FastQCFastQC Report
Fri 27 May 2016
SRR2140185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23300110
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303250.050.0861241
GTATCAA538150.033.4481121
TCAACGC646000.027.1657264
ATCAACG654900.026.796553
CAACGCA664450.026.3927925
AACGCAG675500.025.9915736
TATCAAC685650.025.8191342
ACGCAGA780200.022.4613177
CGCAGAG815100.021.479368
GTACATG680900.020.9000071
TACATGG698750.020.0589122
ACATGGG693200.019.6056353
GCAGAGT918600.018.8529689
GAGTACT545750.018.48842612-13
CAGAGTA905750.017.74342710-11
GTGGTAT143250.017.6954731
AGAGTAC841250.017.57700510-11
CATGGGG488500.017.286924
TGGTATC140800.016.1561682
GTACTTT627100.016.05503814-15
Produced by FastQC (version 0.11.5)