##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9463430
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.438727712890568	31.0	18.0	33.0	18.0	33.0
2	27.227716800356742	30.0	18.0	32.0	18.0	33.0
3	29.938373824289926	32.0	28.0	33.0	25.0	33.0
4	30.883315668843114	33.0	32.0	33.0	27.0	33.0
5	31.58695948509156	33.0	32.0	33.0	28.0	33.0
6	35.29719224424971	37.0	35.0	38.0	29.0	38.0
7	35.78556664972425	38.0	36.0	38.0	31.0	38.0
8	36.21679676396402	38.0	37.0	38.0	33.0	38.0
9	36.362973255997034	38.0	37.0	38.0	33.0	38.0
10-11	36.50466506330157	38.0	38.0	38.0	34.0	38.0
12-13	36.5262232615447	38.0	38.0	38.0	34.0	38.0
14-15	36.536900679774675	38.0	38.0	38.0	34.0	38.0
16-17	36.53108228200557	38.0	38.0	38.0	34.0	38.0
18-19	36.544491003790384	38.0	38.0	38.0	34.0	38.0
20-21	36.54090606842062	38.0	38.0	38.0	34.0	38.0
22-23	36.53089446885248	38.0	38.0	38.0	34.0	38.0
24-25	36.51942488983848	38.0	38.0	38.0	34.0	38.0
26-27	36.45935548130144	38.0	38.0	38.0	34.0	38.0
28-29	36.424142296364835	38.0	38.0	38.0	34.0	38.0
30-31	36.42160953519756	38.0	38.0	38.0	34.0	38.0
32-33	36.38570665552392	38.0	38.0	38.0	33.5	38.0
34-35	36.32426515117869	38.0	38.0	38.0	33.0	38.0
36-37	36.302755297684094	38.0	37.5	38.0	33.0	38.0
38-39	36.23764640273364	38.0	37.0	38.0	33.0	38.0
40-41	36.33125638389298	38.0	37.5	38.0	33.0	38.0
42-43	36.30134822784023	38.0	37.0	38.0	33.0	38.0
44-45	36.24436043012848	38.0	37.0	38.0	33.0	38.0
46-47	36.199989505388004	38.0	37.0	38.0	33.0	38.0
48-49	36.15197163823413	38.0	37.0	38.0	33.0	38.0
50-51	36.119610786582626	38.0	37.0	38.0	33.0	38.0
52-53	36.09586111736647	38.0	37.0	38.0	33.0	38.0
54-55	36.05060088176617	38.0	37.0	38.0	32.0	38.0
56-57	36.044074086601825	38.0	37.0	38.0	32.0	38.0
58-59	36.009877069510424	38.0	37.0	38.0	31.0	38.0
60-61	35.99167310174872	38.0	37.0	38.0	31.0	38.0
62-63	35.983336258693726	38.0	37.0	38.0	31.0	38.0
64-65	35.96447292698282	38.0	37.0	38.0	31.0	38.0
66-67	35.9574798215167	38.0	37.0	38.0	31.0	38.0
68-69	35.930372215355526	38.0	37.0	38.0	31.0	38.0
70-71	35.8952525758959	38.0	37.0	38.0	31.0	38.0
72-73	35.86205447715984	38.0	37.0	38.0	31.0	38.0
74-75	35.88596974776026	38.0	37.0	38.0	31.0	38.0
76-77	35.87100220874125	38.0	37.0	38.0	31.0	38.0
78-79	35.86191943937412	38.0	37.0	38.0	31.0	38.0
80-81	35.822788202822565	38.0	37.0	38.0	31.0	38.0
82-83	35.83060933108956	38.0	37.0	38.0	31.0	38.0
84-85	35.83366961286737	38.0	37.0	38.0	31.0	38.0
86-87	35.83341510550995	38.0	37.0	38.0	31.0	38.0
88-89	35.810480274149526	38.0	37.0	38.0	31.0	38.0
90-91	35.79846765194125	38.0	37.0	38.0	31.0	38.0
92-93	35.75358674306155	38.0	37.0	38.0	31.0	38.0
94-95	35.74991790055704	38.0	37.0	38.0	31.0	38.0
96-97	35.78731573811966	38.0	37.0	38.0	31.0	38.0
98-99	35.82941832881871	38.0	37.0	38.0	31.0	38.0
100-101	35.307696195453445	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	20.0
18	146.0
19	410.0
20	691.0
21	1252.0
22	3374.0
23	9156.0
24	19821.0
25	37247.0
26	60493.0
27	90508.0
28	127219.0
29	168399.0
30	213560.0
31	266450.0
32	332319.0
33	426982.0
34	579618.0
35	889819.0
36	1879407.0
37	4356539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.49089178025304	23.84055252693791	14.138858743605647	25.529696949203405
2	12.999462140048587	24.149246097873604	39.33413149354938	23.517160268528432
3	18.89822481843089	26.637223493403557	30.648318682018903	23.81623300614665
4	13.16512089168515	18.9559071076766	37.41465832155994	30.464313679078302
5	13.45722438113064	38.9617286275203	33.95110406342663	13.629942927922437
6	29.01398330203742	38.46262930036995	18.365043118615553	14.158344278977072
7	25.464139323691303	33.17553994693256	22.377182480348033	18.9831382490281
8	24.093853919773274	36.942292593700174	21.278976016095644	17.684877470430912
9	24.837548330784927	16.911363004745635	20.71979187250289	37.531296791966554
10-11	23.922335770434184	27.071859780227676	28.529275326176663	20.476529123161473
12-13	24.905240488913638	24.291895221922708	28.5799123573588	22.22295193180485
14-15	22.057404133596382	25.742790933097197	26.008857253659613	26.190947679646808
16-17	21.733488809025904	29.11501432355922	26.65187992091662	22.499616946498257
18-19	21.86764735407775	27.69472062455156	28.594045710698975	21.84358631067171
20-21	22.668070866659704	26.722217528522417	28.454150487272084	22.155561117545794
22-23	22.13090983953321	27.33427835599031	28.353950616383685	22.1808611880928
24-25	22.111009441659252	27.49264528916409	28.588397583623536	21.80794768555312
26-27	22.017151446579764	27.600717385324174	28.709262512747397	21.672868655348662
28-29	21.378289105372225	27.041463115228588	29.120111440065504	22.46013633933369
30-31	22.132968777607022	26.807641772730605	29.383317592694986	21.676071856967383
32-33	21.62729343759452	27.622318663316868	28.86672390108978	21.88366399799883
34-35	21.563379139860793	27.57216059128461	29.493490184281278	21.37097008457332
36-37	22.158933358476496	27.089214654749377	28.85167177030007	21.90018021647406
38-39	21.710265055851092	26.907681017763007	29.177669536015326	22.20438439037057
40-41	21.839147227372894	26.77400620343049	29.352305640239756	22.034540928956865
42-43	22.187242605231972	26.62183374282584	29.215123036343808	21.975800615598377
44-45	21.724096872576904	27.18206250773924	28.975381268703515	22.118459350980338
46-47	22.396382799008233	26.62120436996646	28.315809970044686	22.66660286098062
48-49	22.093889552743462	26.67144822651448	28.485261920273985	22.74940030046807
50-51	21.746646574339522	26.951833120929386	28.82055289154679	22.480967413184302
52-53	21.937095624662003	27.200780923746056	29.03671476977367	21.825408681818274
54-55	21.894338264087654	27.31961256540802	28.385271478659096	22.400777691845224
56-57	22.33339321912394	27.956826807662722	27.676057354958246	22.033722618255094
58-59	21.513035236946923	28.671152971856017	28.382696206109483	21.433115585087574
60-61	22.037934733182652	29.238250412611528	27.29821280400726	21.425602050198563
62-63	21.53730566486775	29.659894978295533	27.297636179598545	21.505163177238174
64-65	21.525348667097255	29.45198856600913	27.380915945925217	21.641746820968397
66-67	21.730032484526806	29.133094605081816	27.371059615033083	21.765813295358296
68-69	21.838846251904613	28.582188391453688	27.676713598577084	21.902251758064615
70-71	21.946239161357745	27.87121058727705	27.963614140510522	22.21893611085468
72-73	22.167074114152445	27.549958164817046	28.029831181363246	22.253136539667263
74-75	22.009706361445065	27.688338479142576	27.995911818921314	22.306043340491044
76-77	22.132162614127214	27.675258790370417	27.902588137860324	22.28999045764204
78-79	22.11629828341205	27.664785574873257	27.84505298197393	22.37386315974077
80-81	22.167739639595222	27.65576228854546	27.844222501627385	22.332275570231932
82-83	22.200758501550723	27.629963090224457	27.935802345811585	22.233476062413235
84-85	22.331052411526315	27.602389655405563	27.81484793164686	22.251710001421266
86-87	22.130344889715218	27.681027028841328	27.87698558767436	22.311642493769092
88-89	22.21758367497505	27.591652931090316	27.855988925484137	22.334774468450497
90-91	22.233821750540912	27.633634967944428	27.906379603128812	22.226163678385852
92-93	22.32405591841787	27.597024214381022	27.84566543321405	22.23325443398706
94-95	22.121145225942378	27.715167117822904	27.85673898445497	22.306948671779747
96-97	22.18115162613134	27.72832474374343	27.89723816762992	22.193285462495314
98-99	22.38561544579965	28.271041718749796	27.968742197148966	21.374600638301587
100-101	23.032130140980552	30.79377081540105	25.21371719700297	20.960381846615427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3521.0
1	3412.5
2	3513.0
3	4058.0
4	4999.0
5	6658.5
6	8510.0
7	11685.5
8	14371.0
9	14692.0
10	14006.0
11	13316.0
12	14576.5
13	18784.0
14	28046.0
15	39536.5
16	46642.0
17	47897.5
18	45241.5
19	40576.0
20	35722.0
21	32073.0
22	31443.0
23	35310.0
24	43757.0
25	56991.0
26	74545.0
27	93611.0
28	112991.5
29	134248.5
30	154126.5
31	174431.0
32	198013.0
33	222801.5
34	246177.5
35	268157.5
36	292063.5
37	312636.0
38	327255.0
39	339065.0
40	351403.5
41	363329.5
42	373514.5
43	390783.0
44	445711.5
45	484315.5
46	470422.5
47	450486.0
48	420202.5
49	394939.0
50	383341.0
51	369811.5
52	353053.0
53	334540.5
54	314530.5
55	291699.0
56	266735.5
57	240119.5
58	213649.0
59	186623.0
60	158012.0
61	127980.0
62	99236.0
63	75907.0
64	57236.0
65	42407.0
66	31424.5
67	23604.5
68	17776.0
69	13139.5
70	9665.5
71	6950.0
72	4952.0
73	3452.0
74	2322.0
75	1564.0
76	1054.5
77	682.5
78	460.5
79	314.5
80	195.0
81	114.5
82	78.5
83	56.5
84	40.5
85	28.0
86	20.5
87	18.0
88	14.5
89	7.5
90	3.5
91	1.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0024198414317007683
4	0.0
5	0.049548630887532315
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.510526278787185E-4
28-29	2.2742387038828023E-4
30-31	0.0
32-33	0.0
34-35	7.462048156327777E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.532970278266908E-6
46-47	1.1739934292146812E-4
48-49	0.0
50-51	1.709564678159918E-5
52-53	1.1491607162167147E-5
54-55	0.0010602248255988098
56-57	0.0020870921941699623
58-59	0.008598546662666209
60-61	0.007860353367612745
62-63	0.004081706232789534
64-65	0.0050173305180195625
66-67	0.00633385287405217
68-69	0.0035443594780971443
70-71	0.005724688874634541
72-73	0.0010020689815235936
74-75	0.001599572541985841
76-77	0.0014313002274250316
78-79	0.001206924868126788
80-81	8.768407078559667E-5
82-83	0.0
84-85	0.0
86-87	2.721445408502912E-4
88-89	0.001038013245472691
90-91	0.0
92-93	0.0
94-95	1.7607497624638524E-4
96-97	3.5731414993065504E-4
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	240.0
20-21	713.0
22-23	1225.0
24-25	1746.0
26-27	3881.0
28-29	9254.0
30-31	24706.0
32-33	31403.0
34-35	42040.0
36-37	64083.0
38-39	73643.0
40-41	71219.0
42-43	79931.0
44-45	92188.0
46-47	90811.0
48-49	83357.0
50-51	73738.0
52-53	70307.0
54-55	76476.0
56-57	79809.0
58-59	83834.0
60-61	91509.0
62-63	91668.0
64-65	109899.0
66-67	120101.0
68-69	107733.0
70-71	134707.0
72-73	76251.0
74-75	76563.0
76-77	80913.0
78-79	84880.0
80-81	86112.0
82-83	81431.0
84-85	81145.0
86-87	83469.0
88-89	87435.0
90-91	89875.0
92-93	87545.0
94-95	92996.0
96-97	143927.0
98-99	750611.0
100-101	5850056.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.51233303790646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81203782869399	76.80036889620332
2	7.187528109072574	12.29244594764656
3	1.7077922417054914	4.381118967968184
4	0.6149699711503082	2.103500679252675
5	0.27867532124723393	1.191508843996952
6	0.1465555540874214	0.7519384409807094
7	0.08182533784138071	0.489795287830195
8	0.04433036499688792	0.30326343482446644
9	0.031453331848960014	0.24206830093980525
>10	0.09243388178430297	1.2026411770698224
>50	0.0018439769982333102	0.10712805829051922
>100	5.152170993479537E-4	0.08041763568202673
>500	0.0	0.0
>1k	3.8863473858806845E-5	0.05380432931471526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0566993151531738E-5	0.0	1.0566993151531738E-5	0.0
3	0.0	1.0566993151531738E-5	0.0	2.1133986303063475E-5	0.0
4	0.0	1.0566993151531738E-5	0.0	5.2834965757658695E-5	0.0
5	0.0	1.0566993151531738E-5	0.0	5.2834965757658695E-5	0.0
6	0.0	1.0566993151531738E-5	0.0	5.2834965757658695E-5	1.0566993151531738E-5
7	0.0	3.170097945459521E-5	0.0	5.2834965757658695E-5	1.0566993151531738E-5
8	0.0	3.170097945459521E-5	0.0	5.2834965757658695E-5	1.0566993151531738E-5
9	0.0	6.340195890919043E-5	0.0	7.396895206072216E-5	1.0566993151531738E-5
10-11	0.0	6.340195890919043E-5	0.0	8.45359452122539E-5	1.0566993151531738E-5
12-13	0.0	6.340195890919043E-5	0.0	1.0566993151531739E-4	2.1133986303063475E-5
14-15	0.0	8.45359452122539E-5	0.0	1.1095342809108326E-4	4.226797260612695E-5
16-17	0.0	8.45359452122539E-5	0.0	1.1623692466684913E-4	5.2834965757658695E-5
18-19	0.0	9.510293836378564E-5	0.0	1.1623692466684913E-4	6.340195890919043E-5
20-21	0.0	1.1095342809108326E-4	0.0	1.1623692466684913E-4	6.340195890919043E-5
22-23	0.0	1.2680391781838085E-4	0.0	1.2680391781838085E-4	8.45359452122539E-5
24-25	0.0	1.3208741439414673E-4	0.0	1.532214006972102E-4	1.0566993151531739E-4
26-27	0.0	1.373709109699126E-4	0.0	3.117262979701863E-4	1.21520421242615E-4
28-29	0.0	1.373709109699126E-4	0.0	6.815710582737972E-4	1.3208741439414673E-4
30-31	0.0	1.373709109699126E-4	0.0	0.0019654607261849035	1.373709109699126E-4
32-33	0.0	1.4265440754567845E-4	0.0	0.004654760483249731	1.690718904245078E-4
34-35	0.0	1.5850489727297607E-4	0.0	0.008067899271194483	1.902058767275713E-4
36-37	0.0	1.690718904245078E-4	0.0	0.012928716120899082	1.9548937330333716E-4
38-39	0.0	1.7963888357603957E-4	0.0	0.020050869505031474	2.0077286987910304E-4
40-41	0.0	2.6417482878829347E-4	0.0	0.02778062499537694	2.1133986303063478E-4
42-43	0.0	2.8530881509135694E-4	0.0	0.0346597375370241	2.1133986303063478E-4
44-45	0.0	3.328602842732498E-4	0.0	0.04265366785615786	2.1662335960640064E-4
46-47	0.0	3.5927776715207914E-4	0.0	0.051255200281504695	2.2190685618216652E-4
48-49	0.0	3.5927776715207914E-4	0.0	0.059233280110911155	2.4304084248523E-4
50-51	0.0	4.015457397582061E-4	0.0	0.06724834441634799	2.4304084248523E-4
52-53	0.0	4.015457397582061E-4	0.0	0.07479317752654165	2.7474182193982523E-4
54-55	5.283496575765869E-6	4.1211273290973785E-4	0.0	0.08268672141073585	3.117262979701863E-4
56-57	1.0566993151531738E-5	4.2267972606126956E-4	0.0	0.09090255858605178	3.2757678769748384E-4
58-59	1.0566993151531738E-5	4.2267972606126956E-4	0.0	0.09832587127500282	3.381437808490156E-4
60-61	1.0566993151531738E-5	4.2267972606126956E-4	0.0	0.10524196829268034	3.5927776715207914E-4
62-63	1.0566993151531738E-5	4.3324671921280127E-4	0.0	0.11163499914935705	3.6984476030361085E-4
64-65	1.0566993151531738E-5	4.966486781219918E-4	0.0	0.1177268707012151	3.7512825687937673E-4
66-67	1.0566993151531738E-5	4.966486781219918E-4	0.0	0.12419915400652828	3.962622431824402E-4
68-69	1.0566993151531738E-5	5.336331541523527E-4	0.0	0.13041254597962895	4.121127329097378E-4
70-71	1.0566993151531738E-5	5.494836438796504E-4	0.0	0.13648328354518394	4.2267972606126956E-4
72-73	1.0566993151531738E-5	5.494836438796504E-4	0.0	0.14143391983667655	4.8608168497046E-4
74-75	1.0566993151531738E-5	5.547671404554162E-4	0.0	0.1451112334534096	4.966486781219918E-4
76-77	1.0566993151531738E-5	5.706176301827139E-4	0.0	0.1483605838475056	5.019321746977576E-4
78-79	1.0566993151531738E-5	5.811846233342457E-4	0.0	0.151488413820359	5.072156712735234E-4
80-81	1.0566993151531738E-5	5.811846233342457E-4	0.0	0.1527881539779974	5.230661610008211E-4
82-83	1.0566993151531738E-5	5.811846233342457E-4	0.0	0.15332178713214975	5.494836438796504E-4
84-85	1.0566993151531738E-5	5.811846233342457E-4	0.0	0.15377616783766562	5.494836438796504E-4
86-87	1.0566993151531738E-5	5.811846233342457E-4	0.0	0.1539822242041205	5.706176301827139E-4
88-89	1.0566993151531738E-5	6.023186096373091E-4	0.0	0.1540456261630297	5.759011267584798E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	13330	0.0	53.364933	1
GGTATCA	12485	0.0	48.016003	1
TATACAC	17860	0.0	42.90238	3
TTATACA	18430	0.0	39.674255	2
GTATCAA	23215	0.0	36.606762	1
TACACAT	27570	0.0	28.006334	5
ATCAACG	32015	0.0	27.981958	3
TCAACGC	32160	0.0	27.829096	4
CAACGCA	32935	0.0	27.148613	5
TATCAAC	32825	0.0	27.021381	2
ACACATC	27830	0.0	26.820635	6
AACGCAG	34130	0.0	26.258663	6
ACATCTC	29545	0.0	25.406588	8
ATACACA	32270	0.0	25.026224	4
CACATCT	30660	0.0	24.207396	7
GTGGTAT	6430	0.0	23.439238	1
TGGTATC	6110	0.0	23.284927	2
ACGCAGA	40355	0.0	22.17674	7
CGCAGAG	41225	0.0	21.718964	8
CATCTCC	34590	0.0	21.505827	9
>>END_MODULE