##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10627919
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.792820212498796	31.0	30.0	31.0	26.0	34.0
2	30.30865722631119	31.0	30.0	31.0	27.0	34.0
3	29.899924340785812	31.0	30.0	31.0	26.0	34.0
4	33.783902568320286	35.0	33.0	37.0	28.0	37.0
5	33.53753128905104	35.0	33.0	37.0	28.0	37.0
6	33.583254915661286	35.0	33.0	37.0	28.0	37.0
7	33.53551838323194	35.0	33.0	37.0	28.0	37.0
8	33.47025424262266	35.0	33.0	37.0	28.0	37.0
9	34.71181140917615	37.0	33.0	39.0	28.0	39.0
10-11	34.697456764583926	37.0	33.0	39.0	27.5	39.0
12-13	34.566623296620904	37.0	33.0	39.0	27.0	39.0
14-15	35.39439273106993	37.0	33.0	40.0	27.0	41.0
16-17	35.297658130439274	37.0	33.0	40.0	27.0	41.0
18-19	35.462682111145185	37.5	33.5	40.0	27.0	41.0
20-21	35.47403134646906	38.0	33.5	40.0	27.0	41.0
22-23	35.4319483681262	38.0	33.0	40.0	27.0	41.0
24-25	35.42325602876066	38.0	33.0	40.0	27.0	41.0
26-27	35.092030004503485	37.0	33.0	40.0	27.0	41.0
28-29	34.833231502511126	37.0	33.0	40.0	25.5	41.0
30-31	34.77328326083787	37.0	33.0	40.0	25.0	41.0
32-33	34.735296905458725	37.0	33.0	40.0	25.0	41.0
34-35	34.503009498343545	37.0	32.5	40.0	25.0	41.0
36-37	34.40500604661298	37.0	32.0	40.0	25.0	41.0
38-39	34.37192325169342	37.0	32.0	40.0	25.0	41.0
40-41	34.29433725520863	37.0	32.0	40.0	24.0	41.0
42-43	34.28876541841694	37.0	32.0	40.0	24.0	41.0
44-45	34.35902129424278	37.0	32.0	40.0	24.5	41.0
46-47	34.18609019315424	37.0	32.0	40.0	24.0	41.0
48-49	34.16889602926777	37.0	32.0	40.0	24.0	41.0
50-51	34.11095775407516	37.0	32.0	39.5	24.0	41.0
52-53	34.159494988871145	37.0	32.0	39.0	24.0	41.0
54-55	34.25092727474278	37.0	32.0	39.0	24.5	41.0
56-57	34.21065932266771	36.5	32.0	39.0	25.0	40.5
58-59	33.93542494936689	36.0	31.0	39.0	24.0	40.0
60-61	33.610248634686	36.0	31.0	39.0	23.5	40.0
62-63	33.50410842208714	35.5	31.0	39.0	23.5	40.0
64-65	33.308577688035996	35.0	31.0	39.0	23.5	40.0
66-67	33.051214280668724	35.0	31.0	38.0	23.0	40.0
68-69	32.88644960417279	35.0	30.0	38.0	23.0	40.0
70-71	32.866186849804755	35.0	30.5	38.0	24.0	40.0
72-73	32.663909441332976	34.0	30.0	37.0	24.0	40.0
74-75	32.3040985622831	34.0	30.0	37.0	23.0	39.0
76-77	31.37697361575545	33.5	28.5	36.0	22.0	39.0
78-79	31.81943432583602	34.0	30.0	36.0	24.0	39.0
80-81	31.844952949422176	34.0	30.0	36.0	24.5	37.5
82-83	31.868349792790262	34.0	30.0	35.0	25.0	37.0
84-85	31.612538850962473	34.0	30.0	35.0	25.0	37.0
86-87	31.433277497259038	34.0	30.0	35.0	24.5	36.0
88-89	31.39219509386406	34.0	30.0	35.0	25.0	36.0
90-91	31.283064829430586	33.0	30.0	35.0	25.0	36.0
92-93	31.22522362414849	33.0	30.0	35.0	25.0	35.0
94-95	31.43399156675877	33.5	30.0	35.0	25.0	35.0
96-97	31.669775615900377	34.0	30.5	35.0	25.5	35.0
98-99	32.1243456295619	34.0	31.0	35.0	26.5	35.0
100	31.708053920785147	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	22.0
19	218.0
20	1198.0
21	5723.0
22	20993.0
23	54720.0
24	114260.0
25	196677.0
26	295776.0
27	400623.0
28	502559.0
29	592304.0
30	674451.0
31	750673.0
32	824433.0
33	898393.0
34	965150.0
35	1022054.0
36	1067069.0
37	1066484.0
38	852011.0
39	313430.0
40	8695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.69944228969001	22.88783909625205	15.57115743919388	24.841561174864054
2	14.15846802199188	23.164099629766962	39.94511441085515	22.732317937386007
3	16.730003054378	27.758753046586214	31.90686688426113	23.604377014774656
4	12.251346340234859	20.192976379060433	39.05465379363339	28.501023487071315
5	12.166652756762636	39.4331759585296	36.113918444429245	12.286252840278514
6	28.103053641254878	40.55189820154533	18.80900858890118	12.536039568298616
7	24.36502844348521	34.10271269525345	24.437432095113277	17.09482676614806
8	23.23755948836268	38.16912793558174	21.346079133647894	17.24723344240768
9	24.259175673533985	17.279793058196454	22.28634516125025	36.17468610701932
10-11	23.17619504576049	27.76956326074322	29.912982132263316	19.141259561232975
12-13	23.703445950720674	25.244143496578065	30.02471710311938	21.02769344958188
14-15	21.010562886796695	26.909384937417425	27.261514096104523	24.81853807968136
16-17	20.570039453181472	29.888957037521998	28.011824710466453	21.52917879883008
18-19	20.671708166623542	29.10185182046528	29.638449257136273	20.58799075577491
20-21	21.35875025255008	28.15713248251233	29.506264065562824	20.977853199374763
22-23	21.07142376341032	28.12478788401995	29.493241331758384	21.31054702081135
24-25	20.99206798167669	28.28192074274462	29.38952162678677	21.336489648791922
26-27	21.13377516599615	28.263079437303556	29.27268802674859	21.330457369951702
28-29	21.122190638017262	28.174810381038117	29.315685826063177	21.387313154881447
30-31	21.389123344039252	28.170905360274052	29.16200950255478	21.277961793131915
32-33	21.241862305639298	28.32109585822077	29.131739313047174	21.305302523092756
34-35	21.2576277970892	28.195914001454682	29.19379543823587	21.35266276322025
36-37	21.262616870075586	28.23071217416217	29.147367531690122	21.359303424072117
38-39	21.248218158922736	28.284998018329656	29.109571385855837	21.357212436891775
40-41	21.426723747719482	28.227088679423417	28.964344891041094	21.381842681816007
42-43	21.293984173386175	28.371477612640582	28.811618401722676	21.522919812250564
44-45	21.46271631372379	28.47150811725829	28.516785609174605	21.548989959843322
46-47	21.46730102425584	28.37832422256535	28.418529282344174	21.73584547083464
48-49	21.398899952938972	28.51737801359107	28.415177985020645	21.66854404844931
50-51	21.374647304411003	28.565306674138835	28.378526249391328	21.681519772058834
52-53	21.52147624708118	28.50903802087038	28.230991723473814	21.738494008574627
54-55	21.43685675595397	28.434066896524413	28.35943259035176	21.769643757169863
56-57	21.494752728740497	28.528061950192463	28.30991166156317	21.66727365950387
58-59	21.43227853519941	28.52169408346843	28.360828995733144	21.685198385599016
60-61	21.46551465464734	28.47735299685967	28.339196151318944	21.717936197174048
62-63	21.541126276554788	28.61127795084515	28.226877031189655	21.620718741410407
64-65	21.395287869631993	28.540576220166525	28.33654713628036	21.727588773921124
66-67	21.45181767265449	28.51868700316544	28.241067585498463	21.788427738681612
68-69	21.328527990841753	28.484531356145776	28.375800855945993	21.81113979706648
70-71	21.41857457492248	28.256876000354424	28.38520279998849	21.939346624734604
72-73	21.493605199691263	28.420452151455635	28.287614551759987	21.798328097093115
74-75	21.26705655430692	28.53469494437924	28.34360020973396	21.854648291579878
76-77	21.3993715828376	28.439666021917258	28.40887285233917	21.752089542905974
78-79	21.31961192968391	28.473516673140647	28.2928917151498	21.913979682025648
80-81	21.444379054620875	28.492198566878514	28.255988097737745	21.807434280762873
82-83	21.399895598924406	28.50052231870653	28.363190411644794	21.736391670724274
84-85	21.437653969478582	28.48242094617625	28.361209845474345	21.718715238870825
86-87	21.337317503755795	28.48424490143949	28.403890792240567	21.774546802564156
88-89	21.360250801874695	28.465489787347238	28.34154499391791	21.832714416860156
90-91	21.28447339375362	28.482965271620593	28.48531176536042	21.747249569265367
92-93	21.22743924348594	28.45878872128913	28.529786117376172	21.783985917848764
94-95	21.070161014381103	28.530842695934023	28.60443459350135	21.79456169618352
96-97	21.111803844477922	28.6829067904408	28.62798417911183	21.577305185969443
98-99	22.133225988476244	30.730212225011	28.643430200912846	18.493131585599905
100	21.37759727945595	31.54582451130311	22.521239620665806	24.555338588575136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2273.0
1	2008.0
2	2914.0
3	4231.5
4	5023.0
5	7571.5
6	10366.5
7	12643.0
8	14260.0
9	14401.5
10	13387.0
11	11881.5
12	12042.0
13	14781.0
14	22664.0
15	34777.0
16	45290.5
17	51674.0
18	55040.5
19	56580.5
20	57341.5
21	58516.0
22	60820.5
23	67148.0
24	79432.5
25	98819.5
26	124535.0
27	153822.5
28	189646.0
29	232075.5
30	273319.0
31	311423.5
32	345200.5
33	372186.5
34	393851.0
35	409925.5
36	422605.0
37	430872.0
38	429960.0
39	422014.0
40	415160.5
41	409374.5
42	403541.0
43	400290.0
44	397320.0
45	396416.5
46	394862.0
47	391522.0
48	387490.5
49	380617.5
50	372893.0
51	362178.0
52	347077.5
53	330121.0
54	312206.5
55	292020.5
56	269884.5
57	245723.0
58	219321.0
59	190606.5
60	160704.5
61	132306.0
62	107001.5
63	84701.5
64	65134.5
65	50317.5
66	38807.5
67	29976.0
68	23454.5
69	17982.5
70	13745.5
71	10422.5
72	7886.5
73	5961.5
74	4409.5
75	3248.5
76	2384.0
77	1690.5
78	1242.0
79	932.0
80	687.0
81	509.5
82	372.0
83	251.0
84	167.0
85	115.0
86	90.5
87	70.0
88	53.5
89	45.0
90	37.0
91	32.0
92	25.5
93	15.5
94	6.5
95	4.0
96	4.0
97	4.0
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.020502602626158516
3	1.8843199689421795
4	0.4737992451767839
5	0.0
6	1.5995605536700083E-4
7	1.881835945494127E-5
8	0.0
9	0.021857524506914288
10-11	0.1436875836182041
12-13	0.004328222674636493
14-15	4.704589863735318E-6
16-17	5.1750488501088495E-5
18-19	0.07214018097051737
20-21	0.01897677493074395
22-23	6.684109367845178E-4
24-25	1.4130486184812065E-4
26-27	0.004314985042328353
28-29	0.0012561533213520234
30-31	8.138437471107365E-4
32-33	0.002007231157010746
34-35	0.027567212106597475
36-37	0.0
38-39	2.4090321746964607E-5
40-41	0.0011940958658022641
42-43	1.272053306861578E-4
44-45	6.073833220341929E-4
46-47	0.0013474701920878646
48-49	0.004185044094481757
50-51	0.00749468892840459
52-53	0.004589521756928547
54-55	0.018099954194869358
56-57	0.006959303910179564
58-59	0.008099356777408619
60-61	0.00896344904072578
62-63	0.007711537660516064
64-65	0.021221159595153905
66-67	0.0030437602580841603
68-69	0.0026001689715011893
70-71	0.001818988142656948
72-73	6.034080369889127E-4
74-75	1.3536520826937817E-4
76-77	3.583377642793269E-5
78-79	6.060833066353636E-6
80-81	0.0
82-83	3.7599412847568974E-5
84-85	5.102548467832259E-5
86-87	3.8994214428405255E-5
88-89	1.3273871697676423E-5
90-91	2.040428922644687E-5
92-93	0.0021998512481536964
94-95	1.0072201859587464E-4
96-97	1.1236200821770782E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1070.0
20-21	3334.0
22-23	5956.0
24-25	11579.0
26-27	13897.0
28-29	18090.0
30-31	28724.0
32-33	35893.0
34-35	49828.0
36-37	63421.0
38-39	76004.0
40-41	79107.0
42-43	90126.0
44-45	104730.0
46-47	105336.0
48-49	97277.0
50-51	91922.0
52-53	92013.0
54-55	99213.0
56-57	106774.0
58-59	110176.0
60-61	110008.0
62-63	106654.0
64-65	111572.0
66-67	119899.0
68-69	122920.0
70-71	124531.0
72-73	121842.0
74-75	125132.0
76-77	118610.0
78-79	133295.0
80-81	135781.0
82-83	139148.0
84-85	143901.0
86-87	153455.0
88-89	179691.0
90-91	187846.0
92-93	202913.0
94-95	244159.0
96-97	473975.0
98-99	819799.0
100-101	5468318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.43593555477747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.1174822638522	83.2804914277302
2	4.732016156893152	8.464245841041237
3	1.2054984001740467	3.2344463168806024
4	0.43924474179594325	1.5713705768014743
5	0.19121377561402328	0.8550691456500734
6	0.10432995647848152	0.5598508358425327
7	0.06649019946254849	0.4162629235909751
8	0.03710214383917518	0.26546119562756443
9	0.02666735988431735	0.21465182520268786
>10	0.07883209982092865	1.055740167089907
>50	9.476208108806079E-4	0.053808228899426665
>100	1.7528137428589265E-4	0.028601515643310045
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8818359454941274E-5	0.0	9.409179727470637E-6	0.0
3	0.0	2.822753918241191E-5	0.0	1.8818359454941274E-5	0.0
4	0.0	6.586425809229446E-5	0.0	3.763671890988255E-5	0.0
5	0.0	6.586425809229446E-5	0.0	3.763671890988255E-5	0.0
6	1.4113769591205956E-4	6.586425809229446E-5	0.0	6.586425809229446E-5	0.0
7	1.4113769591205956E-4	6.586425809229446E-5	0.0	1.1291015672964764E-4	0.0
8	1.4113769591205956E-4	9.409179727470637E-5	0.0	1.1291015672964764E-4	9.409179727470637E-6
9	1.4113769591205956E-4	1.8818359454941274E-4	0.0	1.505468756395302E-4	9.409179727470637E-6
10-11	1.4584228577579488E-4	1.8818359454941274E-4	0.0	1.8347900468567742E-4	9.409179727470637E-6
12-13	1.505468756395302E-4	1.8818359454941274E-4	0.0	2.305249033230306E-4	9.409179727470637E-6
14-15	1.505468756395302E-4	2.587524425054425E-4	0.0	2.728662120966485E-4	2.822753918241191E-5
16-17	2.4934326277797187E-4	2.6345703236917785E-4	0.0	2.822753918241191E-4	2.822753918241191E-5
18-19	2.5404785264170716E-4	2.7757080196038383E-4	0.0	2.9168457155158976E-4	3.763671890988255E-5
20-21	2.5404785264170716E-4	3.10502931006531E-4	0.0	3.1520752087026637E-4	4.7045898637353184E-5
22-23	2.6816162223291314E-4	3.575488296438842E-4	0.0	3.293212904614723E-4	5.17504885010885E-5
24-25	2.869799816878544E-4	3.8107177896256077E-4	0.0	3.434350600526782E-4	5.645507836482382E-5
26-27	2.9168457155158976E-4	3.9518554855376675E-4	0.0	3.716625992350902E-4	5.645507836482382E-5
28-29	3.1991211073400166E-4	4.045947282812374E-4	0.0	6.915747099690918E-4	5.645507836482382E-5
30-31	3.293212904614723E-4	4.422314471911199E-4	0.0	0.002022973641406187	5.645507836482382E-5
32-33	3.3873047018894293E-4	4.422314471911199E-4	0.0	0.005010388204878114	5.645507836482382E-5
34-35	3.575488296438842E-4	5.080957052834144E-4	0.0	0.008242441441264278	6.586425809229446E-5
36-37	3.575488296438842E-4	5.880737329669147E-4	0.0	0.013737402402107128	6.586425809229446E-5
38-39	3.575488296438842E-4	6.115966822855914E-4	0.0	0.02286901132761738	7.52734378197651E-5
40-41	3.575488296438842E-4	6.727563505141505E-4	0.0	0.032320532363861634	7.52734378197651E-5
42-43	3.575488296438842E-4	6.962792998328271E-4	0.0	0.040233652514664445	7.997802768350042E-5
44-45	3.622534195076195E-4	7.903710971075335E-4	0.0	0.0498451296062757	9.409179727470637E-5
46-47	3.6695800937135484E-4	8.844628943822398E-4	0.0	0.06011995386867364	1.0820556686591232E-4
48-49	3.716625992350902E-4	8.938720741097104E-4	0.0	0.0688657864253576	1.1291015672964764E-4
50-51	4.187084978724433E-4	9.409179727470637E-4	0.0	0.07886774447565888	1.1291015672964764E-4
52-53	4.328222674636493E-4	9.409179727470637E-4	0.0	0.08840394812945036	1.270239263208536E-4
54-55	4.375268573273846E-4	9.45622562610799E-4	0.0	0.09791192424405945	1.4584228577579488E-4
56-57	4.422314471911199E-4	9.503271524745342E-4	0.0	0.1068035990865192	1.5995605536700083E-4
58-59	4.422314471911199E-4	9.503271524745342E-4	0.0	0.11493783496091756	1.9759277427688337E-4
60-61	4.5164062691859056E-4	9.644409220657402E-4	0.0	0.12315675345286317	1.9759277427688337E-4
62-63	4.89277345828473E-4	9.92668461248152E-4	0.0	0.1327305938255645	2.07001954004354E-4
64-65	4.89277345828473E-4	0.001110283207841535	0.0	0.14164579161734298	2.07001954004354E-4
66-67	5.080957052834143E-4	0.0011243969774327412	0.0	0.14845333315016795	2.1170654386808933E-4
68-69	5.17504885010885E-4	0.001166738286206359	0.0	0.15611240544832905	2.1641113373182464E-4
70-71	5.269140647383556E-4	0.0011902612355250354	0.0	0.1620731208056817	2.1641113373182464E-4
72-73	5.786645532394442E-4	0.0011996704152525063	0.0	0.16771862864216408	2.3522949318676592E-4
74-75	5.833691431031795E-4	0.0012231933645711827	0.0	0.17208448803571047	2.3522949318676592E-4
76-77	5.927783228306501E-4	0.0012796484429360064	0.0	0.17602693434152067	2.3522949318676592E-4
78-79	5.927783228306501E-4	0.0013078759821184185	0.0	0.17830395583556857	2.3522949318676592E-4
80-81	5.927783228306501E-4	0.0013125805719821537	0.0	0.17908962234281237	2.446386729142365E-4
82-83	5.927783228306501E-4	0.0013266943415733597	0.0	0.18019049637092643	2.6345703236917785E-4
84-85	5.927783228306501E-4	0.0013502172908920365	0.0	0.1805997956890714	2.6345703236917785E-4
86-87	5.927783228306501E-4	0.0013549218807557717	0.0	0.18074093338498345	2.6345703236917785E-4
88	6.21005862013062E-4	0.0014113769591205954	0.0	0.18075034256471093	2.6345703236917785E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10540	0.0	47.01463	1
GTATCAA	19215	0.0	34.049267	1
TCAACGC	24480	0.0	25.72297	4
ATCAACG	24495	0.0	25.628332	3
CAACGCA	25530	0.0	24.846706	5
TATCAAC	25805	0.0	24.766523	2
AACGCAG	25590	0.0	24.468273	6
ACGCAGA	29580	0.0	20.972692	7
CGCAGAG	29920	0.0	20.472603	8
GTACATG	24780	0.0	19.793463	1
TACATGG	25105	0.0	19.11797	2
ACATGGG	25075	0.0	18.285511	3
AGAGTAC	31265	0.0	17.768383	10-11
GCAGAGT	34280	0.0	17.49597	9
CAGAGTA	34145	0.0	16.068186	9
GAGTACT	20735	0.0	15.904451	12-13
GTGGTAT	5415	0.0	15.549938	1
CATGGGG	18300	0.0	15.1402855	4
AGTACTT	23885	0.0	15.04724	12-13
GTACTTT	24305	0.0	13.793948	14-15
>>END_MODULE