##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8668631
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.041127139914018	31.0	18.0	33.0	18.0	33.0
2	26.830611084956782	29.0	18.0	32.0	18.0	33.0
3	29.721480589034186	32.0	27.0	33.0	25.0	33.0
4	30.739948095610483	32.0	31.0	33.0	27.0	33.0
5	31.503294464835335	33.0	32.0	33.0	28.0	33.0
6	35.237777683696535	37.0	35.0	38.0	29.0	38.0
7	35.753950075853965	38.0	36.0	38.0	31.0	38.0
8	36.198097369700015	38.0	37.0	38.0	33.0	38.0
9	36.343430006422004	38.0	37.0	38.0	33.0	38.0
10-11	36.49570122433404	38.0	38.0	38.0	34.0	38.0
12-13	36.514453377932455	38.0	38.0	38.0	34.0	38.0
14-15	36.52618095060224	38.0	38.0	38.0	34.0	38.0
16-17	36.51821527528395	38.0	38.0	38.0	34.0	38.0
18-19	36.53830887483848	38.0	38.0	38.0	34.0	38.0
20-21	36.532797829948414	38.0	38.0	38.0	34.0	38.0
22-23	36.523931675390784	38.0	38.0	38.0	34.0	38.0
24-25	36.50751060570059	38.0	38.0	38.0	34.0	38.0
26-27	36.44944870082084	38.0	38.0	38.0	34.0	38.0
28-29	36.42261193565781	38.0	38.0	38.0	34.0	38.0
30-31	36.417292460220466	38.0	38.0	38.0	34.0	38.0
32-33	36.369033107417714	38.0	38.0	38.0	33.5	38.0
34-35	36.29962756046995	38.0	37.5	38.0	33.0	38.0
36-37	36.28399651070977	38.0	37.0	38.0	33.0	38.0
38-39	36.220142322021395	38.0	37.0	38.0	33.0	38.0
40-41	36.32251905837039	38.0	37.0	38.0	33.0	38.0
42-43	36.29586838703959	38.0	37.0	38.0	33.0	38.0
44-45	36.24381887186648	38.0	37.0	38.0	33.0	38.0
46-47	36.20132201004128	38.0	37.0	38.0	33.0	38.0
48-49	36.15489861759017	38.0	37.0	38.0	33.0	38.0
50-51	36.12643745616717	38.0	37.0	38.0	33.0	38.0
52-53	36.10134195100214	38.0	37.0	38.0	33.0	38.0
54-55	36.057006995534536	38.0	37.0	38.0	32.5	38.0
56-57	36.05325298250176	38.0	37.0	38.0	32.0	38.0
58-59	36.01380368629671	38.0	37.0	38.0	31.0	38.0
60-61	35.998319929354864	38.0	37.0	38.0	31.0	38.0
62-63	35.99126976245983	38.0	37.0	38.0	31.0	38.0
64-65	35.97568375402021	38.0	37.0	38.0	31.0	38.0
66-67	35.96228176726906	38.0	37.0	38.0	31.0	38.0
68-69	35.93126211483212	38.0	37.0	38.0	31.0	38.0
70-71	35.90061783147429	38.0	37.0	38.0	31.0	38.0
72-73	35.860115111882806	38.0	37.0	38.0	31.0	38.0
74-75	35.8841290277079	38.0	37.0	38.0	31.0	38.0
76-77	35.86528162188668	38.0	37.0	38.0	31.0	38.0
78-79	35.85659672104565	38.0	37.0	38.0	31.0	38.0
80-81	35.81827630328952	38.0	37.0	38.0	31.0	38.0
82-83	35.822729025780916	38.0	37.0	38.0	31.0	38.0
84-85	35.82384192928521	38.0	37.0	38.0	31.0	38.0
86-87	35.82356712719238	38.0	37.0	38.0	31.0	38.0
88-89	35.800537708793996	38.0	37.0	38.0	31.0	38.0
90-91	35.78828675126139	38.0	37.0	38.0	31.0	38.0
92-93	35.741495976831416	38.0	37.0	38.0	31.0	38.0
94-95	35.73889112315908	38.0	36.5	38.0	31.0	38.0
96-97	35.779671382660766	38.0	37.0	38.0	31.0	38.0
98-99	35.820915244985784	38.0	37.0	38.0	31.0	38.0
100-101	35.317628540727895	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	30.0
18	263.0
19	606.0
20	854.0
21	1475.0
22	3461.0
23	8581.0
24	18589.0
25	33895.0
26	55412.0
27	82674.0
28	114274.0
29	152971.0
30	194431.0
31	242920.0
32	304876.0
33	392397.0
34	536920.0
35	830444.0
36	1777092.0
37	3916466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.498119599276976	24.603054392325614	13.720447900020199	26.178378108377203
2	12.869875300955828	24.428989998536103	39.068118137685175	23.633016562822895
3	18.919549352707875	26.40005906497378	30.545670375916256	24.13472120640209
4	13.309148814847466	18.972199877927668	37.20327927212498	30.51537203509989
5	13.54284895167171	38.862393393815545	34.157675241327524	13.437082413185223
6	29.353181603877243	38.461782489068916	18.19197287322531	13.993063033828527
7	25.501593042776882	32.787276330022586	22.560055907328387	19.151074719872145
8	23.648024699632504	37.4810509294951	21.34375081832414	17.527173552548263
9	24.43263532615473	16.723344205099973	20.82485688916739	38.019163579577906
10-11	23.878557063970078	26.998986345133392	28.78972469816745	20.332731892729083
12-13	24.85855032934266	24.107566696517594	28.707220321178745	22.326662652961005
14-15	21.90505052066468	25.61023764882829	25.85550705757345	26.62920477293358
16-17	21.838119536983406	29.057858155457307	26.464767043377435	22.639255264181855
18-19	22.012155091155687	27.753038513232365	28.206841426287493	22.027964969324454
20-21	22.62030845243282	26.841913181945266	28.112117976857615	22.4256603887643
22-23	22.117560147811023	27.600496041583778	27.971056203182066	22.310887607423133
24-25	22.448697006339994	27.77130092473384	28.14118061373489	21.638821455191277
26-27	22.502755924118787	27.795405548595813	28.035038077427632	21.666800449857764
28-29	22.104396495828794	27.61016355146932	28.12671298325109	22.1587269694508
30-31	23.154634682182724	27.363008239629	28.093925445381174	21.388431632807105
32-33	21.566672315732003	28.11024965602642	28.343063428026394	21.980014600215185
34-35	22.140502748926348	27.771522298334006	28.655501838339042	21.432473114400604
36-37	21.87074655091057	27.684755866532424	28.462166843186175	21.982330739370834
38-39	21.768841598569352	26.97072438892073	28.919663331356137	22.340770681153778
40-41	22.009932898703465	26.79866601214343	29.104465340139065	22.086935749014046
42-43	22.388420609870995	26.641129395785363	28.80872047289198	22.16172952145166
44-45	21.744775005975214	27.20294125457798	28.701633949846816	22.350649789599984
46-47	22.493163659900254	26.52746230922513	28.220498492771327	22.758875538103283
48-49	22.233426600343915	26.699730797445557	28.190872544570688	22.87597005763984
50-51	21.832436669723478	26.77439008751436	28.738117859063767	22.655055383698393
52-53	22.041792527341627	26.855087522138977	29.19224214439666	21.910877806122738
54-55	22.01242053435914	26.937265132463413	28.504893376870427	22.545420956307023
56-57	22.54189958371188	27.696373438601007	27.554176706469097	22.207550271218015
58-59	21.61997379053292	28.55163428389713	28.419544950445275	21.408846975124675
60-61	22.22916670320802	29.302500617305245	27.060310251346685	21.40802242814005
62-63	21.598168763864425	29.89712551728917	27.126405767312427	21.37829995153398
64-65	21.604704075725024	29.651032078146695	27.210025907682727	21.534237938445553
66-67	21.791510429809335	29.372578537647918	27.10466855888574	21.731242473657
68-69	21.930345293048322	28.70793449826836	27.421255219956613	21.9404649887267
70-71	22.097853806392084	27.905933946327266	27.72097463890671	22.275237608373942
72-73	22.244111086899224	27.554913371197642	27.826438329180046	22.37453721272308
74-75	22.12684713250547	27.61811544751692	27.837532637602187	22.41750478237542
76-77	22.241172801447977	27.672215552022152	27.719382880300557	22.367228766229317
78-79	22.166162621119465	27.656306668673857	27.7547440849523	22.422786625254375
80-81	22.233911835213277	27.634839956236096	27.758599749707745	22.372648458842882
82-83	22.37365759068904	27.515394621008948	27.900698372840367	22.21024941546165
84-85	22.39426555194515	27.537824724358316	27.76725996388254	22.30064975981399
86-87	22.179695851354875	27.707235837932824	27.784521685881085	22.328546624831212
88-89	22.39028745561561	27.548351495416508	27.666164400680586	22.395196648287296
90-91	22.36494441056346	27.587266595372007	27.755757090758916	22.29203190330562
92-93	22.459031837210333	27.553365485094716	27.75197597167774	22.235626706017214
94-95	22.13954478995453	27.74918789817728	27.77675714023723	22.33451017163096
96-97	22.20979792571569	27.695072394898425	27.83893457362928	22.2561951057566
98-99	22.502986082445737	28.286938821965556	27.808021586101678	21.402053509487033
100-101	23.13056459765715	30.80788660494969	25.176105892251645	20.88544290514152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2224.0
1	2177.0
2	2556.0
3	3284.0
4	4044.5
5	5266.5
6	6724.5
7	9647.5
8	12227.0
9	12538.0
10	11809.0
11	11053.0
12	11785.5
13	14723.5
14	21557.0
15	30282.5
16	35772.0
17	36824.0
18	35325.5
19	32604.0
20	30017.0
21	28976.0
22	30405.5
23	35298.5
24	43752.5
25	56446.5
26	73328.0
27	91387.0
28	109497.5
29	128592.5
30	146184.5
31	163669.5
32	183070.5
33	203024.0
34	222313.0
35	240480.5
36	262036.0
37	280569.5
38	292294.5
39	301700.0
40	312064.0
41	322726.0
42	330238.0
43	346179.5
44	406914.0
45	449547.0
46	427954.0
47	402416.5
48	380294.0
49	363176.0
50	350993.0
51	340477.0
52	324316.0
53	302811.0
54	285964.5
55	267035.5
56	247248.0
57	225395.0
58	201612.0
59	176381.5
60	149555.5
61	124320.0
62	100040.5
63	80150.0
64	64483.5
65	50693.5
66	38452.0
67	26863.0
68	18597.5
69	14014.5
70	10288.0
71	6956.0
72	4749.5
73	3119.5
74	1953.5
75	1158.0
76	713.5
77	455.0
78	280.5
79	184.0
80	133.0
81	90.0
82	65.5
83	50.0
84	32.5
85	19.5
86	13.0
87	7.5
88	2.5
89	1.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0024340636947171933
4	0.0
5	0.04920038700459162
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.92544089587491E-4
28-29	2.713629469600335E-4
30-31	0.0
32-33	0.0
34-35	8.72766626567889E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	7.315011140457174E-5
48-49	0.0
50-51	1.863024710041939E-5
52-53	0.0
54-55	0.001067012286046674
56-57	0.0020514542963377975
58-59	0.008624684412764146
60-61	0.007807871282629935
62-63	0.004009146102404615
64-65	0.004961970937311666
66-67	0.00685152733533939
68-69	0.003763098833890933
70-71	0.0057230782084398
72-73	9.824447721145137E-4
74-75	0.0016487601323804497
76-77	0.0012474921261974572
78-79	0.0011809726067571898
80-81	9.586341969890038E-5
82-83	0.0
84-85	0.0
86-87	2.746035220952859E-4
88-89	9.185354746890988E-4
90-91	0.0
92-93	0.0
94-95	1.0424658905160623E-4
96-97	3.3361593043733546E-4
98-99	8.412091540380143E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	268.0
20-21	719.0
22-23	1056.0
24-25	1366.0
26-27	3527.0
28-29	8269.0
30-31	22960.0
32-33	28592.0
34-35	38028.0
36-37	58362.0
38-39	67087.0
40-41	63983.0
42-43	70230.0
44-45	81231.0
46-47	80142.0
48-49	74292.0
50-51	67151.0
52-53	64897.0
54-55	70067.0
56-57	73629.0
58-59	75205.0
60-61	77236.0
62-63	79960.0
64-65	108318.0
66-67	119734.0
68-69	104368.0
70-71	136188.0
72-73	69193.0
74-75	70446.0
76-77	74057.0
78-79	77677.0
80-81	78399.0
82-83	73971.0
84-85	74260.0
86-87	76331.0
88-89	79644.0
90-91	81870.0
92-93	80285.0
94-95	84927.0
96-97	132081.0
98-99	691020.0
100-101	5347605.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.61831306438916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.29294997859898	76.40437108796318
2	6.706670155064463	11.350142296016802
3	1.6038159831895593	4.0713660888961565
4	0.5721626049070436	1.9366173770304245
5	0.29143530202964807	1.2330381812579783
6	0.1522040935541735	0.7727552182829177
7	0.08096006503103767	0.47954928899567545
8	0.06340940377771197	0.4292477424070953
9	0.046076324523263955	0.35090107680293464
>10	0.1842355321009944	2.5153143230338872
>50	0.004966893661183867	0.28112647977606725
>100	0.001088604680552091	0.15245804062754753
>500	0.0	0.0
>1k	2.505888134766407E-5	0.023112798909428214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1535846894394283E-5	0.0
2	1.1535846894394283E-5	5.767923447197141E-5	0.0	1.1535846894394283E-5	0.0
3	1.1535846894394283E-5	5.767923447197141E-5	0.0	1.1535846894394283E-5	0.0
4	1.1535846894394283E-5	1.1535846894394282E-4	0.0	1.1535846894394283E-5	0.0
5	1.1535846894394283E-5	1.1535846894394282E-4	0.0	1.1535846894394283E-5	0.0
6	4.614338757757713E-5	1.1535846894394282E-4	0.0	1.1535846894394283E-5	0.0
7	4.614338757757713E-5	1.1535846894394282E-4	0.0	1.1535846894394283E-5	0.0
8	4.614338757757713E-5	1.3843016273273138E-4	0.0	1.1535846894394283E-5	0.0
9	5.767923447197141E-5	2.3071693788788563E-4	0.0	1.1535846894394283E-5	0.0
10-11	5.767923447197141E-5	2.3071693788788563E-4	0.0	2.3071693788788565E-5	1.1535846894394283E-5
12-13	8.075092826075998E-5	2.3071693788788563E-4	0.0	4.037546413037999E-5	1.1535846894394283E-5
14-15	8.651885170795712E-5	3.345395599374342E-4	0.0	4.614338757757713E-5	2.3071693788788565E-5
16-17	1.2112639239113996E-4	3.518433302790256E-4	0.0	5.767923447197141E-5	2.3071693788788565E-5
18-19	1.3266223928553423E-4	3.9798671785660274E-4	0.0	5.767923447197141E-5	3.4607540683182846E-5
20-21	1.3843016273273138E-4	4.6143387577577126E-4	0.0	5.767923447197141E-5	3.4607540683182846E-5
22-23	1.3843016273273138E-4	4.960414164589541E-4	0.0	6.921508136636569E-5	4.6143387577577124E-5
24-25	1.672697799687171E-4	5.479527274837284E-4	0.0	8.651885170795712E-5	5.767923447197141E-5
26-27	1.9034147375750568E-4	5.652564978253198E-4	0.0	1.672697799687171E-4	6.921508136636569E-5
28-29	1.961093972047028E-4	5.767923447197142E-4	0.0	4.960414164589541E-4	7.498300481356283E-5
30-31	2.1341316754629422E-4	6.113998854028969E-4	0.0	0.002007237359624605	8.075092826075998E-5
32-33	2.1918109099349135E-4	6.113998854028969E-4	0.0	0.0052603461838437926	8.075092826075998E-5
34-35	2.4802070822947707E-4	7.152225074524455E-4	0.0	0.00952284161132248	8.075092826075998E-5
36-37	2.653244785710685E-4	8.132772060547969E-4	0.0	0.01568875177637622	8.075092826075998E-5
38-39	2.7686032546546277E-4	8.59420593632374E-4	0.0	0.024282957712699962	8.075092826075998E-5
40-41	2.88396172359857E-4	9.747790625763168E-4	0.0	0.0334885635344266	8.075092826075998E-5
42-43	2.9993201925425134E-4	9.978507563651053E-4	0.0	0.04208853739419754	8.075092826075998E-5
44-45	3.0569994270144847E-4	0.001147816765992231	0.0	0.051346054526948945	8.075092826075998E-5
46-47	3.114678661486456E-4	0.0012747110818305683	0.0	0.06223012607180996	8.075092826075998E-5
48-49	3.172357895958427E-4	0.0013150865459609481	0.0	0.07286040898499428	9.228677515515426E-5
50-51	3.230037130430399E-4	0.0015400355604016365	0.0	0.08276970146727897	9.228677515515426E-5
52-53	3.460754068318285E-4	0.0015458034838488337	0.0	0.09297315804537072	9.228677515515426E-5
54-55	3.460754068318285E-4	0.0015458034838488337	0.0	0.1028939863745498	9.80546986023514E-5
56-57	3.518433302790256E-4	0.0015688751776376225	0.0	0.11076143395652671	1.0382262204954855E-4
58-59	3.5761125372622273E-4	0.0015746431010848195	0.0	0.11735993838012022	1.0382262204954855E-4
60-61	3.6337917717341986E-4	0.001597714794873608	0.0	0.12329513160728608	1.0382262204954855E-4
62-63	3.922187944094056E-4	0.001638090259003988	0.0	0.12920725314066317	1.0382262204954855E-4
64-65	3.922187944094056E-4	0.0018226638093142965	0.0	0.13469254833894764	1.0382262204954855E-4
66-67	3.922187944094056E-4	0.0018341996562086908	0.0	0.14050661517372237	1.0382262204954855E-4
68-69	4.0375464130379987E-4	0.0018688071968918736	0.0	0.1460438216830316	1.0382262204954855E-4
70-71	4.0375464130379987E-4	0.0019034147375750566	0.0	0.15167331496749603	1.0382262204954855E-4
72-73	4.0375464130379987E-4	0.001932254354811042	0.0	0.15629342164870091	1.1535846894394282E-4
74-75	4.0375464130379987E-4	0.002007237359624605	0.0	0.15993298134388234	1.1535846894394282E-4
76-77	4.0375464130379987E-4	0.002093756211332562	0.0	0.16315148262741835	1.1535846894394282E-4
78-79	4.0375464130379987E-4	0.0021456675223573366	0.0	0.1662027141309856	1.1535846894394282E-4
80-81	4.0375464130379987E-4	0.0021456675223573366	0.0	0.1672467082749283	1.1535846894394282E-4
82-83	4.0375464130379987E-4	0.0021514354458045336	0.0	0.16771967799759846	1.268943158383371E-4
84-85	4.0375464130379987E-4	0.0021572033692517307	0.0	0.1682676307250822	1.268943158383371E-4
86-87	4.09522564750997E-4	0.002174507139593322	0.0	0.16838875711747334	1.268943158383371E-4
88-89	4.2682633509258844E-4	0.0023417769195620396	0.0	0.16840029296436773	1.268943158383371E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	14125	0.0	56.799423	1
GGTATCA	9225	0.0	46.502514	1
TATACAC	19130	0.0	44.38648	3
TTATACA	18820	0.0	43.32455	2
GTATCAA	16575	0.0	34.89039	1
TACACAT	27470	0.0	30.987167	5
ACACATC	29890	0.0	27.671286	6
ATACACA	31760	0.0	27.611712	4
TCAACGC	22150	0.0	27.270218	4
ATCAACG	22320	0.0	27.043774	3
ACATCTC	30890	0.0	27.007484	8
TATCAAC	22715	0.0	26.424942	2
CAACGCA	22905	0.0	26.38975	5
CACATCT	32470	0.0	25.51153	7
AACGCAG	23780	0.0	25.474236	6
CATCTCC	34330	0.0	24.092476	9
ACGCAGA	28485	0.0	21.20734	7
CGCAGAG	30165	0.0	20.068151	8
ATCTCCG	27970	0.0	19.141127	7
TCTCCGA	28925	0.0	18.494581	8
>>END_MODULE