FastQCFastQC Report
Fri 27 May 2016
SRR2140183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10224735
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107750.047.58511
GTATCAA186850.032.652391
ATCAACG232300.025.297263
TCAACGC232500.025.2272554
CAACGCA240200.024.5445635
TATCAAC249100.023.8806822
AACGCAG246850.023.481756
ACGCAGA284900.020.2874187
CGCAGAG288400.019.7392988
GTACATG228500.018.7701341
TACATGG237550.017.3777562
AGAGTAC303050.017.36627610-11
TATACCG8650.017.2550055
GTGGTAT54700.017.1060241
ACATGGG237000.016.7209423
GCAGAGT329850.016.5925779
GAGTACT207500.016.33466312-13
CAGAGTA331050.016.0064019
TGGTATC52700.015.4253382
AGTACTT238800.014.98218212-13
Produced by FastQC (version 0.11.5)