FastQCFastQC Report
Fri 27 May 2016
SRR2140178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10452381
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93550.040.014721
CTTATAC97150.034.2064671
GTATCAA161350.033.1927151
TCAACGC199650.027.9411874
ATCAACG202500.027.5063823
TATACAC145300.026.8845083
CAACGCA207650.026.8647145
AACGCAG216450.025.8182136
TATCAAC215450.025.5943322
TTATACA139750.024.853212
ACGCAGA258800.021.5270867
CGCAGAG266450.020.925118
TACACAT195800.019.380615
ATCTCCG144250.018.2414257
ACACATC193450.017.855526
ACATCTC197100.017.8510078
AGAGTAC289600.017.44702710-11
GCAGAGT327450.017.1317149
TCTCCGA154800.017.0535968
TACATGG263400.016.7024782
Produced by FastQC (version 0.11.5)