##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7801194
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.331305310443504	33.0	31.0	33.0	18.0	34.0
2	31.57332403219302	33.0	32.0	33.0	27.0	34.0
3	31.856163812872747	33.0	31.0	33.0	29.0	34.0
4	31.921226673763016	33.0	32.0	34.0	29.0	34.0
5	32.24106617525471	33.0	33.0	34.0	31.0	34.0
6	35.89311610504751	38.0	36.0	38.0	31.0	38.0
7	36.150701418270074	38.0	37.0	38.0	33.0	38.0
8	36.41295576036181	38.0	37.0	38.0	34.0	38.0
9	36.65741295499125	38.0	38.0	38.0	34.0	38.0
10-11	36.749946354365754	38.0	38.0	38.0	34.0	38.0
12-13	36.79642122731469	38.0	38.0	38.0	35.0	38.0
14-15	36.80194858633179	38.0	38.0	38.0	35.0	38.0
16-17	36.789098886657605	38.0	38.0	38.0	35.0	38.0
18-19	36.79847097764778	38.0	38.0	38.0	35.0	38.0
20-21	36.8023511930695	38.0	38.0	38.0	35.0	38.0
22-23	36.81220955656124	38.0	38.0	38.0	35.0	38.0
24-25	36.806262682646064	38.0	38.0	38.0	35.0	38.0
26-27	36.762756026053694	38.0	38.0	38.0	35.0	38.0
28-29	36.74489986332479	38.0	38.0	38.0	35.0	38.0
30-31	36.734628253060805	38.0	38.0	38.0	34.5	38.0
32-33	36.71388856085947	38.0	38.0	38.0	34.0	38.0
34-35	36.68146464939002	38.0	38.0	38.0	34.0	38.0
36-37	36.65552877886914	38.0	38.0	38.0	34.0	38.0
38-39	36.641880878981354	38.0	38.0	38.0	34.0	38.0
40-41	36.62864518837178	38.0	38.0	38.0	34.0	38.0
42-43	36.58948822531772	38.0	38.0	38.0	34.0	38.0
44-45	36.521469723723555	38.0	38.0	38.0	34.0	38.0
46-47	36.46890559899418	38.0	38.0	38.0	34.0	38.0
48-49	36.432851412113436	38.0	38.0	38.0	34.0	38.0
50-51	36.421554945886214	38.0	38.0	38.0	34.0	38.0
52-53	36.38581037648922	38.0	38.0	38.0	34.0	38.0
54-55	36.355149907428796	38.0	38.0	38.0	34.0	38.0
56-57	36.32454776730759	38.0	38.0	38.0	33.0	38.0
58-59	36.30375164607224	38.0	38.0	38.0	33.0	38.0
60-61	36.30374441881696	38.0	38.0	38.0	33.0	38.0
62-63	36.29157431583379	38.0	38.0	38.0	33.0	38.0
64-65	36.28161489721357	38.0	38.0	38.0	33.0	38.0
66-67	36.25659356659011	38.0	37.0	38.0	33.0	38.0
68-69	36.24960201761179	38.0	37.0	38.0	33.0	38.0
70-71	36.240244824151176	38.0	37.0	38.0	33.0	38.0
72-73	36.22226928704593	38.0	37.0	38.0	33.0	38.0
74-75	36.21302736213107	38.0	37.0	38.0	33.0	38.0
76-77	36.193665776902435	38.0	37.0	38.0	33.0	38.0
78-79	36.20298446439392	38.0	37.0	38.0	33.0	38.0
80-81	36.16038162701265	38.0	37.0	38.0	33.0	38.0
82-83	36.165504188581394	38.0	37.0	38.0	33.0	38.0
84-85	36.15221781880896	38.0	37.0	38.0	33.0	38.0
86-87	36.11744380995125	38.0	37.0	38.0	33.0	38.0
88-89	36.110682529365526	38.0	37.0	38.0	33.0	38.0
90-91	36.11356305316262	38.0	37.0	38.0	33.0	38.0
92-93	36.09563278698917	38.0	37.0	38.0	33.0	38.0
94-95	36.074039806211196	38.0	37.0	38.0	33.0	38.0
96-97	36.08001273754181	38.0	37.0	38.0	33.0	38.0
98-99	36.116314594478695	38.0	37.0	38.0	33.0	38.0
100-101	35.52711039623141	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	12.0
20	47.0
21	394.0
22	1652.0
23	4997.0
24	12181.0
25	22793.0
26	38026.0
27	56939.0
28	80733.0
29	107998.0
30	139218.0
31	175211.0
32	219823.0
33	283367.0
34	383659.0
35	580775.0
36	1160189.0
37	4533178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13635681922537	19.144582226771952	14.601892992277849	25.117167961724835
2	14.32649925126846	20.447639169081043	43.096531120748956	22.129330458901546
3	18.66201602121634	26.295751668967505	29.320128330740218	25.72210397907594
4	12.642513440891229	18.69317696752574	38.19576849389978	30.46854109768325
5	14.275705968350048	37.248595757777835	34.79312113673087	13.682577137141244
6	30.070819928334046	37.733082910128886	18.61981640246352	13.576280759073548
7	26.86939973547639	32.86639199076449	22.380151038417964	17.884057235341157
8	24.223317610099173	37.013898128927444	20.649505703870457	18.113278557102923
9	25.405175156520908	17.57165633876045	21.227058319534166	35.796110185184475
10-11	24.304503721995303	27.04222636745769	28.25663052518128	20.396639385365724
12-13	25.201870024071994	24.462379616014648	28.662787425958346	21.672962933955016
14-15	22.215791582673113	25.83509652496784	26.677377847544875	25.271734044814163
16-17	21.560000943445324	29.000746552386726	27.360446362441444	22.07880614172651
18-19	21.509003718469486	27.924368239295696	29.446103343033347	21.120524699201475
20-21	22.399157445079883	26.98859362819124	29.475635571154058	21.13661335557483
22-23	22.126259297062706	26.86774525285421	29.34234907087702	21.663646379206064
24-25	21.838716172595223	27.025435863470676	29.441250252329105	21.694597711605
26-27	21.761990128117002	27.155051343421494	29.389948610737026	21.693009917724478
28-29	21.646984590886344	27.23253976062365	29.41340132514247	21.707074323347538
30-31	22.010296638186148	27.043426906065644	29.333928193145418	21.61234826260279
32-33	21.74510241761815	27.2147200549088	29.294200438210495	21.745977089262556
34-35	21.890061110973566	27.28895360352554	29.263964280409	21.557021005091894
36-37	21.887358051258467	27.15807318159953	29.268000233467156	21.686568533674855
38-39	21.74270508708353	27.326922701573814	29.28454226657905	21.6458299447636
40-41	22.001195920258805	27.163037490863225	29.182280197297395	21.653486391580575
42-43	21.881482219013957	27.232207885117727	28.995404382470646	21.890905513397673
44-45	21.95022082804563	27.38787884135416	28.824546506904536	21.837353823695675
46-47	21.959095816343233	27.349779711393218	28.606136069182973	22.084988403080573
48-49	21.89411547729743	27.480297973101877	28.609354709905144	22.016231839695553
50-51	21.918367674499013	27.62097755956418	28.437340260599488	22.023314505337318
52-53	22.101008711936174	27.551391136821067	28.264370202053996	22.083229949188762
54-55	22.009849402662244	27.487110645905847	28.387940385259714	22.115099566172198
56-57	22.042407666340225	27.55328198482364	28.310253894531677	22.094056454304457
58-59	22.01164019766152	27.54437903114935	28.381590927660334	22.0623898435288
60-61	22.108368750282647	27.494882053000108	28.41211142028788	21.984637776429366
62-63	21.98649343289595	27.591731557404685	28.38663215543625	22.035142854263114
64-65	21.950826465965527	27.5431815538593	28.379768503411878	22.126223476763293
66-67	22.015941594603706	27.641078438980315	28.197283219035523	22.14569674738045
68-69	21.98747845160432	27.647520078554482	28.20573237338142	22.159269096459774
70-71	21.973567739979156	27.40865505414006	28.273542408335377	22.344234797545408
72-73	21.996870356147312	27.464031754955343	28.203523411459898	22.335574477437447
74-75	21.919534191303725	27.574006387170996	28.173995708281108	22.332463713244174
76-77	22.115817906298084	27.48061460365144	28.09227387634619	22.31129361370429
78-79	22.094492759619612	27.533190020511217	27.991353466492967	22.3809637533762
80-81	22.107817271065116	27.59941368604149	27.95854289258718	22.334226150306215
82-83	22.109761148941654	27.567002808565768	28.035182443504198	22.288053598988387
84-85	22.281225523791353	27.57350997671899	27.945315357923473	22.19994914156618
86-87	22.08559126250591	27.652573288151032	28.010273544791993	22.251561904551068
88-89	22.182118156995955	27.639629819768906	27.96446715750997	22.21378486572517
90-91	22.22649413657557	27.64612469607679	27.97496433965007	22.152416827697568
92-93	22.28531367626559	27.679624835547255	27.92439417731189	22.110667310875264
94-95	22.193087673296326	27.75008290031775	27.966259199294345	22.090570227091575
96-97	22.167396434138322	27.76188936117141	27.970232742896123	22.100481461794143
98-99	22.4180537053178	28.25897663102998	28.07537401443395	21.247595649218265
100-101	22.958864894600588	30.788593309246533	25.477121307868128	20.77542048828476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2208.0
1	1995.0
2	2340.5
3	2984.0
4	3609.5
5	5227.5
6	6952.5
7	9206.5
8	11966.5
9	13257.5
10	12428.0
11	11339.0
12	12071.0
13	14971.5
14	20892.0
15	28284.0
16	34323.0
17	38037.5
18	38560.0
19	35998.0
20	32222.5
21	28917.0
22	27819.0
23	30440.5
24	36700.0
25	46704.0
26	60323.0
27	74835.0
28	90260.0
29	107624.0
30	124931.5
31	142652.5
32	161593.5
33	181502.5
34	200336.5
35	217268.0
36	237829.0
37	255248.5
38	265249.5
39	273921.0
40	284796.5
41	295536.5
42	302217.5
43	308209.5
44	314654.5
45	319584.5
46	321443.0
47	322146.5
48	322019.5
49	318515.0
50	312276.5
51	303301.0
52	290177.5
53	275029.5
54	259890.5
55	241861.5
56	220747.0
57	199013.5
58	176257.5
59	152098.0
60	127446.5
61	103445.5
62	81019.0
63	61980.5
64	46453.5
65	34593.5
66	25782.5
67	19602.5
68	14928.5
69	11019.0
70	8177.0
71	5815.0
72	4027.0
73	2753.0
74	1782.0
75	1153.5
76	723.0
77	412.0
78	259.5
79	167.0
80	100.0
81	65.5
82	46.0
83	29.5
84	21.0
85	19.0
86	14.0
87	10.5
88	5.5
89	3.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.005294061396242678
4	0.0
5	0.04350616072360205
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.5637101192458488E-5
12-13	1.410040565585217E-4
14-15	0.0
16-17	0.0
18-19	6.409275298114622E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.98049583577111E-5
46-47	9.970133094585449E-4
48-49	2.6992999433079528E-5
50-51	1.0201547901266155E-4
52-53	0.0015545902942339493
54-55	7.995364342894434E-4
56-57	0.004129234061087125
58-59	0.009050384714731874
60-61	0.01160041412140175
62-63	0.00661486160673285
64-65	0.008725458040059007
66-67	0.006057565914249673
68-69	0.0030116106319166567
70-71	0.00515267969805297
72-73	0.0032358747587625746
74-75	0.0014561910522660573
76-77	0.002305422527067054
78-79	9.050773852572933E-4
80-81	3.610483800220101E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.2035802956502876E-4
94-95	3.631260065398994E-4
96-97	1.5882354219632228E-4
98-99	5.261039407168831E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	91.0
20-21	361.0
22-23	703.0
24-25	1056.0
26-27	2146.0
28-29	4756.0
30-31	13678.0
32-33	17869.0
34-35	24563.0
36-37	38729.0
38-39	45835.0
40-41	46089.0
42-43	53269.0
44-45	63324.0
46-47	63978.0
48-49	59308.0
50-51	53197.0
52-53	45937.0
54-55	49080.0
56-57	48874.0
58-59	55564.0
60-61	55036.0
62-63	52979.0
64-65	56447.0
66-67	59749.0
68-69	61249.0
70-71	60564.0
72-73	56897.0
74-75	58291.0
76-77	61606.0
78-79	64675.0
80-81	65157.0
82-83	60929.0
84-85	59751.0
86-87	62722.0
88-89	65581.0
90-91	68214.0
92-93	67217.0
94-95	72124.0
96-97	116764.0
98-99	662035.0
100-101	5224800.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.1894407671566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.61908336479664	75.44980510547416
2	7.205674748124793	12.132834547892967
3	1.7045983139660634	4.3052753635632275
4	0.642462137169675	2.163541121695488
5	0.29056430082549034	1.2231222996698947
6	0.1626731583005807	0.8217217335091817
7	0.09596190867308546	0.5655285598295214
8	0.06569517483182105	0.44246720241532767
9	0.04392307800166799	0.3328073436359364
>10	0.16505667741758084	2.1675670465323775
>50	0.003213260807106151	0.17091629135264536
>100	0.0010149677274168887	0.14768286831639857
>500	2.630311938760601E-5	0.019952148654185486
>1k	5.260623870435945E-5	0.05677836745868421
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.1274202384916975E-5	0.0	0.0	0.0
3	1.2818550596229244E-5	5.1274202384916975E-5	0.0	0.0	0.0
4	1.2818550596229244E-5	1.5382260715475092E-4	0.0	0.0	0.0
5	1.2818550596229244E-5	1.6664115775098017E-4	0.0	0.0	0.0
6	1.2818550596229244E-5	1.7945970834720942E-4	0.0	0.0	0.0
7	1.2818550596229244E-5	1.9227825894343865E-4	0.0	0.0	0.0
8	1.2818550596229244E-5	2.3073391073212638E-4	0.0	0.0	0.0
9	1.2818550596229244E-5	5.25560574445399E-4	0.0	0.0	0.0
10-11	1.9227825894343865E-5	5.25560574445399E-4	0.0	3.845565178868773E-5	0.0
12-13	2.5637101192458488E-5	5.25560574445399E-4	0.0	3.845565178868773E-5	0.0
14-15	2.5637101192458488E-5	7.178388333888377E-4	0.0	3.845565178868773E-5	6.409275298114622E-6
16-17	2.5637101192458488E-5	7.30657383985067E-4	0.0	3.845565178868773E-5	1.2818550596229244E-5
18-19	3.845565178868773E-5	7.819315863699839E-4	0.0	6.409275298114622E-5	1.2818550596229244E-5
20-21	3.845565178868773E-5	8.652521652454739E-4	0.0	7.691130357737546E-5	1.2818550596229244E-5
22-23	3.845565178868773E-5	9.67800570015308E-4	0.0	7.691130357737546E-5	2.5637101192458488E-5
24-25	3.845565178868773E-5	0.0010383025982945687	0.0	1.2818550596229244E-4	3.845565178868773E-5
26-27	5.1274202384916975E-5	0.001070348974785142	0.0	2.3073391073212638E-4	3.845565178868773E-5
28-29	5.1274202384916975E-5	0.0010767582500832565	0.0	5.768347768303159E-4	4.4864927086802356E-5
30-31	5.1274202384916975E-5	0.0012049437560455489	0.0	0.001794597083472094	5.1274202384916975E-5
32-33	6.409275298114622E-5	0.0012177623066417782	0.0	0.004390353579208516	5.1274202384916975E-5
34-35	7.691130357737546E-5	0.0013972220149889875	0.0	0.00812696107800934	5.1274202384916975E-5
36-37	7.691130357737546E-5	0.0015895002739324264	0.0	0.013914536672206844	6.409275298114622E-5
38-39	7.691130357737546E-5	0.001627955925721114	0.0	0.02566914756894906	6.409275298114622E-5
40-41	8.972985417360471E-5	0.0018394620105588965	0.0	0.06419530138591606	6.409275298114622E-5
42-43	8.972985417360471E-5	0.0019099640388381572	0.0	0.07555253721417517	6.409275298114622E-5
44-45	8.972985417360471E-5	0.002102242297781596	0.0	0.09157572545946172	6.409275298114622E-5
46-47	8.972985417360471E-5	0.0022240185284457736	0.0	0.10959219832246192	7.050202827926083E-5
48-49	8.972985417360471E-5	0.002243246354340118	0.0	0.12578202772549946	7.691130357737546E-5
50-51	8.972985417360471E-5	0.002544482293351505	0.0	0.1422859116181446	7.691130357737546E-5
52-53	8.972985417360471E-5	0.002576528669842078	0.0	0.17216595305795498	8.972985417360471E-5
54-55	9.613912947171934E-5	0.002595756495736422	0.0	0.24689810303397147	1.0254840476983395E-4
56-57	1.0254840476983395E-4	0.0026726677993137975	0.0	0.3289624639510311	1.0254840476983395E-4
58-59	1.0254840476983395E-4	0.0027047141758043706	0.0	0.4218713186725006	1.0254840476983395E-4
60-61	1.2177623066417782E-4	0.002762397653487402	0.0	0.44317574976343366	1.0254840476983395E-4
62-63	1.410040565585217E-4	0.002813671855872319	0.0	0.456391675428146	1.0254840476983395E-4
64-65	1.5382260715475092E-4	0.0032687304020384573	0.0	0.46669779010751433	1.0895768006794856E-4
66-67	1.6023188245286555E-4	0.0032815489526346864	0.0	0.4733121622151686	1.2177623066417782E-4
68-69	1.6664115775098017E-4	0.0033328231550196034	0.0	0.47968298186149455	1.2818550596229244E-4
70-71	1.6664115775098017E-4	0.0033840973574045204	0.0	0.48529750702264296	1.2818550596229244E-4
72-73	1.6664115775098017E-4	0.0033969159080007496	0.0	0.4893481690110514	1.2818550596229244E-4
74-75	1.6664115775098017E-4	0.003422553009193208	0.0	0.49278354057084084	1.2818550596229244E-4
76-77	1.6664115775098017E-4	0.003486645762174354	0.0	0.49573180720797355	1.2818550596229244E-4
78-79	1.6664115775098017E-4	0.0035122828633668128	0.0	0.49840447500728735	1.2818550596229244E-4
80-81	1.6664115775098017E-4	0.0035122828633668128	0.0	0.4995325074597555	1.3459478126040707E-4
82-83	1.6664115775098017E-4	0.0035315106892611567	0.0	0.5000196123824122	1.410040565585217E-4
84-85	1.6664115775098017E-4	0.0036084219928385324	0.0	0.5004682616532803	1.410040565585217E-4
86-87	1.6664115775098017E-4	0.003640468369329105	0.0	0.5006605399122237	1.410040565585217E-4
88-89	1.6664115775098017E-4	0.003768653875291398	0.0	0.5006925862887143	1.474133318566363E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11580	0.0	53.716633	1
GTATCAA	21790	0.0	38.93131	1
TCAACGC	26125	0.0	31.988705	4
ATCAACG	26165	0.0	31.957802	3
TATCAAC	26815	0.0	31.280132	2
CAACGCA	26950	0.0	31.05771	5
AACGCAG	27955	0.0	29.95988	6
GTGGTAT	5540	0.0	26.916224	1
TGGTATC	5395	0.0	26.675468	2
ACGCAGA	31520	0.0	26.543844	7
CGCAGAG	31920	0.0	26.19764	8
GCAGAGT	36735	0.0	22.740223	9
GAGTACT	22200	0.0	21.254097	12-13
CAGAGTA	36330	0.0	19.988323	10-11
GTACATG	25405	0.0	19.860912	1
TACATGG	24815	0.0	19.844011	2
GTACTTT	24430	0.0	19.216759	14-15
ACATGGG	25005	0.0	19.173159	3
AGAGTAC	33920	0.0	18.751583	10-11
AGTACTT	24140	0.0	17.113985	12-13
>>END_MODULE