FastQCFastQC Report
Fri 27 May 2016
SRR2140179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9463430
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC133300.053.3649331
GGTATCA124850.048.0160031
TATACAC178600.042.902383
TTATACA184300.039.6742552
GTATCAA232150.036.6067621
TACACAT275700.028.0063345
ATCAACG320150.027.9819583
TCAACGC321600.027.8290964
CAACGCA329350.027.1486135
TATCAAC328250.027.0213812
ACACATC278300.026.8206356
AACGCAG341300.026.2586636
ACATCTC295450.025.4065888
ATACACA322700.025.0262244
CACATCT306600.024.2073967
GTGGTAT64300.023.4392381
TGGTATC61100.023.2849272
ACGCAGA403550.022.176747
CGCAGAG412250.021.7189648
CATCTCC345900.021.5058279
Produced by FastQC (version 0.11.5)