FastQCFastQC Report
Fri 27 May 2016
SRR2140181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11166958
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101750.043.5668561
GTATCAA179400.031.6266061
TCAACGC214450.025.3771174
ATCAACG215650.025.1689343
CAACGCA221000.024.7144245
TATCAAC233250.023.8413942
AACGCAG225850.023.7690776
ACGCAGA263300.020.4038667
CGCAGAG271200.019.7491578
GTACATG240600.019.5358431
TACATGG248700.018.1603892
ACATGGG240750.017.9630133
AGAGTAC289900.016.56597510-11
GCAGAGT311700.016.4217369
GTGGTAT56100.015.7142931
CATGGGG167600.015.5997234
GAGTACT192100.015.45539612-13
CAGAGTA321800.014.9654239
AGTACTT230350.013.67794412-13
TATACTG70700.013.5990935
Produced by FastQC (version 0.11.5)