##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11166958
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.789073085078318	31.0	30.0	31.0	26.0	34.0
2	30.282592179535374	31.0	30.0	31.0	27.0	34.0
3	29.85291589705988	31.0	30.0	31.0	26.0	34.0
4	33.73586978656139	35.0	33.0	37.0	28.0	37.0
5	33.49189286822786	35.0	33.0	37.0	28.0	37.0
6	33.535959569293624	35.0	33.0	37.0	28.0	37.0
7	33.49742982824866	35.0	33.0	37.0	28.0	37.0
8	33.421776369177714	35.0	33.0	37.0	28.0	37.0
9	34.646504177771604	37.0	33.0	39.0	28.0	39.0
10-11	34.65279993889115	37.0	33.0	39.0	27.5	39.0
12-13	34.50693689364641	37.0	33.0	39.0	27.0	39.0
14-15	35.339678361824234	37.0	33.0	40.0	27.0	41.0
16-17	35.254410601347296	37.0	33.0	40.0	27.0	41.0
18-19	35.41335849924393	37.5	33.5	40.0	27.0	41.0
20-21	35.43219815134443	38.0	33.0	40.0	27.0	41.0
22-23	35.38434336169275	37.5	33.0	40.0	27.0	41.0
24-25	35.387803027456414	37.0	33.0	40.0	27.0	41.0
26-27	35.0745377242409	37.0	33.0	40.0	27.0	41.0
28-29	34.830834930338796	37.0	33.0	40.0	26.0	41.0
30-31	34.773285847174236	37.0	33.0	40.0	25.0	41.0
32-33	34.73031143911358	37.0	33.0	40.0	25.5	41.0
34-35	34.509439303732194	37.0	32.5	40.0	25.0	41.0
36-37	34.408213761858974	37.0	32.0	40.0	25.0	41.0
38-39	34.37839343248351	37.0	32.0	40.0	25.0	41.0
40-41	34.295615386997305	37.0	32.0	40.0	24.5	41.0
42-43	34.30188699746591	37.0	32.0	40.0	24.5	41.0
44-45	34.366099611729396	37.0	32.0	40.0	24.5	41.0
46-47	34.20540749509081	37.0	32.0	40.0	24.0	41.0
48-49	34.18905303380225	37.0	32.0	40.0	24.0	41.0
50-51	34.13920151095898	37.0	32.0	39.5	24.0	41.0
52-53	34.18399227090305	37.0	32.0	39.0	24.0	41.0
54-55	34.29682023235985	37.0	32.0	39.0	24.5	41.0
56-57	34.27055589538778	36.5	32.0	39.0	25.0	40.5
58-59	34.00542363323222	36.0	31.0	39.0	24.0	40.0
60-61	33.688553651194184	36.0	31.0	39.0	23.5	40.0
62-63	33.59714541079147	36.0	31.0	39.0	23.5	40.0
64-65	33.4095496819597	35.5	31.0	39.0	23.5	40.0
66-67	33.17093232355842	35.0	31.0	38.5	23.0	40.0
68-69	33.00872306640033	35.0	30.5	38.0	23.0	40.0
70-71	32.99587811923786	35.0	31.0	38.0	24.0	40.0
72-73	32.80242794430822	34.5	30.0	37.5	24.0	40.0
74-75	32.44740787144491	34.0	30.0	37.0	23.0	39.0
76-77	31.497039254381214	33.5	28.5	36.0	22.5	39.0
78-79	31.947697800200825	34.0	30.0	36.0	24.0	39.0
80-81	31.960925406156086	34.0	30.0	36.0	24.5	38.5
82-83	31.970368419084004	34.0	30.0	35.5	25.0	37.0
84-85	31.698750408701457	34.0	30.0	35.0	25.0	37.0
86-87	31.50098730800333	34.0	30.0	35.0	25.0	36.5
88-89	31.455945118243847	34.0	30.0	35.0	25.0	36.0
90-91	31.33604350686047	34.0	30.0	35.0	25.0	36.0
92-93	31.26642746387834	33.0	30.0	35.0	25.0	35.5
94-95	31.452140626944118	33.5	30.0	35.0	25.0	35.0
96-97	31.690389566439855	34.0	30.5	35.0	25.5	35.0
98-99	32.13410709282537	34.0	31.0	35.0	26.5	35.0
100	31.71263186561174	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	27.0
19	188.0
20	1226.0
21	6313.0
22	21535.0
23	56892.0
24	117119.0
25	202204.0
26	304355.0
27	411527.0
28	516357.0
29	615683.0
30	704195.0
31	786923.0
32	867494.0
33	943410.0
34	1016694.0
35	1076652.0
36	1119044.0
37	1113777.0
38	914470.0
39	360310.0
40	10561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.78921851411996	23.968120951113097	15.717942164732776	24.52471837003417
2	13.758370386267094	23.902156250554203	40.544322987770876	21.795150375407825
3	16.366773831246007	28.65873732772915	32.37773533069558	22.59675351032927
4	11.917907225810469	20.63345947236846	40.16154218962896	27.287091112192112
5	11.700081920265468	39.84543325862482	36.69739542270965	11.757089398400066
6	26.98394887912953	41.660459359255434	19.29738891904734	12.058202842567699
7	23.35516082356538	34.49623433705043	25.524516166354346	16.624088673029842
8	22.309907496741726	39.10372009995918	22.009100419290554	16.577271984008537
9	23.761222932400866	17.55241548316829	23.078388555296005	35.60797302913484
10-11	22.542077337227017	28.34624816183081	30.596668574228225	18.515005926713943
12-13	22.956502520110533	25.85105083972564	30.675845809704754	20.516600830459065
14-15	20.442381015598922	27.578966786611304	27.793107478021657	24.18554471976812
16-17	20.009233493339657	30.605802145965583	28.521308114938588	20.863656245756175
18-19	20.128072122086262	29.849158514870254	30.05889295863217	19.96387640441131
20-21	20.775043226145154	28.954935296385358	29.889680669610087	20.380340807859398
22-23	20.492623579227164	28.82278303979154	29.873812015166855	20.81078136581444
24-25	20.408400276268612	29.01536254473352	29.76895605147984	20.807281127518028
26-27	20.60228170211521	28.998625479572304	29.61281185492236	20.786280963390126
28-29	20.584514749744084	28.901953413689835	29.73486253978459	20.778669296781494
30-31	20.87898874533681	28.91386425103164	29.498850233328383	20.70829677030317
32-33	20.77549731447611	29.056929893525798	29.43836625417725	20.729206537820847
34-35	20.748278980090713	28.96022582100668	29.526259710466373	20.765235488436232
36-37	20.813897518726137	28.876582188455924	29.499373349024456	20.81014694379349
38-39	20.724316176248887	29.031140130725735	29.490437973643886	20.754105719381496
40-41	20.88419051812684	28.932893543320876	29.376702383905428	20.806213554646856
42-43	20.75073627884597	29.093321202298295	29.199340514628002	20.956602004227737
44-45	20.872040614328373	29.110123408979184	29.014246222015842	21.003589754676597
46-47	20.89579429127498	28.984242846988074	28.943826001345645	21.1761368603913
48-49	20.86375114833826	29.119331786492996	28.944622981443967	21.072294083724774
50-51	20.78575662194092	29.125837251667658	28.962077079696662	21.126329046694753
52-53	20.96484878105609	29.113336297430042	28.774727146529997	21.147087774983873
54-55	20.89636037862664	28.998029435853283	28.97117838070792	21.134431804812156
56-57	20.971939216920994	29.115728010527793	28.80062839419381	21.111704378357405
58-59	20.906180457358587	29.094487187539013	28.859440643550787	21.139891711551613
60-61	20.941722617182844	29.07226265440763	28.84600883803327	21.14000589037625
62-63	20.985117682348182	29.128517882043354	28.79928916230403	21.087075273304436
64-65	20.886229423224677	29.071112092206707	28.86973829216294	21.172920192405677
66-67	20.93775174947497	29.117976824872766	28.715589557819598	21.228681867832663
68-69	20.854603830209534	29.095965880342327	28.78248392497671	21.26694636447143
70-71	20.897470586393332	28.88117485069924	28.848466726029738	21.372887836877695
72-73	20.961764526060318	29.008611934204946	28.736126878107783	21.29349666162695
74-75	20.760056051690192	29.06396929848503	28.81400659956112	21.361968050263656
76-77	20.88010387711295	29.01094554932372	28.879308253114267	21.229642320449063
78-79	20.800768101927577	29.078138529498403	28.74987383355118	21.37121953502284
80-81	20.96703513502203	29.072404330748675	28.68718052956615	21.27338000466315
82-83	20.96120963073337	29.001598463233975	28.834199865459887	21.202992040572767
84-85	20.963736979138375	29.008026671849795	28.846331127667966	21.18190522134386
86-87	20.77962287264517	29.089732671501928	28.878565675830348	21.252078780022558
88-89	20.891949367587124	29.035461460568108	28.77966826946028	21.292920902384484
90-91	20.85619979373646	29.046552372482804	28.915769427360715	21.18147840642002
92-93	20.84049383094188	28.968490437659096	28.962713269792236	21.228302461606788
94-95	20.631185734166227	29.0610421217412	29.020891182986443	21.28688096110613
96-97	20.649900918398497	29.215999508395896	29.06045473526553	21.073644837940076
98-99	21.635444182567525	31.21498698844168	29.12255782572134	18.027011003269454
100	20.916412252035315	32.01759760921913	23.010280501089326	24.055709637656232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1564.0
1	1427.5
2	2267.0
3	3388.5
4	4040.0
5	6157.0
6	8580.5
7	10504.5
8	11872.5
9	12011.5
10	11179.5
11	10086.5
12	10416.5
13	12878.5
14	19434.5
15	29549.0
16	38509.0
17	44172.0
18	47626.5
19	49985.5
20	51689.5
21	53484.0
22	57095.5
23	65327.0
24	79876.5
25	103260.0
26	136026.5
27	175743.5
28	224826.0
29	281636.0
30	335810.0
31	385707.5
32	432274.0
33	470805.5
34	502171.0
35	526198.0
36	544720.5
37	554199.5
38	547568.5
39	529473.0
40	509323.0
41	488172.5
42	464577.5
43	442611.0
44	422013.5
45	405132.5
46	389228.0
47	373977.0
48	360881.0
49	345377.0
50	330223.0
51	315875.5
52	299177.5
53	280276.0
54	262315.0
55	244992.0
56	226794.0
57	207031.5
58	185273.0
59	161443.0
60	136006.0
61	112398.0
62	91631.0
63	73593.5
64	57673.5
65	44746.5
66	34878.5
67	26729.0
68	20293.0
69	15468.0
70	11632.5
71	8509.5
72	6290.0
73	4572.0
74	3355.0
75	2457.0
76	1742.0
77	1241.0
78	892.5
79	660.5
80	500.0
81	377.5
82	294.5
83	208.5
84	146.5
85	106.0
86	80.0
87	64.5
88	45.0
89	29.5
90	21.5
91	15.5
92	9.5
93	6.0
94	3.5
95	2.5
96	3.0
97	3.0
98	1.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02059647757249557
3	1.9137888760752928
4	0.48199339515739203
5	1.7909980497822234E-5
6	1.0745988298693342E-4
7	0.0
8	0.0
9	0.020757667396975973
10-11	0.14862597316117782
12-13	0.004329737785348526
14-15	4.4774951244555585E-6
16-17	3.1342465871188915E-5
18-19	0.07453686133681169
20-21	0.018951726790258614
22-23	7.705037544587305E-4
24-25	1.1205638892639043E-4
26-27	0.0026696409907176807
28-29	0.0012308297148886282
30-31	8.100051840331778E-4
32-33	0.0020261393638220226
34-35	0.027300541644920136
36-37	4.551668400397161E-6
38-39	5.040529292234079E-5
40-41	0.0011359101748091877
42-43	1.0707129062569902E-4
44-45	5.686800808428084E-4
46-47	0.0014444561014585965
48-49	0.0036839610948732372
50-51	0.007289994880016246
52-53	0.0047508931213294265
54-55	0.0186505208519661
56-57	0.006713291032747824
58-59	0.007843577638015999
60-61	0.008696961201524021
62-63	0.0074600091155893515
64-65	0.020408157881477037
66-67	0.00314526502816189
68-69	0.0026166695312502546
70-71	0.0018026907060709496
72-73	5.990738542166653E-4
74-75	9.097815159689401E-5
76-77	4.6216882426330865E-5
78-79	0.0
80-81	0.0
82-83	2.436216947311753E-5
84-85	6.207833677733598E-5
86-87	6.334119080805308E-5
88-89	2.590966065664019E-5
90-91	4.653958567402517E-5
92-93	0.0022356114304966524
94-95	7.052680561669837E-5
96-97	1.2495902079171094E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1089.0
20-21	3114.0
22-23	5548.0
24-25	10396.0
26-27	12316.0
28-29	16628.0
30-31	29150.0
32-33	36899.0
34-35	51069.0
36-37	69072.0
38-39	82514.0
40-41	85629.0
42-43	97245.0
44-45	113497.0
46-47	115586.0
48-49	108950.0
50-51	104398.0
52-53	104682.0
54-55	115807.0
56-57	123546.0
58-59	126206.0
60-61	125874.0
62-63	120512.0
64-65	126212.0
66-67	136271.0
68-69	139541.0
70-71	141072.0
72-73	136563.0
74-75	139918.0
76-77	135513.0
78-79	150828.0
80-81	153404.0
82-83	154709.0
84-85	159177.0
86-87	167698.0
88-89	196246.0
90-91	203618.0
92-93	216908.0
94-95	256476.0
96-97	484721.0
98-99	826916.0
100-101	5581440.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.32960914316936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.63252478233134	84.5778936667611
2	4.47284294494571	8.080603099514573
3	1.045063895775223	2.8320063970504137
4	0.3978790925433325	1.4376105166271254
5	0.1856163286179074	0.8383325207322831
6	0.08598667697199334	0.46602857534400727
7	0.054353856751818835	0.3436833845070929
8	0.030847107054799067	0.2229125698766002
9	0.02423821045961508	0.19704852694322192
>10	0.0696057932677603	0.9293014829661276
>50	9.157418092404394E-4	0.052220320630864646
>100	1.2556947130812037E-4	0.022358939046619085
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.954990248911119E-6	0.0	0.0	0.0
3	0.0	8.954990248911119E-6	0.0	0.0	0.0
4	0.0	3.5819960995644474E-5	0.0	8.954990248911119E-6	0.0
5	0.0	3.5819960995644474E-5	0.0	8.954990248911119E-6	0.0
6	7.163992199128895E-5	3.5819960995644474E-5	0.0	1.7909980497822237E-5	8.954990248911119E-6
7	7.163992199128895E-5	3.5819960995644474E-5	0.0	1.7909980497822237E-5	8.954990248911119E-6
8	7.163992199128895E-5	3.5819960995644474E-5	0.0	1.7909980497822237E-5	8.954990248911119E-6
9	7.163992199128895E-5	8.059491224020007E-5	0.0	4.477495124455559E-5	8.954990248911119E-6
10-11	8.059491224020007E-5	8.059491224020007E-5	0.0	7.163992199128895E-5	1.7909980497822237E-5
12-13	8.954990248911119E-5	8.059491224020007E-5	0.0	8.954990248911119E-5	1.7909980497822237E-5
14-15	9.850489273802229E-5	1.1193737811138898E-4	0.0	1.0745988298693341E-4	1.7909980497822237E-5
16-17	1.7014481472931125E-4	1.2536986348475566E-4	0.0	1.1641487323584453E-4	1.7909980497822237E-5
18-19	1.7014481472931125E-4	1.4775733910703346E-4	0.0	1.2536986348475566E-4	1.7909980497822237E-5
20-21	1.7014481472931125E-4	1.746223098537668E-4	0.0	1.2984735860921122E-4	1.7909980497822237E-5
22-23	1.7014481472931125E-4	2.1044227084941126E-4	0.0	1.7014481472931125E-4	2.6864970746733352E-5
24-25	1.7014481472931125E-4	2.283522513472335E-4	0.0	2.2387475622277794E-4	2.6864970746733352E-5
26-27	1.7014481472931125E-4	2.4178473672060018E-4	0.0	2.865596879651558E-4	2.6864970746733352E-5
28-29	1.7014481472931125E-4	2.4626223184505576E-4	0.0	6.716242686683338E-4	2.6864970746733352E-5
30-31	1.746223098537668E-4	3.402896294586225E-4	0.0	0.00400288064126327	2.6864970746733352E-5
32-33	1.8357730010267793E-4	3.402896294586225E-4	0.0	0.011493729984477419	2.6864970746733352E-5
34-35	1.8805479522713346E-4	4.4774951244555593E-4	0.0	0.019584563674368616	2.6864970746733352E-5
36-37	1.8805479522713346E-4	5.641643856814005E-4	0.0	0.031020086222228113	2.6864970746733352E-5
38-39	1.8805479522713346E-4	5.820743661792227E-4	0.0	0.04836590233436895	2.6864970746733352E-5
40-41	1.9253229035158902E-4	6.671467735438783E-4	0.0	0.06502218419734362	2.6864970746733352E-5
42-43	2.0148728060050014E-4	7.208767150373449E-4	0.0	0.07926509618823677	2.6864970746733352E-5
44-45	2.059647757249557E-4	8.372915882731895E-4	0.0	0.09560347589737508	2.6864970746733352E-5
46-47	2.059647757249557E-4	9.13409005388934E-4	0.0	0.11184335071377541	3.1342465871188915E-5
48-49	2.059647757249557E-4	9.268414907623007E-4	0.0	0.12503405135042148	3.5819960995644474E-5
50-51	2.1491976597386682E-4	9.984814127535898E-4	0.0	0.13695762086684665	4.477495124455559E-5
52-53	2.2387475622277794E-4	0.0010119138981269564	0.0	0.14986623931065202	4.925244636901115E-5
54-55	2.2387475622277794E-4	0.0010343013737492343	0.0	0.16252411802748787	5.3729941493466705E-5
56-57	2.2387475622277794E-4	0.001056688849371512	0.0	0.17291638421134925	6.268493174237783E-5
58-59	2.2387475622277794E-4	0.001056688849371512	0.0	0.18220270909947006	6.268493174237783E-5
60-61	2.3282974647168906E-4	0.0010925088103671565	0.0	0.19088904964091385	7.163992199128895E-5
62-63	2.3282974647168906E-4	0.0011193737811138898	0.0	0.20056043910973786	7.163992199128895E-5
64-65	2.3282974647168906E-4	0.00119996869335409	0.0	0.20940796947566204	7.163992199128895E-5
66-67	2.3282974647168906E-4	0.001208923683603001	0.0	0.2173734333020685	7.163992199128895E-5
68-69	2.3282974647168906E-4	0.0012357886543497344	0.0	0.22592992648490307	7.163992199128895E-5
70-71	2.3730724159614462E-4	0.0012536986348475566	0.0	0.23282079148144016	8.059491224020007E-5
72-73	2.552172220939669E-4	0.0012536986348475566	0.0	0.23887884238482854	8.059491224020007E-5
74-75	2.5969471721842244E-4	0.0013074285763410233	0.0	0.2440906467096948	8.507240736465563E-5
76-77	2.5969471721842244E-4	0.0013835459934567677	0.0	0.2488367915416177	8.954990248911119E-5
78-79	2.5969471721842244E-4	0.0014148884593279567	0.0	0.2514785136650465	8.954990248911119E-5
80-81	2.5969471721842244E-4	0.0014193659544524122	0.0	0.25217700290446154	9.85048927380223E-5
82-83	2.64172212342878E-4	0.0014238434495768679	0.0	0.2532023492879618	1.0745988298693341E-4
84-85	2.6864970746733356E-4	0.0014238434495768679	0.0	0.2536545762955319	1.0745988298693341E-4
86-87	2.6864970746733356E-4	0.0014238434495768679	0.0	0.25378890114926556	1.0745988298693341E-4
88	2.6864970746733356E-4	0.0014686184008214232	0.0	0.25379337864439	1.0745988298693341E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10175	0.0	43.566856	1
GTATCAA	17940	0.0	31.626606	1
TCAACGC	21445	0.0	25.377117	4
ATCAACG	21565	0.0	25.168934	3
CAACGCA	22100	0.0	24.714424	5
TATCAAC	23325	0.0	23.841394	2
AACGCAG	22585	0.0	23.769077	6
ACGCAGA	26330	0.0	20.403866	7
CGCAGAG	27120	0.0	19.749157	8
GTACATG	24060	0.0	19.535843	1
TACATGG	24870	0.0	18.160389	2
ACATGGG	24075	0.0	17.963013	3
AGAGTAC	28990	0.0	16.565975	10-11
GCAGAGT	31170	0.0	16.421736	9
GTGGTAT	5610	0.0	15.714293	1
CATGGGG	16760	0.0	15.599723	4
GAGTACT	19210	0.0	15.455396	12-13
CAGAGTA	32180	0.0	14.965423	9
AGTACTT	23035	0.0	13.677944	12-13
TATACTG	7070	0.0	13.599093	5
>>END_MODULE