19	21.2098282369848	28.74059732641162	29.382150224217412	20.667424212386162
20-21	22.0427878109684	27.81880456588286	29.197836187193875	20.940571435954862
22-23	21.772361510801108	27.737500976924462	29.03396292407227	21.456174588202156
24-25	21.5502583154982	27.936286008393296	29.0600320519655	21.453423624143007
26-27	21.558368864044116	28.018791512800483	28.949582634730564	21.473256988424836
28-29	21.539370939624003	28.07914763843312	28.94385048229176	21.437630939651118
30-31	21.809700547650714	27.968252375220043	28.819092224982366	21.40295485214687
32-33	21.635726516283214	28.109359376381022	28.75598650729544	21.49892760004033
34-35	21.7190338733799	28.085212891876843	28.835784829728606	21.35996840501465
36-37	21.708802744413557	28.04957369698777	28.76683510753553	21.474788451063137
38-39	21.59279632836575	28.11874093877317	28.82503799972672	21.46342473313436
40-41	21.737747612417504	27.96539129714451	28.731620093256026	21.565240997181956
42-43	21.662321099135546	28.129689737479712	28.491176947709373	21.71681221567537
44-45	21.702327297854882	28.16867261039001	28.37156321517162	21.757436876583487
46-47	21.793890297897548	28.110298518353943	28.187524220983228	21.908286962765285
48-49	21.768841990378192	28.238244079011054	28.14082350305187	21.852090427558878
50-51	21.762842941612142	28.29530269707797	28.03117872883693	21.91067563247296
52-53	21.84826428364005	28.203125564221814	27.959029035452993	21.989581116685144
54-55	21.81680661370082	28.1268823554153	28.00262492549997	22.053686105383903
56-57	21.8798353099912	28.157603762510252	27.968306474806298	21.99425445269225
58-59	21.798347014877972	28.17930098427444	28.01534022182276	22.007011779024825
60-61	21.9360200180675	28.141774723170514	27.980899025335475	21.941306233426513
62-63	21.910260884932296	28.23040933088869	27.921314807042346	21.93801497713667
64-65	21.804708813719248	28.161492505423453	27.91691954055241	22.116879140304896
66-67	21.881710733795025	28.252254584904286	27.748980507888938	22.117054173411752
68-69	21.84634637919428	28.218646742470128	27.747915233680086	22.187091644655503
70-71	21.853049010579078	28.030470638969824	27.81299976649107	22.303480583960027
72-73	21.877900138912253	28.05440551665687	27.73988507624346	22.32780926818742
74-75	21.811580620984422	28.108000986576403	27.74451968986355	22.33589870257563
76-77	21.98123949072045	28.040642911116382	27.669080357496988	22.30903724066618
78-79	21.90625926400639	28.047754896309968	27.616106258988786	22.429879580694852
80-81	22.000201448159583	28.03765372719295	27.585888501489862	22.3762563231576
82-83	21.984705482282603	28.005687663687723	27.732380915493337	22.27722593853634
84-85	22.07669451972169	28.028334203434973	27.68154207703709	22.21342919980624
86-87	21.942877918525163	28.036592314835097	27.697625505779033	22.322904260860703
88-89	21.962109157430653	27.977562756002488	27.74047682647676	22.3198512600901
90-91	22.004571350483594	27.97136689950189	27.79761912558214	22.226442624432373
92-93	22.096606745761076	27.894208893086496	27.7779983811621	22.23118597999032
94-95	21.899680902762682	27.91956308877317	27.912486312401942	22.268269696062205
96-97	21.792767914609783	27.928114890528576	28.048143755476158	22.230973439385483
98-99	21.90767643501633	28.52813570944389	28.155645621700177	21.4085422338396
100-101	22.239039804945225	31.382780778924523	25.432630210845826	20.94554920528443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2220.0
1	2071.0
2	2848.0
3	3991.5
4	5280.5
5	8126.0
6	10560.0
7	12594.5
8	14236.5
9	14453.0
10	13679.5
11	12915.0
12	14331.0
13	18873.0
14	29600.5
15	44645.0
16	56937.5
17	63250.0
18	64757.0
19	63499.5
20	60534.5
21	57849.0
22	58088.0
23	63969.5
24	76807.5
25	97267.5
26	124715.0
27	156895.0
28	196478.5
29	243152.0
30	290243.0
31	336231.5
32	377063.0
33	412069.5
34	443721.5
35	467656.0
36	488611.0
37	505543.5
38	513018.0
39	513714.5
40	511868.0
41	510417.0
42	506014.0
43	502688.5
44	502003.5
45	501810.0
46	501480.5
47	500029.5
48	497265.0
49	491760.5
50	483239.0
51	469050.0
52	446439.0
53	418973.0
54	392064.5
55	363831.5
56	335918.0
57	305279.0
58	270254.5
59	232707.0
60	194006.0
61	158643.0
62	127025.5
63	99438.0
64	75196.0
65	57137.5
66	44339.0
67	33668.0
68	25292.0
69	18749.5
70	13431.0
71	9407.0
72	6542.5
73	4510.5
74	3127.5
75	2150.0
76	1434.5
77	912.0
78	613.0
79	431.0
80	299.0
81	209.5
82	168.5
83	117.5
84	82.5
85	64.5
86	48.0
87	37.5
88	25.0
89	14.0
90	10.0
91	9.0
92	7.5
93	3.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003512429862616799
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.670787066676208E-5
20-21	0.0018336667009028486
22-23	2.9791271134343663E-4
24-25	4.2569784647973425E-5
26-27	3.406941201343992E-4
28-29	0.003470621594912534
30-31	3.0247855582156435E-4
32-33	5.4414813764522534E-5
34-35	0.0022845802606183663
36-37	0.055847131195801786
38-39	0.03335804109535634
40-41	0.04224705927838387
42-43	7.960976334137618E-4
44-45	0.0024906067392508322
46-47	0.0013623320808443752
48-49	5.769374765850955E-4
50-51	0.0
52-53	9.657284796435774E-4
54-55	0.0025032145764853193
56-57	0.0011375498314181085
58-59	0.003949144012233533
60-61	0.00141546242414716
62-63	9.277863666741612E-5
64-65	7.997773134005594E-4
66-67	3.5735416783522987E-4
68-69	1.3291009260121137E-4
70-71	0.0011661372511952094
72-73	0.0017287773502468993
74-75	6.446609656352728E-4
76-77	0.001046043898297607
78-79	1.3760839364021184E-4
80-81	0.00221717897233455
82-83	0.0024720857357911925
84-85	0.004070455089260592
86-87	0.003930519127118289
88-89	0.07004522145586389
90-91	7.040017917664207E-4
92-93	6.14338161212163E-5
94-95	4.0143772052192636E-5
96-97	0.00794739431273288
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	365.0
20-21	1346.0
22-23	2637.0
24-25	4887.0
26-27	6169.0
28-29	10267.0
30-31	27587.0
32-33	35825.0
34-35	50803.0
36-37	79466.0
38-39	87518.0
40-41	95001.0
42-43	106643.0
44-45	125841.0
46-47	127772.0
48-49	119488.0
50-51	110848.0
52-53	100686.0
54-55	111418.0
56-57	117757.0
58-59	125640.0
60-61	130002.0
62-63	124331.0
64-65	134174.0
66-67	143035.0
68-69	146522.0
70-71	149336.0
72-73	143306.0
74-75	146306.0
76-77	146677.0
78-79	160478.0
80-81	163677.0
82-83	157986.0
84-85	161703.0
86-87	172068.0
88-89	186090.0
90-91	205832.0
92-93	222300.0
94-95	291624.0
96-97	365159.0
98-99	1112972.0
100-101	7013983.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.52934242789964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84555998722806	75.94586105786799
2	6.936715582418908	11.7271201358247
3	1.7369876981496093	4.404792837898125
4	0.6440772810883599	2.1777371617259416
5	0.2996976098000789	1.266662094180696
6	0.1758575897736374	0.891907585471254
7	0.0972017011991466	0.5751477119665932
8	0.06929748087104198	0.4686136391951306
9	0.04326863550883736	0.32917223765830417
>10	0.14731011212449055	1.9167665466188744
>50	0.0032206935557552202	0.18206129343366378
>100	7.739208226542156E-4	0.09743384572362902
>500	3.170745932670582E-5	0.01672385243519916
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.736629653340967E-6	7.736629653340967E-6
2	0.0	1.5473259306681934E-5	0.0	7.736629653340967E-6	7.736629653340967E-6
3	0.0	1.5473259306681934E-5	0.0	1.5473259306681934E-5	7.736629653340967E-6
4	0.0	8.510292618675064E-5	0.0	3.094651861336387E-5	7.736629653340967E-6
5	0.0	8.510292618675064E-5	0.0	3.868314826670483E-5	7.736629653340967E-6
6	4.64197779200458E-5	8.510292618675064E-5	0.0	3.868314826670483E-5	7.736629653340967E-6
7	4.64197779200458E-5	8.510292618675064E-5	0.0	3.868314826670483E-5	7.736629653340967E-6
8	5.4156407573386764E-5	1.0831281514677353E-4	0.0	3.868314826670483E-5	7.736629653340967E-6
9	5.4156407573386764E-5	2.32098889600229E-4	0.0	3.868314826670483E-5	7.736629653340967E-6
10-11	6.96296668800687E-5	2.32098889600229E-4	0.0	4.255146309337531E-5	7.736629653340967E-6
12-13	8.510292618675064E-5	2.3983551925356997E-4	0.0	4.64197779200458E-5	7.736629653340967E-6
14-15	8.897124101342112E-5	3.3654338992033205E-4	0.0	5.4156407573386764E-5	7.736629653340967E-6
16-17	1.663375375468308E-4	3.4041170474700255E-4	0.0	7.736629653340966E-5	7.736629653340967E-6
18-19	1.7407416720017176E-4	3.6362159370702544E-4	0.0	1.2765438928012595E-4	7.736629653340967E-6
20-21	1.9728405616019465E-4	3.945681123203893E-4	0.0	1.7020585237350125E-4	1.5473259306681934E-5
22-23	2.3983551925356997E-4	4.758027236804695E-4	0.0	3.6362159370702544E-4	3.094651861336387E-5
24-25	2.6304540821359287E-4	5.144858719471743E-4	0.0	0.001168231077654486	4.255146309337531E-5
26-27	2.8238698234694525E-4	5.260908164271857E-4	0.0	0.003206832991309831	4.64197779200458E-5
28-29	3.017285564802977E-4	5.338274460805267E-4	0.0	0.01608832136412254	4.64197779200458E-5
30-31	3.210701306136501E-4	5.957204833072545E-4	0.0	0.02519433446610486	5.028809274671628E-5
32-33	3.3654338992033205E-4	5.957204833072545E-4	0.0	0.03409145856744697	6.96296668800687E-5
34-35	3.59753278880355E-4	6.692184650139936E-4	0.0	0.04659385208724597	6.96296668800687E-5
36-37	3.7909485301370737E-4	7.427164467207328E-4	0.0	0.06360283238011609	7.349798170673918E-5
38-39	3.7909485301370737E-4	7.736629653340968E-4	0.0	0.09453387773417327	7.736629653340966E-5
40-41	3.9843642714705975E-4	8.278193729074835E-4	0.0	0.15125884635246922	7.736629653340966E-5
42-43	4.4098789024043513E-4	8.703708360008588E-4	0.0	0.17908750321553668	7.736629653340966E-5
44-45	4.4098789024043513E-4	0.0010173667994143373	0.0	0.22062933613915103	7.736629653340966E-5
46-47	4.4098789024043513E-4	0.0011218112997344403	0.0	0.2662522412049027	8.123461136008014E-5
48-49	4.487245198937761E-4	0.0011256796145611109	0.0	0.30599530773411526	8.510292618675064E-5
50-51	4.758027236804695E-4	0.0012262558000545433	0.0	0.3451619953541539	8.510292618675064E-5
52-53	4.835393533338104E-4	0.0012301241148812137	0.0	0.3932606219089747	8.897124101342112E-5
54-55	5.028809274671629E-4	0.001233992429707884	0.0	0.48850240125642863	9.28395558400916E-5
56-57	5.260908164271857E-4	0.0012533340038412366	0.0	0.5860613011850582	9.28395558400916E-5
58-59	5.415640757338677E-4	0.0012610706334945775	0.0	0.6647041416112691	9.28395558400916E-5
60-61	5.454323905605381E-4	0.0013307003003746464	0.0	0.7113830966247019	9.28395558400916E-5
62-63	5.647739646938906E-4	0.0013809883931213626	0.0	0.7399738115086234	1.0057618549343257E-4
64-65	5.647739646938906E-4	0.0015318526713615115	0.0	0.761439090481818	1.0057618549343257E-4
66-67	5.725105943472316E-4	0.001543457615841523	0.0	0.7736977801675367	1.0444450032010305E-4
68-69	5.879838536539134E-4	0.001586009078934898	0.0	0.7860570460387489	1.0831281514677353E-4
70-71	6.073254277872658E-4	0.001624692227201603	0.0	0.7965285742745459	1.1218112997344402E-4
72-73	6.537452057073117E-4	0.0016362971716816143	0.0	0.8041955742610067	1.3152270410679643E-4
74-75	6.576135205339822E-4	0.0017175317830416946	0.0	0.8118045495250676	1.3152270410679643E-4
76-77	6.614818353606526E-4	0.0018297129130151386	0.0	0.8187249647499811	1.3152270410679643E-4
78-79	6.653501501873232E-4	0.0018761326909351845	0.0	0.8253049682701477	1.3152270410679643E-4
80-81	6.653501501873232E-4	0.001880001005761855	0.0	0.8297883451542587	1.3152270410679643E-4
82-83	6.730867798406641E-4	0.001887737635415196	0.0	0.8331769889424221	1.4312764858680788E-4
84-85	6.96296668800687E-4	0.0019032108947218778	0.0	0.8364070318226919	1.4699596341347836E-4
86-87	6.96296668800687E-4	0.0019264207836819007	0.0	0.8369176493798125	1.4699596341347836E-4
88-89	6.96296668800687E-4	0.0019883138209086286	0.0	0.8370104889356526	1.4699596341347836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15265	0.0	44.751556	1
GTATCAA	25975	0.0	32.753807	1
TCAACGC	31980	0.0	25.830803	4
ATCAACG	32075	0.0	25.793396	3
CAACGCA	32535	0.0	25.313072	5
AACGCAG	33315	0.0	24.858452	6
TATCAAC	33935	0.0	24.627361	2
ACGCAGA	38505	0.0	21.442696	7
CGCAGAG	39725	0.0	20.805214	8
GTACATG	37990	0.0	20.0178	1
TACATGG	38145	0.0	19.487093	2
ACATGGG	38325	0.0	18.892748	3
AGAGTAC	41915	0.0	18.022629	10-11
GCAGAGT	46215	0.0	17.838297	9
CATGGGG	26880	0.0	17.387691	4
GAGTACT	24320	0.0	16.914568	12-13
CAGAGTA	45825	0.0	16.233976	10-11
GTGGTAT	7820	0.0	15.878248	1
AGTACTT	27975	0.0	15.235144	12-13
TATACCG	1105	0.0	14.7547455	5
>>END_MODULE