FastQCFastQC Report
Fri 27 May 2016
SRR2140180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8668631
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC141250.056.7994231
GGTATCA92250.046.5025141
TATACAC191300.044.386483
TTATACA188200.043.324552
GTATCAA165750.034.890391
TACACAT274700.030.9871675
ACACATC298900.027.6712866
ATACACA317600.027.6117124
TCAACGC221500.027.2702184
ATCAACG223200.027.0437743
ACATCTC308900.027.0074848
TATCAAC227150.026.4249422
CAACGCA229050.026.389755
CACATCT324700.025.511537
AACGCAG237800.025.4742366
CATCTCC343300.024.0924769
ACGCAGA284850.021.207347
CGCAGAG301650.020.0681518
ATCTCCG279700.019.1411277
TCTCCGA289250.018.4945818
Produced by FastQC (version 0.11.5)