FastQCFastQC Report
Fri 27 May 2016
SRR2140176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10229405
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147250.054.4107551
GTATCAA278950.040.294141
ATCAACG348450.031.8862253
TCAACGC351000.031.6405944
TATCAAC356700.031.390392
CAACGCA360750.030.7973885
AACGCAG377150.029.5364676
ACGCAGA415400.026.827127
GTGGTAT68150.026.308021
CGCAGAG437250.025.4668278
TGGTATC68050.024.8900132
GCAGAGT505950.022.0003229
GAGTACT285500.020.9801712-13
TACATGG342200.020.5164282
CAGAGTA479650.019.90166510-11
GTACATG361900.019.8997921
ACATGGG347850.019.737153
AGAGTAC451750.019.22367710-11
GTACTTT315050.018.95118514-15
AGTACTT304100.018.2024912-13
Produced by FastQC (version 0.11.5)