FastQCFastQC Report
Fri 27 May 2016
SRR2140184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10627919
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105400.047.014631
GTATCAA192150.034.0492671
TCAACGC244800.025.722974
ATCAACG244950.025.6283323
CAACGCA255300.024.8467065
TATCAAC258050.024.7665232
AACGCAG255900.024.4682736
ACGCAGA295800.020.9726927
CGCAGAG299200.020.4726038
GTACATG247800.019.7934631
TACATGG251050.019.117972
ACATGGG250750.018.2855113
AGAGTAC312650.017.76838310-11
GCAGAGT342800.017.495979
CAGAGTA341450.016.0681869
GAGTACT207350.015.90445112-13
GTGGTAT54150.015.5499381
CATGGGG183000.015.14028554
AGTACTT238850.015.0472412-13
GTACTTT243050.013.79394814-15
Produced by FastQC (version 0.11.5)