##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17565803
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14497435727817	33.0	31.0	34.0	30.0	34.0
2	32.27111843392528	34.0	31.0	34.0	30.0	34.0
3	32.32133202222523	34.0	31.0	34.0	30.0	34.0
4	35.960966828558874	37.0	35.0	37.0	35.0	37.0
5	35.82931853442737	37.0	35.0	37.0	35.0	37.0
6	35.85483117395772	37.0	35.0	37.0	35.0	37.0
7	35.77003522127625	37.0	35.0	37.0	33.0	37.0
8	35.73514749083774	37.0	35.0	37.0	33.0	37.0
9	37.36268082933641	39.0	37.0	39.0	34.0	39.0
10-11	37.25778696254307	39.0	37.0	39.0	33.5	39.0
12-13	37.216948977510455	39.0	37.0	39.0	33.0	39.0
14-15	38.39688575580632	40.0	38.0	41.0	33.0	41.0
16-17	38.42422472801272	40.0	38.0	41.0	33.0	41.0
18-19	38.43438239060292	40.0	38.0	41.0	33.5	41.0
20-21	38.40732443303068	40.0	38.0	41.0	34.0	41.0
22-23	38.30487443537608	40.0	38.0	41.0	33.5	41.0
24-25	38.214328942712314	40.0	38.0	41.0	33.0	41.0
26-27	38.04433235648751	40.0	37.0	41.0	33.0	41.0
28-29	37.911393223897704	40.0	37.0	41.0	33.0	41.0
30-31	37.752352215813204	40.0	37.0	41.0	32.5	41.0
32-33	37.923717657090485	40.0	37.0	41.0	33.0	41.0
34-35	37.899346461049056	40.0	37.0	41.0	33.0	41.0
36-37	37.81188734330806	40.0	37.0	41.0	33.0	41.0
38-39	37.70235743157679	40.0	37.0	41.0	33.0	41.0
40-41	37.64504812160236	40.0	37.0	41.0	33.0	41.0
42-43	37.53112579439593	40.0	36.5	41.0	32.0	41.0
44-45	37.56773144608789	39.5	36.5	41.0	32.5	41.0
46-47	37.702938826146195	40.0	37.0	41.0	32.5	41.0
48-49	37.620509570543625	40.0	36.5	41.0	32.0	41.0
50-51	37.493578940163594	40.0	36.0	41.0	32.0	41.0
52-53	37.33041972817739	39.0	36.0	41.0	31.0	41.0
54-55	37.21363906325354	39.0	36.0	41.0	31.0	41.0
56-57	37.04250566684523	39.0	35.0	40.5	31.0	41.0
58-59	36.8849981888768	39.0	35.0	40.0	31.0	41.0
60-61	36.649354449523926	38.0	35.0	40.0	31.0	41.0
62-63	36.379429699813755	38.0	35.0	40.0	30.0	41.0
64-65	36.151818015600846	37.5	34.5	40.0	30.0	41.0
66-67	35.85923483753025	37.0	34.0	40.0	29.5	41.0
68-69	35.545367360472085	36.5	34.0	39.0	29.0	41.0
70-71	35.25178779106972	36.0	34.0	39.0	29.0	40.5
72-73	34.954697446602054	36.0	34.0	39.0	29.0	40.0
74-75	34.57294779844035	35.0	33.0	38.0	29.0	40.0
76-77	33.512962697321925	34.5	31.5	37.0	27.5	39.0
78-79	33.86425385725834	35.0	33.0	37.0	28.0	39.0
80-81	33.74290123966838	35.0	33.0	36.0	29.0	39.0
82-83	33.51432445072301	35.0	33.0	36.0	29.0	37.5
84-85	33.28836557032672	35.0	32.5	35.5	28.0	37.0
86-87	33.07144240584056	34.5	32.0	35.0	28.0	37.0
88-89	32.834796876988825	34.0	32.0	35.0	27.0	36.0
90-91	32.687720428403786	34.0	32.0	35.0	27.0	36.0
92-93	32.589467593516005	34.0	32.0	35.0	27.0	36.0
94-95	32.532158291745404	34.0	32.0	35.0	27.0	35.0
96-97	32.471459434109036	34.0	32.0	35.0	27.0	35.0
98-99	32.60232147212976	34.0	32.0	35.0	28.5	35.0
100-101	31.667267077037963	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	9.0
19	23.0
20	102.0
21	741.0
22	3353.0
23	11693.0
24	30795.0
25	64581.0
26	115064.0
27	179062.0
28	254394.0
29	335267.0
30	423636.0
31	525112.0
32	651183.0
33	826916.0
34	1100711.0
35	1529343.0
36	2314539.0
37	3523783.0
38	3718714.0
39	1879615.0
40	77164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.913575428347905	22.02119652599998	15.304401398558323	23.76082664709379
2	14.061126396695354	22.336815713922178	41.26938664671855	22.332671242663913
3	16.90143058077106	27.60896840298163	31.577383624306844	23.912217391940466
4	11.956322920731754	18.92351216729616	39.43390925571859	29.686255656253497
5	12.412304749176567	38.611414462521296	36.37190397729042	12.604376811011713
6	29.267788099411113	39.40207572634169	18.688732874893336	12.641403299353865
7	25.580259553178415	33.13927635417521	24.13855489555473	17.141909197091646
8	24.08870804255291	37.636662553940745	21.19530772376304	17.079321679743305
9	25.41678851800854	16.278965442115002	21.538485886469296	36.765760153407165
10-11	23.85999660818239	27.095578835764012	29.562286449415375	19.48213810663822
12-13	24.537452116478818	24.803728016305318	29.631551714430586	21.027268152785272
14-15	21.750454562196786	26.127311116946945	26.97504919074864	25.14718513010763
16-17	21.363743121465976	29.38178739167493	27.57372250490009	21.680746981959004
18-19	21.43551308186708	28.448542887563978	29.31315465623746	20.80278937433148
20-21	22.356873373063706	27.558557608400626	29.077213192009687	21.00735582652598
22-23	22.078025120937763	27.521051549457017	28.881776128954122	21.519147200651098
24-25	21.742037595437242	27.720605867434656	28.986911736409866	21.550444800718235
26-27	21.800809867460693	27.750102273258193	28.87417939242278	21.574908466858336
28-29	21.79501525419091	27.73297983437004	28.9128909313949	21.559113980044145
30-31	21.971117789881962	27.673324753245907	28.72361158647989	21.63194587039224
32-33	21.796564633748975	27.841262781678182	28.671903764605734	21.69026881996711
34-35	21.921679211019015	27.84775881217832	28.7117991942016	21.518762782601065
36-37	21.959980756254268	27.779810999214494	28.566423785625343	21.693784458905892
38-39	21.737615098028982	27.975887192844734	28.653016274647193	21.633481434479094
40-41	21.924478428470497	27.762764964188687	28.580818429310728	21.731938178030088
42-43	21.79297500192788	27.941102027086806	28.30641716062133	21.95950581036399
44-45	21.89803729660765	27.928242592415824	28.16987181354415	22.00384829743237
46-47	22.06955186859703	27.907425089103	27.988397995013624	22.034625047286344
48-49	21.983111798667114	28.116839585757496	27.80414574617385	22.095902869401538
50-51	22.00772470516415	28.113881500814347	27.773929432293958	22.10446436172755
52-53	22.13500601120562	28.052961055411902	27.652541855585255	22.159491077797224
54-55	22.155883937717853	27.886205879976377	27.742828423768877	22.21508175853689
56-57	22.19014553966241	27.960099198521597	27.66603674222542	22.183718519590574
58-59	22.04953847794798	27.995012993574992	27.679835218686645	22.275613309790383
60-61	22.153504750287027	27.959181071768462	27.738201245209066	22.149112932735452
62-63	22.21637512806692	28.02307294436529	27.605334867887656	22.155217059680137
64-65	22.124805029793272	27.999745941717656	27.63417503100382	22.24127399748525
66-67	22.21963193572448	28.013708485687307	27.494108731934652	22.272550846653562
68-69	22.153406557020265	27.95063582138727	27.534784951674396	22.361172669918066
70-71	22.175118239575333	27.791706121556025	27.5465451867838	22.48663045208484
72-73	22.187178772455756	27.827625225222686	27.43969776023021	22.545498242091348
74-75	22.10561196022853	27.83798892543374	27.46957760375911	22.586821510578613
76-77	22.28316775851171	27.7626516395907	27.41551302049816	22.53866758139943
78-79	22.09728265985685	27.782916644886	27.406719690946467	22.713081004310677
80-81	22.28666469566202	27.764026558396733	27.33642271926645	22.612886026674804
82-83	22.228181131332775	27.711997373773873	27.521189431187615	22.53863206370574
84-85	22.301601123863378	27.696107491461163	27.47530449066225	22.52698689401321
86-87	22.203813401208222	27.712665847200007	27.515308474084467	22.568212277507303
88-89	22.217095078526107	27.600439944932475	27.539102238072232	22.643362738469186
90-91	22.2083956416302	27.60731043242213	27.55567787859235	22.628616047355322
92-93	22.253275272282075	27.508030807886602	27.63650956009649	22.602184359734835
94-95	22.09563220120066	27.54075070713771	27.776523113508745	22.587093978152886
96-97	21.964938036704954	27.55901408158335	27.82464866707918	22.651399214632516
98-99	22.133642715584916	28.21491313951659	27.89494607171241	21.756498073186084
100-101	25.154669404397943	35.88375188874605	29.049783486262058	9.911795220593946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3293.0
1	3005.0
2	4263.5
3	6085.0
4	7954.0
5	12503.0
6	16498.5
7	19867.5
8	22455.5
9	22670.0
10	21161.0
11	19528.0
12	21537.5
13	28582.0
14	45519.0
15	69556.0
16	89327.0
17	99761.5
18	102111.5
19	99329.5
20	93386.0
21	87372.5
22	85686.0
23	93090.5
24	111091.5
25	140153.0
26	178929.5
27	224221.0
28	282009.0
29	349275.0
30	412095.5
31	472809.0
32	526658.0
33	570241.5
34	605535.5
35	629049.5
36	651142.5
37	666542.0
38	668618.5
39	663900.5
40	656165.5
41	650111.0
42	640957.5
43	631711.0
44	627843.5
45	631892.5
46	637088.0
47	640638.0
48	646680.5
49	648999.0
50	643175.5
51	632812.5
52	611107.5
53	577416.0
54	547726.0
55	519435.5
56	486653.0
57	448773.0
58	404976.0
59	356105.5
60	306471.0
61	259700.5
62	213442.0
63	172182.5
64	138784.5
65	116016.5
66	99878.0
67	73754.0
68	50269.0
69	39863.0
70	28895.0
71	17763.0
72	11881.0
73	8485.5
74	6093.0
75	4305.0
76	2865.5
77	1803.0
78	1240.5
79	876.5
80	609.5
81	424.5
82	326.5
83	231.5
84	146.5
85	107.0
86	82.5
87	60.0
88	38.5
89	23.5
90	16.5
91	12.5
92	11.5
93	7.5
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013264409261563505
3	0.0
4	3.8711580677524394E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0031851660866286614
18-19	0.0
20-21	0.002040989909140936
22-23	0.0
24-25	3.1038836247528854E-4
26-27	0.003407093294760631
28-29	0.0
30-31	1.2842432034710412E-4
32-33	8.125912734564199E-4
34-35	7.866595855819883E-4
36-37	0.010248925652255656
38-39	0.011563899836680632
40-41	0.023955150377848313
42-43	0.0019840190957846277
44-45	0.0013115289308344455
46-47	0.0017503917219471006
48-49	0.0016207987253304876
50-51	8.346547412021593E-5
52-53	1.1864721073052867E-4
54-55	0.005910920442856878
56-57	0.0035049451111257186
58-59	0.0037733032895471185
60-61	0.0015812901488753703
62-63	3.991637486477091E-4
64-65	6.908788900653485E-4
66-67	6.523558496377228E-4
68-69	1.370511284310236E-5
70-71	0.0
72-73	1.268610426864151E-4
74-75	2.215508675074355E-4
76-77	0.0016092619841178166
78-79	0.0018356117358199362
80-81	0.011093613917043317
82-83	0.009665036945401545
84-85	0.012619361830109341
86-87	0.006272396931722408
88-89	0.08650915264756617
90-91	3.588129537837091E-4
92-93	2.5012892061450005E-5
94-95	2.6926295420867186E-4
96-97	0.015031993327985289
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	489.0
20-21	1626.0
22-23	3340.0
24-25	6737.0
26-27	8368.0
28-29	15450.0
30-31	42219.0
32-33	54827.0
34-35	78489.0
36-37	124192.0
38-39	136622.0
40-41	143788.0
42-43	166337.0
44-45	194403.0
46-47	193510.0
48-49	178856.0
50-51	164670.0
52-53	150121.0
54-55	165809.0
56-57	174370.0
58-59	179393.0
60-61	183152.0
62-63	173098.0
64-65	184317.0
66-67	197996.0
68-69	203346.0
70-71	202940.0
72-73	196412.0
74-75	196739.0
76-77	198792.0
78-79	213913.0
80-81	217728.0
82-83	209163.0
84-85	211020.0
86-87	224980.0
88-89	241343.0
90-91	265457.0
92-93	282612.0
94-95	356675.0
96-97	460289.0
98-99	1490466.0
100-101	9471749.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.13012001079369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.75347413530437	73.78086956242727
2	7.692792304070233	12.790054949109372
3	1.9306973133866847	4.814972980990561
4	0.7169704089511585	2.3840734456119055
5	0.334579484387226	1.3906816345129787
6	0.18923378242586125	0.9438616225894961
7	0.10538086722938465	0.6132226897734183
8	0.06491943662359337	0.43174084460419454
9	0.04775323459004876	0.35727589101369045
>10	0.15879967214911708	2.0521493291801916
>50	0.0039132505289748	0.22287154677597504
>100	0.0014593857416040808	0.20567378089325572
>500	2.6724611836431058E-5	0.012551722517640352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.985015657980452E-5	0.0	0.0	0.0
3	0.0	3.985015657980452E-5	0.0	0.0	0.0
4	0.0	8.53931926710097E-5	0.0	5.692879511400646E-6	0.0
5	0.0	8.53931926710097E-5	0.0	5.692879511400646E-6	0.0
6	2.2771518045602583E-5	8.53931926710097E-5	0.0	1.1385759022801292E-5	0.0
7	2.2771518045602583E-5	8.53931926710097E-5	0.0	1.1385759022801292E-5	0.0
8	2.2771518045602583E-5	9.677895169381098E-5	0.0	1.1385759022801292E-5	0.0
9	2.2771518045602583E-5	2.220223009446252E-4	0.0	3.4157277068403876E-5	5.692879511400646E-6
10-11	2.2771518045602583E-5	2.220223009446252E-4	0.0	6.26216746254071E-5	1.1385759022801292E-5
12-13	2.2771518045602583E-5	2.220223009446252E-4	0.0	7.970031315960904E-5	1.1385759022801292E-5
14-15	2.2771518045602583E-5	3.358798911726381E-4	0.0	8.823963242671001E-5	1.1385759022801292E-5
16-17	3.7003716824104196E-5	3.501120899511397E-4	0.0	1.0816471071661227E-4	1.7078638534201938E-5
18-19	3.985015657980452E-5	4.212730838436478E-4	5.692879511400646E-6	1.2808978900651454E-4	2.2771518045602583E-5
20-21	3.985015657980452E-5	4.8674119822475525E-4	5.692879511400646E-6	1.7647926485342001E-4	2.5617957801302906E-5
22-23	4.8389475846905485E-5	5.721343908957649E-4	5.692879511400646E-6	4.098873248208465E-4	3.985015657980452E-5
24-25	5.977523486970678E-5	6.575275835667746E-4	5.692879511400646E-6	0.0013065158478664483	6.26216746254071E-5
26-27	6.831455413680775E-5	6.888384208794782E-4	5.692879511400646E-6	0.00370037168241042	6.26216746254071E-5
28-29	6.831455413680775E-5	6.888384208794782E-4	5.692879511400646E-6	0.01946110860972311	6.26216746254071E-5
30-31	6.831455413680775E-5	7.941566918403901E-4	5.692879511400646E-6	0.02890274927938108	6.26216746254071E-5
32-33	6.831455413680775E-5	8.055424508631914E-4	5.692879511400646E-6	0.03480057245319215	6.26216746254071E-5
34-35	6.831455413680775E-5	8.738570049999991E-4	5.692879511400646E-6	0.04282468612451136	6.831455413680775E-5
36-37	6.831455413680775E-5	9.421715591368068E-4	5.692879511400646E-6	0.05333658814231265	7.116099389250808E-5
38-39	7.400743364820839E-5	9.734823964495105E-4	5.692879511400646E-6	0.07232803419234521	7.685387340390872E-5
40-41	7.400743364820839E-5	0.0010816471071661228	5.692879511400646E-6	0.10410853406473931	7.970031315960904E-5
42-43	8.53931926710097E-5	0.001101572185456025	5.692879511400646E-6	0.11975256696206829	7.970031315960904E-5
44-45	9.393251193811066E-5	0.0012382012937296404	5.692879511400646E-6	0.14249846704986957	7.970031315960904E-5
46-47	9.677895169381098E-5	0.0013662910827361551	5.692879511400646E-6	0.169260693632964	7.970031315960904E-5
48-49	9.677895169381098E-5	0.0013719839622475557	5.692879511400646E-6	0.19149423456473924	9.108607218241033E-5
50-51	9.677895169381098E-5	0.0015114595102768715	5.692879511400646E-6	0.21477241888685647	9.108607218241033E-5
52-53	1.0247183120521162E-4	0.0015313845885667737	5.692879511400646E-6	0.24076326029615613	9.677895169381097E-5
54-55	1.0531827096091195E-4	0.0015456167873452754	5.692879511400646E-6	0.30909773951125374	1.1101115047231259E-4
56-57	1.1385759022801291E-4	0.0015826205041693795	5.692879511400646E-6	0.36266488927377816	1.223969094951139E-4
58-59	1.1385759022801291E-4	0.0016110849017263828	5.692879511400646E-6	0.418896306647638	1.3093622876221485E-4
60-61	1.252433492508142E-4	0.0016281635402605847	5.692879511400646E-6	0.4522508877049344	1.366291082736155E-4
62-63	1.4516842754071647E-4	0.0016509350583061872	5.692879511400646E-6	0.46898795346845235	1.366291082736155E-4
64-65	1.4801486729641678E-4	0.0018388000821824087	5.692879511400646E-6	0.4854659932142015	1.366291082736155E-4
66-67	1.4801486729641678E-4	0.0018558787207166106	5.692879511400646E-6	0.49483362645021123	1.366291082736155E-4
68-69	1.4801486729641678E-4	0.0018928824375407148	5.692879511400646E-6	0.5029061295973773	1.366291082736155E-4
70-71	1.5370774680781743E-4	0.0019185003953420177	5.692879511400646E-6	0.510879007353094	1.366291082736155E-4
72-73	1.5940062631921809E-4	0.0019355790338762195	5.692879511400646E-6	0.5174571296285173	1.5940062631921809E-4
74-75	1.5940062631921809E-4	0.001975429190456024	5.692879511400646E-6	0.5233748778806184	1.5940062631921809E-4
76-77	1.5940062631921809E-4	0.0020437437445928316	5.692879511400646E-6	0.5287176453020679	1.622470660749184E-4
78-79	1.5940062631921809E-4	0.002077901021661236	5.692879511400646E-6	0.5336818362360093	1.6509350583061874E-4
80-81	1.5940062631921809E-4	0.002092133220439737	5.692879511400646E-6	0.5365937441060906	1.7932570460912035E-4
82-83	1.6509350583061874E-4	0.0021006725397068383	5.692879511400646E-6	0.5382816828812209	1.9640434314332228E-4
84-85	1.707863853420194E-4	0.0021035189794625384	5.692879511400646E-6	0.5398272996685662	1.992507828990226E-4
86-87	1.7647926485342001E-4	0.002134829816775242	5.692879511400646E-6	0.5400464755297552	2.0209722265472293E-4
88-89	1.7647926485342001E-4	0.0021974514914006493	5.692879511400646E-6	0.5400948650056021	2.0494366241042325E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21590	0.0	47.724194	1
GTATCAA	38435	0.0	34.43051	1
TCAACGC	47240	0.0	27.32981	4
ATCAACG	47690	0.0	27.045897	3
CAACGCA	48850	0.0	26.445866	5
AACGCAG	49575	0.0	26.075806	6
TATCAAC	50415	0.0	25.846682	2
ACGCAGA	57760	0.0	22.352024	7
GTACATG	56365	0.0	21.93628	1
CGCAGAG	60290	0.0	21.434637	8
TACATGG	57255	0.0	21.2267	2
ACATGGG	58290	0.0	20.338665	3
GTGGTAT	10545	0.0	18.678942	1
GCAGAGT	68515	0.0	18.644056	9
GAGTACT	36185	0.0	18.132256	12-13
AGAGTAC	62335	0.0	17.994162	10-11
CATGGGG	41630	0.0	17.464546	4
TGGTATC	10490	0.0	17.31733	2
CAGAGTA	67745	0.0	16.850388	10-11
GTACTTT	41840	0.0	15.677822	14-15
>>END_MODULE