##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9332913
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.777632235508893	31.0	30.0	31.0	26.0	34.0
2	30.29143580359101	31.0	30.0	31.0	27.0	34.0
3	29.8507631004382	31.0	30.0	31.0	26.0	34.0
4	33.733346383921074	35.0	33.0	37.0	28.0	37.0
5	33.499928800364906	35.0	33.0	37.0	28.0	37.0
6	33.54267193961842	35.0	33.0	37.0	28.0	37.0
7	33.500414929400925	35.0	33.0	37.0	28.0	37.0
8	33.443834738414466	35.0	33.0	37.0	28.0	37.0
9	34.66859328914777	37.0	33.0	39.0	28.0	39.0
10-11	34.67068716916144	37.0	33.0	39.0	27.5	39.0
12-13	34.55177724253939	37.0	33.0	39.0	27.0	39.0
14-15	35.37091661520899	37.0	33.0	40.0	27.0	41.0
16-17	35.26760417674525	37.0	33.0	40.0	27.0	41.0
18-19	35.4396228165847	37.5	33.5	40.0	27.0	41.0
20-21	35.447955917849	38.0	33.0	40.0	27.0	41.0
22-23	35.42007234259039	38.0	33.0	40.0	27.0	41.0
24-25	35.4042006409303	37.0	33.0	40.0	27.0	41.0
26-27	35.10788187082677	37.0	33.0	40.0	27.0	41.0
28-29	34.86918641875522	37.0	33.0	40.0	26.0	41.0
30-31	34.82495965718938	37.0	33.0	40.0	25.5	41.0
32-33	34.78277186036693	37.0	33.0	40.0	25.5	41.0
34-35	34.56295762671557	37.0	33.0	40.0	25.0	41.0
36-37	34.459438656775006	37.0	32.5	40.0	25.0	41.0
38-39	34.412394603011776	37.0	32.0	40.0	25.0	41.0
40-41	34.33406354492284	37.0	32.0	40.0	25.0	41.0
42-43	34.341226441108276	37.0	32.0	40.0	24.5	41.0
44-45	34.40801984228885	37.0	32.5	40.0	24.5	41.0
46-47	34.24903374739088	37.0	32.0	40.0	24.0	41.0
48-49	34.23533267698555	37.0	32.0	40.0	24.0	41.0
50-51	34.191827339805904	37.0	32.0	40.0	24.0	41.0
52-53	34.25558835949104	37.0	32.0	39.5	24.0	41.0
54-55	34.35938939431908	37.0	32.0	40.0	24.5	41.0
56-57	34.33357331553046	37.0	32.0	39.5	25.5	41.0
58-59	34.075875972074684	36.0	31.5	39.0	24.5	40.0
60-61	33.76780529441379	36.0	31.0	39.0	24.0	40.0
62-63	33.687868408960824	36.0	31.0	39.0	23.5	40.0
64-65	33.50055800445587	35.5	31.0	39.0	23.5	40.0
66-67	33.272991865402886	35.0	31.0	39.0	23.0	40.0
68-69	33.11497736555961	35.0	31.0	38.0	23.5	40.0
70-71	33.0908921380505	35.0	31.0	38.0	24.0	40.0
72-73	32.884017336174	35.0	30.5	38.0	24.0	40.0
74-75	32.5195681787435	34.0	30.0	37.0	23.5	39.5
76-77	31.578840354771625	33.5	28.5	36.0	22.5	39.0
78-79	32.013963232342185	34.0	30.0	36.0	24.0	39.0
80-81	32.022448118385206	34.0	30.0	36.0	24.5	38.5
82-83	32.03381634259007	34.0	30.0	36.0	25.0	37.0
84-85	31.748548885259567	34.0	30.0	35.0	25.0	37.0
86-87	31.57154109915504	34.0	30.0	35.0	25.0	36.5
88-89	31.504276999457232	34.0	30.0	35.0	25.0	36.0
90-91	31.368106940788802	34.0	30.0	35.0	25.0	36.0
92-93	31.308685642897977	34.0	30.0	35.0	25.0	35.5
94-95	31.497056988777537	34.0	30.0	35.0	25.0	35.0
96-97	31.71949335192995	34.0	30.5	35.0	25.5	35.0
98-99	32.152517838721025	34.0	31.0	35.0	26.5	35.0
100	31.725914326050216	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	27.0
19	131.0
20	953.0
21	4692.0
22	17079.0
23	44599.0
24	93382.0
25	163630.0
26	246522.0
27	338628.0
28	426261.0
29	509525.0
30	586030.0
31	656658.0
32	724321.0
33	786551.0
34	849230.0
35	898425.0
36	936123.0
37	937864.0
38	786980.0
39	315724.0
40	9576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.51508516151388	24.18102472400632	15.886004723284145	24.417885391195654
2	13.57715593548786	23.89182789146609	40.84193805628303	21.689078116763024
3	16.098898137904325	28.796956704573045	32.607405073827856	22.496740083694778
4	11.738339714958848	20.84755380593254	40.51264767827993	26.901458800828674
5	11.56044701603755	40.042691940958605	36.906338634610066	11.490522408393781
6	26.703752682178678	41.93665247386182	19.46861521469537	11.890979629264137
7	23.150352215900032	34.66071468369144	25.70151738278471	16.487415717623826
8	22.17578798816618	39.206087102708445	22.12324276461165	16.494882144513724
9	23.681755184820485	17.608691644914547	23.223653215576817	35.48589995468815
10-11	22.306007880244493	28.47675023108305	30.82260736153032	18.394634527142138
12-13	22.76067424200172	25.999599572441518	30.85358093621935	20.38614524933741
14-15	20.213720027974123	27.73833908841518	27.91862046043298	24.12932042317772
16-17	19.803528535989454	30.71682109781973	28.763073029492688	20.71657733669813
18-19	19.94830506584405	30.065860133189332	30.148862194595736	19.836972606370885
20-21	20.555602001311915	29.166254013985178	30.01733141262267	20.26081257208024
22-23	20.305310616731305	29.087434048747653	29.948369897286486	20.658885437234563
24-25	20.2246516184999	29.23050919278215	29.83711585961577	20.707723329102176
26-27	20.40128352675297	29.258045363344408	29.739979773676	20.60069133622662
28-29	20.343424490622933	29.205954379033265	29.826641444012193	20.62397968633161
30-31	20.69730058563531	29.187798940505523	29.650694701553437	20.46420577230573
32-33	20.567617637841202	29.291611845220917	29.572656362618506	20.56811415431937
34-35	20.562611796960763	29.239050094302634	29.644427086236863	20.553911022499737
36-37	20.606027120764235	29.18044646407518	29.624667849159497	20.58885856600109
38-39	20.545403785163234	29.228592843612045	29.621085785151397	20.60491758607332
40-41	20.68388184072952	29.19310554946995	29.529720765628802	20.59329184417173
42-43	20.625215671921087	29.24292132527761	29.383446007556014	20.748416995245293
44-45	20.706637630496502	29.345546634462057	29.17647692432323	20.771338810718213
46-47	20.701667177528027	29.251252680743328	29.070849375545166	20.97623076618348
48-49	20.714583986402076	29.30308908792228	29.117171333675916	20.86515559199973
50-51	20.626935784504433	29.399188233449657	29.057392708036957	20.916483274008954
52-53	20.731527176245567	29.36654698466833	28.955923766141638	20.946002072944463
54-55	20.757253096559232	29.27125216057633	29.043672639388753	20.927822103475684
56-57	20.81344802438001	29.302252136478312	28.962633017688177	20.921666821453503
58-59	20.717460484507548	29.29931893715868	29.0186654128634	20.964555165470376
60-61	20.79251465162606	29.29501398327819	28.972181335983986	20.940290029111768
62-63	20.81588112360721	29.325188847510137	28.935995032470764	20.92293499641189
64-65	20.668549420843586	29.312102496114427	29.033304298042513	20.986043784999474
66-67	20.753152647308855	29.265987504668118	28.902962088276112	21.077897759746918
68-69	20.62855817527965	29.29031094058493	29.016082828688045	21.06504805544737
70-71	20.709679993679096	29.07474336523117	29.05514909220248	21.16042754888725
72-73	20.807646415682253	29.12070232527209	28.99830298413486	21.0733482749108
74-75	20.607190613200242	29.249514921547625	29.021454428256376	21.121840036995753
76-77	20.700297581134137	29.209044044764838	29.026113283102184	21.06454509099884
78-79	20.65446776967396	29.194965615206225	28.954149171688194	21.196417443431624
80-81	20.78359675573012	29.210787168665885	28.895383198537957	21.11023287706604
82-83	20.8032318947009	29.169631497584085	29.002602132456463	21.024534475258548
84-85	20.810639216878723	29.16610256579527	29.041164623100276	20.982093594225734
86-87	20.673181519503533	29.220399348732705	28.995021505493273	21.111397626270488
88-89	20.76563137279673	29.17844846021368	28.910310150974727	21.145610016014864
90-91	20.69278239326903	29.207323486910575	29.03885124517631	21.06104287464408
92-93	20.67091286604656	29.127768252716095	29.091521077543405	21.10979780369394
94-95	20.505264371284383	29.172982612572447	29.171728181804312	21.150024834338858
96-97	20.45781062056375	29.36917795913599	29.253012201513872	20.919999218786383
98-99	21.47204600001316	31.37490519560035	29.273555424560154	17.879493379826332
100	20.739506456445284	32.13455807888802	23.158864252334684	23.967071212332012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1309.0
1	1184.0
2	1785.5
3	2629.0
4	3143.5
5	4744.5
6	6512.0
7	7872.0
8	8796.5
9	8880.0
10	8395.5
11	7702.0
12	7928.0
13	9685.5
14	14831.0
15	22703.0
16	29725.0
17	34461.0
18	37361.5
19	39093.5
20	40413.5
21	42549.5
22	46252.5
23	53900.0
24	67183.5
25	87353.0
26	115725.0
27	150541.0
28	192951.0
29	242249.5
30	291358.5
31	337622.0
32	377819.0
33	409461.5
34	436408.0
35	456794.5
36	470419.0
37	477041.5
38	470872.5
39	456172.5
40	440322.0
41	421195.0
42	400359.5
43	380992.5
44	362263.5
45	346438.0
46	330914.0
47	315190.0
48	300627.5
49	286008.5
50	272584.0
51	259029.5
52	243485.0
53	226403.5
54	208865.0
55	191498.0
56	173967.5
57	155655.5
58	136608.0
59	117266.5
60	98368.5
61	80734.5
62	64947.5
63	51051.5
64	38998.0
65	29598.0
66	22717.0
67	17708.0
68	13749.5
69	10404.0
70	7891.5
71	5905.5
72	4341.0
73	3136.5
74	2281.5
75	1682.5
76	1231.0
77	904.5
78	658.0
79	492.0
80	371.0
81	264.0
82	190.0
83	129.5
84	93.5
85	76.5
86	61.0
87	48.0
88	32.5
89	20.0
90	14.0
91	10.0
92	9.0
93	7.0
94	4.0
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02029377108733361
3	1.978717684392858
4	0.4910899737306027
5	4.2859073046111115E-5
6	1.6072152392291667E-4
7	1.0714768261527779E-5
8	0.0
9	0.02165454665654764
10-11	0.1531515401461473
12-13	0.004162687469603542
14-15	2.1429536523055558E-5
16-17	5.893122543840278E-5
18-19	0.07758563698172265
20-21	0.019096276740413445
22-23	6.806787514122074E-4
24-25	1.555110543692386E-4
26-27	0.0020932229170768134
28-29	0.001047738963198357
30-31	8.019314384141952E-4
32-33	0.0020831670442541853
34-35	0.02805023109079165
36-37	0.0
38-39	6.028652871757017E-5
40-41	0.0012924690699447807
42-43	7.795783873028955E-5
44-45	4.94715065765903E-4
46-47	0.0011764923862755286
48-49	0.003992944151695568
50-51	0.007507405942741776
52-53	0.004687018887630218
54-55	0.018201624913008682
56-57	0.0067129784189442045
58-59	0.007855907229087248
60-61	0.008797651611887997
62-63	0.007478179495517894
64-65	0.021590111387788602
66-67	0.0029662351467216502
68-69	0.0024844659094205353
70-71	0.001565667565967619
72-73	6.307820993434833E-4
74-75	1.090675488050014E-4
76-77	6.234382870908375E-5
78-79	7.042239635020438E-6
80-81	0.0
82-83	2.9234150047783218E-5
84-85	4.470582782936292E-5
86-87	3.0417406665138193E-5
88-89	3.111644965665721E-5
90-91	7.18805862197249E-5
92-93	0.0023660334213722323
94-95	1.0171049937142912E-4
96-97	1.7674484945624889E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	771.0
20-21	2342.0
22-23	4077.0
24-25	7836.0
26-27	9122.0
28-29	13011.0
30-31	24022.0
32-33	30382.0
34-35	42651.0
36-37	58228.0
38-39	70363.0
40-41	71707.0
42-43	81435.0
44-45	94810.0
46-47	96351.0
48-49	92101.0
50-51	88245.0
52-53	88729.0
54-55	96305.0
56-57	104283.0
58-59	107761.0
60-61	106272.0
62-63	102658.0
64-65	106597.0
66-67	115625.0
68-69	118485.0
70-71	119149.0
72-73	115807.0
74-75	117884.0
76-77	114753.0
78-79	127852.0
80-81	129735.0
82-83	130342.0
84-85	133913.0
86-87	141729.0
88-89	165469.0
90-91	171554.0
92-93	181524.0
94-95	215172.0
96-97	403555.0
98-99	694241.0
100-101	4636065.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.6817663668759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.72873202645123	84.99486979486169
2	4.453451013051498	8.076936085837254
3	1.018444920423716	2.770631529941848
4	0.3783770502090388	1.3724759706257492
5	0.1699925079962992	0.7707610447119843
6	0.0889179353670847	0.4837941264469767
7	0.05134146267915837	0.3259014166523582
8	0.030068704342076744	0.2181346577682296
9	0.020448790589455215	0.16688992056463336
>10	0.05982958987033059	0.7775572095703279
>50	2.368096907082142E-4	0.013933270763425858
>100	1.591893294757229E-4	0.02811497225549372
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0714768261527779E-5
2	0.0	0.0	0.0	0.0	1.0714768261527779E-5
3	0.0	0.0	0.0	0.0	1.0714768261527779E-5
4	0.0	2.1429536523055558E-5	0.0	2.1429536523055558E-5	2.1429536523055558E-5
5	0.0	2.1429536523055558E-5	0.0	2.1429536523055558E-5	2.1429536523055558E-5
6	7.500337783069445E-5	3.214430478458334E-5	0.0	3.214430478458334E-5	3.214430478458334E-5
7	7.500337783069445E-5	4.2859073046111115E-5	0.0	4.2859073046111115E-5	3.214430478458334E-5
8	7.500337783069445E-5	7.500337783069445E-5	0.0	4.2859073046111115E-5	3.214430478458334E-5
9	7.500337783069445E-5	1.0714768261527778E-4	0.0	5.357384130763889E-5	4.2859073046111115E-5
10-11	7.500337783069445E-5	1.0714768261527778E-4	0.0	5.893122543840278E-5	4.2859073046111115E-5
12-13	7.500337783069445E-5	1.0714768261527778E-4	0.0	1.1250506674604166E-4	4.2859073046111115E-5
14-15	7.500337783069445E-5	1.500067556613889E-4	0.0	1.3393460326909722E-4	8.571814609222223E-5
16-17	1.1786245087680555E-4	1.500067556613889E-4	0.0	1.392919873998611E-4	9.643291435375E-5
18-19	1.1786245087680555E-4	1.6072152392291667E-4	0.0	1.7679367631520833E-4	1.0714768261527778E-4
20-21	1.1786245087680555E-4	1.7143629218444446E-4	0.0	1.928658287075E-4	1.1250506674604166E-4
22-23	1.2857721913833335E-4	2.1429536523055555E-4	0.0	2.464396700151389E-4	1.1786245087680555E-4
24-25	1.2857721913833335E-4	2.3036751762284724E-4	0.0	2.6786920653819443E-4	1.3393460326909722E-4
26-27	1.2857721913833335E-4	2.464396700151389E-4	0.0	3.0001351132277775E-4	1.392919873998611E-4
28-29	1.2857721913833335E-4	2.517970541459028E-4	0.0	5.785974861225E-4	1.392919873998611E-4
30-31	1.2857721913833335E-4	2.946561271920139E-4	0.0	0.0015054249407446527	1.392919873998611E-4
32-33	1.2857721913833335E-4	3.000135113227778E-4	0.0	0.004103756244165139	1.392919873998611E-4
34-35	1.392919873998611E-4	3.3215781610736113E-4	0.0	0.007918213745269027	1.392919873998611E-4
36-37	1.392919873998611E-4	3.85731657415E-4	0.0	0.013972057813032224	1.392919873998611E-4
38-39	1.44649371530625E-4	4.0716119393805557E-4	0.0	0.024236805807575833	1.392919873998611E-4
40-41	1.6072152392291667E-4	4.285907304611111E-4	0.0	0.035048006983457364	1.500067556613889E-4
42-43	1.6072152392291667E-4	4.5537765111493055E-4	0.0	0.04481451825383993	1.553641397921528E-4
44-45	1.6072152392291667E-4	5.30381028945625E-4	0.0	0.05693292115762785	1.7679367631520833E-4
46-47	1.6072152392291667E-4	5.678827178609722E-4	0.0	0.06947991479187687	1.8215106044597223E-4
48-49	1.6072152392291667E-4	5.678827178609722E-4	0.0	0.08107865143498069	2.0358059696902779E-4
50-51	1.8215106044597223E-4	6.107417909070834E-4	0.0	0.09231844334132333	2.0358059696902779E-4
52-53	1.928658287075E-4	6.214565591686111E-4	0.0	0.10498329942644917	2.4108228588437498E-4
54-55	1.982232128382639E-4	6.26813943299375E-4	0.0	0.11755172259722126	2.785839747997222E-4
56-57	2.0358059696902779E-4	6.428860956916667E-4	0.0	0.1285075731446334	2.8929874306125E-4
58-59	2.0358059696902779E-4	6.428860956916667E-4	0.0	0.1385526683898157	2.8929874306125E-4
60-61	2.0893798109979165E-4	6.428860956916667E-4	0.0	0.1481638155204061	2.8929874306125E-4
62-63	2.357249017536111E-4	6.589582480839584E-4	0.0	0.15946789603631792	3.1072827958430555E-4
64-65	2.357249017536111E-4	7.7146331483E-4	0.0	0.1706862584061375	3.1072827958430555E-4
66-67	2.357249017536111E-4	7.7146331483E-4	0.0	0.1801259692445435	3.1072827958430555E-4
68-69	2.357249017536111E-4	7.821780830915277E-4	0.0	0.18988176574666452	3.1072827958430555E-4
70-71	2.357249017536111E-4	7.928928513530556E-4	0.0	0.19808392085086401	3.268004319765972E-4
72-73	2.357249017536111E-4	7.982502354838194E-4	0.0	0.2052253138971723	3.5358735263041666E-4
74-75	2.357249017536111E-4	8.196797720068751E-4	0.0	0.21074877693598987	3.5358735263041666E-4
76-77	2.41082285884375E-4	8.357519243991667E-4	0.0	0.21559720957433118	3.5358735263041666E-4
78-79	2.464396700151389E-4	8.357519243991667E-4	0.0	0.21837769193819764	3.5358735263041666E-4
80-81	2.464396700151389E-4	8.357519243991667E-4	0.0	0.21947595568500425	3.6430212089194445E-4
82-83	2.517970541459028E-4	8.411093085299306E-4	0.0	0.22041349790788795	4.0716119393805557E-4
84-85	2.6251182240743056E-4	8.464666926606945E-4	0.0	0.2208474460224798	4.0716119393805557E-4
86-87	2.732265906689583E-4	8.571814609222222E-4	0.0	0.22104566923531807	4.2323334633034723E-4
88	2.8929874306125E-4	9.321848387529167E-4	0.0	0.2210563840035796	4.393054987226389E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8360	0.0	42.063744	1
GTATCAA	14920	0.0	31.239027	1
ATCAACG	17170	0.0	25.97784	3
TCAACGC	17305	0.0	25.810656	4
CAACGCA	17720	0.0	25.393047	5
AACGCAG	18245	0.0	24.440596	6
TATCAAC	19300	0.0	23.34889	2
ACGCAGA	21075	0.0	21.04218	7
CGCAGAG	21470	0.0	20.388535	8
GTACATG	20060	0.0	18.258738	1
TACATGG	20575	0.0	17.050673	2
AGAGTAC	22930	0.0	16.63569	10-11
ACATGGG	20320	0.0	16.606947	3
GCAGAGT	25445	0.0	16.432428	9
GAGTACT	14895	0.0	15.146207	12-13
CAGAGTA	25420	0.0	14.652497	10-11
CATGGGG	13200	0.0	13.86371	4
TATACTG	6520	0.0	13.288609	5
AGTACTT	17970	0.0	13.167373	12-13
TATACAG	8580	0.0	13.146587	5
>>END_MODULE