FastQCFastQC Report
Fri 27 May 2016
SRR2140182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9332913
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83600.042.0637441
GTATCAA149200.031.2390271
ATCAACG171700.025.977843
TCAACGC173050.025.8106564
CAACGCA177200.025.3930475
AACGCAG182450.024.4405966
TATCAAC193000.023.348892
ACGCAGA210750.021.042187
CGCAGAG214700.020.3885358
GTACATG200600.018.2587381
TACATGG205750.017.0506732
AGAGTAC229300.016.6356910-11
ACATGGG203200.016.6069473
GCAGAGT254450.016.4324289
GAGTACT148950.015.14620712-13
CAGAGTA254200.014.65249710-11
CATGGGG132000.013.863714
TATACTG65200.013.2886095
AGTACTT179700.013.16737312-13
TATACAG85800.013.1465875
Produced by FastQC (version 0.11.5)