##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23300110
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41045625964856	31.0	31.0	34.0	30.0	34.0
2	31.57037941022596	31.0	31.0	34.0	30.0	34.0
3	31.61635391420899	31.0	31.0	34.0	30.0	34.0
4	35.42019187892246	37.0	35.0	37.0	33.0	37.0
5	35.23144212623889	37.0	35.0	37.0	32.0	37.0
6	35.22317255154589	37.0	35.0	37.0	32.0	37.0
7	35.12665129048747	37.0	35.0	37.0	32.0	37.0
8	35.06024181860086	37.0	35.0	37.0	32.0	37.0
9	36.59806009499526	39.0	35.0	39.0	32.0	39.0
10-11	36.532858986502646	38.5	35.0	39.0	32.0	39.0
12-13	36.48214173666992	38.0	35.0	39.0	32.0	39.0
14-15	37.56100462615842	39.0	36.0	41.0	32.0	41.0
16-17	37.53185615861899	39.0	36.0	41.0	32.0	41.0
18-19	37.54603722900879	39.0	36.0	41.0	32.0	41.0
20-21	37.51876572145575	39.0	36.0	41.0	32.0	41.0
22-23	37.437960231283874	39.0	36.0	41.0	32.0	41.0
24-25	37.31695882812416	39.0	36.0	41.0	31.0	41.0
26-27	37.07859238998902	39.0	36.0	40.5	31.0	41.0
28-29	36.92024125627835	39.0	36.0	40.0	30.5	41.0
30-31	36.74503380532039	38.5	35.5	40.0	30.0	41.0
32-33	36.516277592036005	38.0	35.0	40.0	30.0	41.0
34-35	36.358241915568826	38.0	35.0	40.0	30.0	41.0
36-37	36.19673161528257	38.0	35.0	40.0	30.0	41.0
38-39	36.027639882744865	38.0	34.5	40.0	29.5	41.0
40-41	35.864337340674396	38.0	34.0	40.0	29.0	41.0
42-43	35.64946668948713	38.0	34.0	40.0	28.0	41.0
44-45	36.08090222725062	38.0	34.5	40.0	29.5	41.0
46-47	36.33318538354253	38.0	35.0	40.0	30.0	41.0
48-49	36.38171381753584	38.0	35.0	40.0	30.0	41.0
50-51	36.306919125757396	38.0	35.0	40.0	29.5	41.0
52-53	36.16892033127584	38.0	34.0	40.0	29.0	41.0
54-55	36.03281887375017	38.0	34.0	40.0	28.5	41.0
56-57	35.909940954608665	38.0	34.0	40.0	28.0	41.0
58-59	35.7241664907601	38.0	34.0	40.0	28.0	41.0
60-61	35.5372052587064	37.5	34.0	40.0	28.0	41.0
62-63	35.308632325818294	37.0	33.0	40.0	28.0	41.0
64-65	35.0416772114726	37.0	33.0	39.5	28.0	41.0
66-67	34.7361234819393	36.0	33.0	39.0	27.0	41.0
68-69	34.47227413110235	36.0	32.0	39.0	26.5	40.0
70-71	34.191540760897745	35.0	32.0	39.0	26.0	40.0
72-73	33.89170548345044	35.0	32.0	38.0	26.0	40.0
74-75	33.56505927809516	35.0	31.0	37.5	26.0	39.5
76-77	31.87080429078761	33.5	29.5	35.5	25.0	38.5
78-79	32.89669584879199	34.0	31.0	36.0	26.0	39.0
80-81	32.97829498993897	34.0	31.0	36.0	26.0	39.0
82-83	32.7569146034066	34.0	31.0	36.0	26.0	37.5
84-85	32.51876646248722	34.0	31.0	35.0	26.0	37.0
86-87	32.31270646008794	34.0	31.0	35.0	26.0	36.5
88-89	32.109384778983184	34.0	31.0	35.0	26.0	36.0
90-91	31.961171652161866	34.0	31.0	35.0	26.0	36.0
92-93	31.92261716631824	34.0	31.0	35.0	26.0	35.5
94-95	31.9130354742434	34.0	31.0	35.0	26.0	35.0
96-97	32.02440533148312	34.0	31.0	35.0	26.0	35.0
98-99	32.2336873232889	34.0	31.0	35.0	27.0	35.0
100	30.327845494403174	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	8.0
19	64.0
20	371.0
21	2339.0
22	10422.0
23	34382.0
24	84928.0
25	170567.0
26	291830.0
27	437810.0
28	598707.0
29	761174.0
30	923945.0
31	1096447.0
32	1298487.0
33	1551170.0
34	1888506.0
35	2355516.0
36	3032164.0
37	3785547.0
38	3458513.0
39	1472525.0
40	44685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12705519416003	21.730438182480682	14.879037051756407	23.263469571602883
2	14.65240289234288	22.34602309728735	41.160349685361204	21.84122432500856
3	17.54791361705647	27.62765187064146	31.597006692114615	23.22742782018745
4	12.391814189439303	19.016110463163745	39.546563691318596	29.04551165607836
5	12.340139166724965	38.77620749429938	36.52555288365591	12.358100455319738
6	28.84110847545355	39.66135782191586	18.887807825799964	12.609725876830623
7	25.191533430528867	33.48230115651815	24.40688906618896	16.91927634676403
8	23.84010820905659	37.74618313591415	21.434803472588488	16.97890518244077
9	25.26938284840715	16.555634286705086	21.80222754313177	36.37275532175599
10-11	23.631770290506807	27.262247024777743	29.806891882217883	19.299090802497567
12-13	24.36324043838129	24.991832646769225	29.814822110926475	20.830104803923007
14-15	21.592381557325822	26.367558421311553	27.220569923975447	24.81949009738718
16-17	21.077601803711175	29.51742923518198	27.876321338048594	21.52864762305825
18-19	21.14846783516503	28.619292858091967	29.596033133586015	20.636206173156996
20-21	21.986308328379163	27.74269189279347	29.48672369770075	20.784276081126617
22-23	21.769800319641227	27.613275454570296	29.321683314441575	21.295240911346898
24-25	21.558461636906078	27.832200356706196	29.32075728561447	21.28858072077326
26-27	21.490522496777224	27.91369461999872	29.25349847496245	21.342284408261605
28-29	21.54413058189394	27.8871944912266	29.28213618692212	21.286538739957344
30-31	21.75887163399821	27.802676367005123	29.114527315141842	21.323924683854823
32-33	21.625408738979342	27.949480259574738	29.026126464237628	21.398984537208293
34-35	21.723632700840614	27.991038407028295	28.99617868606582	21.289150206065266
36-37	21.780484764041766	27.900186327389363	28.885402937036915	21.433925971531956
38-39	21.593080446689694	28.087601224867477	28.952764715005042	21.36655361343779
40-41	21.770867274684992	27.93534349456411	28.85470192613347	21.439087304617438
42-43	21.67343066029477	28.09666605304454	28.568866887132383	21.661036399528307
44-45	21.732302207821576	28.17249781977405	28.39910352027121	21.696096452133155
46-47	21.897578730108403	28.138457290336284	28.17782706963917	21.78613690991614
48-49	21.836261563675414	28.271215309894142	28.109743439317942	21.782779687112498
50-51	21.87098166516345	28.328811671225974	28.006941707379205	21.79326495623137
52-53	22.040984864840905	28.230620988451054	27.899108477572504	21.82928566913554
54-55	22.02510686112235	28.151170271635834	27.95236284623675	21.871360021005067
56-57	21.94568563552929	28.285386165862096	27.90103702336715	21.86789117524146
58-59	21.900346147108007	28.29760168726059	27.940054169848533	21.861997995782865
60-61	21.99496470024473	28.300663164814015	27.90746572304305	21.79690641189821
62-63	21.97796060522273	28.328717831439796	27.904167046707144	21.789154516630333
64-65	21.97645835137382	28.22140972589636	27.89060681612617	21.911525106603644
66-67	22.041627802303726	28.23970183585897	27.773767793311983	21.944902568525322
68-69	21.925124327996322	28.22450102976048	27.87836741122795	21.972007231015247
70-71	21.96806444826297	28.048359096253577	27.906085897102457	22.077490558380997
72-73	21.99311630502434	28.12900670366702	27.796759623422883	22.081117367885756
74-75	21.86047164702028	28.19135202358904	27.847294414987868	22.10088191440282
76-77	21.98306980809021	28.134756356266617	27.85434662876413	22.027827206879042
78-79	21.867880070774085	28.14620528867645	27.831562143492267	22.154352497057197
80-81	21.945116309848547	28.138622201951087	27.818868716871805	22.09739277132857
82-83	22.00412324581352	28.044014018706577	27.973351210361674	21.97851152511823
84-85	22.00994846357015	28.050724722569058	27.92716239979821	22.012164414062582
86-87	21.8531362956965	28.10399542362379	28.006609968727158	22.036258311952555
88-89	21.976977661539358	27.963292015527998	27.96487399794672	22.09485632498593
90-91	21.96915483172151	28.01405126883068	28.044466358786174	21.972327540661638
92-93	21.987435664766426	27.933267327990958	28.089690803956774	21.989606203285845
94-95	21.754493449940725	27.966490798044703	28.233892584457827	22.045123167556746
96-97	21.76931550678283	28.07249337265864	28.362003236120376	21.796187884438154
98-99	22.784820804165385	30.036647744380584	28.642010501218163	18.53652095023587
100	22.376028218561302	30.418326422628855	22.458841413842457	24.746803944967382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5716.0
1	4948.5
2	6476.5
3	9134.0
4	11755.5
5	19010.5
6	25995.0
7	31850.5
8	36203.0
9	36297.0
10	32903.0
11	28761.5
12	30101.0
13	38665.0
14	58847.0
15	91215.5
16	119779.5
17	135839.0
18	143276.0
19	142907.5
20	137462.0
21	131432.0
22	127453.5
23	133848.5
24	155163.5
25	192026.5
26	244066.0
27	302605.5
28	370373.0
29	451987.5
30	533562.0
31	609425.5
32	679381.5
33	738560.5
34	789725.0
35	832514.0
36	872287.0
37	900226.5
38	905188.0
39	901752.5
40	899080.5
41	890691.0
42	877752.5
43	867884.0
44	863440.0
45	867033.0
46	870286.5
47	874377.0
48	875658.5
49	866251.0
50	851400.5
51	828382.0
52	793740.5
53	748645.5
54	703948.5
55	662962.5
56	620264.5
57	570514.5
58	513537.5
59	450905.0
60	383473.5
61	320369.0
62	262495.5
63	211431.5
64	167234.5
65	130477.0
66	102984.0
67	78879.0
68	58622.5
69	44536.5
70	33660.0
71	24294.5
72	17262.0
73	12221.5
74	8772.5
75	6200.0
76	4278.5
77	2874.0
78	2041.0
79	1471.5
80	1011.0
81	688.5
82	517.5
83	361.5
84	246.5
85	190.0
86	156.0
87	124.0
88	90.0
89	64.0
90	41.0
91	28.5
92	21.5
93	13.0
94	8.0
95	5.5
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11619687632375986
3	4.291825231726374E-6
4	1.502138831104231E-4
5	0.0
6	0.0
7	0.0
8	1.1587928125661211E-4
9	0.0
10-11	8.583650463452748E-6
12-13	1.2017110648833846E-4
14-15	3.111573293001621E-4
16-17	6.394819595272297E-4
18-19	2.575095139035824E-5
20-21	7.511193663660036E-5
22-23	4.2928486014682705E-6
24-25	0.006140844344625902
26-27	2.1484087692288225E-5
28-29	0.0022405332899274803
30-31	0.00205022310066912
32-33	0.022922055230125235
34-35	0.015571550679383397
36-37	0.00827317438019645
38-39	0.004129744600627598
40-41	8.354157765970912E-4
42-43	2.4066467840502692E-4
44-45	0.001026719843549511
46-47	0.0017169474186902432
48-49	0.001173490382855149
50-51	6.274219066543067E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.0808250388864575E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	9.24103673342905E-5
70-71	4.944083071004189E-4
72-73	7.914475752908696E-6
74-75	5.0815360542070456E-5
76-77	3.091757672372949E-4
78-79	2.1447646281677932E-4
80-81	1.3960837616750996E-5
82-83	2.837863708416996E-6
84-85	0.0
86-87	9.986434310145636E-5
88-89	4.973632109601115E-4
90-91	3.0654191718549393E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	943.0
20-21	3214.0
22-23	6566.0
24-25	12391.0
26-27	17424.0
28-29	28652.0
30-31	58799.0
32-33	76952.0
34-35	107820.0
36-37	146111.0
38-39	174182.0
40-41	179465.0
42-43	214572.0
44-45	255843.0
46-47	232338.0
48-49	217127.0
50-51	200879.0
52-53	198834.0
54-55	215993.0
56-57	228815.0
58-59	231017.0
60-61	230149.0
62-63	225330.0
64-65	238685.0
66-67	254595.0
68-69	261822.0
70-71	264849.0
72-73	256958.0
74-75	263943.0
76-77	245172.0
78-79	272078.0
80-81	287726.0
82-83	287918.0
84-85	300266.0
86-87	325772.0
88-89	365217.0
90-91	406995.0
92-93	459530.0
94-95	563299.0
96-97	1076244.0
98-99	1759010.0
100-101	1.2146615E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.3519337930898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.28235641591642	74.41857060870511
2	7.1854539911147235	11.978429706813746
3	1.851359901517334	4.629432838153622
4	0.7565888037891327	2.5225255952809915
5	0.329653641774095	1.3738634261902654
6	0.1860853706016409	0.930634529415033
7	0.11383825086263546	0.6642046844316476
8	0.07052816465518796	0.470292712870989
9	0.045811638048557354	0.3436639759418711
>10	0.17305662566691357	2.2381957113188142
>50	0.0040047460346109166	0.22089165682494818
>100	0.0011911966654962853	0.15669228628235604
>500	4.0809738621029724E-5	0.02353069161536053
>1k	3.0443614608602195E-5	0.029071576155243237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0042776622084616E-5	0.0	4.291825231726374E-6	0.0
3	0.0	3.0042776622084616E-5	0.0	8.583650463452748E-6	0.0
4	0.0	9.87119803297066E-5	0.0	8.583650463452748E-6	0.0
5	0.0	9.87119803297066E-5	0.0	8.583650463452748E-6	0.0
6	0.0	9.87119803297066E-5	0.0	1.2875475695179122E-5	8.583650463452748E-6
7	0.0	1.0300380556143298E-4	0.0	1.2875475695179122E-5	8.583650463452748E-6
8	0.0	1.158792812566121E-4	0.0	1.2875475695179122E-5	8.583650463452748E-6
9	0.0	2.746768148304879E-4	0.0	3.0042776622084616E-5	1.7167300926905495E-5
10-11	0.0	2.8326046529394066E-4	0.0	6.866920370762198E-5	1.7167300926905495E-5
12-13	0.0	2.8326046529394066E-4	0.0	8.15446794028011E-5	1.7167300926905495E-5
14-15	0.0	3.648051446967418E-4	0.0	1.008578929455698E-4	4.7210077548990115E-5
16-17	0.0	3.7982653300778406E-4	0.0	1.2017110648833846E-4	5.579372801244286E-5
18-19	0.0	4.270366105567742E-4	0.0	1.330465821835176E-4	5.579372801244286E-5
20-21	0.0	4.7639260072162746E-4	0.0	1.5235979572628626E-4	5.579372801244286E-5
22-23	0.0	5.27894503502344E-4	0.0	1.673811840373286E-4	7.081511632348517E-5
24-25	0.0	5.944177945941028E-4	0.0	1.8884031019596046E-4	8.583650463452748E-5
26-27	0.0	6.244605712161874E-4	0.0	2.446340382084033E-4	9.012832986625385E-5
28-29	0.0	6.287523964479138E-4	0.0	4.8283033856921705E-4	9.012832986625385E-5
30-31	0.0	6.995675127713989E-4	0.0	0.001057934919620551	9.012832986625385E-5
32-33	0.0	7.017134253872622E-4	0.0	0.0024227353433095384	9.87119803297066E-5
34-35	0.0	8.347600075707797E-4	0.0	0.004371223998513312	1.0300380556143298E-4
36-37	0.0	9.613688519067077E-4	0.0	0.007289665156087246	1.0300380556143298E-4
38-39	0.0	9.999952789922452E-4	0.0	0.01224886921134707	1.0300380556143298E-4
40-41	0.0	0.001090123608858499	0.0	0.017259575169387614	1.0729563079315935E-4
42-43	0.0	0.0011223122980964467	0.0	0.02215440184617154	1.0944154340902253E-4
44-45	0.0	0.0012746720938227331	0.0	0.02805351562717944	1.137333686407489E-4
46-47	0.0	0.0013690922489207132	0.0	0.03418438797070057	1.2231701910420166E-4
48-49	0.0	0.0013755299867683028	0.0	0.04043543142071003	1.330465821835176E-4
50-51	0.0	0.0015235979572628627	0.0	0.04683239692859819	1.330465821835176E-4
52-53	0.0	0.0015321816077263155	0.0	0.05291391328195447	1.4592205787869673E-4
54-55	0.0	0.0015472029960373578	0.0	0.0590619529264025	1.5879753357387583E-4
56-57	0.0	0.0015793916852753056	0.0	0.06543745930813202	1.5879753357387583E-4
58-59	0.0	0.0015944130735863478	0.0	0.07199536826220992	1.60943446189739E-4
60-61	0.0	0.0016137262871291165	0.0	0.07818847207159108	1.673811840373286E-4
62-63	0.0	0.0016437690637512012	0.0	0.0842850098132584	1.802566597325077E-4
64-65	0.0	0.001815442073020256	0.0	0.09027854374936428	1.802566597325077E-4
66-67	0.0	0.0018304634613312983	0.0	0.09654675450030065	1.802566597325077E-4
68-69	4.291825231726374E-6	0.0018905490145754676	0.0	0.10261539537796174	1.802566597325077E-4
70-71	4.291825231726374E-6	0.0019313213542768682	0.0	0.10808318072318113	1.8454848496423406E-4
72-73	4.291825231726374E-6	0.0019463427425879106	0.0	0.11238788143060269	2.1888308681804506E-4
74-75	4.291825231726374E-6	0.0020171578589113955	0.0	0.11586425986830105	2.1888308681804506E-4
76-77	4.291825231726374E-6	0.0021502044410949134	0.0	0.11904235645239444	2.1888308681804506E-4
78-79	4.291825231726374E-6	0.002180247217716998	0.0	0.12119899863133693	2.1888308681804506E-4
80-81	4.291825231726374E-6	0.002182393130332861	0.0	0.12191358753241938	2.29612649897361E-4
82-83	4.291825231726374E-6	0.0021909767807963137	0.0	0.12256594496764178	2.5107177605599286E-4
84-85	4.291825231726374E-6	0.0022081440817232193	0.0	0.1228213085689295	2.5536360128771926E-4
86-87	4.291825231726374E-6	0.0022660837223515253	0.0	0.12296508471419233	2.5750951390358245E-4
88	4.291825231726374E-6	0.00239054665407159	0.0	0.12297366836465579	2.618013391353088E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30325	0.0	50.086124	1
GTATCAA	53815	0.0	33.448112	1
TCAACGC	64600	0.0	27.165726	4
ATCAACG	65490	0.0	26.79655	3
CAACGCA	66445	0.0	26.392792	5
AACGCAG	67550	0.0	25.991573	6
TATCAAC	68565	0.0	25.819134	2
ACGCAGA	78020	0.0	22.461317	7
CGCAGAG	81510	0.0	21.47936	8
GTACATG	68090	0.0	20.900007	1
TACATGG	69875	0.0	20.058912	2
ACATGGG	69320	0.0	19.605635	3
GCAGAGT	91860	0.0	18.852968	9
GAGTACT	54575	0.0	18.488426	12-13
CAGAGTA	90575	0.0	17.743427	10-11
GTGGTAT	14325	0.0	17.695473	1
AGAGTAC	84125	0.0	17.577005	10-11
CATGGGG	48850	0.0	17.28692	4
TGGTATC	14080	0.0	16.156168	2
GTACTTT	62710	0.0	16.055038	14-15
>>END_MODULE