##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10224735
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.81523971036902	31.0	30.0	31.0	26.0	34.0
2	30.30992451149101	31.0	30.0	31.0	27.0	34.0
3	29.890586601999953	31.0	30.0	31.0	26.0	34.0
4	33.77616759749764	35.0	33.0	37.0	28.0	37.0
5	33.52829075765778	35.0	33.0	37.0	28.0	37.0
6	33.578824194465675	35.0	33.0	37.0	28.0	37.0
7	33.53494716488985	35.0	33.0	37.0	28.0	37.0
8	33.46813858745483	35.0	33.0	37.0	28.0	37.0
9	34.70775389288818	37.0	33.0	39.0	28.0	39.0
10-11	34.70421096488075	37.0	33.0	39.0	27.5	39.0
12-13	34.56485263432255	37.0	33.0	39.0	27.0	39.0
14-15	35.39982072885019	37.0	33.0	40.0	27.0	41.0
16-17	35.30351495662235	37.0	33.0	40.0	27.0	41.0
18-19	35.46667361061191	37.5	33.5	40.0	27.0	41.0
20-21	35.485635976838665	38.0	33.5	40.0	27.0	41.0
22-23	35.43344090319509	38.0	33.0	40.0	27.0	41.0
24-25	35.423837397639055	38.0	33.0	40.0	27.0	41.0
26-27	35.12093713107395	37.0	33.0	40.0	27.0	41.0
28-29	34.875283032030254	37.0	33.0	40.0	26.0	41.0
30-31	34.8207668573321	37.0	33.0	40.0	25.5	41.0
32-33	34.77347887742385	37.0	33.0	40.0	25.5	41.0
34-35	34.54366241346018	37.0	33.0	40.0	25.0	41.0
36-37	34.43928397244912	37.0	32.5	40.0	25.0	41.0
38-39	34.41352419832863	37.0	32.0	40.0	25.0	41.0
40-41	34.325770678592654	37.0	32.0	40.0	24.5	41.0
42-43	34.326681319604155	37.0	32.0	40.0	24.5	41.0
44-45	34.38293483277586	37.0	32.0	40.0	24.5	41.0
46-47	34.21786269497761	37.0	32.0	40.0	24.0	41.0
48-49	34.201667266878985	37.0	32.0	40.0	24.0	41.0
50-51	34.13820765842004	37.0	32.0	39.5	24.0	41.0
52-53	34.1915271675235	37.0	32.0	39.0	24.0	41.0
54-55	34.28724074370228	37.0	32.0	39.0	24.5	41.0
56-57	34.242556851657525	36.5	32.0	39.0	25.0	41.0
58-59	33.974902065792485	36.0	31.0	39.0	24.0	40.0
60-61	33.651897438893634	36.0	31.0	39.0	23.5	40.0
62-63	33.542176643645334	36.0	31.0	39.0	23.5	40.0
64-65	33.34541706612241	35.0	31.0	39.0	23.5	40.0
66-67	33.1001136740845	35.0	31.0	38.0	23.0	40.0
68-69	32.930447060855116	35.0	30.0	38.0	23.0	40.0
70-71	32.90757399156263	35.0	30.5	38.0	24.0	40.0
72-73	32.714375572594236	34.0	30.0	37.0	24.0	40.0
74-75	32.35348925948836	34.0	30.0	37.0	23.0	39.0
76-77	31.423356770594136	33.5	28.5	36.0	22.5	39.0
78-79	31.87353292251963	34.0	30.0	36.0	24.0	39.0
80-81	31.893141670079576	34.0	30.0	36.0	24.5	38.0
82-83	31.913839753193397	34.0	30.0	35.0	25.0	37.0
84-85	31.650550991388673	34.0	30.0	35.0	25.0	37.0
86-87	31.462642651647396	34.0	30.0	35.0	24.5	36.0
88-89	31.420058946024856	34.0	30.0	35.0	25.0	36.0
90-91	31.312908562907168	33.5	30.0	35.0	25.0	36.0
92-93	31.254270190990503	33.0	30.0	35.0	25.0	35.5
94-95	31.44656428483619	33.5	30.0	35.0	25.0	35.0
96-97	31.68203926535289	34.0	30.5	35.0	25.5	35.0
98-99	32.12887168437125	34.0	31.0	35.0	26.5	35.0
100	31.708498110981143	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	16.0
19	157.0
20	1084.0
21	5313.0
22	19288.0
23	51744.0
24	107391.0
25	186676.0
26	281780.0
27	381555.0
28	479023.0
29	566297.0
30	645746.0
31	721535.0
32	794882.0
33	864157.0
34	929027.0
35	987529.0
36	1031098.0
37	1029584.0
38	827437.0
39	305557.0
40	7858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.58329531278805	23.088715746667273	15.526064978701159	24.80192396184351
2	14.21015711762903	23.352466852520607	39.77709265916723	22.660283370683135
3	16.691035544943166	27.878179460917423	32.088326328962616	23.342458665176796
4	12.309243700121437	20.28299411603203	39.115288156025876	28.292474027820656
5	12.10046622257996	39.45142805726899	36.2166335932587	12.231472126892347
6	28.021168305831356	40.58692071763335	18.893358448935523	12.498552527599777
7	24.13481620795062	34.134361428438	24.70874795288093	17.02207441073045
8	23.17826329973344	38.1462795857301	21.454785869756037	17.220671244780426
9	24.136735909874695	17.227884150820437	22.427429431374723	36.20795050793015
10-11	23.0020155577671	27.774668104853667	30.102833700470622	19.120482636908616
12-13	23.568663876980473	25.354937752388224	30.121940532424237	20.95445783820707
14-15	20.93835320084673	27.031270844067112	27.334320351868392	24.69605560321777
16-17	20.456813018201313	29.95416847417152	28.166967334738064	21.422051172889102
18-19	20.55457680354639	29.21374679693951	29.7691682400076	20.462508159506505
20-21	21.226326579371108	28.294329884702783	29.66246071102983	20.816882824896275
22-23	20.966082374688792	28.233863962548796	29.60926091678583	21.190792745976587
24-25	20.907985746319817	28.340072310187715	29.522084215931077	21.22985772756139
26-27	21.006312819691498	28.404552099140457	29.424222541841566	21.164912539326476
28-29	20.965224741281013	28.29192818744255	29.534342625165642	21.208504446110798
30-31	21.25820922646196	28.271557669676106	29.40130374335762	21.068929360504313
32-33	21.16311072212076	28.434169421070276	29.290510089474186	21.11220976733478
34-35	21.159123196421483	28.352993232441477	29.319661493927374	21.16822207720967
36-37	21.174851341739284	28.360501812032734	29.248812896125862	21.21583395010212
38-39	21.16046240366965	28.423457716675575	29.218352187038843	21.19772769261593
40-41	21.334461907937765	28.342911208758398	29.083802708155986	21.23882417514785
42-43	21.17686896635576	28.52618142738994	28.94431009986921	21.352639506385092
44-45	21.339853295225414	28.5629432459801	28.698357366506656	21.398846092287833
46-47	21.329661109881172	28.526529391926143	28.583169705760035	21.56063979243265
48-49	21.30141407697484	28.64419673969979	28.54948647761565	21.50490270570972
50-51	21.278300289911677	28.718960715114417	28.490561750206716	21.512177244767187
52-53	21.394538409122692	28.644712457021242	28.37548539670448	21.585263737151582
54-55	21.351291714985184	28.586581411330155	28.47609844092256	21.5860284327621
56-57	21.3854148449119	28.659939561802734	28.44333275037181	21.51131284291356
58-59	21.313985812371055	28.644851018493085	28.488169474389334	21.552993694746526
60-61	21.36831863710166	28.59554236114207	28.49540328309358	21.540735718662695
62-63	21.396622599494812	28.72041791919719	28.393790999362096	21.4891684819459
64-65	21.252199414820126	28.705156265346567	28.48916732202064	21.553476997812666
66-67	21.305993796214405	28.68565334266237	28.410212653916027	21.598140207207198
68-69	21.20507231908426	28.643575136528938	28.521270931113225	21.630081613273582
70-71	21.264137808006744	28.473960950634236	28.57377578901134	21.68812545234768
72-73	21.348395357585773	28.58990129794779	28.38082024555523	21.680883098911206
74-75	21.155666965718396	28.680868110917746	28.465834328893752	21.697630594470102
76-77	21.281554435913677	28.598110343226008	28.525615545342113	21.5947196755182
78-79	21.22227474756414	28.570812453471184	28.46150849804287	21.745404300921802
80-81	21.256812516678643	28.65383999168142	28.420810790648993	21.66853670099095
82-83	21.279874394825303	28.646341320525604	28.49336495093902	21.580419333710076
84-85	21.288464509935604	28.64014277102787	28.529472430109383	21.541920288927145
86-87	21.203033643495182	28.635936561072334	28.54397481433021	21.617054981102275
88-89	21.203864291062416	28.643752393013973	28.514701601798937	21.637681714124675
90-91	21.129519408409948	28.6904073068268	28.612499700087103	21.567573584676154
92-93	21.169501708011033	28.576349199157104	28.71533648100122	21.538812611830647
94-95	20.94753935834188	28.695988822373884	28.749067511641275	21.607404307642962
96-97	20.989289373709713	28.856209646511143	28.767019608005523	21.38748137177362
98-99	22.021897086521243	30.82556912186785	28.82263765362611	18.329896137984797
100	21.226840031329612	31.75260060997525	22.66139278616045	24.359166572534683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2207.0
1	1916.0
2	2718.5
3	3942.5
4	4674.5
5	6984.0
6	9583.5
7	11854.0
8	13596.0
9	13916.5
10	12918.5
11	11457.5
12	11707.0
13	14534.5
14	22254.5
15	33739.0
16	43750.5
17	50090.5
18	53464.0
19	55373.0
20	56545.0
21	57938.5
22	60472.0
23	67034.0
24	79425.0
25	98049.5
26	122631.0
27	150705.5
28	183970.0
29	223196.0
30	261925.0
31	297732.0
32	331044.0
33	357301.0
34	377477.0
35	392745.5
36	405703.5
37	415960.5
38	417955.5
39	414903.0
40	410904.5
41	405055.5
42	398063.0
43	391020.5
44	384321.5
45	381095.5
46	377213.5
47	371533.0
48	365841.0
49	357673.5
50	349280.0
51	338276.0
52	323753.5
53	307712.5
54	289435.5
55	269001.5
56	247735.5
57	224942.0
58	200105.0
59	174374.0
60	147647.5
61	121797.0
62	98598.0
63	78010.0
64	60238.0
65	46356.5
66	35643.5
67	27719.0
68	21614.0
69	16498.0
70	12555.5
71	9556.5
72	7236.5
73	5328.0
74	3862.0
75	2864.5
76	2102.0
77	1520.5
78	1097.0
79	797.5
80	562.0
81	402.0
82	308.5
83	215.5
84	159.0
85	122.0
86	93.5
87	79.5
88	55.5
89	31.5
90	17.0
91	12.0
92	10.5
93	6.5
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.019677771599948556
3	1.8656913846666932
4	0.4720806945118871
5	4.890102286269522E-5
6	1.4670306858808566E-4
7	0.0
8	0.0
9	0.021203483513264648
10-11	0.14335823862427732
12-13	0.003980543261023391
14-15	2.9340613717617132E-5
16-17	4.40109205764257E-5
18-19	0.07196763534702855
20-21	0.019465811188283107
22-23	7.485723184708458E-4
24-25	1.6155502488265596E-4
26-27	0.002386633090447268
28-29	0.001059860010156992
30-31	8.651370748250665E-4
32-33	0.002060246029849219
34-35	0.02694303983177492
36-37	4.970601131080169E-6
38-39	4.5012407670174286E-5
40-41	0.0012238498417834122
42-43	1.065643919144014E-4
44-45	5.375985411725986E-4
46-47	0.001381073509991084
48-49	0.0035469380574020995
50-51	0.007554732003658325
52-53	0.0051856643346601195
54-55	0.018400599296998343
56-57	0.0069245611091704505
58-59	0.008107530300455534
60-61	0.00924735818278102
62-63	0.007837220811622138
64-65	0.020892485004326757
66-67	0.0033628388971196827
68-69	0.0027559113717508152
70-71	0.0018563553415357917
72-73	6.810411350637792E-4
74-75	2.2410658848536714E-4
76-77	1.1058516261671034E-4
78-79	2.4929622118657647E-5
80-81	6.3326590106930116E-6
82-83	3.862712999651583E-5
84-85	2.6200357936189944E-5
86-87	6.671629469441335E-6
88-89	2.7241702688143118E-5
90-91	2.789886660854403E-5
92-93	0.0020399941935323167
94-95	1.179197482413375E-4
96-97	8.435126781872605E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1049.0
20-21	3073.0
22-23	5350.0
24-25	9853.0
26-27	11681.0
28-29	15859.0
30-31	25791.0
32-33	33135.0
34-35	46258.0
36-37	58534.0
38-39	68792.0
40-41	72540.0
42-43	83503.0
44-45	97436.0
46-47	97767.0
48-49	88827.0
50-51	83685.0
52-53	84433.0
54-55	91877.0
56-57	98883.0
58-59	102069.0
60-61	101033.0
62-63	98920.0
64-65	104053.0
66-67	111897.0
68-69	115038.0
70-71	115719.0
72-73	114043.0
74-75	117870.0
76-77	112499.0
78-79	125909.0
80-81	128015.0
82-83	132630.0
84-85	136989.0
86-87	146782.0
88-89	170588.0
90-91	179546.0
92-93	194268.0
94-95	234147.0
96-97	458393.0
98-99	790046.0
100-101	5355955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.49204940335417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.70593983012954	84.79642536495815
2	4.452743126493248	8.058757019661455
3	1.0453222940651348	2.837800700309091
4	0.3575796400150505	1.2943245779950219
5	0.16529622371323796	0.7478997021223106
6	0.09328199948120017	0.5064767583297854
7	0.052629014984077606	0.3333755196792312
8	0.03639758463090337	0.26349536212659774
9	0.0228786106139481	0.1863299125761745
>10	0.0671478277180526	0.9164703269793079
>50	6.642372247858426E-4	0.03961362677270512
>100	1.1961093080827233E-4	0.019031128490143254
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9560409145078087E-5
2	0.0	9.780204572539043E-6	0.0	0.0	1.9560409145078087E-5
3	0.0	9.780204572539043E-6	0.0	0.0	1.9560409145078087E-5
4	0.0	6.846143200777331E-5	0.0	0.0	1.9560409145078087E-5
5	0.0	7.824163658031235E-5	0.0	0.0	1.9560409145078087E-5
6	3.912081829015617E-5	7.824163658031235E-5	0.0	9.780204572539043E-6	1.9560409145078087E-5
7	3.912081829015617E-5	7.824163658031235E-5	0.0	1.9560409145078087E-5	1.9560409145078087E-5
8	3.912081829015617E-5	7.824163658031235E-5	0.0	1.9560409145078087E-5	1.9560409145078087E-5
9	3.912081829015617E-5	1.1736245487046853E-4	0.0	2.9340613717617132E-5	2.9340613717617132E-5
10-11	5.8681227435234263E-5	1.1736245487046853E-4	0.0	5.8681227435234263E-5	3.423071600388665E-5
12-13	5.8681227435234263E-5	1.1736245487046853E-4	0.0	6.84614320077733E-5	3.912081829015617E-5
14-15	5.8681227435234263E-5	1.760436823057028E-4	0.0	8.313173886658186E-5	3.912081829015617E-5
16-17	1.0758225029792948E-4	1.760436823057028E-4	0.0	8.80218411528514E-5	3.912081829015617E-5
18-19	1.1736245487046853E-4	1.8582388687824185E-4	0.0	8.80218411528514E-5	3.912081829015617E-5
20-21	1.320327617292771E-4	2.1027439830958945E-4	0.0	1.0269214801165996E-4	4.40109205764257E-5
22-23	1.4670306858808566E-4	2.445051143134761E-4	0.0	1.4670306858808566E-4	4.890102286269522E-5
24-25	1.7115358001943326E-4	2.934061371761713E-4	0.0	1.6626347773316373E-4	4.890102286269522E-5
26-27	1.760436823057028E-4	3.129665463212494E-4	0.0	2.1027439830958945E-4	5.8681227435234263E-5
28-29	1.8582388687824183E-4	3.129665463212494E-4	9.780204572539043E-6	4.2543889890544844E-4	5.8681227435234263E-5
30-31	1.8582388687824183E-4	3.7164777375648365E-4	9.780204572539043E-6	0.0013447781287241184	5.8681227435234263E-5
32-33	1.9071398916451136E-4	3.7164777375648365E-4	9.780204572539043E-6	0.003447522111820013	6.846143200777331E-5
34-35	1.956040914507809E-4	4.1076859204663984E-4	9.780204572539043E-6	0.006435374608730691	1.0758225029792948E-4
36-37	1.956040914507809E-4	4.4988941033679604E-4	9.780204572539043E-6	0.010728884416075331	1.12472352584199E-4
38-39	1.956040914507809E-4	4.5966961490933505E-4	9.780204572539043E-6	0.01788310406088764	1.2225255715673806E-4
40-41	1.956040914507809E-4	5.085706377720302E-4	9.780204572539043E-6	0.025208477285719386	1.2714265944300756E-4
42-43	1.956040914507809E-4	5.232409446308388E-4	9.780204572539043E-6	0.03221110375965734	1.3692286401554662E-4
44-45	2.004941937370504E-4	5.965924789248817E-4	9.780204572539043E-6	0.04049004693031164	1.4670306858808566E-4
46-47	2.0538429602331992E-4	6.357132972150378E-4	9.780204572539043E-6	0.04925800032959289	1.4670306858808566E-4
48-49	2.0538429602331992E-4	6.454935017875769E-4	9.780204572539043E-6	0.05700392235104382	1.4670306858808566E-4
50-51	2.0538429602331992E-4	7.188450360816198E-4	9.780204572539043E-6	0.06452489966732634	1.564832731606247E-4
52-53	2.0538429602331992E-4	7.237351383678893E-4	9.780204572539043E-6	0.07328796296432133	1.6626347773316373E-4
54-55	2.1027439830958945E-4	7.286252406541588E-4	9.780204572539043E-6	0.08199234503388107	1.760436823057028E-4
56-57	2.1516450059585896E-4	7.432955475129673E-4	9.780204572539043E-6	0.08983117899877112	1.760436823057028E-4
58-59	2.1516450059585896E-4	7.432955475129673E-4	9.780204572539043E-6	0.09704407987101867	1.760436823057028E-4
60-61	2.2494470516839802E-4	7.481856497992368E-4	9.780204572539043E-6	0.10461884831245016	1.760436823057028E-4
62-63	2.2494470516839802E-4	7.775262635168539E-4	9.780204572539043E-6	0.11286356076710057	1.760436823057028E-4
64-65	2.2494470516839802E-4	8.606580023834358E-4	9.780204572539043E-6	0.12030140634451651	1.760436823057028E-4
66-67	2.2494470516839802E-4	8.606580023834358E-4	9.780204572539043E-6	0.12733826353445835	1.760436823057028E-4
68-69	2.2494470516839802E-4	8.89998616101053E-4	9.780204572539043E-6	0.134825010134737	1.8582388687824183E-4
70-71	2.2494470516839802E-4	8.997788206735921E-4	9.780204572539043E-6	0.14103054993601302	1.8582388687824183E-4
72-73	2.2494470516839802E-4	9.046689229598615E-4	9.780204572539043E-6	0.14606246518858435	1.8582388687824183E-4
74-75	2.2494470516839802E-4	9.340095366774787E-4	9.780204572539043E-6	0.1501114698816155	1.8582388687824183E-4
76-77	2.3472490974093705E-4	9.731303549676349E-4	9.780204572539043E-6	0.15403822201748996	1.8582388687824183E-4
78-79	2.445051143134761E-4	9.926907641127128E-4	9.780204572539043E-6	0.15644904244462082	1.8582388687824183E-4
80-81	2.445051143134761E-4	9.975808663989824E-4	9.780204572539043E-6	0.1572070082989926	1.8582388687824183E-4
82-83	2.445051143134761E-4	9.975808663989824E-4	9.780204572539043E-6	0.1578769523122115	1.9071398916451136E-4
84-85	2.445051143134761E-4	9.975808663989824E-4	9.780204572539043E-6	0.15820947926767784	1.956040914507809E-4
86-87	2.445051143134761E-4	0.0010269214801165996	9.780204572539043E-6	0.15838552294998354	1.956040914507809E-4
88	2.445051143134761E-4	0.0010660422984067558	9.780204572539043E-6	0.15840019325684235	1.956040914507809E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10775	0.0	47.5851	1
GTATCAA	18685	0.0	32.65239	1
ATCAACG	23230	0.0	25.29726	3
TCAACGC	23250	0.0	25.227255	4
CAACGCA	24020	0.0	24.544563	5
TATCAAC	24910	0.0	23.880682	2
AACGCAG	24685	0.0	23.48175	6
ACGCAGA	28490	0.0	20.287418	7
CGCAGAG	28840	0.0	19.739298	8
GTACATG	22850	0.0	18.770134	1
TACATGG	23755	0.0	17.377756	2
AGAGTAC	30305	0.0	17.366276	10-11
TATACCG	865	0.0	17.255005	5
GTGGTAT	5470	0.0	17.106024	1
ACATGGG	23700	0.0	16.720942	3
GCAGAGT	32985	0.0	16.592577	9
GAGTACT	20750	0.0	16.334663	12-13
CAGAGTA	33105	0.0	16.006401	9
TGGTATC	5270	0.0	15.425338	2
AGTACTT	23880	0.0	14.982182	12-13
>>END_MODULE