FastQCFastQC Report
Fri 27 May 2016
SRR2140186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17565803
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215900.047.7241941
GTATCAA384350.034.430511
TCAACGC472400.027.329814
ATCAACG476900.027.0458973
CAACGCA488500.026.4458665
AACGCAG495750.026.0758066
TATCAAC504150.025.8466822
ACGCAGA577600.022.3520247
GTACATG563650.021.936281
CGCAGAG602900.021.4346378
TACATGG572550.021.22672
ACATGGG582900.020.3386653
GTGGTAT105450.018.6789421
GCAGAGT685150.018.6440569
GAGTACT361850.018.13225612-13
AGAGTAC623350.017.99416210-11
CATGGGG416300.017.4645464
TGGTATC104900.017.317332
CAGAGTA677450.016.85038810-11
GTACTTT418400.015.67782214-15
Produced by FastQC (version 0.11.5)