FastQCFastQC Report
Fri 27 May 2016
SRR2140177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7801194
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115800.053.7166331
GTATCAA217900.038.931311
TCAACGC261250.031.9887054
ATCAACG261650.031.9578023
TATCAAC268150.031.2801322
CAACGCA269500.031.057715
AACGCAG279550.029.959886
GTGGTAT55400.026.9162241
TGGTATC53950.026.6754682
ACGCAGA315200.026.5438447
CGCAGAG319200.026.197648
GCAGAGT367350.022.7402239
GAGTACT222000.021.25409712-13
CAGAGTA363300.019.98832310-11
GTACATG254050.019.8609121
TACATGG248150.019.8440112
GTACTTT244300.019.21675914-15
ACATGGG250050.019.1731593
AGAGTAC339200.018.75158310-11
AGTACTT241400.017.11398512-13
Produced by FastQC (version 0.11.5)